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AKTIVITAS REACTIVE OXYGEN SPECIES MAKROFAG AKIBAT STIMULASI GEL LIDAH BUAYA PADA INFEKSI Salmonella typhimurium Susanti, R.; Yuniastuti, A; Iswari, RS
Indonesian Journal of Mathematics and Natural Sciences Vol 35, No 1 (2012): April 2012
Publisher : Universitas Negeri Semarang

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Reactive Oxygen Species (ROS) merupakan salah satu lethal chemical yang dapatmembunuh dan mengeliminasi bakteri pada sel fagosit. Lidah Buaya (Aloevera) banyak dipakai sebagai pengobatan tradisional, tetapi belum ada buktiilmiah sampai tingkat seluler apalagi subseluler dalam hal efek imunostimulanpada penyakit infeksi. Tujuan penelitian ini adalah untuk mengetahui aktivitasimunostimulan dari gel lidah buaya yang ditunjukkan oleh aktivitas ROS makrofagsecara in vivo terhadap infeksi bakteri patogen Salmonella typhimurium. Sebanyak24 ekor mencit BABL/c betina umur 8-10 minggu berat 20-30 gram dikelompokkansecara acak menjadi empat kelompok, masing-masing kelompok enam ekor.Kelompok kontrol tidak diberi gel Aloe vera, sementara kelompok P1, P2, dan P3berturut-turut diberi gel Aloe vera 0,5 ml/ekor/hari; 1,0 ml/ekor/hari, dan 1,5ml/ekor/hari. Pemberian gel Aloe vera dilakukan selama sembilan hari. Pada harike-6, mencit diinfeksi bakteri patogen Salmonella typhimurium intraperitoneal105 CFU. Selanjutnya pada hari ke-10 mencit didislokasi dan dibedah, diambilmakrofag dari peritoneum untuk dianalisis produksi ROS-nya. Hasil penelitianmenunjukkan bahwa pemberian gel Aloe vera berpengaruh signi..ikan terhadappeningkatan produksi ROS makrofag mencit BALB/c yang diinfeksi Salmonellatyphimurium. Terdapat perbedaan secara signi..ikan antara kelompok kontroldengan kelompok P1, P2, dan P3, tetapi tidak terdapat perbedaan signi..ikan antarkelompok P1, P2, dan P3. Pemberian gel Aloe vera dosis 0,5 ml/ekor/hari sudahmampu meningkatkan produksi ROS makrofag. Reactive Oxygen Species (ROS) is one of lethal chemicals that can kill and eliminatebacteria in phagocytic cells. Aloe vera is widely used as traditional medicine, but thereis no scienti..ic evidence to prove the effect of immunostimulatory of the Aloe vera gel oninfectious disease in the cellular or subcellular level. This research aims to determinethe immunostimulatory activity of Aloe vera gel showed by ROS macrophage activityin vivo on the infection of bacterial pathogen Salmonella typhimurium. This researchused 24 female mice BABL/c age 8-10 weeks weight 20-30 Gram. They were groupedrandomly in four groups consisting of six mice each. The mice in the control groupwere not given the Aloe vera gel, while group P1 was given the gel of 0,5ml/mice/day,group P2 was 1,0ml/mice/day, and group P3 was 1,5ml/mice/day. The gel was givenin nine days, and in 6th day the mice were infected by bacterial pathogen Salmonellatyphimurium then in 10th day the mice were dissected to take their macrophage fromperitoneum for being analyzed its ROS production. The result showed that there wasa signi..icant difference between control group and P1, P2, and P3 group, but there isno signi..icant difference between P1, P2, and P3 group. In conclusion, the dose of 0,5ml/mice/day was able to increase ROS macrophage production.
PROFIL PROTEIN SUSU DAN PRODUK OLAHANNYA Susanti, R.; Hidayat, E
Indonesian Journal of Mathematics and Natural Sciences Vol 39, No 2 (2016): October 2016
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan untuk menganalisis kadar protein dan profil protein pada beberapa susu (susu kedelai, susu kambing) dan olahannya (yogurt, tofu). Kadar protein diukur dengan metode Lowry, sedangkan profil protein dianalisis menggunakan SDS PAGE. Data yang diperoleh dianalisis secara deskriptif. Kadar protein tertinggi pada sampel yang dianalisis terdapat pada produk yogurt A (579,5 mg/ml), disusul susu kedelai (289,99 mg/ml) dan susu kambing (133,1 mg/ml). Analisis profil protein terlihat pita protein dengan mobilitas terendah sampai tertinggi terletak pada berat molekul 14-150 KDa. Pita protein khas yang hanya dimiliki susu kambing adalah pita 150kDa. Sementara pita protein khas yang hanya dimiliki susu kedelai adalah pita 44 kDa dan 55kDa. Pita protein yang khas hanya dimiliki yogurt A (dengan bakteri Lactobacillus bulgaricus dan Streptococcus thermophillus) adalah pita 65Da. Semua jenis susu dan olahannya memiliki pita 70kDa, kecuali susu kedelai. Profil protein susu kedelai dan tofu menunjukkan profil protein yang sangat berbeda, namun keduanya memiliki pita 18kDa.This study aimed to observe protein level and profiles on some milks (soy milk, goat's milk) and dairy (yogurt, tofu) product. Protein content was observed by Lowry method, whereas the protein profiles were analyzed by polyacrylamide gel electrophoresis. Data were analyzed descriptively. The highest protein content of the observed sample was in yogurt A products (579,5 mg/ml), followed by soy milk (289,99 mg/ml) and goat's milk (133,1 mg/ml). Analysis of protein profiles showed protein bands with lowest to highest mobility lies in the molecular weight of 14-150 KDa. Typical protein band of goat's milk was a 150kDa band. While the typical protein bands of soy milk were 44 kDa and 55kDa band. The typical protein band of yogurt A (with Lactobacillus bulgaricus and Streptococcus thermophillus bacterium) was 65Da. All types of milks and dairy had 70kDa band, except for soy milk. Protein profile of soy milk and tofu was very different, but both had 18kDa band.
Download Full Paper Susanti, R.
Indonesian Journal of Mathematics and Natural Sciences Vol 39, No 1 (2016): April 2016
Publisher : Universitas Negeri Semarang

