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All Journal HAYATI Journal of Biosciences VALENSI Microbiology Indonesia Tadris: Jurnal keguruan dan Ilmu Tarbiyah Medical Journal of Indonesia INDONESIAN JOURNAL OF PHARMACY Jurnal Tadris Kimiya Jurnal Penelitian Pendidikan IPA (JPPIPA) PAEDAGOGIA Indonesian Journal of Chemistry SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Acta Biochimica Indonesiana JKPK (Jurnal Kimia dan Pendidikan Kimia) Jurnal Riset Pendidikan Kimia (JRPK) Sarwahita : Jurnal Pengabdian Kepada Masyarakat Jurnal Lentera Pendidikan Pusat Penelitian LPPM UM Metro Jurnal Riset Sains dan Kimia Terapan JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Inovasi Pendidikan MH Thamrin Jurnal Cahaya Mandalika Jurnal Kajian Pembelajaran dan Keilmuan Jurnal Pijar MIPA Jurnal Pengabdian Ilung (Inovasi Lahan Basah Unggul) Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Makara Journal of Science Jurnal Green Growth dan Manajemen Lingkungan Journal of Innovation and Teacher Professionalism Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Kesehatan Masyarakat Jurnal Bioteknologi & Biosains Indonesia
Muktiningsih Nurjayadi
Department Of Chemistry, Faculty Of Mathematics And Natural Science, Universitas Negeri Jakarta, Gedung KH. Hasjim Asj'ari, 6th Floor, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia; Research Center For Detection Of Pathogenic Bacteria, Lembaga
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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DISEMINASI PEMBELAJARAN STRUKTUR DAN FUNGSI BIOMOLEKUL PROTEIN BAGI GURU WILAYAH MGMP-1 JAKARTA TIMUR Nurjayadi, Muktiningsih; Saputra, Irwan; Putri, Gladys Indira; Declan, Jefferson Lynford; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Hairishah, Nazrisya
Bahasa Indonesia Vol 20 No 01 (2023): Sarwahita : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/sarwahita.201.3

Abstract

Materi biokimia di SMA dipelajari pada kelas XII dengan salah satu pembahasan mengenai biomolekul protein. Pada pandemi COVID-19, terjadi perubahan sistem pembelajaran yang membutuhkan berbagai inovasi agar pembelajaran berlangsung efektif. Program pengabdian pada masyakarat ini, melakukan pengembangan pembelajaran struktur dan fungsi biomolekul protein bagi guru MGMP Wilayah Jakarta Timur-1 melalui desiminiasi elektonik modul Struktur dan Fungsi Protein hasil penelitian sebelumnya serta praktikum berbasis bahan rumahan dengan teknik blended learning. Pelaksanaan P2M yang dilakukan bersama Tim UNJ pada Guru-guru Kimia MGMP Jakarta Timur wilayah 1 berjalan dengan lancar. Peserta sangat antusias mengikuti materi yang disampaikan dan ikut berkontribusi dalam pelaksanaan dan mengamati praktikum uji kualitatif asam amino dan protein menggunakan bahan-bahan yang ada disekitar rumah, ramah lingkungan, dan sekaligus menerapkan konsep green chemistry. Berdasarkan hasil umpan balik, 90% peserta merasa puas dengan penyampaian materi yang disampaikan, memperoleh wawasan baru, relevan dengan yang diharapkan, dapat diimplementasikan di sekolah, dan menimbulkan ide baru untuk mendesain pembelajaran yang inovatif. Sehingga diharapkan kegiatan pengabdian kepada masyarakat yang dilakukan oleh dosen Universitas Negeri Jakarta bekerjasama dengan MGMP wilayah Jakarta Timur 1 sebagai wilayah binaan yang meliputi kecamatan Cakung, Duren Sawit, Jatinegara, Matraman, dan Pulogadung, dapat bermanfaat dalam menyebarluaskan IPTEKS dan meningkatkan kompetensi guru.
STUDI DESKRIPTIF KUALITATIF : ANALISIS PERBANDINGAN ANTARA SISTEM PENDIDIKAN AMERIKA SERIKAT DAN INDONESIA Susilo, Ahmad Dwi; Annisa, Cindy Ermintia; Azizah, Siti; Nurjayadi, Muktiningsih; Ridwan, Achmad
Jurnal Kajian Pembelajaran dan Keilmuan Vol 9, No 1 (2025): APRIL 2025
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jurnalkpk.v9i1.89372

