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Cover, Editors, and Table of Content of ATBES Vol.1 No.2 Sept 2017 Iriani Setyawati
Advances in Tropical Biodiversity and Environmental Sciences Vol 1 No 2 (2017): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.397 KB)

Abstract

Advances in Tropical Biodiversity and Environmental Sciences (ATBES) is a scientific journal published since 2017. The journal consistently publishes twice a year (Pebruary and September). The journal is registered in DOAJ. ATBES is employing a Cross Check Ref. to prevent any suspected plagiarism in the manuscripts.
Lung Histopathology of Laying Hens Infected by Colibacillosis in the Animal Cages Experiments of the Disease Investigation Center 6, Denpasar, Bali Kadek Ayu Trisna Yanti; Iriani Setyawati; Ni Putu Adriani Astiti
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 2 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1217.111 KB) | DOI: 10.24843/ATBES.2019.v03.i02.p02

Abstract

This study aimed to determine the lungs histopathology of laying hens (Gallus gallus domesticus) at the Animal Cage Experiments in the Disease Investigation Center 6, Directorate General of Live Stock (DIC-6 DGLS), Denpasar, Bali, which died from colibacillosis infection. Sample of lungs were cut transversely then put into 10% of Neutral Buffer Formalin, then processed histologically by paraffin method and stained with Hematoxylin-Eosin. Observation under microscope (magnification 100x and 400x) was done for histopathological examination. Laying hens died from colibacillosis infection showed that their lungs were infected by colibacillosis, and there were found 62.50% of necrosis, 75% of inflammatory cells infiltration and 80% of hemorrhage in the lungs.
Histology of Rat Pancreas Treated with Calliandra calothyrsus Leaf in the Diets during Pregnancy and Lactation Iriani Setyawati
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 1 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.621 KB) | DOI: 10.24843/ATBES.2019.v03.i01.p01

Abstract

For cattle, red calliandra (Calliandra calothyrsus) is a good protein source of forage but the condensed tannin content is the highest compared to other legume plants. Tannin can reduce the activity of digestive enzymes and protein digestibility, which can lead to pancreatic hypertrophy and hyperplasia. This study aimed to determine the pancreatic histology of pregnant rats treated with C.calothyrsus leaf flour during pregnancy and lactation period. This study used a completely randomized design with 24 pregnant female rats which were divided into four groups i.e. control and treatments of 10, 17.5 and 25% of C.calothyrsus leaf flour in the diets. The treatment started from the first day of pregnancy until the young rats were weaned at the age of 21 days. Pancreatic histological structure of the mother showed an increase lack of normal tissue density and an increase connective tissues in the pancreas descriptively, especially at the highest level of C.calothyrsus leaf flour (25%) in the diets.
MORPHOMETRY OF EAGLE SPECIES (FAMILY ACCIPITRIDAE) AT BALI BIRD PARK Iriani Setyawati; L.P. Eswaryanti Kusuma Yuni; Retno Kawuri; Ni Made Suartini; Ni Wayan Sudatri; I Gede Agus Pradana Putra
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

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Abstract

This study measured the morphometry of raptor species (Family Accipitridae) in Bali Bird Park (BBP) namely Nisaetus cirrhatus (Changeable Hawk Eagle), Elanus caeruleus (Black Winged Kite), Spilornis cheela (Crested Serpent Eagle) and Haliaeetus leucogaster (White Bellied Sea Eagle). Measurements were made with meter tape and caliper on birds that were in their resting period. Raptor at BBP generally rest for 6 months until experience molting before being retrained for 4-5 months, then the birds will be ready to perform bird show for the next 9-12 months. Our measurements obtained the largest bird was Haliaeetus leucogaster with a total body length of 75 cm, wings length 83.5-85 cm, tail length 26 cm, head size (8 cm length and 7 cm width), upper beak (8 cm length and 2.5 cm thick) and lower beak (3 cm length and 1 cm thick), lower limb length (femur 17 cm, tibia 9 cm, metatarsus 11 cm, middle toe 6.5 cm and grip 9 cm). The smallest bird was Elanus caeruleus with a total body length of 33 cm, wing length 39-45 cm, tail length 19 cm, head size (8 cm length, 5.5 cm width), upper beak (2.5 cm length and 0.5 cm thick) and lower beak (1 cm length and 0.3 cm thick), lower limb length (femur 8 cm, tibia 4 cm, metatarsus 5 cm, middle toe 3 cm and grip 5 cm) The morphometry of the species Nisaetus cirrhatus and Spilornis cheela were between the two other bird species.
Blood Glucose Level and Pancreas Histological Section of Male Rabbits after Being Fed Commercial Feed Supplemented with Cod Liver Oil Ni Gusti Ayu Manik Ermayanti; Ida Bagus Made Suaskara; I Gusti Ayu Manik Widhyastini; Iriani Setyawati
JURNAL SAINS NATURAL Vol. 13 No. 1 (2023): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v13i1.441