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DAMPAK KORUPSI MELALUI PENDIDIKAN ANTI KORUPSI DALAM MEMBENTUK GENERASI MUDA YANG JUJUR DAN BERINTEGRITAS DI SMA SEMESTA KOTA SEMARANG Handoyo, Eko; Susanti, Martien Herna
Jurnal Abdimas Vol 18, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LP2M), Universitas Negeri Semarang

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Hasil survei lembaga konsultan PERC yang berbasis di Hong Kong menyatakan bahwa Indonesia merupakan negara yang paling korup di antara 12 negara Asia. Belajar dari pengalaman negara lain yang relatif berhasil memberantas korupsi, selain aspek penegakan hukum (law enforcement) yang tidak kalah pentingnya adalah aspek pencegahan dalam bentuk Pendidikan Anti Korupsi (PAK). Tujuan yang hendak dicapai dalam kegiatan ini adalah: 1) Penguatan kesadaran kolektif dampak korupsi melalui pendidikan anti korupsi dalammewujudkan generasi muda yang jujur dan berintegritas di SMA Semesta Kota Semarang, 2) untuk mengetahui hambatan-hambatan yang dihadapi padapenguatan kesadaran kolektif dampak korupsi melalui pendidikan anti korupsi dalam mewujudkan generasi muda yang jujur dan berintegritas di SMA Semesta Kota Semarang. Guna mencapai tujuan tersebut, maka dalam kegiatan ini dilakukan dengan 3 (tiga) kegiatan yaitu: pertama, presentasi arti korupsi, ciriciri korupsi, bentuk-bentuk korupsi, sebab-sebab korupsi dan dampak korupsi. Kedua, penayangan film tentang korupsi. Ketiga, dialog tentang pendidikan anti korupsi dan lembaga-lembaga anti korupsi yang ada di Indonesia.
PENANAMAN NILAI-NILAI KEJUJURAN MELALUI PENDIDIKAN ANTI KORUPSI DI SMA 6 KOTA SEMARANG Handoyo, Eko; Subagyo, Subagyo; Susanti, Martien Herna; Suhardiyanto, Andi
Jurnal Abdimas Vol 14, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LP2M), Universitas Negeri Semarang

