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Journal : Biocelebes

MEDIA DASAR LIMBAH DAUN KAKAO UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) Umrah; Saparudin, Saparudin; Yuniati, Eny; Ananda, Meryany
Biocelebes Vol. 17 No. 2 (2023)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v17i2.16606

Abstract

Cocoa leaf wastes as a basic medium are the nutrition that needed for the growth of white oyster mushroom mycelium (Pleurotus ostreatus). The objectives of this study were determine the cocoa leaf wastes as a basic medium for the growth of white oyster mushroom mycelium and the dosage of basic medium to encourage the best fungal growth. This research was designed in a Completely Randomized Design (CRD), consisting of seven treatments and three replications, namely P1 (Basic Media of 100% cocoa leaf waste), P2 (Basic Media 90% + Suplement 10%), P3 (Basic Media 80% + Suplement 20 %), P4 (Basic Media 70% + Suplement 30%), P5 (Basic Media 60% + Suplement 40%), P6 (Basic Media 50% + Suplement 50%), P7 (Sawdust 70%, 20% rice bran + flour corn 10%). The method that used in this study were media preparation, drying, grinding and formulation. The parameters that observed were the basic media, microscopic characteristics of hyphae, mycelium growth curve and macroscopic characteristics of colonies. The results of P1 and P7 showed the best growth for mycelium, observation of mycelium growth were done every 3 days. The isolation stage was carried out on Potato Sucrose Agar (PSA) media, the results obtained that P1 was 0,1 x 1012 CFU/g, P2 was 0,4 x 1012 CFU/g, P3 was 0,3 x 1012 CFU/g, P4 as much as 0,7 x 1012 CFU/g, P5 as much as 0,6 x 1012 CFU/g, P6 as much as 0,4 x 1012 CFU/g, P7 as much as 2,4 x 1012 CFU/g, respectively. Cocoa leaf waste are potentially as a growth medium for mycelium of white oyster mushroom.
Co-Authors ABD Aziz, ABD Abdul Quddus Afni, Nur' Ahyar Ahyar Alhuda, Fitri Amaludin, Amaludin Amuddin Amuddin Andayani, Melia Atmanda, Aulia Aulia, Hesti Dina Azlansyah, Azlansyah Baiq Rien Handayani, Baiq Rien Br Munthe, Winda Dahlan, Fathurrozi Defina, Azzira Welmi Desi Aneka Putri Diah Ajeng Setiawati Dio Andika Putra Eduar, Rudi Edwin Stiadi, Edwin Elya Antariksana Bachmida Emawati emawati Enny Yuliani Eny Yuniati Fadhlun, Fadhlun Fajri, Wahyu Fakhrul Irfan Khalil Farizal Farizal Firman Fajar Perdana Fitria Raudhatul Zannah Fuad Sauqi Isnain Gufron Gufron Hanifah Ayu HARTINI, INDRA Hary Kurniawan Hasanah, Titi Hendra Kurniawan Hestin, Hestin Hidayati Hidayati Hidayati, Baiq Titin I Gede Tunas Ince Siti Wardatullatifah S Irda Junita Ishak, Suhaimi Isnaini Puspitasari Jodion Siburian Joko Sumarsono Liswati, Liswati M.Azhar Mustafid Maksi Sirampun Markus, Markus Meryany Ananda Mi’raj Fuadi Mohamad Abdun Nasir Mohamad Iwan Fitriani, Mohamad Iwan Muhamad Hilmi, Muhamad Muhammad Habiburrahman, Muhammad Muhammad Pazuli Murad, Murad Nabila, Putri Maharani Nadila Aziz Neyla Vista Maramy NURUL HIDAYAH Nurul Yakin Nurwijayanti Opi Andriani Putra , Fibrian Khotmul Aula Rahmadania, Yerica Rahmat Nurcahyo Rahmat Sabani Reza Kusuma Nurrohman Rizal, Saiful Royan, M. Rusilawati, Tri Rustina, Desi Saharudin Saharudin Satrijo Saloko Soekardono Soekardono, Soekardono Sri Muryati Sukmawaty Sukmawaty Tentia, Ine Tri Isti Rahayu Umrah Umrah Wayan Sweca Yasa Wiwin Apriyanditra Yudhi Setiawan Yuhendra AP Zaenudin Zaenudin Zulfiana Jayanti