Abstract

Education is an important part of a person's life. Through education a person can gain knowledge about morals, science, character, and other things that are important for social and state life. Each country has its own standards in providing education to its citizens. The purpose of writing this article is to make a comparison between education standards in Indonesia and the United States, especially in the state of California. The research method used is literature research. This study looked at several previous research studies and the standards applied in the two countries. Data analysis was conducted using qualitative descriptive techniques. Education standards in Indonesia and the United States-California are different. This can be seen from the different standards and outcomes. In the curriculum, Indonesia has a centralized curriculum which at the basic education level consists of 10 subjects plus local content. Whereas in the United States the basic education curriculum prioritizes soft skills with 4 general subjects that are derived in the form of sub-subjects. However, both the United States-California and Indonesia try to implement the best education standards according to the capabilities of the citizens and the characteristics of each country.
Detection of the Yersinia enterocolitica Bacteria Targeting the myfA and ystA Genes in Contaminated Vegetable Samples using Real-Time PCR to Develop Rapid Detection of Food Poisoning Bacteria Nurjayadi, Muktiningsih; Anggraeni, Rosita GIo; Putri, Gladys Indira; Declan, Jefferson Lynford; Juliansyah, Dandy Akbar; Fahriza, Tiara; Putri, Adinda Myra Amalia; Berkahingrum, Ayu; Rahmawati, Atikah Nur; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.989-1002

Abstract

Yersinia enterocolitica is a pathogenic bacterium with the ability to survive and multiply in food in a low-temperature environment that can cause death in humans. In previous studies, the optimum annealing temperature of ymoA, ystA, and ail gene primers with amplicons of 185 bp, 123 bp, and 192 bp, respectively, was successfully found. This study aims to develop a pathogenic bacteria detection kit with confirmation, sensitivity, and specificity of myfA and ystA primers in detecting Yersinia enterocolitica bacteria quickly and accurately using the real-time Polymerase Chain Reaction method. The results showed that myfA and ystA primers have optimum annealing temperatures at 60°C with amplicon lengths of 181 bp and 123 bp, respectively. Primer myfA was able to amplify the target with real-time PCR at Ct 12.07±1 and Tm 81±1°C, while the ystA primer at Ct 12.38±1 and Tm 83±1°C. myfA and ystA primers were also able to distinguish target and non-target bacteria based on Ct or Tm. The designed primers successfully detected Yersinia enterocolitica bacteria with the smallest concentration of 0.000439 ng/µL equivalent to 7.024 × 102 CFU. The detection limit obtained is smaller than the contamination threshold set by the Food and Drug Administration (BPOM). Primer myfA and ystA Yersinia enterocolitica also successfully detected the target bacteria in cabbage and lettuce samples artificially. Based on these results, myfA and ystA primers successfully detected Yersinia enterocolitica in vegetable samples using real-time PCR quickly, sensitively, specifically, and accurately.
Penerapan Multimedia Pembelajaran Interaktif Berbasis Mobile Learning untuk Meningkatkan Minat dan Hasil Belajar Peserta Didik Wahyuningtiyas, Kartika Putri; Bachri, Syamsul; Muktiningsih, Muktiningsih
Journal of Innovation and Teacher Professionalism Vol. 2 No. 2 (2024)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um084v2i22024p141-149

