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Real-time PCR-based Detection of Foodborne Pathogen Cronobacter sakazakii DNA in Infant Formula Milk with Specific Targeting on the hfq Gene Nurjayadi, Muktiningsih; Juliansyah, Dandy Akbar; Declan, Jefferson Lynford; Putri, Gladys Indira; Krisdawati, Ismaya; Rahmawati, Atikah Nur; Azzahra, Maharanianska; Maulana, Irvan; Putri, Gusti Angieta; Kurniadewi, Fera; Kartika, Irma Ratna; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1597-1607

Abstract

Cronobacter sakazakii has been linked to cause meningitis, necrotizing enterocolitis, and sepsis in infants and newborns, with case fatality rates ranging from 40 to 80%. The most common source of infection has been identified as Cronobacter sakazakii-contaminated infant formula. With a relatively specific target hfq gene, this study aims to develop a real-time PCR method to identify Cronobacter sakazakii in infant formula milk. Real-time PCR is used as a detection method because rt- PCR has higher specificity and sensitivity compared to conventional PCR methods. The real-time PCR method also has a higher level of effectiveness and time efficiency compared to conventional PCR. Cronobacter sakazakii ATCC 29544 genomic DNA was isolated and used in a real-time PCR assay. Cronobacter sakazakii DNA was amplified using a primer targeting the hfq gene, yielding a 145 bp amplicon. The results of the real-time PCR test showed that Cronobacter sakazakii DNA with a concentration of 53 ng/µL could be amplified by the primer pairs of hfq gene with Ct values of 11 respectively then had Tm values of 81.7°C±0.5. The specificity test showed that the hfq primer pairs could differentiate between the target and some non-target bacteria. The sensitivity test showed the ability of the primer to detect the smallest concentration of 3.392 pg/µL with a Ct of 26.16. Based on the results obtained, it can be concluded that the hfq primer has the potential to be used as a fast detection method for Cronobacter sakazakii bacteria in infant formula using real-time PCR.
Assessing Methods for Enhanced Recovery of Touch DNA from Fingerprints: A Pilot Study Saamia, Vira; Yudianto, Ahmad; Nurjayadi, Muktiningsih; Novitasari
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.79-86

Abstract

The rapid advancement of science and technology, particularly in forensic science, has significantly enhanced crime investigation methodologies. One such advancement is the utilization of Scientific Crime Investigation methods, specifically the analysis of touch DNA from fingerprints. This research investigates the efficiency of fingerprint powders and swabbing agents in improving the quality and quantity of touch DNA for forensic applications. Touch DNA, derived from cellular materials like sweat and skin cells, presents a valuable source of genetic material for identification purposes. The study involved experimental analyses using Regular Silk Black Fingerprint Powder and Magnetic Dual-Purpose Powder, coupled with non-ionic detergent surfactants as swabbing agents. DNA samples were collected from volunteers with varying DNA shedding levels, processed, and analyzed using quantitative PCR and capillary electrophoresis. Results indicated that fingerprint powders significantly reduce the quantity and quality of recovered DNA due to DNA damage caused by the powders' chemical composition. Conversely, using non-ionic surfactants like Triton™ X-100 in swabbing improved DNA recovery and stability, leading to more complete DNA profiles. This study underscores the importance of optimizing fingerprint powder formulations and DNA sampling techniques to enhance forensic DNA analysis. The findings advocate for the development of less damaging fingerprint powders and improved DNA extraction protocols to preserve the integrity of touch DNA evidence in forensic investigations.
hpmA Gene as a Detection Method of Proteus mirabilis Bacteria using real-time Polymerase Chain Reaction Nurjayadi, Muktiningsih; Putri, Gladys Indira; Fitriyanti, Anisa; Declan, Jefferson Lynford; Novitasari; Abomoelak, Bassam
Jurnal Kesehatan Masyarakat Vol. 21 No. 2 (2025)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v21i2.16214

