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Toxicity Activity and Total Phenolic Content of Soursop Leaves from Three Regions in South Sulawesi, Indonesia Rasyid, Faradiba Abdul; Amin, Asni; Sukmawati, Sukmawati; Djakariani, Kurnia Putri; Riska, Riska; Aliansyah, Moch. Rayhan; Sari, Cindy Artika
Journal of Experimental and Clinical Pharmacy (JECP) Vol 3, No 2 (2023): August 2023
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v3i2.674

Abstract

The fruit plant known as the soursop (Annona muricata L.) is indigenous to the Caribbean, Central America, and South America. The leaves of soursop contain substances with the potential to be cytotoxic, such as phenols and anonaceous acetogenins. Numerous variables, including geographic location, climatic conditions, and pest or disease disturbance factors, might impact a compound's phenol levels and toxicity. This study aims to investigate whether there are variations in the phenolic content and toxicity of the ethanol extract of soursop leaves that are grown in Gowa, Takalar, and Pinrang, three distinct regions in South Sulawesi, Indonesia. The Folin-Ciocalteu reagent was used to evaluate the total phenolic content, whereas Artemia salina Leach larvae were used for the BSLT method of the toxicity test. The results showed that the extracts of soursop leaves from Gowa, Pinrang, and Takalar had total phenolic contents of 3.7588% mg GAE/g, 0.9686% mg GAE/g, and 1.3832% mg GAE/g, respectively. Gowa, Pinrang, and Takalar have LC50 values of 38.19 µg/mL, 34.35 µg/mL, and 70.95 µg/mL, respectively, indicating their toxicity. The conclusion is that there were differences in the phenolic content and toxicity level of the ethanol extract of soursop leaves. All of these areas could source raw materials of soursop for the development of herbal medicines with cytotoxicity, i.e., anticancer, because they have LC50< 1,000 µg/mL.
Penyuluhan Tentang Bahaya Narkotika, Psikotropika dan Zat Aditif (NAPZA) Pada Siswa SMAN 5 Makassar Amin, Asni; Fawwaz , Muammar; Zakinah , Yuni; Faradiba; Abidin, Zainal
Mejuajua: Jurnal Pengabdian pada Masyarakat Vol. 4 No. 1 (2024): Agustus 2024
Publisher : Yayasan Penelitian dan Inovasi Sumatera (YPIS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/mejuajuajabdimas.v4i1.148

Abstract

Saat ini kasus penyalahgunaan Narkotika, Psikotropika, dan Zat Adiktif lainnya (NAPZA) di kalangan remaja sangat meningkat dan menjadi masalah nasional yang memerlukan perhatian khusus. Tujuan dilakukan pengabdian ini untuk mengedukasi, dan mengkaji pengetahuan dan sikap siswa SMA Negeri 5 Makassar tentang bahaya NAPZA. Metode pengabdian yang digunakan adalah metode kualitatif dengan pendekatan deskriptif berupa ceramah dan survey. Data dikumpulkan melalui kuesioner yang disebarkan kepada siswa sebelum dan sesudah penyuluhan. Hasil menunjukkan bahwa terdapat peningkatan pengetahuan siswa tentang bahaya NAPZA setelah mengikuti program penyuluhan. Selain itu, terdapat perubahan positif dalam sikap dan perilaku siswa terkait dengan penggunaan NAPZA. Kesimpulan dari penelitian ini adalah program penyuluhan efektif dalam meningkatkan kesadaran dan pemahaman siswa tentang bahaya NAPZA, serta sebagai salah satu upaya preventif akan penyalahgunaan NAPZA di kalangan remaja.
Skrining Farmakognosi Tanaman Etnofarmasi Asal Kabupaten Bulukumba yang Berpotensi sebagai Antikanker Asni Amin
Journal of Tropical Pharmacy and Chemistry Vol. 1 No. 4 (2012): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v1i4.36

