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All Journal HAYATI Journal of Biosciences VALENSI Microbiology Indonesia Tadris: Jurnal keguruan dan Ilmu Tarbiyah Medical Journal of Indonesia INDONESIAN JOURNAL OF PHARMACY Jurnal Tadris Kimiya Lantanida Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal SOLMA Indonesian Journal of Chemistry SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Acta Biochimica Indonesiana Jurnal Riset Pendidikan Kimia (JRPK) Sarwahita : Jurnal Pengabdian Kepada Masyarakat Jurnal Lentera Pendidikan Pusat Penelitian LPPM UM Metro Jurnal Riset Sains dan Kimia Terapan JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Inovasi Pendidikan MH Thamrin Jurnal Cahaya Mandalika Jurnal Kajian Pembelajaran dan Keilmuan Jurnal Pijar MIPA Jurnal Pengabdian Ilung (Inovasi Lahan Basah Unggul) Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Makara Journal of Science Jurnal Green Growth dan Manajemen Lingkungan Mitra Pilar: Jurnal Pendidikan, Inovasi, dan Terapan Teknologi Journal of Innovation and Teacher Professionalism Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Kesehatan Masyarakat Jurnal Bioteknologi & Biosains Indonesia
Muktiningsih Nurjayadi
Department Of Chemistry, Faculty Of Mathematics And Natural Science, Universitas Negeri Jakarta, Gedung KH. Hasjim Asj'ari, 6th Floor, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia; Research Center For Detection Of Pathogenic Bacteria, Lembaga
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DETEKSI BAKTERI PENYEBAB PENYAKIT TYPHUS PADA MANUSIA DENGAN POLYMERASE CHAIN REACTION Muktiningsih Nurjayadi; Fera Kurnia Dewi; Dahlia Dahlia; S, Restu.N S; Fitri W
Jurnal Riset Sains dan Kimia Terapan Vol 1 No 1 (2011): JRSKT - Jurnal Riset Sains dan Kimia Terapan, Volume 1 Nomor 1 Juni 2011
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.944 KB) | DOI: 10.21009/JRSKT.011.08

Abstract

Salmonella typhi is bacteria that cause typhoid disease in humans. In Indonesia, the morbidity number of typhoid disease tends to be increase. Thus, it has been requiring the alternative for handling or preventing that disease. Recently, the detection method commonly uses for S. typhi detection is Serological test. The weakness of this method is often producing less accurate and not specific detection. The previous research was successfully discovered S. typhi gene that codes protein which is contributed at adherents or colonization those bacteria in epithelial human cell. That result was base to develop detection on S. typhi method by Polymerase chain reaction (PCR). The aim of this research is developing a specific and accurate detection method for S. typhi bacteria by PCR. The research result is performed successfully to amplify the fimbrial-C S. typhi gene using pairs of primer FW-INT 2- REV-1A NEW which was designed and synthesized in previous step. That success showed by the finding of the DNA fragment of 0.2 kilobase (kb) proffers to size of DNA fragment which is hopefully in using S. typhi genome as a template. Specificity and sensitivity test for those primers are still conducting to reproducibility results. Base on the results can be concluded that the research have successfully conducted in developing S. typhi detection method using pairs of S. typhi fimbrial-C primer. Hopefully, the studied of developing detection methods was conducted better compare with former detection methods.Keywords: S. typhi detection method, fim-C S. typhi gene, PCRAbstrakSalmonella typhi merupakan bakteri penyebab penyakit tifus pada manusia. Di Indonesia, angka morbiditas penderita penyakit typhus cenderung meningkat, sehingga diperlukan suatu alternatif untuk penanganan atau pencegahan penyakit tersebut. Sampai saat ini metode deteksi S. typhi yang banyak digunakan adalah uji serologi. Kelemahan metode ini adalah sering menghasilkan deteksi yang kurang akurat dan tidak spesifik. Pada penelitian yang dilakukan sebelumnya, telah berhasil ditemukan gen fimbrial-C S. typhi pengkode protein yang berperan dalam penempelan S. typhi pada usus manusia, hasil ini dijadikan landasan untuk pengembangan metode deteksi menggunakan teknik PCR. Tujuan penelitian ini mengembangkan metode deteksi yang akurat dan spesifik untuk bakteri penyebab penyakit typhus pada manusia. Hasil penelitian menunjukkan bahwa telah berhasil dilakukan amplifikasi gen fimbrial-C S. typhi menggunakan pasangan primer hasil perancangan yaitu FW-INT 2- REV-1A NEW. Keberhasilan tersebut ditunjukkan dengan diperolehnya pita DNA berukuran 0.2 kilo basa (kb) sesuai dengan ukuran pita DNA yang diharapkan dengan menggunakan template DNA genom bakteri S. typhi. Uji sensitivitas dan spesifisitas terhadap primer hasil rancangan sedang di kaji lebih lanjut untuk memperoleh reprodusibiltas hasil pengujian. Berdasarkan hasil yang diperoleh dapat disimpulkan bahwa telah berhasil dilakukan pengembangan metode deteksi S. typhi menggunakan pasangan primer fimbrial-C S. typhi. Pengkajian pengembangan metode deteksi yang dihasilkan ini diharapkan dapat lebih baik dibanding beberapa metode deteksi yang sudah ada.Kata Kunci: Metode Deteksi Bakteri typhus, fim-C S. typhi, PCR
Pengaruh Adjuvant Alumina dan Protein Rekombinan Fim-C Inclusion Bodies Salmonella Typhimurium terhadap Respon Imun Mencit Balb/C Dian Larasati; Muktiningsih Nurjayadi; Kurnia Agustini
Jurnal Riset Sains dan Kimia Terapan Vol 7 No 1 (2017): JRSKT - Jurnal Riset Sains dan Kimia Terapan, Volume 7 Nomor 1 Juli 2017
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (844.907 KB) | DOI: 10.21009/JRSKT.071.01