Abstract

Fish oil contains eicosapentaenoic acid (E.P.A.) and docosahexaenoic acid (D.H.A.) which are beneficial for heart health, controlling blood glucose, reducing atherosclerosis, and lowering the risk of cancer. This study aims to determine the effect of commercial feed supplemented with cod liver oil on blood glucose levels and pancreatic histology of male rabbits. Twenty rabbits were divided into four groups, namely commercial feed supplemented with cod liver oil 3% (P1), 4.5% (P2), 6% (P3), and commercial feed without cod liver oil supplementation (control). Each group consisted of five rabbits, and the treatment was given for four weeks. The parameters observed were blood glucose levels in the 1st, 2nd, 3rd, 4th, and 5th weeks and the histology of the male rabbits’ pancreas. Data were analyzed by Anova. The results showed that in the 1st, 2nd, and 3rd weeks there was no significant difference (P>0.05). In contrast, in the 4th  and 5th weeks, there was a significant difference (P<0.05) between the control and treatment of cod liver oil supplementation on blood glucose levels in male rabbits, and the observational results on the histology of rabbits showed normal pancreatic histology. In conclusion, cod liver oil supplementation on commercial feed controlled blood glucose levels, and there was no disturbance in the histological structure of the pancreas of male rabbits.Keywords: fatty acid, glucose, cod liver oil, pancreas, rabbit
Analisis Aspek Morfometri Dan Biologi Reproduksi Tongkol Lisong (Auxis rochei) yang Didaratkan Di Ppi Kedonganan, Bali Yohana Deni Suryati; Iriani Setyawati; Deny Suhernawan Yusup
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p04

Abstract

Objective of this research is to study morphometry and reproduction biology of bullet tuna (Auxis rochei, Risso 1810), landed in PPI (Pangkalan Pendaratan Ikan – Fish Landing Base) Kedonganan, Bali. Morphometric aspect covers distribution of length measurement and correlation of length-weight, while gonad maturity is observed hystologically based on gonad cell growth. Result of this study shows that the length of bullet tuna ranges from 16.5 to 35.3 cmFL, with weight extends from 64.8 to 539.4 gram. Growth pattern of bullet tuna is positively allometric (b>3). Generally, landed bullet tuna in PPI Kedonganan are dominated by mature fish with Gonad Maturity Level III, IV, and V by as many as 123 individuals (58.85%) within 22 – 23 cmFL length. The difference of gonad maturity result that bullet tuna spawning season is asynchronous (multiple spawning) and partial spawning Keywords : bullet tuna, length – weight correlation, reproduction aspect, gonad
Potential of Calliandra calothyrsus Leaf Extract to Maintain Estrogen Concentration and Uterine Thickness in Rats Iriani Setyawati; Ni Nyoman Wirasiti; Luh Putu Eswaryanti Kusuma Yuni
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.31063

Abstract

Calliandra calothyrsus Meissn. leaf extract is potential as phytoestrogens. It influenced male mice reproduction, rat estrous cycle, and ovarian histology in previous study. This research aimed to prove the C. calothyrsus leaf extract potential as phytoestrogen source and the effect on endometrial thickness where the embryo implantation take place in early pregnancy. This study used a Completely Randomized Design used 54 days old female rats (Rattus norvegicus). Rats were divided into K as control group (treated with 0.5% Na-CMC as placebo) and P1, P2, and P3 as three groups with C. calothyrsus leaf extract administration with doses of 17.5; 35; and 70 mg/ kg bw respectively. Treatments were given 1 ml/rat/day orally for 20 days. At day 21st, animals were euthanized to collect blood samples for estrogen hormone analysis. After the dissection, all uterus were collected and weighed. Histological preparation was done with paraffin method and Hematoxylin-Eosin staining. The effective dose was 70 mg/ kg bw that did not decrease the weight of the uterus and the body. This dose even maintained the normal diameter and thickness of uterine walls (endometrium, myometrium, and perimetrium layers) like control rats. The extract in this study could increase estrogen concentration in female rats. This research novelty is that C. calothyrsus leaf extract (70 mg/ kg bw) can be used as an alternative herbal suplement to maintain uterine wall thickness and estrogen concentration in productive women. With further clinical research, this extract is a good candidate as potential estrogen source to overcome women infertility or pregnancy difficulties due to problem of endometrial thickness and lack of endogenous estrogen.
MORPHOMETRY OF EAGLE SPECIES (FAMILY ACCIPITRIDAE) AT BALI BIRD PARK Iriani Setyawati; L.P. Eswaryanti Kusuma Yuni; Retno Kawuri; Ni Made Suartini; Ni Wayan Sudatri; I Gede Agus Pradana Putra
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v3i3.5733

