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Panjang Siklus Estrus dan Struktur Histologi Ovarium Tikus Putih Setelah Pemberian Ekstrak Etanol Daun Kaliandra Merah Kadek Mardika; Iriani Setyawati; Anak Agung Ketut Darmadi
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Red calliandra (Calliandra calothyrsus Meissn.) leaves contain chemical compounds such as flavonoids, tannins, and steroids. Previous research of C. calothyrsus leaf extracts showed effect on male mice reproduction, but no research has been done about its effect on estrous cycle and histology structure of albino rat ovary. This research aimed to study the potency of C. calothyrsus leaf extracts as phytoestrogen on the female reproductive organ through observing the estrous cycles, and histological structure and weight of the ovaries. Twenty four of 54-day-old female albino rats (Rattus norvegicus) Wistar strain were divided into four groups i.e. placebo control (given 0.5% Na-CMC solvent) and three treatment groups of C. calothyrsus leaf extracts in dosages 17.5; 35; and 70 mg/kg BW (respectively, group P1, P2 and P3). Treatments were administered orally in 1 ml/rat/day for 20 days. The vaginal smears were investigated from day 5th for 15 days (three estrous cycles). It was carried out every 8 hours/day. At the end of the treatments, rats were dissected for collecting the ovaries. They were weighed and prepared histologically then by using paraffin method with Hematoxylin-Eosin staining. The parameters observed were ovary weight and histological structures, including the number of primary follicles, secondary follicles, tertiary follicles, de Graff follicles, corpus luteum, and atretic follicles. The results showed that C. calohtyrsus leaf extracts have a phytoestrogenic potency by shortened the proestrous and estrous phases, increased the corpus luteum number and decreased the ovarian weights significantly at dose 70 mg/kg BW.
Penampilan Reproduksi dan Perkembangan Skeleton Fetus Mencit Setelah Pemberian Ekstrak Buah Nanas Muda Iriani Setyawati
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This experiment was performed to examine the effects of young pineapple (Ananas comosus) fruitextract on reproduction performance and foetus skeletal development given during organogenesis period.Twenty pregnant mice were randomly divided into four groups. The young pineapple fruit extract wastreated orally by gavage with doses 0% (control), 20%, 40%, and 80% since gestation day 6 to 15. Caesareansection were performed to pregnant mice on day 18 to remove foetuses. Observation covered : reproductionperformance (uterine weight before and after foetus removal), the foetus number (reabsorptions, dead/alive foetus), morphological of the foetus (weight and length of the litter, and malformations), and skeletaldevelopment (ossification number of metacarpus, metatarsus, vertebrae caudalis) and malformations ofcostae, sternebrae, and vertebrae. Statistical analysis was performed using Anova and Duncan’s MultipleRange Test.Teratogenic effects caused decreasing of earlier uterine weight and alive foetus, increasingdead foetus and hemorrhage, delayed skeletal ossification (decreasing ossification number of metacarpusand metatarsus) and caused costal malformation (intercostal fusion and convulated costal structure).Young pineapple fruit extract given to pregnant mice during organogenesis alter reproductive performancepregnant mice. The extract also caused morphological abnormalities (cretinism), hemorrhage, delayedossification on metacarpus and metatarsus, and costae malformation of the foetus.
Histologi Tubulus Seminiferus dan Kadar Testosteron Tikus yang Diberi Pakan Imbuhan Tepung Daun Kaliandra dan Kulit Nanas (HISTOLOGY OF SEMINIFEROUS TUBULES AND TESTOSTERONE LEVEL OF RAT GIVEN CALLIANDRA LEAF MEAL AND PINEAPPLE PEELS IN THE DIETS) Iriani Setyawati; I Gusti Ngurah Agung Dewantara Putra; Ni Gusti Ketut Roni
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.809 KB) | DOI: 10.19087/jveteriner.2017.18.3.369