Abstract

Typhoid fever is one of the most public health problem in the world, with approximately 17 million cases of typhoid fever worldwide and 600,000 death cases each year. One method to prevent typhoid fever is vaccination. Nowadays, frequently, vaccine for typhoid fever is Ty 21a and vi polysaccharide vaccines. In other hands, it has side effect of nausea, fever, and headache. So it is still necessary to search other typhoid vaccine with minimum side effects. The purpose of this study was to obtain immunogenicity test information of recombinant protein Fim-C S. typhimurium and adjuvant alumina in Balb / C mice. The used doses were 20 μg, 40 μg, and 60 μg. Plasma was obtain then analyzed by ELISA, western immunobloting, and dot blot. Immune response of Balb / C mice from KS 1 showed significant differences antibody when dose injection 40μg / 20g BB and KS2 showed significant differences antibody when dose injection 20μg / 20g BB compared with KN. Adjuvant alumina was added to the Fim-C protein inclusion bodies Salmonella typhimurium provides a higher immune response compared to the Fim-C inclusions bodies Salmonella typhimurium protein without the addition of adjuvant alumina. The results of analysis by using western blot and dot blot showed the formation of brown color indicating the interaction between protein Fim-C inclusion bodies Salmonella typhimurium and anti-Fim-C S. typhimurium antibodies. The results of adjuvant alumina and protein Fim-C inclusion bodies of Salmonella typhimurium in Balb / C mice concluded that adjuvant alumina and Fim-C protein inclusion bodies Salmonella typhimurium provide significant increased immune response in Balb / C mice. Keyword: Salmonella typhimurium, typhoid vaccine, recombinant protein Fim-inclusion Bodies S. typhimurium, Balb/C mice
HUBUNGAN KOMITMEN DAN TANGGUNG JAWAB LINGKUNGAN TERHADAP KINERJA PENGELOLAAN HUTAN PADA PROGRAM REBOISASI Abdul Hakam Barok; Muktiningsih Muktiningsih; Diana Vivanti
Jurnal Green Growth dan Manajemen Lingkungan Vol 7 No 2 (2018): Jurnal Green Growth dan Manajemen Lingkungan
Publisher : Program Studi Manajemen Lingkungan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.964 KB) | DOI: 10.21009/jgg.072.01

