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IRIANI SETYAWATI
Biology Study Program, Faculty Of Mathematics, Natural And Earth Sciences, Universitas Negeri Manado, Tondano Campus, North Sulawesi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Identification of Bacteria Isolated from Sea Urchin (Diadema setosum) Gonads Inhibiting Pseudomonas fluorescens and Staphylococcus aureus Growth Andini Maurent Dwiyanti; Helen Joan Lawalata; Iriani Setyawati; Anita Constanci Christine Tengker; Nonny Manampiring
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 2 (2025): June 2025
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i02.p05

Abstract

Sea urchins are organisms that have antibacterial, antitumor, and anticancer abilities. The gonads can be used as a food source because they store 28 kinds of amino acids, vitamin B complex. This research aims to determine the type of bacteria from the gonads of sea urchins (Diadema setosum) which have inhibitory power against the growth of Pseudomonas fluorescens and Staphylococcus aureus bacteria. This research used an exploratory method which was analyzed descriptively, using stages in which sea urchin samples were taken and antibacterial tests against Pseudomonas fluorescens and Staphylococcus aureus from sea urchin gonads using the spread method. The media used for the process of isolating bacteria from the gonads of the sea urchin Diadema setosum is Nutrient Agar (NA) media. Isolate colonies that grew after incubation for 24 hours were separated based on differences in colony morphology. Morphological characterization of bacterial cells begins with gram staining. The diameter of the inhibition zone in the test bacteria shows different shapes. The research obtained 8 bacterial isolates, namely isolates B1-B8 which were gram-negative. The eight bacterial isolates have the potential as antibacterials against the test bacteria P. fluorescens and S. aureus. Isolates B1, B2, B3, B7 are similar to the genus Proteus, while isolates B4, B5, B6, and B8 are similar to the genus Citrobacter. The diameter of the largest inhibition zone in the P.fluorescens test bacteria, namely isolate B1, was 9.03 mm. Meanwhile, the smallest diameter for isolate B7 was 6.97 mm. In the S.aureus test bacteria, the largest diameter in isolate B1 was 11.09 mm. Meanwhile, the smallest diameter for isolate B4 was 8.57 mm.
Efektivitas Serbuk Daun Kersen (Muntingia calabura L.) dan Daun Kelor (Moringa oleifera) terhadap Kadar Glukosa Darah Tikus Putih (Rattus norvegicus L.) Hiperglikemia Elsi Elsi; Iriani Setyawati; Nonny Manampiring; Yermia Semuel Mokosuli; Anita Constanci Christine Tengker; Helen Joan Lawalata
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p6

Abstract

Dua puluh tujuh ekor tikus putih (Rattus norvegicus L.) jantan dibagi menjadi 9 kelompok perlakuan yaitu kontrol normal (K1), kontrol negatif (K2), kontrol positif (K3), serbuk daun kersen 800 mg/kgB (K4), serbuk daun kelor 800 mg/kgBB (K5), serbuk daun kersen 1600 mg/kg BB (K6), serbuk daun kelor 1600 mg/kgBB (K7), kombinasi serbuk daun kersen 400 mg/kgBB dan daun kelor 400 mg/kg BB (K8), kombinasi serbuk daun kersen 800 mg/kg BB dan daun kelor 800 mg/kgBB (K9). Pengukuran kadar glukosa darah tikus dilakukan pada hari ke-3, 7, dan 10. Hasil analisis menunjukan bahwa pemberian dosis kombinasi serbuk daun kersen 800 mg/kgBB dan daun kelor 800 mg/kgBB merupakan dosis yang paling efektif dalam menurunkan kadar glukosa darah pada tikus putih jantan
EFFECT OF GEL FORMULATION CONTAINING Tridax procumbens AND Aloe vera LEAF EXTRACT ON THE HEALING OF SECOND-DEGREE BURNS IN MICE (Mus musculus) Maturbongs, Marsela; Setyawati, Iriani; Manampiring, Nonny; Lawalata, Helen; Taulu, Marthy; Rompas, Christny
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/n2vkg912

Abstract

Tridax procumbens L. and Aloe vera L. are plants commonly used in the treatment of burns. This study was conducted to determine the effect of giving a combination gel of T. procumbens and A. vera leaf extract on second-degree burn healing in mice. This study used 24 male mice (Mus musculus) which were divided into four treatment groups, namely positive control (KP, Bioplacenton®); negative control (KN, gel base); leaf extract of T. procumbens 0.5% and A. vera 0.5% (P1); and leaf extract of T. procumbens 1% and A. vera 0.5% (P2). The process of inducing burns on the backs of mice requires the use of a 1x1 cm iron, which is subjected to heat in a fire for three minutes. The iron is placed on the mice's back for five seconds, which leads to the development of a second-degree burn. The gel was applied twice a day for 21 days. The highest level of burn wound healing was recorded with the Bioplacenton® treatment, which achieved a full recovery of 100% on day 15. Following this, the combined gel treatment of 1% T. procumbens leaf extract and 0.5% A. vera also reached 100% healing by day 18. While it may not be as potent as Bioplacenton®, the gel formulation containing 1% T. procumbens and 0.5% A. vera leaf extract presents a viable alternative for treating second-degree burns.
CHARACTERISTICS OF TURTLE NESTING HABITAT ON POPARENG BEACH, TATAPAAN DISTRICT, SOUTH MINAHASA REGENCY, BUNAKEN NATIONAL PARK AREA Mukuan, Randi; Lawalata, Helen; Tengker, Anita; Setyawati, Iriani; Mege, Revolson Alexius
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/mbaj0069