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Abstrak. Korupsi di Indonesia bagaikan suatu “penyakit” yang sukar disembuhkan dan merupakan suatu fenomena yang kompleks. Untuk memberantas korupsi di Indonesia tidak cukup hanya dengan melakukan suatu tindakan represif, namun yang lebih mendasar lagi adalah melakukan tindakan preventif atau pencegahan. Salah satu upaya yang dapat dilakukan melalui tindakan preventif ini adalah dengan menumbuhkan kepedulian untuk melawan berbagai tindakan korupsi, dan sekaligus juga mendidik generasi muda dengan menanamkan nilai-nilai etika dan moral yang diperlukan dalam kehidupan bermasyarakat. Banyak hal yang dapat dilakukan, misalnya melalui kampanye publik, maupun melalui penanaman nilai-nilai moral dan etika yang dapat dimasukkan dalam kurikulum pada berbagai level terutama pada level pendidikan awal seperti SD, SMP dan SMA. Dengan upaya ini diharapkan mereka dapat tumbuh menjadi generasi yang “bersih” dan “anti korupsi” sekaligus menjadi contoh bagi generasi sesudahnya dan sebelumnya. Kesadaran dan kepedulian masyarakat perlu ditumbuhkan melalui berbagai cara, antara lain dengan mencanangkan ”Gerakan Anti Korupsi”, yang menandai komitmen berbagai elemen masyarakat dalam memberantas korupsi. Kata kunci: korupsi, penanaman nilai, pendidikan anti korupsi
ANALISIS SEKUEN GEN GLUTATION PEROKSIDASE (GPX1) SEBAGAI DETEKSI STRES OKSIDATIF AKIBAT INFEKSI MYCOBACTERIUM TUBERCULOSIS Yuniastuti, Ari; Susanti, R.
Sainteknol : Jurnal Sains dan Teknologi Vol 11, No 2 (2013): December 2013
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v11i2.5569

Abstract

Glutation merupakan antioksidan yang berperan dalam fungsi imun, dan diekspresikan secara genetik oleh urutan gen yang membentuk protein enzim Glutation Peroxidase (GPx1). Bila ekspresi gen berubah maka terjadi perubahan fungsi glutation dan kerentanan terhadap stress oksidatif. Metode yang digunakan adalah Kasus-kontrol. Sampel yang digunakan adalah sampel darah. Kelompok kasus adalah sampel darah pasien tuberkulosis paru sedangkan kelompok kontrol adalah sampel darah orang sehat. Pemeriksaan gen Glutation peroxidase (GPx1) menggunakan metode Polymerase Chain Reaction (PCR) untuk melihat pita DNA pada pasien tuberkulosis par serta elektroforesis produk PCR-RFLP gen GPx1 kelompok sampel tuberkulosis. Hasil penelitian menunjukkan bahwa tidak terdapat hubungan yang bermakna antara polimorfisme gen GPx1 (p=0,365) pasein tuberkulois dengan individu sehat, sehingga tidak dapat digunakan sebagai alat deteksi kerentanan terhadap stress oksidatif pada pasien tuberkulosis. Perlu penelitian lanjutan yang menggunakan sampel lebih besar dan populasi etnik yang berbeda.
The Effect of Aloe Vera Peel Extract on Histopathology of Rat Pancreas Induced by Alloxan Susanti, R; Setiadi, Eka; Peniati, Endah
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20896