Abstract

The use of traditional medicine has long been known and used by the people of South Sulawesi and recorded since the 15th century in lontara 'pabbura. Bulukumba as one of the areas of south Sulawesi inhabited by ethnic of Kajang and ethnic of Makassar has also been used plants to treat several of diseases including. Screening studies pharmacognostic origin Bulukumba conducted to identify medicinal plants used by the community Bulukumba to treat cancer. The research location is the village of Bahari Bonto Lembanna District Bulukumba, The research location is the village Lembana of Bonto Bahari District Regency of Bulukumba, the surveys plants etnofarmasi through sanro (traditional healers), public figures and society who have knowledge of traditional medicine with purposive sampling method. Pharmacognosy screening includes determination etnofarmasi plants that have been collected, organoleptic examination, morphology, anatomy and identification of chemical constituents using color reagent and precipitation reactions. The results obtained five plants etnofarmasi potential for the treatment of cancer, leaf of landep (Barleria prionitis L.) Family Acanthaceae, gewor (Basella rubra L) Famili Acanhtaceae, pecut kuda (Stachytarpheta jamaicensis) Family Loranthaceae, rumput mutiara (Hedyotis corymbosa L.) family Rubiaceae dan gondola (Commelina benghalensis L.) Family Commelinaceae, with chemical ingredients contain flavonoids, saponins, alkaloids, and tannins. The content of flavonoids and alkaloids in plants has the potential to be used as a cancer treatment. Keywords : Etnopharmacy, anticancer, Pharmacognosy screening. Abstrak Penggunaan obat tradisional telah lama dikenal dan digunakan oleh masyarakat Sulawesi Selatan dan dibukukan sejak abad ke-15 dalam lontara’ pabbura. Bulukumba sebagai salah satu wilayah Sulawesi Selatan dihuni oleh etnis Makassar dan etnis Kajang juga telah menggunakan tanaman termasuk untuk mengobati berbagai penyakit. Penelitian skrining farmakognostik asal Kabupaten Bulukumba dilakukan dengan tujuan untuk mengidentifikasi tanaman obat yang digunakan oleh masyarakat Bulukumba untuk mengobati penyakit kanker. Lokasi penelitian adalah desa Lembanna Kecamatan Bonto Bahari Kabupaten Bulukumba, dengan mensurvei tanaman etnofarmasi melalui sanro (pengobat tradisional), tokoh masyarakat dan masyarakat yang memiliki pengetahuan tentang obat tradisional dengan metode purposive sampling. Skrining farmakognosi meliputi determinasi tanaman etnofarmasi yang telah dikumpulkan, pemeriksaan organoleptik, morfologi, anatomi dan identifikasi kandungan kimia menggunakan pereaksi warna dan reaksi pengendapan. Hasil penelitian didapatkan lima tanaman etnofarmasi yang potensial untuk pengobatan kanker yaitu daun landep (Barleria prionitis L.) Famili Acanthaceae, gewor (Basella rubra L) Famili Acanhtaceae, pecut kuda (Stachytarpheta jamaicensis) Famili Loranthaceae, rumput mutiara (Hedyotis corymbosa L.) family Rubiaceae dan gondola (Commelina benghalensis L.) famili Commelinaceae, dengan kandungan kimia mengandung flavanoid, saponin, alkaloid, dan tanin. Kandungan flavonoid, dan alkaloid dalam tanaman berpotensi untuk digunakan sebagai obat kanker. Kata Kunci : Etnofarmasi, Antikanker, Skrining farmakognosi.
Hubungan Kemampuan Aktivitas Antioksidan dengan Pelarut Ekstraksi Daun Pecut Kuda (Stachytarpheta jamaicensis L.) Amin, Asni; Waris, Risda; Aminah, Aminah; Yuliana, Dewi; Fitri, Nurul
Journal of Pharmaceutical and Health Research Vol 5 No 3 (2024): October 2024
Publisher : Forum Kerjasama Pendidikan Tinggi (FKPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jharma.v5i3.5221