Abstract

Calliandra calothyrsus leaf contains 17-28% protein which is hihly potential for use as source of protein supplement for animal feed. However, the plants also contain high level (>10%) of condensed tannins as antinutritional effects which may reduce consumtion efficiency of diet. The addition of protease (bromelain) into feed containing tannin is expected to decrease the negative effects of tannins. Bromelain can be obtained from the pineapple plant (Ananas comosus) including on the peels. This study was conducted to determine the effect of calliandra tannin combined with bromelain protease of pineapple peels in the feed on testicular histology and testosterone level of rats feeded during the growth period. The feeding experiment on post-weaning male rats was conducted using a completely randomized factorial design (4 main factors x 4 subfactors). The main factors were calliandra leaf meal substitution of 0; 10; 17.5 and 25% in the diets and subfactors were addition of pineapple peels, 0; 4.35; 8.70 and 13.05 g/rat/day. Rats were divided into 16 groups and were feeded the diets for two months (during the growth period). The results showed the susbtituion of feed with calliandra leaf had no sigbnificant effecton the thickness of the seminiferous tubules, but it appeared to have significant effect on the histology of seminiferous tubules. Increased pineapple peels level in the diets containing calliandra decreased wall thickness of the seminiferous tubules of the rat testes, indicating that bromalein fastened the maturation of spermatozoa. The addition of pineapple peels into the diet containing calliandra had a significant interaction effect on testoteron levels of male rats, but the testoteron levels among all treated rats were still within the normal range. ABSTRAK Daun kaliandra (Calliandra calothyrsus) mengandung protein 17-28% sehingga potensial sebagai sumber protein pakan ternak, namun tanaman ini mengandung condensed tannin cukup tinggi (>10%) yang bersifat antinutrisi. Penambahan enzim protease (bromelin) pada pakan yang mengandung tanin diharapkan dapat mengatasi dampak negatif tanin. Bromelin dapat diperoleh dari tanaman nanas (Ananas comosus) termasuk dari kulit buahnya. Penelitian ini bertujuan untuk mengetahui pengaruh tanin daun kaliandra dalam pakan yang dikombinasikan dengan protease bromelin dari limbah kulit nanas terhadap histologi testis dan kadar hormon testosteron tikus jika diberikan pada masa pertumbuhan. Penelitian ini merupakan percobaan pakan pada tikus jantan pascasapih selama masa pertumbuhan dengan Rancangan Acak Lengkap pola faktorial berjenjang 4x4 dengan level substitusi tepung daun kaliandra 0; 10; 17,5 dan 25% dalam ransum (main factor) dan dosis aditif kulit nanas 0; 4,35; 8,70 dan 13,05 g/ekor/hari (sub factor). Tikus dibagi menjadi 16 unit percobaan dan diberi perlakuan ransum selama dua bulan (masa pertumbuhan). Hasil penelitian menunjukkan bahwa tebal dinding tubulus seminiferus testis tikus tidak berbeda nyata, namun tampak adanya perbedaan pada gambaran histologi yang meliputi struktur membran basal, susunan antar sel-sel spermatogenik pada dinding tubulus, serta gambaran sel-sel spermatozoa di dalam lumen tubulus. Peningkatan dosis kulit nanas dalam ransum yang mengandung kaliandra menurunkan tebal dinding tubulus seminiferus. Aditif kulit nanas ke dalam ransum yang mengandung kaliandra menunjukkan interaksi yang memengaruhi kadar hormon reproduksi dengan menurunkan kadar hormon testosteron tikus jantan walaupun masih dalam kisaran normal.
AKTIVITAS HATI TIKUS (RATTUS SP) YANG DIBERI RANSUM MENGANDUNG TEPUNG DAUN KALIANDRA (Calliandra calothyrsus) DAN KULIT NANAS (Ananas comosus) SELAMA MASA PERTUMBUHAN IRIANI SETYAWATI; I GUSTI NGURAH AGUNG DEWANTARA PUTRA; NI GUSTI KETUT RONI
Jurnal Biologi Udayana Vol 21 No 1 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.7 KB) | DOI: 10.24843/JBIOUNUD.2017.vol21.i01.p07