Abstract

The Extract of Aloe Vera Peel (EAVP) contains bioactive compounds (flavonoids, alkaloids, tannins, saponins, and phenolics) that it thought to improve pancreas histopatology on rat with diabetes mellitus, therefore it has potential for diabetes mellitus treatment. This research aimed to figure out the effect of EAVP on improving the histopathology of rat pancreas induced by alloxan. This research was an experimental study using a completely randomized design with a randomized post-test design. A total of 25 rats were divided into 5 groups: C(-) was a normal group, fed and drinking standard; C(+) was positive control group, induced by alloxan 120 mg/kgBW; PI, PII and PIII were groups that were induced by alloxan 120 mg/kgBW and were given a full-dose of EAVP of 87.5, 175 and 350 mg/kgBW respectively. The data was assessed using the Kruskal-Wallis and Mann-Whitney statistical analysis. The results of the statistical test showed that the histopathology of rat pancreas of the C(+) group were significantly different compared with the treatment group. Meanwhile, the representation of histopathology of pancreas between PIII and C(-) were not significantly different. It can be concluded that treating hyperglycemia rats with the EAVP for 28 days can improve the representation of histopathology of rat pancreas. At the laboratory level, EAVP has been shown to repair rat pancreatic damage. With this result, Aloe vera has the potential to be developed as a phytopharmaca for the prevention or treatment of diabetes mellitus.
Upaya Badan Pemberdayaan Perempuan dan Keluarga Berencana (Bppkb) dalam Penanggulangan Kasus Kekerasan Anak Dan Perempuan Di Kabupaten Kendal Firdausi, Aulya Della; Susanti, Martien Herna; Sunarto, Sunarto
Unnes Political Science Journal Vol 2 No 2 (2018): July
Publisher : Program Studi Ilmu Politik Fakultas Ilmu Sosial Universitas Negeri Semarang

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The case of child and woman violence in Kendal District has increased for three consecutive years since 2013. One of the agencies responsible for the prevention of child and female violence cases in Kendal District is the Women's Empowerment and Family Planning Agency (BPPKB). So the the problem in this research are (1) How is the effort of BPPKB in handling child and woman violence case in Kendal District?, (2) What is the cooperation system of BPPKB with other institutions?, and (3) What kind of problems did BPPKB get in handling cases of child and woman violence in Kendal District?. The results of the research show (1) the effort that has been done by BPPKB in handling cases of child and woman violence can be divided into two, prevention and handling after the happening of the case. As a prevention, BPPKB has conducted socialization activities with the material of Law Number 23 Year 2002 regarding Child Protection and Law Number 23 Year 2004 on the Elimination of Domestic Violence. Then for the post-case handling, the victims will be given five services in the form of Reporting Service, Health Service, Advocacy Service, Rehabilitation Service by Shelter and lastly Service of Return and Social Reintegration. (2) Cooperation conducted by BPPKB with other institutions contained in the Decree of Regent of Kendal Number: 463/248/2012 then formed an Integrated Service Team on Handling of Violence Against Women Based on Gender and Child which then formed an organization of Center for Integrated Service of Handling of Violence of Women and Larasati Children (PPT - PKPA Larasati). (3) The problem faced by BPPKB is the lack of human resources and infrastructure facilities. The suggestions that can be given is the addition of the number of human resources officers in both the reporting service and medical services, increasing the intensity of socialization in various layers of society and maintaining cooperation relationship between BPPKB and various related agencies that work together.
The Application of Guided Inquiry using LKPD on Animal Tissue Topic and its Impact to Science Process Skills and Students' Outcome Learning Putri, Amalia Nurina; Susanti, R.
Journal of Biology Education Vol 9 No 1 (2020): April 2020
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v9i1.37869