Abstract

Untuk mengatasi kurangnya keterlibatan dan minat peserta didik dalam pembelajaran, sebuah penelitian dilakukan dengan tujuan untuk meningkatkan minat dan hasil belajar mereka menggunakan media pembelajaran berbasis ponsel. Metode penelitian yang digunakan adalah Penelitian Tindakan Kelas (PTK) dengan melibatkan 35 peserta didik dari kelas IX F di SMP Negeri 2 Malang. Data dikumpulkan melalui tes dan kuesioner, kemudian dianalisis menggunakan metode statistik deskriptif kuantitatif. Hasil penelitian menunjukkan peningkatan minat dan hasil belajar peserta didik dalam pembelajaran IPS. Terlihat dari data pra-siklus, di mana skor rata-rata meningkat dari 81,9 menjadi 90,45, dan pada data pasca-siklus, dengan skor rata-rata mencapai 70 dengan tingkat ketuntasan klasik sebesar 48,58 persen, meningkat menjadi skor rata-rata 83 dengan tingkat ketuntasan klasik sebesar 80 persen. Dengan demikian, penggunaan multimedia pembelajaran berbasis mobile learning mampu meningkatkan minat dan hasil belajar peserta didik dalam konteks pembelajaran IPS. Kata kunci: multimedia pembelajaran; mobile learning; mata pelajaran IPS; minat belajar; hasil belajar Application of Interactive Learning Multimedia Based on Mobile Learning to Increase Students' Interest and Learning Outcomes A study was conducted to address learners' lack of engagement and interest in learning. The aim was to improve their interest and learning outcomes using mobile phone-based learning media. The research method used was Classroom Action Research (PTK), involving 35 learners from class IX F at SMP Negeri 2 Malang. Data was collected through tests and questionnaires and then analyzed using quantitative descriptive statistical methods. The results showed an increase in students' interest and learning outcomes in social studies learning. The pre-cycle data revealed an average score of 81.9, which increased to 90.45 in the post-cycle data. The classic completeness rate was 48.58 percent, while the average score reached 70. In contrast, the average score increased to 83 with a classic completeness rate of 80 percent in the post-cycle data. Consequently, the utilization of mobile learning-based multimedia can enhance students' engagement and academic performance in the context of social studies education. Keywords: learning multimedia; mobile learning; social sciences subjects; interest learning; learning outcomes
Strategi Culturally Responsive Teaching dalam Pembelajaran IPS untuk Meningkatkan Kesadaran Kebinekaan Global Aliyah, Elvira Zulfah; Ruja, I Nyoman; Muktiningsih, Muktiningsih
Journal of Innovation and Teacher Professionalism Vol. 3 No. 3 (2025)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um084v3i32025p711-719

Abstract

Guru IPS kelas VIII SMPN 2 Malang menerapkan pendekatan Culturally Responsive Teaching (CRT) berbasis budaya lokal Malang Raya untuk menguatkan profil pelajar Pancasila dimensi berkebinekaan global. Penelitian ini bertujuan untuk: (1) mengetahui implementasi pendekatan CRT dalam pembelajaran IPS, dan (2) mengidentifikasi peluang serta tantangan dalam penerapannya. Penelitian ini menggunakan metode kualitatif deskriptif dengan subjek peserta didik kelas VIII F SMPN 2 Malang. Teknik pengumpulan data meliputi observasi, wawancara, dan dokumentasi. Analisis data dilakukan dengan model Miles dan Huberman, sementara validitas data diuji melalui triangulasi waktu dan metode. Hasil penelitian menunjukkan bahwa pembelajaran IPS berbasis CRT berjalan lancar dan kondusif. Terdapat tiga peluang utama dalam implementasinya: (1) keberagaman budaya Malang Raya yang dapat dieksplorasi, (2) sarana dan prasarana sekolah yang memadai, dan (3) fleksibilitas guru dalam mendesain pembelajaran sesuai latar belakang sosial-budaya peserta didik. Namun, terdapat tantangan seperti kebutuhan strategi pembelajaran yang tepat dan manajemen waktu yang efisien.
Analysis of Educator and Education Personnel Standards in Indonesia Ramadhan, Fitri Nur; Lanasari, Rossy; Cahyani, Septia Dwi; Nurjayadi, Muktiningsih; Ridwan, Achmad
Jurnal Inovasi Pendidikan MH Thamrin Vol. 9 No. 1 (2025): Jurnal Inovasi Pendidikan MH Thamrin
Publisher : LPPM Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jipmht.v9i1.2541