Abstract

Proteus mirabilis is pathogenic bacteria that can cause gastrointestinal infections, bacteremia, and Urinary Tract Infections (UTI). Therefore, it is necessary to have a fast, sensitive, specific, and accurate detection method to identify Proteus mirabilis. This study aims to determine the confirmation, specificity, and sensitivity test of the hpmA gene primer to detect Proteus mirabilis quick and precise using the real-time Polymerase Chain Reaction method. Gradient Polymerase Chain Reaction results showed the hpmA primer has an amplicons length of 195 bp and the optimum annealing temperature at 60°C. The primer pair produced Ct value of 10.40±0.18 and showed one peak in the melting curve with Tm value of 81.84°C±0.02 by real-time PCR. Furthermore, the hpmA primer was also able to distinguish target and non-target bacteria based on the difference in Ct and Tm value formed. Based on these results, the concentration of bacterial DNA that can be detected by primers reached 3.2 pg/μL, equivalent to the concentration of target bacteria that can be detected by primers is 10.24×102 CFU. In the next step, hmpA primer will be developed to detect Proteus mirabilis in artificial contaminated samples using real-time PCR.
Efficacy Test of Prototype Kit for Detection Bacillus cereus and Listeria monocytogenes in Processed Meat using Real-time PCR Method Nurjayadi, Muktiningsih; Fahriza, Tiara; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Berkahingrum, Ayu; Anggraeni, Rosita Gio; Putri, Gladys Indira; Declan, Jefferson Lynford; Akbar, Dandy; Maulana, Irvan; Azzahra, Maharanianska; Shangkara, Muhammad Arkent; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abumoelak, Bassam; El Enshasy, Hesham
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.44280

Abstract

According to the World Health Organization (WHO), harmful agents such as Bacillus cereus and Listeria monocytogenes are responsible for 600 million cases of disease and 420,000 deaths annually. This research aims to test the effectiveness of the real-time PCR method for developing a prototype kit to detect pathogenic bacteria in processed meat. As a comparison, the and conventional PCR methods were used to obtain the accuracy, specificity, sensitivity, and effectiveness of the real-time PCR method. All the samples were cultured in solid media agar, performed amplification using specific primers cyt-K 2 and hly using PCR and real-time PCR. Meatballs, nuggets, and sausages, five samples each, were found to be positive positively contaminated with all targeted bacteria. However, they did not provide specific results using solid media culture and the PCR method. In addition, the real-time PCR method using prototype kit formulas accomplished that all contaminated samples had a Ct value smaller than the negative control, NTC (No Template Control), and had a similar melting curve to the positive control. This establishes that the real-time PCR method clarifies that all samples were contaminated with target bacteria. A formula was developed in the prototype kit with real-time PCR methods that can be used and applied on a commercial scale efficiently and precisely.
Development of Interactive E-Modules Based on POGIL (Process Oriented Guided Inquiry Learning) on Stoichiometry Material in Senior High School Zahroni, Siti; Erdawati; Nurjayadi, Muktiningsih
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.8244

Abstract

This research aims to produce a POGIL-based interactive chemistry e-module for chemistry learning in chapter stoichiometry or chemical calculation. This type of research is Research and Development (RnD) and the Borg and Gall development model. The subjects of this research were XI grade high school students. Product validation was carried out by material and language and media experts. The application used is Flip PDF Professional. The product trial was conducted at SMAN 71 Jakarta. The results showed that: (1) Expert assessment related to material aspects amounted to 85% with good interpretation, language aspects 85.83% good interpretation, and media aspects 82.21% good interpretation. (2) Assessment by teachers on a small and large scale as a whole increased, namely 94.91% on a small scale and 99.22% on a large scale with very good interpretation, and assessment by students as a whole also increased, namely 82% on a small scale and 83.50% on a large scale with good interpretation. It can be concluded that the e-module developed is very good and feasible to use as teaching material in the classroom and the students' independent learning process.
DETERMINATION OF OPTIMAL ANNEALING TEMPERATURE Vibrio alginolyticus PRIMERS USING POLYMERASE CHAIN REACTION METHOD Putri, Gladys Indira; Nurjayadi, Muktiningsih; Declan, Jefferson Lynford; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Fahriza, Tiara; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Saamia4, Vira; Saputro, Dwi Anna Oktaviani; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2976