Abstract

The increasing prevalence of degenerative diseases, cancer and other non-communicable diseases is due to exposure to free radicals, so that currently research on antioxidants is increasingly being carried out. This research aims to prove the relationship between antioxidant activity and extraction solvents of different polarities in reducing DPPH radicals ((1,1-Diphenyl-2-picryl hydrazyl) from horse whip (DPK) leaves (Stachytarpheta jamaicensis L.) from North Kolaka district. The research was carried out using a post test only control experiment. Samples were extracted by maceration with 96% ethanol solvent and n-hexane solvent, then each extract was evaporated using a rotary evaporator, the thick extract was screened for phytochemicals with a second color reagent. The extract was tested for antioxidants against DPPH and measured absorbance using a UV-Vis spectrophotometer at a wavelength of 515 nm, and compared with the quercetin standard. The results of the phytochemical screening showed the content of anthraquinone glycosides, phenols, and flavonoids for the DPK ethanol extract, while the hexane extract contained alkaloids, phenols, flavonoids, tannins and steroids. The antioxidant activity of DPK extract can capture DPPH free radicals with the IC50 value of 96% ethanol extract being 12.17 μg/mL and hexane extract being 5.904 μg/mL. Both extracts are classified as very strong antioxidants like quercetin. There is a relationship between the extraction solvent and the antioxidant ability of HWL extract which is significant at p < 0.05, while the relationship between quercetin, ethanol extract and hexane extract significantly influences the antioxidant ability. Abstrak Meningkatnya prevalensi penyakit degeneratif, kanker, dan penyakit tidak menular lainnya diakibatkan adanya paparan radikal bebas, sehingga saat ini penelitian antioksidan semakin banyak dilakukan. Penelitian ini bertujuan untuk membuktikan adanya hubungan aktivitas antioksidan dengan pelarut ekstraksi yang berbeda kepolaran dalam meredam radikal DPPH ((1,1-Diphenyl-2-picryl hydrazyl) dari ekstrak etanol 96% dan ekstrak heksan daun pecut kuda (DPK) (Stachytarpheta jamaicensis L.) asal kabupaten Kolaka Utara. Penelitian dilaksanakan menggunakan post test only control eksperiment. Sampel diekstraksi secara maserasi dengan pelarut etanol 96%, dan pelarut n-heksan, kemudian tiap-tiap ekstrak dievaporasi menggunakan rotavapor, ekstrak kental diskrining fitokimia dengan pereaksi warna. Uji peredaman radikal bebas ekstrak dengan konsentrasi 1; 2; 3; 4 dan 5 μg/mL diukur absorbansinya dengan spektrofometer UV-Vis pada panjang gelombang 515 nm, dan dibandingkan dengan standar kuersetin. Hasil skrining fitokimia didapatkan kandungan glikosida antrakinon, fenol, flavonoid untuk ekstrak etanol DPK, sedangkan ekstrak heksan mengandung alkaloid, fenol, flavonoid, tanin, dan steroid. Aktivitas antioksidan ekstrak DPK dapat meredam radikal bebas DPPH dengan nilai IC50 ekstrak etanol 96% adalah 12,17 μg/mL dan ekstrak heksan adalah 5,904 μg/mL. Kedua ekstrak tergolong antioksidan sangat kuat seperti kuersetin. Terdapat hubungan antara pelarut ekstraksi dengan kemampuan antioksidan ekstrak DPK yang signifikan pada p < 0,05, sedangkan hubungan antara kuersetin, ekstrak etanol dan ekstrak heksan secara signifikan berpengaruh dengan kemampuan antioksidan.
PENGOLAHAN SAMPAH ORGANIK DAN ANORGANIK SEBAGAI IMPLEMENTASI P5 TEMA GAYA HIDUP BERKELANJUTAN PADA SISWA SMPIT AL-FIKRI MAKASSAR Effendi, Nurmaya; Amin, Asni; Nontji, Maimuna; Putra, Muhammad Alfinur; Pertiwi, Ermy; Alnamira, Rezki
Jubaedah : Jurnal Pengabdian dan Edukasi Sekolah (Indonesian Journal of Community Services and School Education) Vol. 4 No. 3 (2024): Jurnal Pengabdian dan Edukasi Sekolah (Jubaedah)
Publisher : LPPM Universitas Bina Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46306/jub.v4i3.240

Abstract

The Pancasila Student Profile Strengthening Project (P5) is a cross-disciplinary learning to observe and think of the problem solutions in the surrounding environment using a project-based learning approach. This community service aims to improve the character building and competency of SMPIT Al-Fikri students in implementing the P5 in sustainable lifestyle theme in terms of processing organic and inorganic wastes into valuable products. The products of organic waste processing include eco-enzymes, bar soap- and handwash-based eco-enzymes; inorganic waste processing products were ecobrics; and aromatherapy candles as products of used-cooking oil processing. As our partners, 132 students of SMPIT Al-Fikri, Makassar, attended this community service activity The method of this community service includes preparation and socialization; education and counseling in organic and inorganic waste processing; training and mentoring in making eco-enzymes, bar soap- and handwash-based eco-enzymes, ecobrics, and aromatherapy candles; activity evaluation through pre-posttest questionnaires. Our partners in this community service showed enthusiasm during discussions and practices, which are then able to improve their knowledge, attitudes, and skills in processing organic and inorganic wastes. Furthermore, we hope that our partners can apply their knowledge and skills in everyday life to to reduce environmental pollution and improve their quality of life
Teknik Ektraksi, Nilai Rendemen Dan Identifikasi Kandungan Metabolit Sekunder Ekstrak Daun Tembelekan (Lantana camara L) Asal Kota Kendari Hasninal, Sitti; Amin, Asni; Mirawati, Mirawati
JURNAL KESEHATAN POLTEKKES KEMENKES RI PANGKALPINANG Vol 12, No 2 (2024): JKP DESEMBER 2024
Publisher : Poltekkes Kemenkes Pangkalpinang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32922/jkp.v12i2.982