Abstract

Calliandra calothyrsus leaves contain 17-28% protein thus potential as a protein source in animal diet. However, it also contain a high antinutrition of condensed tannins (>10%). The addition of protease enzymes (bromelain) in the diet containing tannins is expected to overcome the negative effects of tannins. Bromelain can be obtained from the pineapple plant (Ananas comosus) including the peel. This study was a feeding experiment on weaned male and female rats during the growth period. This study used a completely randomized design of 4x4 factorial design. The main factor was Calliandra leaf meal substitution levels of 0; 10; 17.5 and 25% in the diet. The sub factor was pineapple peel additive levels of 0; 4.35; 8.70 and 13.05 g/rat/day. Weaned rats were divided into 16 groups and they were fed for two months during growth period. The study showed that there was no interaction between calliandra leaves and pineapple peels on the rat liver activity including the blood levels of SGOT and SGPT levels. SGPT and SGOT levels were not affected by all levels of pineapple peels in the diets. All calliandra levels did not affect the level of SGOT, but 17.5 and 25% calliandra in the diets increased SGPT level.
Number of spermatogenic cells and testes histology of mice (Mus musculus L.) after treated with different times and intervals of red calliandra (Calliandra calothyrsus Meissn.) leaf extract Ni Made Estriana Pranadya; Iriani Setyawati; Dwi Ariani Yulihastuti
Jurnal Biologi Udayana Vol 23 No 1 (2019): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.422 KB) | DOI: 10.24843/JBIOUNUD.2019.v23.i01.p05

Abstract

Tannin compounds in the form of tanic acid are known to inhibit the acrosine activity of spermatozoa and plasminogen activator, which is an antifertility mechanism. Red Calliandra (Calliandra calothyrsus Meissn.) leaves have the potential to be developed as herbal medicines, but these leaves contain high condensed tannins, so research needs to be done to determine the effect of red kaliandra leaf extract on the reproduction of male mice.This research used a completely randomized design (CRD) in a factorial patern with four doses and three replications, i.e. control (NaCl 0,9%), doses of red caliandra leaf extract of 2, 4 and 6 mg/kg body weight and time interval of 7, 14 and 21 days. The testes organ were taken and processed histologically by Paraffin Methods and Hematoxylin-Eosin staining. Parameters observed were the amount of spermatogonia, spermatocyte and spermatid cells of male mice. A directly calculation method was done by observed the cells of tubulus seminiferous histology for each treatment used a microscope camera and Image Raster software (Optilab). The data were analyzed with One Way Anova and Duncan Multiple Range Test (DMRT). Calliandra calothyrsus leaf extract with different doses and time intervals decreased the amount of spermatogonia, spermatocytes and spermatids cells in the testes tubulus seminiferous of male mice, the arrangement of spermatogenic cells are increasingly irregular and the lumen were not full filled with spermatozoa cells.
MORPHOLOGY AND SKELETAL DEVELOPMENT OF RAT (Rattus norvegicus) FETUS AFTER FED DIETS CONTAINING Calliandra calothyrsus LEAF DURING GESTATION PERIOD Ruth Ellisa Christiani; Iriani Setyawati; Dwi Ariani Yulihastuti
Jurnal Biologi Udayana Vol 20 No 2 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This study aims to determine fetus morphology and skeleton development of rats after feddiet containingCalliandra leaf flour. This study used a completely randomized design with 16 pregnant rats that were dividedinto four treatment groups i.e. 0, 10, 17.5 and 25% of Calliandra leaf flour. The diets were given 20 g/rat/dayeach, twice a day, for 30 days (7 days of diet adaptation, 21 days of gestation period, and one day after birth).One day after the birth, all fetuses were separated from their mother for further preparation and analysis. Thedata were analyzed statistically by using SPSS 22 version. If they were distributed normally, the data would beanalyzed usingOneway ANOVA and Duncan Multiple Range Test (DMRT) to see the difference among the groups.Diets containing Calliandra leaf flour fed on pregnant rats decreased weight and length of fetuses and increasedfetus hemorrhage. However, it did not cause dead fetus or decreased the number of life fetuses. Diets containingCalliandra leaf flourfed to pregnant rats did not affect the ossification of vertebrae, costae and sternebrae. However,it inhibited ossification of metacarpus, metatarsus and vertebrae caudalis.
Prevalence and gene frequency of colour blindness among students of elementary schools in Badung Regency, Bali, Indonesia Ni Wayan Karolina; Made Pharmawati; Iriani Setyawati
Jurnal Biologi Udayana Vol 23 No 2 (2019): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.742 KB) | DOI: 10.24843/JBIOUNUD.2019.v23.i02.p01