Abstract

Food poisoning is a global issue of grave concern. If food is not properly cooked, it can be a medium for the spread of pathogenic bacteria. Vibrio alginolyticus is one of the pathogenic bacteria that can cause food poisoning. real-time Polymerase Chain Reaction (rt-PCR) can detect pathogenic bacteria in food, so it is necessary to determine the optimal annealing temperature. This research aims to obtain the optimal annealing temperature of the Va_Chr1_FR primer using Gradient PCR. The DNA concentration used was 174.5 with an A260/A280 purity of 1.94. The temperature range tested, 53°C-62°C, corresponds to the melting temperature of the Va_Chr1_FR primers. The primers designed were F5'-TTCTTCTGTTGTAGGTTCCG-F3' and R5'-CCAGCCCTCACATCTAATAC-R3'. Based on these results, a temperature of 60°C is deemed as the most optimal annealing temperature because it produces one of the brightest bands on electrophoresis with an amplicon length of 146 bp. The findings of this study will be beneficial to the development of Va_Chr1_FR Vibrio alginolyticus primers testing on food samples using the real-time PCR method. 
Student Preferences in Choosing Chemistry as an Elective Course in Phase F of the Merdeka Curriculum Wella, Wella Aulia Putri; Andika, Andika Prasetya; Desi, Desi Handayani; Muktiningsih, Muktiningsih; Ridwan, Achmad
Jurnal Pijar Mipa Vol. 19 No. 1 (2024): January 2024
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v19i1.6386

Abstract

The Merdeka Curriculum differs from the 2013 curriculum regarding elective subjects. The Merdeka Curriculum has no more specialization groups such as science, social studies or language. Merdeka Curriculum allows students to choose elective subjects that suit their interests, talents and abilities when entering phase F at the Senior High School level. Schools must open at least seven elective subjects adjusted to the characteristics and human resources owned by the school. Chemistry is one of the elective subjects that students can choose in Phase F of the Merdeka Curriculum. This study aims to determine the factors that make students choose chemistry as their elective subject in Phase F of the Merdeka Curriculum. This research was conducted at SMA Negeri 17 Jakarta in the 2023-2024 academic year. The research subjects were students in chemistry specialization classes: XI-A Chemistry and XI-B Chemistry. This research uses a qualitative method with a case study research type. Sampling in this study used a purposive sampling technique with certain considerations. Sampling in this study used a purposive sampling technique with certain considerations. Data collection techniques were carried out through interviews with nine students in chemistry specialization classes, and the data obtained were analyzed qualitatively with stages, namely data reduction, conclusions, and verification. The results showed that intrinsic and extrinsic factors influence students' tendency to choose chemistry as their chosen subject. Intrinsic factors include interest in chemistry, academic value, and future study or career plans. Intrinsic factors influencing students most are future study or career plans and individual interest in chemistry. In contrast, extrinsic factors include family factors, friend factors, teacher factors, and support from schools. The extrinsic factor that most influences students is the teacher factor.
Analysis of Technological Pedagogical Content Knowledge (TPACK) Ability for Prospective Chemistry Teacher Students and Chemistry Teachers: A Literature Review Raihanah, Dinnah; Putri, Nabilah Mustika; Fatmawati, Tri Kurnia; Nurjayadi, Muktiningsih
Jurnal Pijar Mipa Vol. 19 No. 1 (2024): January 2024
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v19i1.6395

Abstract

Mastering ICT is a prerequisite for teachers in the 21st century, and this is consistent with the TPACK framework that a teacher or aspiring teacher needs to be equipped with. Technological, pedagogical, and content knowledge (TPACK) is a conceptual framework that combines components that include three types of knowledge that teachers need to master, namely technological, pedagogical, and content knowledge. TPACK is a framework that seeks understanding the relationship between pedagogical and technological knowledge. In TPACK, teachers' knowledge of integrating technology into learning makes learning effective and efficient. This study aimed to observe the Technological Pedagogical Content Knowledge (TPACK) ability of prospective chemistry teachers and chemistry teachers. The research method used a literature study in the form of an analysis of various literature derived from national and international articles in accordance with the research objectives, namely 33 articles on the ability of Technological Pedagogical Content Knowledge (TPACK) of prospective chemistry teachers and chemistry teachers. The results showed that the components of TK, PK, TPK, TCK, and TPACK owned by prospective chemistry teachers and chemistry teachers were in the sufficient category. The TPACK abilities of chemistry teacher candidates and chemistry teachers still need to be improved in several aspects of content mastery, pedagogy, and technology. Developing TPACK abilities requires a long process to acquire new sources of skills and knowledge needed to form professional teachers.
Analysis of Educator and Education Personnel Standards in Indonesia Ramadhan, Fitri Nur; Lanasari, Rossy; Cahyani, Septia Dwi; Nurjayadi, Muktiningsih; Ridwan, Achmad
Jurnal Inovasi Pendidikan MH Thamrin Vol. 9 No. 1 (2025): Jurnal Inovasi Pendidikan MH Thamrin
Publisher : LPPM Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jipmht.v9i1.2541