Abstract

Latar belakang: . Tembelekan merupakan salah satu tumbuhan yang berkhasiat sebagai obat tradisional, dan kosmetik, contohnya sediaan gel ekstrak daun tembelekan memiiliki aktivitas antioksidan yang kuat, antibakteri. Aktivitas farmakologi ditentukan oleh senyawa metabolit sekunder. Kandungan tersebut dapat diketahui dari suatu simplisia melalui identifikasi fitokimia. Tempat tumbuh dapat mempengaruhi kandungan kimia tanaman.Tujuan :  untuk mengetahui nilai rendemen dan identifikasi kandungan metabolit pada ekstrak daun tembelekan (EDT) hasil ekstraksi maserasi yang diambil dari Kota Kendari.Metode penelitian: daun tembelekan asal kota Kendari dibuat simplisia kering dan diekstraksi dengan teknik maserasi menggunakan pelarut etanol 70%. Ekstrak cair diuapkan dengan  rotavapor lalu hasil ekstrak kentalnya  diidentifikasi metabolit sekunder  dengan menggunakan reagen spesifik. Nilai rendemen dihitung dari ekstrak kental yang diperoleh.Hasil: nilai rendemen maserat  EDT  adalah 5,42%. identifikasi metabolit sekunder (IMS)  eksrak daun tembelekan didapatkan  senyawa alkaloid, flavonoid, saponin dan tanin.  Kesimpulan: nilai rendemen EDT hasil maserasi dan kandungan metabolit sekundernya dapat dijadikan data referensi dalam  pengembangan  obat alam dari EDT. Kata kunci: ekstrak daun tembelekan, Kendari, metabolit sekunder, nilai rendemen.
Inventarisasi Tanaman Obat untuk Penderita Penyakit Tidak Menular di Kota Makassar Asni Amin; Faradiba Faradiba
Makassar Natural Product Journal (MNPJ) April 2023
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Makassar City is one of the largest cities in the eastern region of Indonesia with the lifestyle of its society having undergone changes, starting from consuming instant food, lack of movement, and rarely exercising. So that, there is an increase in non-infectious diseases. People in the city of Makassar use traditional medicinal plants in accordance with the changing trend of people who return to nature "back to the nature". Yhe purpose of this study, we wanted to find out how the use and the process and the application of traditional medicine regarding its types, morphology, and its properties in Makassar society. Furthermore, as well as to get the suitability of scientific data by community use. The research method used was a descriptive survey with a qualitative approach, a combination method of purposive sampling and snowball sampling was used in determining the sample, the data was collected by direct observation and in-depth interviews. The results of research that have been carried out obtained about 47 types of plants, the part of the plant that used often by the community is leaves. And there are also the rhizomes, bark, fruit skin, stems, fruit, tubers, seeds, and herbs. Moreover, the processing of traditional medicine was boiled, brewed, pounded, squeezed, and without processing, while the application of use is by drinking, rubbing on the affected area, smearing, patching, and consume immediately. Based on data from various literature, some medicinal plants that have been used by society have scientific evidence as to their activities. The people of Makassar city use traditional medicinal plants in the treatment of non-infectious diseases and also there is scientific evidence on some plants that supports the use of traditional medicines by society.
RASIO NILAI RENDAMEN dan LAMA EKSTRAKSI MASERAT ETANOL DAGING BUAH BURAHOL (Stelecocharpus burahol) BERDASARKAN CARA PREPARASI SIMPLISIA Asni Amin
Makassar Natural Product Journal (MNPJ) Oktober 2023
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Introduction : Burahol fruit (Stelecocharpus burahol) is classified as a drupa fruit with thick, fibrous flesh, in Indonesia the fruit is used by the daughters of the Yogyakarta and Surakarta palaces as a deodorant for body odor and bad breath. In order to be extracted, proper simplicia preparation techniques are needed. The purpose of this study is to compare the yield values and the duration of the burahol pulp extraction process based on the simplicia preparation method. Research methods is a laboratory experiment. The simplicia was prepared in two ways, namely the wet preparation method (wet simplicia/SB), and the simplicia drying method (dry simplicia/SK) from burahol fruit flesh (DBB). Extraction of each simplicia using ethanol solvent with maceration method. Each liquid macerate (SB and SK) was evaporated to obtain a thick extract, and the yield value was calculated.Research result : the yield value of DBB extract for SB was 11.72%, and SK was 23.408%. The time for DBB extraction by maceration for SB was longer with 9 times remaceration, while for SK it was 6 times remaceration. Conclusion: the yield value of the extract from SK is higher than that of SB
Pharmacognostic Identification and Specific Quality Parameters of Blue Porterweed (Stachytarpheta jameicensis) Leaf Extract from Enrekang Regency, South Sulawesi amin, asni; Risda Waris; Noer Kurnia; Nur Fadilla
Jurnal FARMASIMED (JFM) Vol 7 No 2 (2025): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v7i2.2477