Abstract

This study measured the morphometry of raptor species (Family Accipitridae) in Bali Bird Park (BBP) namely Nisaetus cirrhatus (Changeable Hawk Eagle), Elanus caeruleus (Black Winged Kite), Spilornis cheela (Crested Serpent Eagle) and Haliaeetus leucogaster (White Bellied Sea Eagle). Measurements were made with meter tape and caliper on birds that were in their resting period. Raptor at BBP generally rest for 6 months until experience molting before being retrained for 4-5 months, then the birds will be ready to perform bird show for the next 9-12 months. Our measurements obtained the largest bird was Haliaeetus leucogaster with a total body length of 75 cm, wings length 83.5-85 cm, tail length 26 cm, head size (8 cm length and 7 cm width), upper beak (8 cm length and 2.5 cm thick) and lower beak (3 cm length and 1 cm thick), lower limb length (femur 17 cm, tibia 9 cm, metatarsus 11 cm, middle toe 6.5 cm and grip 9 cm). The smallest bird was Elanus caeruleus with a total body length of 33 cm, wing length 39-45 cm, tail length 19 cm, head size (8 cm length, 5.5 cm width), upper beak (2.5 cm length and 0.5 cm thick) and lower beak (1 cm length and 0.3 cm thick), lower limb length (femur 8 cm, tibia 4 cm, metatarsus 5 cm, middle toe 3 cm and grip 5 cm) The morphometry of the species Nisaetus cirrhatus and Spilornis cheela were between the two other bird species.
Estrous cycle and uterine weight of ovariectomized menopause female rat models after treatment with Leucaena leucocephala (Lam.) de Wit leaf extract Ngurah Intan Wiratmini; Anak Agung Sagung Alit Sukmaningsih; Iriani Setyawati
Folia Medica Indonesiana Vol. 60 No. 2 (2024): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v60i2.53740

Abstract

Highlights:1. This study found that Leucaena leucocephala leaf ethanol extract has the ability to stimulate the growth of vaginal epithelial cells in ovariectomised rats.2. Leucaena leucocephala leaf extract can be developed as a safe and cost-effective natural alternative for hormone replacement therapy in premenopausal women and livestock.   Highlights:1. This study found that Leucaena leucocephala leaf ethanol extract has the ability to stimulate the growth of vaginal epithelial cells in ovariectomised rats.2. Leucaena leucocephala leaf extract can be developed as a safe and cost-effective natural alternative for hormone replacement therapy in premenopausal women and livestock.   Abstract Hormone replacement therapy has adverse effects that may cause additional health problems, such as hot flashes, cancer, ischemic stroke, and death. Phytoestrogen is an alternative ingredient used for hormone replacement therapy with less risk. The Leucaena leucocephala (Lam.) de Wit plant is widely used as a medicinal ingredient and animal feed. This study aimed to determine the effect of Leucaena leucocephala leaf extract on the uterine weight and estrous cycle of ovariectomized rats. This research used a completely randomized control design, which included two treatment groups (P1 and P2) and two control groups (negative (K-) and positive (K+)) with six replications in each group (n=24). The negative control (K-) was given 2 mL of corn oil, while K+ received 0.1 mg/kg bw of 17β-estradiol. P1 and P2 were administered 250 and 300 mg/kg bw of Leucaena leucocephala leaf extract, respectively. The extract was administered daily by gavage for 30 days. Estrous cycle data were obtained on day 31 by previously collecting vaginal swabs twice a day for 15 days. After the rats were sacrificed under anesthesia using chloroform, they underwent surgery to remove the uterine organs for weight measurement. The data were analyzed for normality and homogeneity using the Shapiro-Wilk and Levene tests. The data distribution were not normal, so the Kruskal Wallis test was used to test the hypothesis. If there was a difference between controls and treatments, the analysis continued with the Mann-Whitney test (p<0.05). The controls and treatments exhibited a significant difference in the length of each phase and overall estrous cycle. The post hoc Duncan test revealed that the highest uterine weight was found in 17β-estradiol treated rat. It was significantly different with both extracts treated rats. Both extract effect on uterine weight were not significantly difference. In conclusion, administering Leucaena leucocephala leaf extract results in an increased uterine weight and a normal estrous cycle in ovariectomized rats.
Chronic Toxicity Effect of Mimosa pudica Leaf Extract Towards Histology Profile of Stomach and Duodenum in Mice Andayani, Ni Kadek Prederika Sari; Setyawati, Iriani; Sudatri, Ni Wayan
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 2 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n2.p52-59