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui hubungan komitmen dan tanggung jawab lingkungan terhadap kinerja pengelolaan hutan pada program reboisasi. Lokasi penelitian dilaksanakan di Resot Pemangku Hutan (RPH) Solokuro – Lamongan, dengan obyek penelitian adalah masyarakat desa Solokuro peserta program reboisasi. Pengumpulan data dilakukan dengan membagikan kuesioner kepada 75 responden sampel. Data yang terkumpul dianalisis dengan menggunakan statistik parametrik yaitu statistik deskriptif dan statistik inferensial. Pengujian hipotesis dilakukan melalui uji korelasional. Uji analisis dalam penelitian ini meliputi: (1) uji multiple (2) uji koefisien korelasi, dan (3) uji koefisien determinasi. Hasil penelitian ini menunjukkan, (1) komitmen yang tinggi berhubungan kinerja pengelolaan hutan yang tinggi, (2) tanggung jawab lingkungan yang tinggi berhubungan dengan kinerja pengelolaan hutan yang tinggi, (3) komitmen dan tanggung jawab lingkungan yang tinggi membuat kinerja pengelolaan hutan tinggi. Berdasarkan hasil penelitian tersebut bahwa komitmen dan tanggung jawab lingkungan baik sendiri-sendiri maupun bersamaan memiliki hubungan positif terhadap peningkatan kinerja pengelolaan hutan, sehingga model ini dapat dilanjutkan dan juga bisa diterapkan program reboisasi di lokasi yang berbeda.
Perakitan Gas Chromatography Sederhana Untuk Pembelajaran Instrumen Pemisahan Senyawa Kimia Tanto Budi Susilo; Rahmat Yunus Yunus; Azidi Irwan Irwan; Oni Soesanto Soesanto; Arief Rahmad Maulana Akbar; Rizki Fitria Fitria; Muktiningsih Muktiningsih Muktiningsih
Jurnal Pengabdian ILUNG (Inovasi Lahan Basah Unggul) Vol 2, No 4 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/ilung.v2i4.7682

Abstract

AbstrakGas Chromatography (GC) merupakan suatu metode standar dalam kurikulum pendidikan sains kimia. Metode ini berupa alat yang mampu memisahkan dan menganalisis senyawa multi komponen berdasarkan data fisiknya. Biasanya ukuran alat adalah besar dengan harga yang mahal. Namun, alat ini dapat dirangkai secara sederhana dengan menggunakan bahan-bahan disekitar (https://youtu.be/w9OMFAAPV0I). Metodologi perakitan mengunakan kompresor, pipa kapiler, elemen panas dan detektor alkohol. Bahan uji berupa fermentasi buah-buahan dan dipraktekkan kepada mahasiswa semester 5 Program Studi Kimia, Universitas Lambung Mangkurat. Metode Structural Equation Modelling (SEM) digunakan untuk mengetahui persepsi mahasiswa terhadap alat GC. Sebelumnya mahasiswa menganggap alat adalah GC rumit (74.4%), mahal (51.2%), tidak bisa dirakit sendiri (39.5%), berukuran besar (51.2%). Namun, setelah terlibat dalam kegiatan perakitan alat GC, persepsi mahasiswa terhadap alat tersebut berubah yaitu rumit (23.3%), mahal (11.6%), tidak bisa dirakit sendiri (0%), berukuran besar (16.3%), simpel (44.2%), biaya terjangkau (37.2%), bisa dirakit sendiri (65.1%) serta berukuran kecil (37.2%). Sehingga alat GC sederhana ini dapat menjadi alternatif pilihan ditengah mahalnya alat GC pabrikan. Kata kunci: gas chromatography; perakitan; pemisaha
Rapid Detection of Foodborne Pathogen Bacteria Vibrio parahaemolyticus in Seafood Using Gene ToxR with Real-Time Polymerase Chain Reaction Method Ismaya Krisdawati; Muktiningsih Nurjayadi; Jefferson Lynford Declan; Gladys Indira Putri; Dandy Akbar Juliansyah; Maharani Azka Azzahra; Irvan Maulana; Irma Ratna Kartika; Vira Saamia; Dwi Ana Oktaviani; I Made Wiranatha; Hesham Ali Al-Enshashy
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1613.88 KB)