Abstract

According to interview with biology teacher of 11th grade of SMA Negeri 9 Semarang, known that teaching-learning process on animal tissue topic implemented teacher-centered learning, so that low students’ participation in the classroom happened and also there is no laboratory activity which resulted in the low ability of science process. One way to solve that problem is to implement learning model that carry out scientific methods and student-centered learning called guided inquiry. This study aims to analyze the effectiveness of implementation of guided inquiry model using LKPD on students’ science process skills as well as the learning outcomes. The participants are 72 students of 11th grade in science class 5 and 6 of SMA Negeri 9 Semarang in the first semester 2019/2020 academic year. This study used pre-experimental design with One Shoot Case design. Implementation of guided inquiry model using LKPD is effective if ≥ 90% score of science process skills reach a minimum of good criteria and excellent, ≥ 75% of students are able to reach the minimum criteria for completeness of studying (KKM ≥ 70) and enhancement pretest-posttest (N-gain) score are in the medium-high category of ≥ 90% students. The results show science process skills in 97,15% of students reach good and excellent criteria, completeness of studying score in 93,06% of students reach the minimum criteria for completeness of studying (KKM ≥ 70) as well as the enhancement pretest-posttest (N-gain) score in 100% of students are in the middle to high category. This study has identified guided inquiry model using LKPD of animal tissue topic as effective implementation strategy for developing science process skills of students as well as boosting the learning outcomes of students in SMA Negeri 9 Semarang.
MODEL PENDIDIKAN KEWIRAUSAHAAN DI PERGURUAN TINGGI DALAM MENUMBUHKAN ENTREPRENEUR MUDA KREATIF DAN INOVATIF DI KOTA SEMARANG Susanti, Martien Herna
Forum Ilmu Sosial Vol 41, No 1 (2014): June 2014
Publisher : Faculty of Social Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/fis.v41i1.5377

