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Isolation and Screening Microorganisms from Black Soldier Fly Larvae (Hermetia illucens) that Producing Amylase, Protease and Cellulase Dalia Sukmawati; Rheva Amadea; Putri Novitasari; Yohana Caroline Sihombing; Axel Mareta; Yohannes Eka Cordias Buulolo; Atin Supiyani; Muktiningsih Nurdjayadi; Diat Nurhidayat; Roshanida A. Rahman; Sulistiyani; Raden Haryo Bimo Setiarto
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4516

Abstract

The Black Soldier Fly (BSF) has gained significant attention for its ability to decompose various types of organic waste and produce valuable enzymes related to its digestive system. These enzymes hold immense potential for applications across diverse industrial sectors, with promise in the livestock industry. This study aimed to extract and assess amylase, protease, and cellulase enzymes from microorganisms present in BSF larvae fermentation. The isolation technique employed was the spread plate method, followed by rigorous screening for the presence of these enzymes. The results showed 46 isolates of 26 bacterial isolates and 20 yeast isolates. In the amylase enzyme screening, a substantial 25 bacterial and 19 yeast isolates exhibited positive amylase activity. For cellulase, 20 bacterial and 14 yeast isolates displayed positive results. In the case of protease, 16 bacterial and 12 yeast isolates demonstrated protease enzyme activity. Notably, nine isolates exhibited the remarkable capability to produce multiple enzymes, including eight bacterial and one yeast isolate. These results showcase the rich enzymatic potential of BSF-associated microorganisms, offering exciting prospects for their application in various industrial sectors, especially in enhancing the efficiency and sustainability of livestock production
Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction Muktiningsih Nurjayadi; Gladys Indira Putri; Jefferson Lynford Declan; Ismaya Krisdawati; Dandy Akbar Juliansyah; Maharanianska Azzahra; Irvan Maulana; Irma Ratna Kartika; Fera Kurniadewi; Tiara Fahriza; Adinda Myra Amalia Putri; Ayu Berkahingrum; Atikah Nur Rahmawati; Rosita Gio Anggraeni; Dalia Sukmawati; Sri Rahayu; Vira Saamia; I Made Wiranatha; Bassam Abomoelak; Hesham Ali El-Enshasy
Acta Biochimica Indonesiana Vol. 7 No. 1 (2024): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.159

Abstract

Background: Seafood is highly nutritious but poses health risks when contaminated with pathogenic bacteria like Vibrio alginolyticus, which causes food poisoning and can infect marine animals and humans. Objective: This research aimed to determine the sensitivity and specificity of real-time polymerase chain reaction (rt-PCR) using the flgL primer pair to detect V. alginolyticus bacteria in seafood. Methods: The rt-PCR method was used to detect V. alginolyticus quickly, specifically, and sensitively. The flgL primer pair was evaluated for amplicon length, Ct value, Tm value, and its ability to differentiate between target and non-target bacteria. In this research, the samples tested were red snapper and blood clams. Results: The flgL primer produced an amplicon length of 224 bp. At 50 ng concentration, it yielded a Ct value of approximately 11.00 and a Tm of approximately 83°C. The flgL primer successfully differentiated between target and non-target bacteria. In sensitivity tests, it detected V. alginolyticus at concentrations as low as 1.86 x 10-3 ng/µL. Detection in seafood samples was also successful. Conclusion: The rt-PCR assay using the flgL primer pair effectively detects Vibrio alginolyticus in seafood with high specificity, sensitivity, and rapidity. These findings support its use for rapid and accurate detection of pathogenic bacteria in seafood.
Desiminasi Pengembangan Media Pembelajaran Modul Elektronik Kimia Generasi ke-4 pada Guru Kimia di Wilayah Jakarta Timur 2 Nurjayadi, Muktiningsih; Irma Ratna Kartika; Irwan Saputra; Siti Fatimah; Ririn Gustini; Uswatul Nisa; Rizkahana Syehfia; Nida Nur Afifah; Jefferson Lynford Declan; Pardiana; Sintia Mardita
Bahasa Indonesia Vol 21 No 01 (2024): Sarwahita : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/sarwahita.211.5

