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Journal : Jurnal Veteriner

THE DEVELOPMENT OF FOLLICLES AND OOCYTES VIABILITY FROM EWE OVARIUM POST-INTRAUTERINE TRANSPLANTATION TO PSEUDOPREGNANT RABBIT Ramadhan Sumarmin; Adi Winarto; Tutty Laswardi Yusuf; Arief Boediono
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to evaluate the development of ewe follicles and oocytes viabilityfollowing an intrauterine transplantation of the ovaries into pseudopregnant rabbit. The experiment wasconducted in day 1 of pseudopregnant rabbit. Following transplantation for 5, 7, and 9 days, the ovarieswere recollected. The development of follicles determined by counting the number of follicles in paraffinembedded ovaries after staining with haematoxylin-eosin (HE). The viability of oocytes was determined byslicing the ovaries. Oocytes were incubated in CO2 incubator with 5% CO2, 38°C for 24 hours. After maturation,the oocytes were stained with 2% aceto-orcein to determine the nuclear oocytes status. The result showedthat follicles were detected in all stages of their development (primordial, primary, preantral, and antralfollicle stages), but their number decreased significantly (P<0.05) 5, 7 or 9 days after transplantation,except for those at primordial stage which at day 5 post-transplantation (634.7±56.88) were not significantlydifferent to the control (683.7±61.55). After maturation, the oocytes that were able to reach the M-II phaseat day 5 and day 7 post-transplantation were 35.05% and 35.24% respectively. They were significantly(P<0.05) lower than the control (56.65%). In conclusion, the development of follicles and oocytes viability inthe ewe ovaries in pseudopregnant rabbits was still preserved during intrauterine transplantation.
Co-Authors A.A. Ketut Agung Cahyawan W Abdul Rajak Abdul Razak Abdul Razak Adi Winarto Afriani Afriani Ahyar, Rania Annika Maizeli Arafah, Auliani Ardi Ardi Arianti, Febri Arief Boediono Armein Lusi Ze swita Armein Lusi Zeswita Armen Lusi Asrizal Azwar Ananda Azwir Anhar Damaiyanti, Riska Defrian Melta Delni Oktavia Deni eliza Desi Anggriani Dewinda Yanua Putri Diana Susanti Diana Susanti, Febriyanti, Ruth Rize Paas Megahati S, Diana Susanti, Dra. NURSYAHRA M.Si Dra. NURSYAHRA M.Si Dwi Hilda Putri Dwi Nugraha, Fitra Arya Effendi, Jon Elda Riza Elsa Yuniarti Ennike Gusti Rahmi Ernie Novriyanti F, Farida Festiyed Fetro Dola Samsu Fingki Alvia Candra Fitri Arsih Fitri Malinda Sari Fitri, Ryantika Fitria Dona gusril gusril Gustina Indriati Hayuni Octavia Heffi Alberida Helendra Helendra Huryah, Fadhilatul Indra Madani Irawati, Wike Julia Maria Gorette Juni Asmi Lumban Toruan Kurniasih, Yulvina Lauren Lauren Linda Advinda Liza Yulia Sari Lufri Lufri M. Haviz M. Yogi Riyantama Isjoni Mailastri Suarna Margaretta Margaretta Maria Fransiska Mawarni Mawarni Meliya Wati Miswati Miswati Muhyiatul Fadilah Muthia Oktavia Natalia, Desfaur Nia Anggraini Nila Kusuma Wati Nindi Maiyora Nofri Zayani Nurul Fauziah Pardismi Pardismi Penatriwati Penatriwati Putra, Erik Nanda Putri, Rani Dwi Suci Rahma Wati Rahmadhani Fitri Rahmah Maulidiyah Rahmapela Zaima Rahmawati D Rahmi, Dini Relsas Yogica, Relsas Renidawati Hutapea Restia Nika Ria Kasmeri RINA M.Si WIDIANA S.Si Rini Silvina Roza, Rizka Khairia Rusdinal, Rusdinal Santosa, Tomi Apra Sarah Sarah, Sarah Selaras, Ganda Hijrah Sherly Serfitria Silvi Susanti Silvina Arifa Siska Adelya Ramadhani Susanti, Silvi Susanti, Wahyu Indah Syari, Elsy Melia Tomi Apra Santosa Tutty Laswardi Yusuf Tutty Laswardi Yusuf Ulfa Syukur Winda A. Fietri Winda Ayu Fietri Wismi Lenggo Geni Witma Novita Atnur Yulia Ratna Sari Yuni Ahda Zikra Zikra Zulyusri Zulyusri