Abstract

Cases of food poisoning often occur due to food contamination caused by pathogenic bacteria. One of the pathogenic bacteria is Vibrio parahaemolyticus which is found in seafood. Thus, a fast, accurate and specific detection method is needed. The purpose of this study was to quickly detect Vibrio parahaemolyticus bacteria in seafood samples targeting the ToxR gene using Real Time PCR. In a previous study, gradient PCR was used to optimize ideal annealing temperature ranges from 53-62°C and revealed that 58°C produced the best outcomes for the ToxR primer with a size of 171 base pairs. Real-Time PCR was utilized to amplify, specify, and test for sensitivity under the ideal conditions from the PCR Gradient. The confirmation results show that the primer pairs could amplify ToxR of Vibrio parahaemolyticus with the amount of concentration as much as 50 ng/µL with Ct 10,69 and 10,32 and melting curve at temperature 82,18°C and 82,23°C. This primer pair can also distinguish non-target bacteria with different Ct and melting curve temperature. The sensitivity assay for this primer can amplify DNA templates at concentration 0,0032 ng/µL. Shrimp samples that are contaminated artificially can still be detected at Ct 13,02 and Ct 13,09. Based on these results, it can be concluded that Real Time PCR with ToxR primer can be applied to develop a detection kit for Vibrio parahaemolyticus in seafood.
Rapid Detection of Foodborne Pathogen Bacteria Vibrio parahaemolyticus in Seafood using Gene ToxR with Real-Time Polymerase Chain Reaction Method Ismaya Krisdawati; Muktiningsih Nurjayadi; Jefferson Lynford Decan
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.1.15-23

Abstract

Cases of food poisoning often occur due to food contamination caused by pathogenic bacteria. One of the pathogenic bacteria is Vibrio parahaemolyticus which is found in seafood. Thus, a fast, accurate and specific detection method is needed. The purpose of this study was to quickly detect Vibrio parahaemolyticus bacteria in seafood samples targeting the ToxR gene using Real Time PCR. In a previous study, gradient PCR was used to optimize ideal annealing temperature ranges from 53-62°C and revealed that 58°C produced the best outcomes for the ToxR primer with a size of 171 base pairs. Real-Time PCR was utilized to amplify, specify, and test for sensitivity under the ideal conditions from the PCR Gradient. The confirmation results show that the primer pairs could amplify ToxR of Vibrio parahaemolyticus with the amount of concentration as much as 50 ng ?L-1 with Ct 10.69 and 10.32 and melting curve at temperature 82.18°C and 82.23°C. This primer pair can also distinguish non- target bacteria with different Ct and melting curve temperature. The sensitivity assay for this primer can amplify DNA templates at concentration 0.0032 ng ?L-1. Shrimp samples that are contaminated artificially can still be detected at Ct 13.02 and Ct 13.09. Based on these results, it can be concluded that Real Time PCR with ToxR primer can be applied to develop a detection kit for Vibrio parahaemolyticus in seafood.
Isolation and Screening Microorganisms from Black Soldier Fly Larvae (Hermetia illucens) that Producing Amylase, Protease and Cellulase Dalia Sukmawati; Rheva Amadea; Putri Novitasari; Yohana Caroline Sihombing; Axel Mareta; Yohannes Eka Cordias Buulolo; Atin Supiyani; Muktiningsih Nurdjayadi; Diat Nurhidayat; Roshanida A. Rahman; Sulistiyani; Raden Haryo Bimo Setiarto
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4516

Abstract

The Black Soldier Fly (BSF) has gained significant attention for its ability to decompose various types of organic waste and produce valuable enzymes related to its digestive system. These enzymes hold immense potential for applications across diverse industrial sectors, with promise in the livestock industry. This study aimed to extract and assess amylase, protease, and cellulase enzymes from microorganisms present in BSF larvae fermentation. The isolation technique employed was the spread plate method, followed by rigorous screening for the presence of these enzymes. The results showed 46 isolates of 26 bacterial isolates and 20 yeast isolates. In the amylase enzyme screening, a substantial 25 bacterial and 19 yeast isolates exhibited positive amylase activity. For cellulase, 20 bacterial and 14 yeast isolates displayed positive results. In the case of protease, 16 bacterial and 12 yeast isolates demonstrated protease enzyme activity. Notably, nine isolates exhibited the remarkable capability to produce multiple enzymes, including eight bacterial and one yeast isolate. These results showcase the rich enzymatic potential of BSF-associated microorganisms, offering exciting prospects for their application in various industrial sectors, especially in enhancing the efficiency and sustainability of livestock production
Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction Muktiningsih Nurjayadi; Gladys Indira Putri; Jefferson Lynford Declan; Ismaya Krisdawati; Dandy Akbar Juliansyah; Maharanianska Azzahra; Irvan Maulana; Irma Ratna Kartika; Fera Kurniadewi; Tiara Fahriza; Adinda Myra Amalia Putri; Ayu Berkahingrum; Atikah Nur Rahmawati; Rosita Gio Anggraeni; Dalia Sukmawati; Sri Rahayu; Vira Saamia; I Made Wiranatha; Bassam Abomoelak; Hesham Ali El-Enshasy
Acta Biochimica Indonesiana Vol. 7 No. 1 (2024): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.159