Abstract

Buta warna merupakan penyakit keturunan yang terpaut kromosom-X. Buta warna terdiri atas tiga tipe yaitumonokromat (buta warna total), dikromat (buta warna parsial), dan anomali trikromat. Sebagian besar cacatbuta warna tidak terdeteksi, sehingga perlu dilakukan identifikasi sejak dini. Tujuan dari penelitian iniadalah untuk mengetahui prevalensi dan frekuensi gen buta warna pada populasi siswa SD di KabupatenBadung. Kabupaten Badung merupakan salah satu kabupaten di Bali yang terdiri atas enam kecamatan yaituKecamatan Petang, Kecamatan Abiansemal, Kecamatan Mengwi, Kecamatan Kuta Utara, Kecamatan Kuta,dan Kecamatan Kuta Selatan. Penelitian dilakukan di 11 Sekolah Dasar di tiga kecamatan (Petang,Abiansemal, dan Kuta Utara) dengan total probandus 900 orang. Pengujian cacat buta warna dilakukandengan uji Ishihara plate test, kemudian data dianalisis dengan SPSS versi 22. Frekuensi gen dikalkulasidengan metode Hardy-Weinberg equilibrium. Prevalensi buta warna pada siswa SD di Kabupaten Badungyaitu 2% dari 900 probandus. Frekuensi gen buta warna pada laki-laki yaitu 0,0378 dan pada perempuanyaitu 0,0022. Perbedaan jenis kelamin pada frekuensi buta warna didapatkan signifikan secara statistik (P <0,0001), dengan prevalensi pada laki-laki 3,78% lebih tinggi dibandingkan perempuan 0,22%
MORFOLOGI FETUS MENCIT (MUS MUSCULUS L.) SETELAH PEMBERIAN EKSTRAK DAUN SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) IRIANI SETYAWATI
Jurnal Biologi Udayana Vol 13 No 2 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This experiment was performed to examine the effects of Andrographis paniculata Nees leaves extract on foetus morphology if given during organogenesis period. Thirty pregnant mice were randomly divided into 5 groups for teratogenic effects. Extract was given orally by gavage with 0 (as control); 0,004 (equal to one time dose to human); 7,5; 15; and 22,5 g/g body weight/ day. Treatment was given from day 6 to day 13 of gestation (organogenesis period). Foetus were removed on day 18 of gestation by caesarean section. Quantitative data were analyzed with Anova and Duncan's Multiple Range Test. The results showed that teratogenic effects increased in dose-related manner by causing foetus death and resorption, hemorrhage and clubfoot.
PERTUMBUHAN, HISTOPATOLOGI OVARIUM DAN FEKUNDITAS IKAN NILA MERAH (OREOCHROMIS NILOTICUS) SETELAH PAPARAN PESTISIDA ORGANOFOSFAT IRIANI SETYAWATI; NGURAH INTAN WIRATMINI; JOKO WIRYATNO
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Fish cultures in rice fields or areas that use water from a pesticide-polluted irrigation or river, are particularly vulnerable to the negative impact of pesticides. This study aims to assess the effect of organophosphates on growth, ovarian histopathology and fecundity of red tilapia (Oreochromis niloticus). After acclimatization, 160 fishes (fingerlink stage) were kept in four ponds sized 2x1,5x0,8 m each. This study used a complete randomized block design and organophosphate administration through the feed. The study used four groups: control, and variations in dose of 2 ml (D1), 6 ml (D2), and 10 ml (D3) of pesticide per 100 grams of pellet, which is given in the morning (treatment) and in the evening (regular feed). Data were taken at week 3, 6, and 9, then the organophosphate treatments were stopped, and the last data were taken at week-18. Statistical analysis was performed by Anova and Duncan's Multiple Range Test. The results showed that the organophosphate pesticide inhibits the growth by reducing fish length and weight, reducing ovarian weight, increasing the number of oocyte atresia, damaging structure of the ovarian histology, and decreasing fecundity by reducing the number of egg production in the ovarian of red tilapia.
VARIASI BENTUK WAJAH LAKI-LAKI DAN PEREMPUAN DARI DESA PANGLIPURAN, KABUPATEN BANGLI, PROVINSI BALI AGUNG EKO CAHYA KUSUMA PUTRA; NI NYOMAN WIRASITI; IRIANI SETYAWATI
Jurnal Biologi Udayana Vol 21 No 2 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.277 KB) | DOI: 10.24843/JBIOUNUD.2017.vol21.i02.p03