Abstract

generation electronic module is one of the chemistry learning media that can facilitate a three-way interaction between students, teachers and learning resources in 5.0 society era. Various chemical materials can be presented in the form of electronic modules equipped with applications according to the characteristics of the material. However, these developments are often not followed by the development of teacher competence in the field. So that there is a gap between scientific developments in Higher Education and conditions in the field. In this community service program, dissemination of the development of the 4th generation chemistry e-module has been carried out for MGMP-2 (Musyawarah Guru Mata Pelajaran) teachers in the East Jakarta Region through the dissemination of research results conducted in the Chemistry Education Program of FMIPA UNJ, that carried out face to face, starting with the delivery of material, Workshop, interactive discussion and coaching online blended learning. During the process of delivering the material, the participants enthusiastically followed it step by step. At the end of the session an evaluation questionnaire was given, the input and suggestions obtained were very positive. So, it is hoped that this activity can continue to be carried out and be useful in disseminating science and technology, increasing teacher competence and adding development information in tertiary institutions according to field needs. Abstrak Perkembangan teknologi sangat mempengaruhi perkembangan media pembelajaran kimia saat ini. Elektronik modul generasi ke-4 merupakan salah satu media pembelajaran kimia yang dapat memfasilitasi interaksi tiga arah antara siswa, guru dan sumber belajar di era society 5.0. Berbagai materi kimia dapat disajikan dalam bentuk modul elektronik yang dilengkapi dengan aplikasi sesuai karakteristik materinya. Namun perkembangan media pembelajaran tersebut sering tidak diikuti dengan perkembangan kompetensi guru di lapangan. Sehingga terjadi kesenjangan antara perkembangan keilmuan di Perguruan Tinggi dengan kondisi dilapangan. Pada program pengabdian pada masyakarat ini, telah dilakukan desiminasi pengembangan e-modul kimia generasi ke-4 bagi guru MGMP-2 (Musyawarah Guru Mata Pelajaran) Wilayah Jakarta Timur melalui desiminiasi hasil penelitian yang dilakukan di rumpun Kimia FMIPA UNJ yang dilakukan seacara tatap muka, dimulai dengan penyampaian materi, Workshop, diskusi interaktif dan pembinaan secara blanded learning. Selama proses penyampaian materi, peserta antusias mengikuti tahap demi tahap. Pada akhir sesi diberikan angket evaluasi, masukan dan saran yang didapat sangat positif. Sehingga diharapkan kegiatan ini dapat terus dilakuakan dan bermanfaat dalam menyebarluaskan IPTEK, meningkatkan kompetensi guru serta menambah informasi pengembangan di Perguruan Tinggi sesuai dengan kebutuhan dilapangan.
Development of Bacillus subtilis Detection Method Targeting Genes codY, narH, and ureC Using Polymerase Chain Reaction Nurjayadi, Muktiningsih; Berkahingrum, Ayu; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Anggraeni, Rosita Gio; Fahriza, Tiara; Declan, Jefferson Lynford; Putri, Gladys Indira; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; El Enshasy, Hesham
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.26

Abstract

Bacillus subtilis, a causative agent of foodborne illness in bread, cakes, cereals, and cheese leading to symptoms like diarrhea and nausea, was investigated for its role in nosocomial infections, including endocarditis, bacteremia, septicemia, and meningitis. The research focused on three genes: codY, narH, and ureC, which respectively contribute to bacterial survival through biofilm and spore formation, acid resistance, and adaptation to anaerobic conditions. The objective of this study was to evaluate the codY, narH, and ureC genes using the Gradient PCR method for Bacillus subtilis detection in bread and cheese through Real-Time PCR. During the research, bacterial growth was observed with an OD600 of 1.438 in Tryptic Soy Broth (TSB), and colonies of medium size, smooth, cream-coloured, and round shape were successfully isolated on Tryptic Soy Agar (TSA). The DNA template used had a 71.5 ng/µL concentration with an A260/280 ratio of approximately 1.830. The annealing temperature for Gradient PCR used was 53-62°C. The primers successfully amplified codY (175 bp), narH (222 bp), and ureC (153 bp) gene amplicons. The optimal annealing temperature for the primers used was 60°C, as indicated by the presence of a single bright band in electrophoresis. Using these three different genes, testing can also be conducted with the Multiplex PCR method. The next step involves developing a detection kit using optimized primers and annealing temperatures for the identification of Bacillus subtilis in bread and cheese samples using Real-Time PCR.
Garciniaxanthone E and 12b-Hydroxy-des-D-garcigerrin A from The Tree Bark Garcinia dulcis and their Inhibitory Properties against Receptor Tyrosine Kinases Kurniadewi, Fera; Aqilah, Amadita Shafa; Kartika, Irma Ratna; Nurjayadi, Muktiningsih; Hermawati, Elvira; Danova, Ade
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 1, May 2024
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i1.38159