Abstract

Background: Seafood is highly nutritious but poses health risks when contaminated with pathogenic bacteria like Vibrio alginolyticus, which causes food poisoning and can infect marine animals and humans. Objective: This research aimed to determine the sensitivity and specificity of real-time polymerase chain reaction (rt-PCR) using the flgL primer pair to detect V. alginolyticus bacteria in seafood. Methods: The rt-PCR method was used to detect V. alginolyticus quickly, specifically, and sensitively. The flgL primer pair was evaluated for amplicon length, Ct value, Tm value, and its ability to differentiate between target and non-target bacteria. In this research, the samples tested were red snapper and blood clams. Results: The flgL primer produced an amplicon length of 224 bp. At 50 ng concentration, it yielded a Ct value of approximately 11.00 and a Tm of approximately 83°C. The flgL primer successfully differentiated between target and non-target bacteria. In sensitivity tests, it detected V. alginolyticus at concentrations as low as 1.86 x 10-3 ng/µL. Detection in seafood samples was also successful. Conclusion: The rt-PCR assay using the flgL primer pair effectively detects Vibrio alginolyticus in seafood with high specificity, sensitivity, and rapidity. These findings support its use for rapid and accurate detection of pathogenic bacteria in seafood.
Desiminasi Pengembangan Media Pembelajaran Modul Elektronik Kimia Generasi ke-4 pada Guru Kimia di Wilayah Jakarta Timur 2 Nurjayadi, Muktiningsih; Irma Ratna Kartika; Irwan Saputra; Siti Fatimah; Ririn Gustini; Uswatul Nisa; Rizkahana Syehfia; Nida Nur Afifah; Jefferson Lynford Declan; Pardiana; Sintia Mardita
Bahasa Indonesia Vol 21 No 01 (2024): Sarwahita : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/sarwahita.211.5

Abstract

generation electronic module is one of the chemistry learning media that can facilitate a three-way interaction between students, teachers and learning resources in 5.0 society era. Various chemical materials can be presented in the form of electronic modules equipped with applications according to the characteristics of the material. However, these developments are often not followed by the development of teacher competence in the field. So that there is a gap between scientific developments in Higher Education and conditions in the field. In this community service program, dissemination of the development of the 4th generation chemistry e-module has been carried out for MGMP-2 (Musyawarah Guru Mata Pelajaran) teachers in the East Jakarta Region through the dissemination of research results conducted in the Chemistry Education Program of FMIPA UNJ, that carried out face to face, starting with the delivery of material, Workshop, interactive discussion and coaching online blended learning. During the process of delivering the material, the participants enthusiastically followed it step by step. At the end of the session an evaluation questionnaire was given, the input and suggestions obtained were very positive. So, it is hoped that this activity can continue to be carried out and be useful in disseminating science and technology, increasing teacher competence and adding development information in tertiary institutions according to field needs. Abstrak Perkembangan teknologi sangat mempengaruhi perkembangan media pembelajaran kimia saat ini. Elektronik modul generasi ke-4 merupakan salah satu media pembelajaran kimia yang dapat memfasilitasi interaksi tiga arah antara siswa, guru dan sumber belajar di era society 5.0. Berbagai materi kimia dapat disajikan dalam bentuk modul elektronik yang dilengkapi dengan aplikasi sesuai karakteristik materinya. Namun perkembangan media pembelajaran tersebut sering tidak diikuti dengan perkembangan kompetensi guru di lapangan. Sehingga terjadi kesenjangan antara perkembangan keilmuan di Perguruan Tinggi dengan kondisi dilapangan. Pada program pengabdian pada masyakarat ini, telah dilakukan desiminasi pengembangan e-modul kimia generasi ke-4 bagi guru MGMP-2 (Musyawarah Guru Mata Pelajaran) Wilayah Jakarta Timur melalui desiminiasi hasil penelitian yang dilakukan di rumpun Kimia FMIPA UNJ yang dilakukan seacara tatap muka, dimulai dengan penyampaian materi, Workshop, diskusi interaktif dan pembinaan secara blanded learning. Selama proses penyampaian materi, peserta antusias mengikuti tahap demi tahap. Pada akhir sesi diberikan angket evaluasi, masukan dan saran yang didapat sangat positif. Sehingga diharapkan kegiatan ini dapat terus dilakuakan dan bermanfaat dalam menyebarluaskan IPTEK, meningkatkan kompetensi guru serta menambah informasi pengembangan di Perguruan Tinggi sesuai dengan kebutuhan dilapangan.
Development of Bacillus subtilis Detection Method Targeting Genes codY, narH, and ureC Using Polymerase Chain Reaction Nurjayadi, Muktiningsih; Berkahingrum, Ayu; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Anggraeni, Rosita Gio; Fahriza, Tiara; Declan, Jefferson Lynford; Putri, Gladys Indira; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; El Enshasy, Hesham
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.26