Abstract

This research determined male and female face type of the adults in Panglipuran adults. The analysis of face types Geometric Morphometric Technique in thin-plate-splin (tps) program. Adults female and 14 male aged 25-35 years old were photographed for both frontal and lateral faces with a digital camera Canon EOS 60D. Landmark data was processed by photo processor program tpsDig version 2.04, tpsSuper version 1.13, tpsRelw version 1.42, tpsSplin 1.20 and both of ape and gee package of R Program version 3.2.5. The average of male’s face shape type in Panglipuran village was ellipse and round for female’s. We found 2 frontal and 2 lateral for male’s face variation, we also found 3 frontal and 3 lateral for female’s. Frontal face of male and female that showed high variation included right subaurale, left subaurale, right gonion, left gonion, and gnathion. Lateral face of male and female that showed high variation were trichion, frontozygomaticus, superciliare, superaurale, pogonion, glabella, the minimum point of the curve below earlobe, gonion and gnathion.
Co-Authors Adevalentin Lesik AGUNG EKO CAHYA KUSUMA PUTRA Agustina, Tika Puri Alfonds Andrew Maramis Anak Agung Ketut Darmadi Andayani, Ni Kadek Prederika Sari Areningrat, Putu Ayutia Daud, Putri Deny Suhermawan Yusup Dwi Ariani Yulihastuti Ezra Oktavia Ambarita Fernando Andre Watung Fita Efi Rafelina Siallagan Futwembun, Fransiska FX. Sudaryanto Graciella Stevani Gulo Haryo Seto Wicaksono Hasmiati Hasmiati Helen J Lawalata Helen Joan Lawalata Hety Hartaty I Gede Agus Pradana Putra I Gede Wiranatha I Gusti Agung Ayu Suartini I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Gusti Ngurah Agung Dewantara Putra I Ketut Junitha I Made Sara Wijana I Wayan Wena Diartha Ida Ayu Ratih Purnama Dewi Ida Bagus Made Suaskara Inna Narayani Jaqueline E.M Tangkau Joko Wiryatno Kadek Ayu Trisna Yanti Kadek Mardika Kadek Mardika Kamagi, Decky LUH Puru ESWARYANTI KUSUMA YUNI Luh Putu Eswaryanti Kusuma Yuni MADE PHARMAWATI Martin Joni Mokosuli Yermia Samuel Musma Rukmana Ngurah Intan Wiratmini Ni Gusti Ketut Roni Ni Kadek Prederika Sari Andayani Ni Komang Frely Astuti Ni Made Estriana Pranadya Ni Made Suartini Ni Nyoman Wirasiti Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Wayan Karolina Ni Wayan Sudatri Nonny Manampiring Nova L.I.M Ogi Nur Assiam Orbanus Naharia Retno Kawuri Revolson Mege Rolef Rumondor Rompas, Christny Ferdina Evie Ruth Ellisa Christiani Sambeka, Delvino SANG KETUT SUDIRGA Supit, Annely Suranjaya I .Gd Verawati I Y Roring Wiesye Nangoy Yohana Deni Suryati