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Two xanthone derivatives, garciniaxanthone E (1) and 12b-Hydroxy-des-D-garcigerrin A (2) have been isolated from ethyl acetate extract of the tree bark of Garcinia dulcis. The Ultraviolet (UV), Infrared (IR), Nuclear Magnetic Resonance (NMR), and Mass Spectrometry (MS) data analysis elucidated the structure of the isolated compounds. This study represents the first evaluation of compounds 1 and 2 in terms of their efficacy against receptor tyrosine kinases. The results showed that compound 1 exhibited weak activity with 12% inhibition against Insulin Receptor (InsR), while compound 2 showed moderate activity with 29% inhibition against epidermal growth factor receptor (EGFR). A molecular docking study targeting EGFR-TK suggests that the hydroxyl group at C-4 on compound 2 can be demolished to raise the inhibitory activity in future research.
Limit Detection of Short Tandem Repeats (STR) Analysis on Touch DNA Samples Saamia, Vira; Yudianto, Ahmad; Nurjayadi, Muktiningsih; Novitasari, Novitasari; Furqoni, Abdul Hadi
Indonesian Journal of Chemistry Vol 24, No 5 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.94081

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Forensic short tandem repeats (STR) profiling on touch DNA samples has emerged as a primary method for human identification. The stability and uniqueness of STR combination from the targeted locus in each individual make it a precision marker for human identification. Touch DNA samples can be found in traces of biological material shed from a person. This work aimed to identify the lowest concentration limit required for generating an interpretable DNA profile and the sensitivity of the STR loci applied. Touch DNA samples were collected from donors who were asked to hold a rope for 5 min. A double swab technique was used to lift the touch samples from the rope. These samples are subjected to DNA extraction and quantification. Two STR amplification cycles, 29 and 34 cycles, were used. DNA concentration greatly influences the success of amplifying the target allele at each STR locus to be interpreted into a complete DNA profile, shown by its allele peak. Touch DNA concentration >0.25 ng can produce a complete DNA profile. LCN method successfully amplified touch DNA with a concentration 0.0625–0.25 ng/µL. Limit detection of touch DNA analysis is 0.25 ng/µL. Low-copy DNA can still be analyzed within 0.0625–0.25 ng/µL.
Pengembangan Kit Small-Scale dan Flipbook Petunjuk Praktikum Asam Basa Ely Puspita Sari; Fera Kurniadewi; Muktiningsih Nurjayadi
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.7586

Abstract

Practicum is essential to learning because it provides practical learning opportunities in building students' knowledge. Learning using the practical method can explain abstract theories into concrete ones and can be analyzed in more detail so that this method can improve cognitive, psychomotor, and affective skills. Practical activities in schools currently still use large amounts of chemicals. The resulting waste is only thrown in the trash or the drain. Disposal of large amounts of chemical waste will affect the surrounding environment. Based on this background, the researcher aims to develop a small-scale practical kit and flipbook for instructions on practical acid and base solutions. This type of research is development research (R&D) and uses the ADDIE development model (Analysis, Design, Development, Implementation, and Evaluation). Material and language experts and media experts validate products. Product trials were carried out at SMAN 15 Tangerang Regency. The results of the validation of the small-scale practicum kit and flipbook practicum instructions were material 87% (very feasible), language 80% (feasible), and media 84% (very feasible). The product trial results stated that the small-scale practical kit and practical flipbook instructions were suitable for use in the learning process
Touch DNA viability on various substrates from different shedder levels Saamia, Vira; Yudianto, Ahmad; Nurjayadi, Muktiningsih; Novitasari
Medical Journal of Indonesia Vol. 33 No. 3 (2024): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247398