Abstract

Bacillus subtilis, a causative agent of foodborne illness in bread, cakes, cereals, and cheese leading to symptoms like diarrhea and nausea, was investigated for its role in nosocomial infections, including endocarditis, bacteremia, septicemia, and meningitis. The research focused on three genes: codY, narH, and ureC, which respectively contribute to bacterial survival through biofilm and spore formation, acid resistance, and adaptation to anaerobic conditions. The objective of this study was to evaluate the codY, narH, and ureC genes using the Gradient PCR method for Bacillus subtilis detection in bread and cheese through Real-Time PCR. During the research, bacterial growth was observed with an OD600 of 1.438 in Tryptic Soy Broth (TSB), and colonies of medium size, smooth, cream-coloured, and round shape were successfully isolated on Tryptic Soy Agar (TSA). The DNA template used had a 71.5 ng/µL concentration with an A260/280 ratio of approximately 1.830. The annealing temperature for Gradient PCR used was 53-62°C. The primers successfully amplified codY (175 bp), narH (222 bp), and ureC (153 bp) gene amplicons. The optimal annealing temperature for the primers used was 60°C, as indicated by the presence of a single bright band in electrophoresis. Using these three different genes, testing can also be conducted with the Multiplex PCR method. The next step involves developing a detection kit using optimized primers and annealing temperatures for the identification of Bacillus subtilis in bread and cheese samples using Real-Time PCR.
Co-Authors Abdul Hakam Barok Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Achmad Ridwan Achmad Ridwan, Achmad Ade Danova Adinda Myra Amalia Putri Adinda Myra Amalia Putri Agung Purwanto AGUS SETIAWAN Ahmad Yudianto Aina Q Marof Aisyafitri, Afifah Akbar, Dandy Albana, Fadhila Shofi Alin Mardiah Aliyah, Elvira Zulfah Amalia, Ira Nur Ananda Indah Putri Sihombing Andika, Andika Prasetya Angelina, Helzi Anggraeni, Rosita Gio Anisa Fitriyanti Annisa Auliya Annisa Maharani Annisa, Cindy Ermintia Aqilah, Amadita Shafa Arief Rahmad Maulana Akbar Arieztania Rahmadhani Arif Rahman Asri Sulfianti Athiyah Layla Atikah Nur Rahmawati Atin Supiyani Aulia Siti Pathoni Aulia Widowati Aurellia, Dema Griseldis Axel Mareta Ayu Berkahingrum Azahra, Nur Fadillah Adelia Azidi Irwan Azizah, Puan Aqila Azzahra, Maharanianska Bassam Abomoelak Bassam Abomoelak Berkahingrum, Ayu Cahyani, Septia Dwi Chen, Shyi-Tien Dahlia Dahlia Dalia Sukmawati Dandy Akbar Juliansyah Dandy Akbar Juliansyah Dandy Akbar Juliansyah Daniel Joe Dailin Danova, Ade Darwis, Darsef Desi Lestari Desi, Desi Handayani Dessy Natalia Devi Aliefiyardi Dian Larasati Diana Vivanti Diat Nurhidayat Dudi Hardianto Dwi Ana Oktaviani El Enshasy, Hesham El Enshasy, Hesham Ali El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Elsa