Abstract

Thought tobring entrepreneurship education in colleges, both of which are integrated in the curriculum and student activities were developed by several universities and is targeted by 2014as many as20 percent of college graduates managed to become a businessman (Institutions of Higher Education,December 22, 2009). The creation of community among faculty and entrepreneurs from college graduatesis intended to accelerate the addition of Indonesian business man who needed to drive economic growth in the nation. Associated with improving the quality and relevance of education which basically includes the development of the moral aspects, morals, mind, character, knowledge, skills, health, art and culture, the development of these aspects, leads to improvement and development of life skills, which is realized through the achievement of basic competencies to survive, and able to adapt them selves to succeed in life. But in fact the institution has not bee nable to resolve the problem of unemployment and bridging the world of education and business world.
Co-Authors - Suprapti, - A Yuniastuti A.A. Ketut Agung Cahyawan W Adhi, Nino Aditya Marianti Aji Purwinarko, Aji Ali Rizqi, Nur Kholish Ali Rizqi, Nur Kholish Ana Fitria Ananda, Ananda Anandha Anandha, Anandha Andi Suhardiyanto Andreas Priyono Budi Prasetyo Angga Ari Wibowo Ani Rusilowati Ari Yuniastuti Ariesta, Putri Ariesta, Putri ARIF INDIARTO, ARIF Arlinda, Dyken Dwi Asri, Widi Aulia, Innarotul Aulia, Maliqa Azkiyah, Nimas Catur BAHRAIN DWI MASITHO, BAHRAIN DWI Bahtiar, Denny Herbianto Cahya Buana Cahyati, Widya Hari Cahyo, Reza Dwi Cahyo, Reza Dwi Chasnah Chasnah, Chasnah Chatarina Umbul Wahyuni Christijanti, W. Dafip, M. Dewi Mustikaningtyas Dewi, Nuriana Rahmani DIAN LESTARI Dimarti, Safira Chaerani Dimarti, Safira Chairani Dita Selviana, Dita Doni Saputro E Hidayat, E E.S.,, Sri Mulyani Endah Peniati ENDANG TRI MARGAWATI Enni Suwarsi Rahayu F Widhi Mahatmanti Fachry Abda El Rahman Falistina, Amna Farikhul Ulum Felintina Yuniarti, Felintina Fidia Fibriana Fifti Istiklaili, Fifti Firdausi, Aulya Della Fitri Arum Sasi, Fitri Arum Fitriya, Farikhatun Nur Gita Ria Octafia, Gita Ria Hammam, Bagus Hardi Saputro, Iwan Hasani, Azharu Alfi I Gusti Ngurah K Mahardika I Gusti Ngurah Kade Mahardika I Wayan T Wibawan I-W.T Wibawan Ibnul Mubarok Indriawati, Indriawati Irna Kinayungan, Irna Isnaeni, Lili Astuti Isnaeni, Lili Astuti Isti Hidayah Iswari, R S Jaya, Adi Franata Kenarni, Naina Rizki Kristamtini, K Kusuma, Fitriana Dian Kusuma, Putri Patria Larasanti, Firna Larasanti, Firna Lina Herlina Listiaji, Prasetyo M.T Suhartono Maggy T Suhartono Mahardika Prasetya Aji Maman Rachman Margareta Rahayuningsih Nugroho Edi K Moh. Aris Munandar, Moh. Aris Mohamed, Mohd Salim Bin Mukaromah, Riska Laila Muyasaroh, Kun Maisarotun Na’ima, Mirtaati Nahar, Akhmad Lutfi Nahar, Akhmad Lutfi Naim, Fatchun Nezar, Muhammad Rofiq Ning Setiati Nisa, Farah Fitrotun Nissa, Ma’isyatun Nugrahaningsih Nugrahaningsih Nugrahaningsih WH, Nugrahaningsih Nur Halimah Nur Kusuma Dewi Nur Rahayu Utami Oktaviani, Esa Pauline, Maretha Putri Pramesti Dewi Pratiwi, Retno Herningrum Priyantini Widiyaningrum PUJI LESTARI Pukan, Krispinus Putri, Amalia Nurina Putri, Komala Amelia R, Rahmatika Saputri R.D Soejoedono RAHMA FERDIANA, RAHMA Rahmatih, Aisa Nikmah Rahmatih, Aisa Nikmah Rahmawati, Esa Mara Rahmawati, Sinta Kurnia Rakainsa, Senda Kartika Ratih Puspita Dewi, Ratih Puspita Retna Utami Retno D Soejoedono Retno Sri Iswari Rizki Puspita Dewi, Rizki Puspita Rohmah, Amalia Nor Romadhoni, Mar`atush Sholihah RS Iswari S.Pd. M Kes I Ketut Sudiana . Sa’adah, Siti Rofiatus Salfia, Mega Salwa, Khoerina Samsul Nizar Saputri, Aprilia Rizki Saraswati, Ifah Saraswati, Ifah Septiana Anggraini, Septiana Setiadi, Eka Setiajid Setiajid Setiajid Setiajid, Setiajid Shinta Widyastuti, Shinta Sigit Saptono Siti Harnina Bintari Sri Sukaesih Sri Utami Sriyadi Sriyadi, Sriyadi Subagyo Subagyo Sulistyo, Danu Sumarno . Sunarto Sunarto Supriyanto Supriyanto Suryo Pratikwo Suryonegoro, Bayu Murti Sutikno Sutikno Tafrika Donita Talitha Widiatningrum Tijan Tijan, Tijan Tri Budi Kurniawan, Tri Budi Trisna, Mila Tuti Widianti Umi Isnaeni, Umi Utami, Anisa Dyah Utami, Melia Dwi Utomo, Didik Huswo Wahyuni, Desiy Ema Dwi Wardono, Hari Rudijanto Indro Widya Hary Cahyati Widyaningrum, Kartika Wijaya, Ifan Badra Wijayanti , Yuni Wiwi Isnaeni Wulan Christijanti Wulandari K. E, Yenni Tyas Yohanes Nong Bunga Yuliana, Lusia Retno Yuliana, Lusia Retno Yuliantika, Atika Yuni Wijayanti Yuniastuti , Ari Yuniastuti, A. Yustinus Ulung Anggraito Zaenafree, Intan Zakyani, Nova N