Abstract

BACKGROUND Touch DNA samples are frequently discovered at crime scenes, including those found at the scene, on the victim, with the suspect, or on objects related to the incident. This study aimed to investigate 3 key factors affecting touch DNA samples: the characteristics person that shed the DNA, surfaces variants where the DNA was deposited, and different sampling methods effectiveness that influence DNA quantity, quality, and detection. METHODS 9 participants grouped into high, intermediate, and low shedder levels simultaneously tied 2 types of ropes, non-porous and porous. The first person will hold a rope for 5 min then pass it to the second person to hold on the same spot for another 5 min. DNA was collected from each rope using the double swab and tape-lift method, extracted, and quantified using real-time polymerase chain reaction (PCR). Touch DNA profile at 20 short tandem repeat loci was amplified in PCR system and detected on capillary electrophoresis. RESULTS Type of substrate (p = 0.97) or sampling method (p = 0.053) used for touch DNA collection did not significantly impact the DNA yield or profiling outcomes. A notable difference (p<0.001) was found in DNA quantity between high, intermediate, and low shedders, regardless of the substrate or method used. CONCLUSIONS Individual shedder level has a greater influence on the results of touch DNA analysis regarding the DNA quantity and profiling quality than substrate type and sample procedure.
PELATIHAN PEMBUATAN YOGHURT UNTUK GURU-GURU SMA KIMIA DI MGMP JAKARTA TIMUR 2 Irma Ratna Kartika; Fera Kurniadewi; Muktiningsih Muktiningsih; Fathya Putri Fajriani; Kinanti Istantia Chantika; Elsa Septiani; Isfasona Bunga Falana; Sheila Nurul Fadillah Octaviany
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 7, No 4 (2023): December
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v7i4.19645