Septiani Ely Puspita Sari Enshasy, Hesham Ali El Erdawati Erliasna, Emia Ezzaty NM Razif Fahriza, Tiara Farid Agouillal Fathya Putri Fajriani Fatmawati, Tri Kurnia Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurniadewi Fera Kurniadewi Fera Kurniadewi Fernita Puspasari Fitri W Fitri, Ika Keumala Fitriyanti, Anisa Furqoni, Abdul Hadi Geta Putri Mentari Giri Alviyanto Gladys Indira Putri Gladys Indira Putri Gladys Indira Putri Grace Grace, Grace Gusti Angieta Putri Hairishah, Nazrisya Hati, Futi Kusuma Helzi Angelina Hermawati, Elvira Hesham A. El Enshasy Hesham Ali Al-Enshashy Hesham Ali El Enshasy Hesham Ali El-Enshasy I Made Wiranatha I Made Wiranatha I Nyoman Ruja IMAM SANTOSO Imanuelle Orchidea R P Irma Ratna Kartika Irvan Maulana Irvan Maulana Irwan Saputra Isfasona Bunga Falana Ismaya Krisdawati Ismaya Krisdawati Ismaya Krisdawati Izzatul Ilma Chairinnisa Jefferson Lynford Declan Johanes Erwin Tantaruna Juliansyah, Dandy Akbar Kartika, Irma Ratna Kinanti Istantia Chantika Krisdawati, Ismaya Kurnia Agustini Kurnia Agustini Kurniadewi, Fera Lanasari, Rossy Lilis Septyarini Lim J. R Listya Ayu Saraswati Maharani Azka Azzahra Maharanianska Azzahra Maria Paristiowati Masturdin, Masturdin Maulana, Irvan Mellyna Fitriani Meyliana Wulandari Musie, Royna Rahma Nabilla Anisa Putrie Nasywa Fhelia Salta Nida Nur Afifah Nita Aresanti Novi Permanasari Novi Wulandari Novianti Mantasha NOVITASARI Novitasari Novitasari Nurhayati Muslimah Nurullaili Malanul Hikmah Nur’aini, Ruhul Aflah Oni Soesanto Soesanto Pardiana Permatasari, Nur Azmi Priyani, Valda Tsabitha Puan Aqila Azizah Purnama, Yaktiva Dwi Putri Novitasari Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Putri, Nabilah Mustika Raden Haryo Bimo Setiarto Rahma, Wanda Amelia Rahmat Yunus Yunus Rahmawati, Atikah Nur Raihanah, Dinnah Ramadhan, Fitri Nur Rara Seruni Rara Seruni Retno Dwi Cahayningrum Rheva Amadea Rina Agustina Ririn Gustini Rizkahana Syehfia Rizki Fitria Roshanida A. Rahman Rosi Nurhujaimah Rosita Gio Anggraeni Royna Rahma Musie S, Restu.N S Saamia, Vira Saamia4, Vira Salsabila, Nadia Saputro, Dwi Anna Oktaviani Sara E Gomaa Sarah Adilisa Kartini Sari, Ely Puspita Setia Budi Shangkara, Muhammad Arkent Sheila Nurul Fadillah Octaviany Shyi-Tien Chen Sihombing, Ananda Indah Putri Sintia Mardita Siti Azizah Siti Fatimah SITI FATIMAH Siti Munawaoh Siti Munawaroh Siti Namiran Hadis Siti Zahroni Sondang N. Sihombing Sri Rahayu SRI RAHAYU Sulastri, Sindy Kania Sulistiyani Susilo, Ahmad Dwi Susilo, Tanto Budi Syamsul Bachri Tiara Fahriza Toton Sutono Tri Hastuti Budi Utami Tri Susanti Ucu Cahyana Ulfah Choiriyah Uswatul Nisa Vira Saamia Vira Saamia Vira Saamia Wahyuningtiyas, Kartika Putri Wella, Wella Aulia Putri Wiranatha, I Made Yohana Caroline Sihombing Yohannes Eka Cordias Buulolo Yuli Rahmawati Zarani M Taher Zeral, Lauzer