Abstract

Education is an absolute necessity for everyone to have high competitiveness, especially in the current era of globalization, without having adequate competitiveness, it is difficult for a person to face increasingly competitive labor competition. The aim of this research is to determine the functions and problems of standardization of educators and education personnel in Indonesia. The method used in this research is qualitative library research, namely a type of research that refers to literary treasures such as book literature, laws, government regulations, articles and e-journals. The research results show that the function of standardizing educators and education personnel is: increasing professionalism, improving the quality of education, recognition and certification. Problems in implementing standards for educators and educational staff are: limited flexibility, lack of facilities and training for educators in the regions, heavy administrative burden. Solutions: flexibility in standards, improved educational infrastructure and facilities, increased access to training and certification, reduced administrative burden.
DISEMINASI MODUL ELEKTRONIK KIMIA GENERASI KE- 4 BERBASIS ETNOSAINS DAN ECO-STEAM PADA GURU KIMIA DI MGMP WILAYAH JAKARTA SELATAN 2 Muktiningsih Nurjayadi; Fera Kurniadewi; Irma Ratna Kartika; Siti Fatimah; Nasywa Fhelia Salta; Athiyah Layla; Mellyna Fitriani; Annisa Maharani; Sarah Adilisa Kartini; Nabilla Anisa Putrie; Jefferson Lynford Declan
Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2024): PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT - SNPPM2024
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstract The rapid advancement of technology has profoundly influenced the development of learning media, accessibility, and the overall quality of education. The 4th generation electronic chemistry module represents an innovative educational tool that integrates visual, audio, audio-visual, and interactive features, facilitating enhanced communicating among learning resources, students, and educators to achieve learning objectives. This Community Service Program (P2M) aimed to disseminate research findings related to the 4th generation electronic module, which incorporates elements of ethnoscience and eco-STEAM. The dissemination activities were conducted with chemistry teachers from the South Jakarta 2 MGMP through a series of informational sessions and workshops. During the informational sessions, the theoretical framework underpinning the module's development was presented, along with an overview of the necessary applications, developmental procedures, and the integration of features such as videos, quizzes, images, pop-ups, and other interactive media tailored to specific instructional models. In the workshop component, participants were guided through the process of creating electronic modules, culminating in the successful production of a standardized prototype. The results showed that approximately 94.7% to 98.9% of participants were satisfied with the training. They found the delivery of the training material to be relevant and as expected, applicable to chemistry learning in schools, and inspiring for new interactive teaching ideas. Participants also felt that the presenter had a strong understanding of the topic, used the allotted time effectively, communicated the material clearly, answered questions well, and ensured that the training ran smoothly. Based on the findings, it is concluded that the dissemination of the 4th generation electronic chemistry module was highly effective. Keywords: The 4th generation Chemistry Electronic Module, Ethnoscience, Eco-STEAM, MGMP Abstrak Perkembangan teknologi yang semakin pesat memberikan dampak signifikan pada pengembangan media, aksesibilitas dan kualitas pembelajaran. Modul elektronik kimia generasi ke-4 merupakan Modul elektronik yang dapat menampilkan secara komprehensif, baik visual, audio, audio-visual, dan aktivitas interaktif antara sumber belajar, peserta didik dan guru untuk mencapai tujuan pembelajaran. Program Pengabdian kepada Masyarakat (P2M) ini bertujuan melakukan diseminasi produk penelitian tentang modul elektronik generasi ke-4 yang diwarnai dengan Etnosains dan Eco-STEAM. Diseminasi dilakukan pada guru-guru di MGMP Kimia wilayah Jakarta Selatan 2 dengan metode penyampaian materi scara diskusi informasi, dan workshop. Pada sesi materi disampaikan teori yang mendasari pengembangan modul, berbagai aplikasi yang dibutuhkan, langkah pengembangan, penambahan fitur video, kuis, gambar, teknik pop up, dan media interaktif lainnya yang dibuat berdasarkan alur model pembelajaran tertentu. Pada tahap workshop peserta diberikan kesempatan dan dibimbing dalam pembuatan elektronik modul sampai menghasilkan prototype standar. Kepuasan dari peserta diukur melalui kuesioner dan tanya jawab, hasilnya menunjukkan bahwa 94,7-98,9% peserta puas dengan penyampaian materi pelatihan, materi yang disampaikan relevan dan sesuai dengan yang diharapkan, dapat diterapkan dalam pembelajaran kimia di sekolah, memunculkan ide-ide baru untuk melaksanakan pembelajaran interaktif di kelas, pemateri sangat memahami topik yang disampaikan, waktu penyampaian materi mencukupi, penyampaian materi sangat baik dan mudah dipahami, pemateri menjawab pertanyaan peserta dengan sangat baik dan pelatihan berlangsung dengan lancar. Berdasarkan hasil yang diperoleh disimpulkan bahwa diseminasi modul elektronik kimia generasi ke-4 telah berhasil dengan sangat baik. Kata Kunci: Modul Elektronik Kimia generasi ke-4, Etnosains, Eco-STEAM, MGMP
Deteksi Vibrio parahaemolyticus Menggunakan Primer Gen toxR2 dengan Gradient Polymerase Chain Reaction Nurjayadi, Muktiningsih; Krisdawati, Ismaya; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Rahmawati, Atikah Nur; Fitriyanti, Anisa; Musie, Royna Rahma; Kurniadewi, Fera; Sukmawati, Dalia; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; Elenshasy, Hesham Ali
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.03