Abstract

Education is an absolute necessity for everyone to have high competitiveness, especially in the current era of globalization, without having adequate competitiveness, it is difficult for a person to face increasingly competitive labor competition. The aim of this research is to determine the functions and problems of standardization of educators and education personnel in Indonesia. The method used in this research is qualitative library research, namely a type of research that refers to literary treasures such as book literature, laws, government regulations, articles and e-journals. The research results show that the function of standardizing educators and education personnel is: increasing professionalism, improving the quality of education, recognition and certification. Problems in implementing standards for educators and educational staff are: limited flexibility, lack of facilities and training for educators in the regions, heavy administrative burden. Solutions: flexibility in standards, improved educational infrastructure and facilities, increased access to training and certification, reduced administrative burden.
PERSPEKTIF GURU SEKOLAH MENENGAH ATAS TERHADAP PROJEK PENGUATAN PROFIL PELAJAR PANCASILA PADA KURIKULUM MERDEKA Masturdin, Masturdin; Amalia, Ira Nur; Sulastri, Sindy Kania; Nurjayadi, Muktiningsih
Jurnal Cahaya Mandalika ISSN 2721-4796 (online) Vol. 3 No. 2 (2022)
Publisher : Institut Penelitian Dan Pengambangan Mandalika Indonesia (IP2MI)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pergantian kurikulum di Indonesia adalah proses yang terus berkembang seiring waktu. Kurikulum menjadi dinamis untuk disesuaikan dengan perkembangan dan tuntutan kehidupan yang terus berubah. Projek penguatan profil pelajar pancasila merupakan salah satu bagian dari struktur kurikulum merdeka sehingga guru harus memiliki perspektif yang baik terhadap pemahaman kurikulum merdeka. Penelitian ini bertujuan untuk mengetahui perspektif guru sekolah mengah atas terhadap projek penguatan profil pelajar pancasila. Metode penelitian yang digunakan yaitu metode kualitatif, melalui metode kualitatif dapat digambarkan secara mendalam mengenai perspektif guru terahap projek penguatan profil pelajar pancasila. Berdasarkan penelitian yang telah dilakukan guru sekolah menengah atas memiliki perspektif yang baik terhadap kegiatan projek penguatan profil pelajar pancasila. Mulai dari konsep dasar, tahap persiapan, tahap pelaksanaan sampai dengan tahap asesmen dan evaluasi projek penguatan pelajaran pancasila. Hal tersebut didukung berdasarkan data hasil wawancara yang menujukan bahwa jawaban dari setiap responden sesuai dengan panduan projek penguatan pelajar pancasila yang telah di keluarkan oleh Kemendikbud, sehingga dapat di simpulkan bahwa guru sekolah menegah atas yang berada di daerah kabupaten dan kota serang provinsi Banten memiliki perspektif yang baik terhadap kegiatan projek penguatan pelajar pancasila pada kurikulum merdeka.