Abstract

ABSTRAKKementerian Pendidikan dan Kebudayaan Republik Indonesia sejak Februari 2022 telah menerbitkan Kurikulum Merdeka Belajar yang bertujuan untuk mengatasi ketertinggalan dan kesenjangan pembelajaran karena pandemi COVID-19. Penerapan Kurikulum Merdeka Belajar pada pembelajaran Sains memberi kesempatan para peserta didik untuk lebih melibatkan panca indra dan belajar lebih dekat dengan kesehariannya (muatan lokal) melalui pembelajaran lintas disiplin ilmu, yang tidak terakomodasi oleh mata pelajaran nasional. Oleh karena itu, perlu adanya kegiatan untuk meningkatkan keterampilan dan kreativitas guru tentang praktikum pembelajaran sains di sekolah dan kaitannya dengan pembelajaran kontekstual, yaitu pembuatan yoghurt. Yoghurt diyakini dapat meningkatkan imunitas tubuh saat dan setelah pandemik COVID-19 supaya sistem pencernaan menjadi sehat karena meningkatnya kekebalan tubuh. Pelatihan pembuatan yoghurt telah dilakukan oleh tim PkM KKN UNJ terhadap 35 guru kimia dari MGMP Kimia Jakarta Timur 2 di SMAN 39 Jakarta dan SMAN 81 Jakarta. Peserta kegiatan sangat bersemangat dan memiliki antusiasme tinggi selama kegiatan berlangsung. Kegiatan dapat meningkatkan keterampilan dan kesadaran guru-guru terhadap pentingnya pembuatan dan manfaat yoghurt untuk meningkatkan imunitas tubuh saat dan pasca pandemik COVID-19. Guru-guru SMA termotivasi untuk sharing dan menyampaikan informasi dan pengetahuan tentang yoghurt kepada masyarakat sekitar lingkungan dan penerapan Kurikulum Merdeka Belajar pada pembelajaran sains kontekstual yang digabung dengan pendekatan disiplin ilmu dan pendekatan pembelajaran berbasis eksperimen. Kata kunci: kurikulum merdeka belajar; covid-19; MGMP kimia; yoghurt. ABSTRACTSince February 2022, the Ministry of Education and Culture of the Republic of Indonesia has published the Independent Learning Curriculum which aims to overcome learning loss and learning gap due to the COVID-19 pandemic. Application of its Curriculum into Science learning provides opportunities for students to involve five senses more and learn closer to their daily lives (local content) through cross-disciplinary learning, which is not accommodated by national subjects. The application of the Curriculum to learning Science provides opportunities for students to involved the five senses more and learned closer to their daily lives (local content) through cross-disciplinary learning, which is not accommodated by national subjects. Therefore, it is necessary to perform activities to improve the skills and creativity of teachers regarding science learning practicum in schools and its relation to contextual learning, namely yogurt production. Yogurt is believed to be able to increase the body's immune system during and after the COVID-19 pandemic into a healthy digestive system. Yogurt-production training was conducted for 35 chemistry teachers of East Jakarta 2 Chemistry MGMP members at SMAN 39 Jakarta and SMAN 81 Jakarta. The activity participants were very excited and enthusiastic during the activity. The current activity can enhance the teacher’s competence and awareness of yogurt benefits to increase body immunity during and after the COVID-19 pandemic. The teachers are motivated to share yogurt information and knowledge with the local community. They also intend to apply the combination of an Independent Learning Curriculum to contextual science learning, a scientific discipline approach, and an experiment-based learning approach. Keywords: independent learning curriculum; covid-19; chemistry MGMP; yogurt.
Pengaruh Model Pembelajaran Guided Discovery Learning Terhadap Literasi Kimia Peserta Didik Pada Materi Larutan Penyangga Darwis, Darsef; Permatasari, Nur Azmi; Nurjayadi, Muktiningsih
Jurnal Riset Pendidikan Kimia (JRPK) Vol. 9 No. 2 (2019): Jurnal Riset Pendidikan Kimia (JRPK), Volume 9 Nomor 2 (2019)
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.094 KB) | DOI: 10.21009/JRPK.092.02