Abstract

Turtles They are among the world's protected animals and one of the rare, almost extinct species. The preservation of their habitat and knowledge of the traits of turtle-friendly habitats are two ways that the rescue and protection of these turtles must go on. For rescue operations, turtle sustainability, and sustainable coastal management, research is being conducted on the features of the turtle nesting habitats on Popareng Beach. This research aims to determine the characteristics of turtle nesting habitats and identify the parameters that hinder turtles from nesting at Popareng Beach, Tatapaan District, South Minahasa Regency. The method used in this research is the descriptive-exploratory method. With characteristic parameters consisting of beach width measurement, beach slope, sand temperature, sand humidity, sand texture, beach vegetation, and the presence of predators. From the research results, it shows that station 1 and station 2 have results that are suitable for turtle nesting habitats, with values obtained at station 1 being 88.33% and station 2 being 92%. Meanwhile, at station 3, with an evaluation score of 72%, it is considered fairly suitable and the station has tolerable limitations.
EFEKTIVITAS SEDIAAN GEL EKSTRAK DAUN PACAR AIR DAN LIDAH BUAYA TERHADAP PENYEMBUHAN LUKA SAYAT MENCIT Gabriela Tasya Paat; Iriani Setyawati; Meity Neltje Tanor; Helen Joan Lawalata; Anita Constanci Christine Tengker; Nonny Manampiring
Edumedia : Jurnal Keguruan dan Ilmu Pendidikan Vol 10 No 1 (2026): Edumedia: Jurnal Keguruan dan Ilmu Pendidikan
Publisher : Universitas Kapuas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51826/edumedia.v10i2.1906

Abstract

Penelitian ini bertujuan untuk mengetahui efektivitas kombinasi ekstrak daun pacar air (Impatiens balsamina, L.) dan lidah buaya (Aloe vera) dalam proses penyembuhan luka sayatan. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. Hewan uji yang digunakan adalah mencit (Mus musculus) jantan dewasa yang dibagi dalam 5 kelompok, yaitu kontrol positif (F0, bioplacenton); kontrol negatif (F1, tanpa perlakuan); perlakuan formula sediaan gel dari ekstrak daun pacar air 2,5% dan lidah buaya 2,5% (F2); pacar air 5% dan lidah buaya 5% (F3); pacar air 10% dan lidah buaya 10% (F4). Bagian paha belakang kanan mencit yang sebelumnya telah dicukur dan diolesi alkohol 70% dibuat luka sayat dengan silet sepanjang 10 mm dan kedalaman 2 mm. Pemberian sediaan gel dilakukan setiap hari pada jam 10.00 WITA selama 12 hari. Hasil penyembuhan luka sayat yang paling tinggi ditunjukkan oleh kelompok F4 dan F3, di mana pada hari ke-8 luka sudah menutup sempurna. Hasil penelitian menunjukkan bahwa pemberian sediaan gel ekstrak daun tumbuhan pacar air 5% dan lidah buaya 5% lebih efektif dalam penyembuhan luka sayat daripada Bioplacenton.
Co-Authors Adevalentin Lesik AGUNG EKO CAHYA KUSUMA PUTRA Agustina, Tika Puri Alfonds Andrew Maramis Anak Agung Ketut Darmadi Andayani, Ni Kadek Prederika Sari Andini Maurent Dwiyanti Anita Constanci Christine Tengker Anita Constanci Christine Tengker Anita Costanci Christine Tengker Areningrat, Putu Ayutia Arrijani - Christny Ferdina Evie Rompas Daud, Putri Decky D. W. Kamagi Deny Suhermawan Yusup Dino Rahardiyan Dwi Ariani Yulihastuti Elsi Elsi Emma M Moko Esrah Anna Adeleid Agimat Ezra Oktavia Ambarita Feby Febrianty Toding Rante Fernando Andre Watung Fita Efi Rafelina Siallagan Futwembun, Fransiska FX. Sudaryanto Gabriela Tasya Paat Graciella Stevani Gulo Haryo Seto Wicaksono Hasmiati Hasmiati Helen Joan Lawalata Helen Joan Lawalata Hety Hartaty I Gede Agus Pradana Putra I Gede Wiranatha I Gusti Agung Ayu Suartini I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Gusti Ngurah Agung Dewantara Putra I Ketut Junitha I Made Sara Wijana I Wayan Wena Diartha Ida Ayu Ratih Purnama Dewi Ida Bagus Made Suaskara Inna Narayani Jaqueline E.M Tangkau Joko Wiryatno Kadek Ayu Trisna Yanti Kadek Mardika Kadek Mardika Lawalata, Helen Livana Dethris Rawung LUH Puru ESWARYANTI KUSUMA YUNI Luh Putu Eswaryanti Kusuma Yuni MADE PHARMAWATI Manampiring, Nonny Marthy L S Taulu Martin Joni Maturbongs, Marsela Megawati Meylin Weken Meity Neltje Tanor Mokosuli Yermia Samuel Mukuan, Randi Musma Rukmana Nangoy, Wiesye M. S> Ngurah Intan Wiratmini Ni Gusti Ketut Roni Ni Kadek Prederika Sari Andayani Ni Komang Frely Astuti Ni Made Estriana Pranadya Ni Made Rai Suarni Ni Made Suartini Ni Nyoman Wirasiti Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Wayan Karolina Ni Wayan Sudatri Nonny Manampiring Nova L.I.M Ogi Nur Assiam Orbanus Naharia Orbanus Naharia Retno Kawuri Revolson Mege Rievo Handry Djarang Rolef Rumondor Rompas, Christny Rosenta Sipayung Ruth Ellisa Christiani Sambeka, Delvino SANG KETUT SUDIRGA Supit, Annely Suranjaya I .Gd Verawati I Y Roring Wiesye Nangoy Yohana Deni Suryati