Abstract

In Indonesia, Mimosa pudica Linn. (M. pudica L.) is a wild plant (weed) from the Mimosaceace family. This plant is widely used as a traditional medicine for various types of diseases such as insomnia, acute eye inflammation, urolithiasis (urinary stones), fever, and bronchitis. This study aimed to find out the effect of M. pudica leaf extract on the histological damage of the stomach and duodenum of mice. This study used a Completely Randomized Design with 24 male mice, which were divided into control placebo (P0) given CMC-Na 0.5%; and M. pudica leaf extract doses of 200 mg/kg body weight (P1); 400 mg/kg body weight (P2); and 600 mg/kg body weight (P3). The extract was administered orally for 28 days. On day 29, the animal was dissected to collect its digestive organs. The histological preparation of the stomach and duodenum of mice was performed by using the paraffin method and Hematoxylin and Eosin staining to observe the histological damage, namely congestion, hemorrhage, epithelial cells desquamation, inflammatory cell infiltration, edema, and hyperplasia. Quantitative data were analyzed with a one-way ANOVA test and Duncan's post hoc test with the SPSS version 22. The results showed that the M. pudica leaf extract increased hemorrhage and hyperplasia damage at doses of 400 and 600 mg/kg body weight in mice’s duodenum.
Co-Authors Adevalentin Lesik AGUNG EKO CAHYA KUSUMA PUTRA Agustina, Tika Puri Alfonds Andrew Maramis Anak Agung Ketut Darmadi Andayani, Ni Kadek Prederika Sari Areningrat, Putu Ayutia Daud, Putri Deny Suhermawan Yusup Dwi Ariani Yulihastuti Ezra Oktavia Ambarita Fernando Andre Watung Fita Efi Rafelina Siallagan Futwembun, Fransiska FX. Sudaryanto Graciella Stevani Gulo Haryo Seto Wicaksono Hasmiati Hasmiati Helen J Lawalata Helen Joan Lawalata Hety Hartaty I Gede Agus Pradana Putra I Gede Wiranatha I Gusti Agung Ayu Suartini I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Gusti Ngurah Agung Dewantara Putra I Ketut Junitha I Made Sara Wijana I Wayan Wena Diartha Ida Ayu Ratih Purnama Dewi Ida Bagus Made Suaskara Inna Narayani Jaqueline E.M Tangkau Joko Wiryatno Kadek Ayu Trisna Yanti Kadek Mardika Kadek Mardika Kamagi, Decky LUH Puru ESWARYANTI KUSUMA YUNI Luh Putu Eswaryanti Kusuma Yuni MADE PHARMAWATI Martin Joni Mokosuli Yermia Samuel Musma Rukmana Ngurah Intan Wiratmini Ni Gusti Ketut Roni Ni Kadek Prederika Sari Andayani Ni Komang Frely Astuti Ni Made Estriana Pranadya Ni Made Suartini Ni Nyoman Wirasiti Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Wayan Karolina Ni Wayan Sudatri Nonny Manampiring Nova L.I.M Ogi Nur Assiam Orbanus Naharia Retno Kawuri Revolson Mege Rolef Rumondor Rompas, Christny Ferdina Evie Ruth Ellisa Christiani Sambeka, Delvino SANG KETUT SUDIRGA Supit, Annely Suranjaya I .Gd Verawati I Y Roring Wiesye Nangoy Yohana Deni Suryati