Abstract

The leaves of Blue Porterweed (Stachytarpheta jamaicensis) are commonly found in Enrekang district and used as medicinal plants. Its pharmacological effects have been tested invitro and invivo. However, data on the quality of this extract is still unpublished. This study was conducted to identify the pharmacognostic and specific quality parameters of horse whip leaf extract from Enrekang district. The research method is laboratory experimental. Pharmacognostic identification was carried out by identifying plant morphology, macroscopic test of simplisia, and identification of chemical content by Thin Layer chromatography (TLC). The extract obtained from maceration using 70% ethanol and then determined its specific parameters including organoleptic tests, extract identity parameters, soluble extract content in certain solvents. The results of pharmacognostic identification that have been carried out show that horse whip is a shrub, 50 cm-1.2 m high, taproot, lanceolate leaf, serrated edges, pinnate leaf repetition and belongs to the Verbenaceae family, and the TLC results of the extract show the presence of alkaloid, phenol, and flavonoid compounds. The identity name of the extract is Stachytarpetae Folium Extractum, black brown in colour, thick extract texture, slightly bitter taste, and weak odour. Test results of water soluble extract content = 14.96% and ethanol soluble extract content = 15.23%. Conclusion: Data on specific quality parameters of blue porterweed leaf extract will be a reference for the manufacture of standardised herbal medicine.
PKM Penyuluhan Rokok dan Bahayanya pada Sekolah Menengah Atas (SMA) di Makassar Abidin, Zainal; Faradiba, Faradiba; Amin, Asni; Fawwaz, Muammar; Olii, Audia Triani; Ririn, Ririn; Razak, Rais; Yulianto, Yuni Zakinah
Jurnal Pengabdian Masyarakat Bhinneka Vol. 3 No. 4 (2025): Bulan Juli
Publisher : Bhinneka Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58266/jpmb.v3i4.198

Abstract

Perilaku merokok di kalangan remaja, khususnya siswa sekolah, menjadi perhatian serius. Data menunjukkan bahwa usia perokok pemula di kelompok usia 13-15 tahun meningkat, bahkan, pada kelompok usia 15-19 tahun dan pada usia 10-14 tahun, hal ini  mengindikasikan bahwa kebiasaan merokok telah menyebar di kalangan siswa sekolah menengah pertama dan atas. Dampak merokok pada kesehatan remaja sangat serius, termasuk risiko kanker, gangguan jantung, diabetes, dan kematian. Faktor yang mendorong remaja merokok antara lain pengaruh teman sebaya, keluarga, iklan, dan kurangnya kesadaran tentang bahaya merokok. Dalam upaya pencegahannya, berbagai program anti-rokok, seperti kampanye di sekolah, media massa, dan kegiatan penyuluhan telah dilakukan. Salah satunya adalah program penyuluhan oleh Fakultas Farmasi Universitas Muslim Indonesia di SMA Negeri 5 Makassar, yang bertujuan meningkatkan pengetahuan siswa tentang bahaya merokok serta mengurangi minat merokok di kalangan remaja. Hasil dari pelaksanaan penyuluhan yang dilakukan memberikan dampak positif terhadap pengetahuan, sikap dan perilaku siswa SMA Negeri 5 Makassar, di mana hasil dari data kuisioner yang dilakukan, sebelum dan sesudah penyuluhan, mengalami peningkatan perubahan pengetahuan, sikap, dan perilaku siswa terhadap rokok, sehingga diharapkan siswa menjadi lebih mengurangi atau bahkan bisa berhenti merokok.