Abstract

The purpose of this research was to know the effect of guided discovery learning models on students' chemical literacy in the buffer solution material. This research was conducted at SMAN 53 Jakarta in the even semester of the 2018/2019 academic year. The research method was quasi experiment. The sample in this study was selected through purposive random sampling. The study sample consist of two classes, XI MIPA 4 as the control class and Class XI MIPA 3 as the experimental class. The control class gets learning with discussion methods and the experimental class gets learning with guided discovery learning models. Based on the results of data analysis, the value of tcount is 5.568 and the value of ttable is 2.001 with df = 59 and the significance level is α = 0.05. The results of this study indicate that the tcount is greater than ttable (5.568> 2.001), it can be concluded that there is a positive influence on the application of guided discovery learning learning models to students' chemical literacy in the buffer solution material. Keywords Guided Discovery Learning Model, Chemical Literacy, Buffer Solution
Co-Authors Abdul Hakam Barok Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Achmad Ridwan, Achmad Ade Danova Adinda Myra Amalia Putri Adinda Myra Amalia Putri Agung Purwanto AGUS SETIAWAN Agus Setiawan Ahmad Yudianto Aina Q Marof Akbar, Dandy Alin Mardiah Aliyah, Elvira Zulfah Ananda Indah Putri Sihombing Angelina, Helzi Anggraeni, Rosita Gio Anisa Fitriyanti Annisa Auliya Annisa Maharani Annisa, Cindy Ermintia Aqilah, Amadita Shafa Arief Rahmad Maulana Akbar Arieztania Rahmadhani Arif Rahman Asri Sulfianti Athiyah Layla Atikah Nur Rahmawati Atin Supiyani Aulia Siti Pathoni Aulia Widowati Axel Mareta Ayu Berkahingrum Azidi Irwan Azizah, Puan Aqila Azzahra, Maharanianska Bassam Abomoelak Bassam Abomoelak Berkahingrum, Ayu Cahyani, Septia Dwi Chen, Shyi-Tien Dahlia Dahlia Dalia Sukmawati Dandy Akbar Juliansyah Dandy Akbar Juliansyah Dandy Akbar Juliansyah Daniel Joe Dailin Darwis, Darsef Desi Lestari Dessy Natalia Devi Aliefiyardi Dian Larasati Diana Vivanti Diat Nurhidayat Dudi Hardianto Dwi Ana Oktaviani El Enshasy, Hesham El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Elsa Septiani Ely Puspita Sari Enshasy, Hesham Ali El Erdawati Ezzaty NM Razif Fahriza, Tiara Farid Agouillal Fathya Putri Fajriani Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurniadewi Fera Kurniadewi Fera Kurniadewi Fernita Puspasari Fitri W Fitriyanti, Anisa Furqoni, Abdul Hadi Geta Putri Mentari Giri Alviyanto Gladys Indira Putri Gladys Indira Putri Gladys Indira Putri Grace Grace, Grace Gusti Angieta Putri Hairishah, Nazrisya Helzi Angelina Hermawati, Elvira Hesham A. El Enshasy Hesham Ali Al-Enshashy Hesham Ali El Enshasy Hesham Ali El-Enshasy I Made Wiranatha I Made Wiranatha I Nyoman Ruja IMAM SANTOSO Imanuelle Orchidea R P Ira Nur Amalia Irma Ratna Kartika Irvan Maulana Irvan Maulana Irwan Saputra Isfasona Bunga Falana Ismaya Krisdawati Ismaya Krisdawati Ismaya Krisdawati Izzatul Ilma Chairinnisa Jefferson Lynford Declan Johanes Erwin Tantaruna Juliansyah, Dandy Akbar Kartika, Irma Ratna Kinanti Istantia Chantika Krisdawati, Ismaya Kurnia Agustini Kurnia Agustini Kurniadewi, Fera Lanasari, Rossy Lilis Septyarini Lim J. R Listya Ayu Saraswati Maharani Azka Azzahra Maharanianska Azzahra Maria Paristiowati Masturdin Masturdin Maulana, Irvan Mellyna Fitriani Musie, Royna Rahma Nabilla Anisa Putrie Nasywa Fhelia Salta Nida Nur Afifah Nita Aresanti Novi Permanasari Novi Wulandari Novianti Mantasha NOVITASARI Novitasari Novitasari Oni Soesanto Soesanto Pardiana Permatasari, Nur Azmi Puan Aqila Azizah Putri Novitasari Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Raden Haryo Bimo Setiarto Rahma, Wanda Amelia Rahmat Yunus Yunus Rahmawati, Atikah Nur Ramadhan, Fitri Nur Rara Seruni Rara Seruni Retno Dwi Cahayningrum Rheva Amadea Rina Agustina Ririn Gustini Rizkahana Syehfia Rizki Fitria Roshanida A. Rahman Rosi Nurhujaimah Rosita Gio Anggraeni Royna Rahma Musie S, Restu.N S Saamia, Vira Salsabila, Nadia Sara E Gomaa Sarah Adilisa Kartini Sari, Ely Puspita Setia Budi Shangkara, Muhammad Arkent Sheila Nurul Fadillah Octaviany Shyi-Tien Chen Sihombing, Ananda Indah Putri Sindy Kania Sulastri Sintia Mardita Siti Azizah SITI FATIMAH Siti Fatimah Siti Munawaoh Siti Munawaroh Siti Namiran Hadis Sondang N. Sihombing Sri Rahayu SRI RAHAYU Sulistiyani Susilo, Ahmad Dwi Susilo, Tanto Budi Syamsul Bachri Tiara Fahriza Toton Sutono Tri Hastuti Budi Utami Tri Susanti Ucu Cahyana Ulfah Choiriyah Uswatul Nisa Vira Saamia Vira Saamia Vira Saamia Wahyuningtiyas, Kartika Putri Wiranatha, I Made Yohana Caroline Sihombing Yohannes Eka Cordias Buulolo Yuli Rahmawati Zahroni, Siti Zarani M Taher