Co-Authors Abdul Hakam Barok Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Achmad Ridwan, Achmad Ade Danova Adinda Myra Amalia Putri Adinda Myra Amalia Putri Agung Purwanto AGUS SETIAWAN Agus Setiawan Ahmad Yudianto Aina Q Marof Akbar, Dandy Alin Mardiah Aliyah, Elvira Zulfah Amalia, Ira Nur Ananda Indah Putri Sihombing Andika, Andika Prasetya Angelina, Helzi Anggraeni, Rosita Gio Anisa Fitriyanti Annisa Auliya Annisa Maharani Annisa, Cindy Ermintia Aqilah, Amadita Shafa Arief Rahmad Maulana Akbar Arieztania Rahmadhani Arif Rahman Asri Sulfianti Athiyah Layla Atikah Nur Rahmawati Atin Supiyani Aulia Siti Pathoni Aulia Widowati Axel Mareta Ayu Berkahingrum Azidi Irwan Azizah, Puan Aqila Azzahra, Maharanianska Bassam Abomoelak Bassam Abomoelak Berkahingrum, Ayu Cahyani, Septia Dwi Chen, Shyi-Tien Dahlia Dahlia Dalia Sukmawati Dandy Akbar Juliansyah Dandy Akbar Juliansyah Dandy Akbar Juliansyah Daniel Joe Dailin Danova, Ade Darwis, Darsef Desi Lestari Desi, Desi Handayani Dessy Natalia Devi Aliefiyardi Dian Larasati Diana Vivanti Diat Nurhidayat Dudi Hardianto Dwi Ana Oktaviani El Enshasy, Hesham El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Elsa Septiani Enshasy, Hesham Ali El Erdawati Ezzaty NM Razif Fahriza, Tiara Farid Agouillal Fathya Putri Fajriani Fatmawati, Tri Kurnia Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurniadewi Fera Kurniadewi Fera Kurniadewi Fernita Puspasari Fitri W Fitriyanti, Anisa Furqoni, Abdul Hadi Geta Putri Mentari Giri Alviyanto Gladys Indira Putri Gladys Indira Putri Gladys Indira Putri Grace Grace, Grace Gusti Angieta Putri Hairishah, Nazrisya Helzi Angelina Hermawati, Elvira Hesham A. El Enshasy Hesham Ali Al-Enshashy Hesham Ali El Enshasy Hesham Ali El-Enshasy I Made Wiranatha I Made Wiranatha I Nyoman Ruja IMAM SANTOSO Imanuelle Orchidea R P Irma Ratna Kartika Irvan Maulana Irvan Maulana Irwan Saputra Isfasona Bunga Falana Ismaya Krisdawati Ismaya Krisdawati Ismaya Krisdawati Izzatul Ilma Chairinnisa Jefferson Lynford Declan Johanes Erwin Tantaruna Juliansyah, Dandy Akbar Kartika, Irma Ratna Kinanti Istantia Chantika Krisdawati, Ismaya Kurnia Agustini Kurnia Agustini Kurniadewi, Fera Lanasari, Rossy Lilis Septyarini Lim J. R Listya Ayu Saraswati Maharani Azka Azzahra Maharanianska Azzahra Maria Paristiowati Masturdin, Masturdin Maulana, Irvan Mellyna Fitriani Musie, Royna Rahma Nabilla Anisa Putrie Nasywa Fhelia Salta Nida Nur Afifah Nita Aresanti Novi Permanasari Novi Wulandari Novianti Mantasha NOVITASARI Novitasari Novitasari Oni Soesanto Soesanto Pardiana Permatasari, Nur Azmi Puan Aqila Azizah Putri Novitasari Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Putri, Nabilah Mustika Raden Haryo Bimo Setiarto Rahma, Wanda Amelia Rahmat Yunus Yunus Rahmawati, Atikah Nur Raihanah, Dinnah Ramadhan, Fitri Nur Rara Seruni Rara Seruni Retno Dwi Cahayningrum Rheva Amadea Rina Agustina Ririn Gustini Rizkahana Syehfia Rizki Fitria Roshanida A. Rahman Rosi Nurhujaimah Rosita Gio Anggraeni Royna Rahma Musie S, Restu.N S Saamia, Vira Saamia4, Vira Salsabila, Nadia Saputro, Dwi Anna Oktaviani Sara E Gomaa Sarah Adilisa Kartini Sari, Ely Puspita Setia Budi Shangkara, Muhammad Arkent Sheila Nurul Fadillah Octaviany Shyi-Tien Chen Sihombing, Ananda Indah Putri Sintia Mardita Siti Azizah Siti Fatimah SITI FATIMAH Siti Munawaoh Siti Munawaroh Siti Namiran Hadis Sondang N. Sihombing Sri Rahayu SRI RAHAYU Sulastri, Sindy Kania Sulistiyani Susilo, Ahmad Dwi Susilo, Tanto Budi Syamsul Bachri Tiara Fahriza Toton Sutono Tri Hastuti Budi Utami Tri Susanti Ucu Cahyana Ulfah Choiriyah Uswatul Nisa Vira Saamia Vira Saamia Vira Saamia Wahyuningtiyas, Kartika Putri Wella, Wella Aulia Putri Wiranatha, I Made Yohana Caroline Sihombing Yohannes Eka Cordias Buulolo Yuli Rahmawati Zahroni, Siti Zarani M Taher