Abstract

Makanan adalah kebutuhan vital, dengan kriteria utama adalah keamanan, kualitas, dan nilai gizi. Untuk memastikan keamanan makanan, diperlukan metode deteksi yang cepat dan akurat, terutama untuk mendeteksi bakteri patogen penyebab keracunan makanan. Vibrio parahaemolyticus merupakan bakteri patogen yang banyak ditemukan pada makanan laut. Penelitian ini bertujuan untuk mengembangkan metode deteksi cepat V. parahaemolyticus dengan menargetkan gen toxR2 menggunakan Gradient Polymerase Chain Reaction. Gen toxR dipilih karena fungsinya sebagai pengatur penting gen virulensi. Tahapan yang dilakukan meliputi desain primer, penyiapan sampel bakteri dari biakan murni, dan uji amplifikasi menggunakan PCR Gradien. Hasil uji amplifikasi menunjukan bahwa pasangan primer toxR2 berhasil mengamplifikasi pada suhu 58-62°C dengan dihasilkan pita berukuran 137 bp. Berdasarkan hasil tersebut, dapat disimpulkan bahwa PCR Gradien dengan primer toxR2 dapat diaplikasikan untuk mengembangkan alat deteksi V. parahaemolyticus dengan dilakukan uji lanjutan seperti uji konfirmasi, uji spesifisitas, uji sensitivitas, dan uji pada pangan menggunakan Real-Time Polymerase Chain Reaction.
The Effect of a Small-Scale Practical Kit and a Practical Instruction Flipbook on Students’ Learning Motivation in Acid-Base Material Sari, Ely Puspita; Nurjayadi, Muktiningsih; Kurniadewi, Fera
Tadris: Jurnal Keguruan dan Ilmu Tarbiyah Vol 10 No 1 (2025): Tadris: Jurnal Keguruan dan Ilmu Tarbiyah
Publisher : Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/tadris.v10i1.24450

Abstract

This study examines the impact of small-scale practicum kits and flipbook-based instruction on students’ learning motivation in chemistry, specifically in acid–base material. Conducted at SMAN 15 Tangerang Regency, a school with limited laboratory resources, the research employed a quasi-experimental design with a pretest–posttest control group. The experimental group (Class E) utilized small-scale practicum kits and flipbooks, while the control group (Class C) used traditional large-scale laboratory equipment and student worksheets. A total of 34 students in the experimental class and 36 in the control class participated in the study. Data were analyzed using SPSS 26.0, and the statistical tests revealed a significant increase in learning motivation in the experimental group (p = 0.016). The average posttest score for Class E was 60.04, compared to 52.08 for Class C, indicating that the small-scale practicum kits and flipbooks positively influenced students’ motivation. These findings suggest that integrating sustainable and interactive learning tools not only enhances student engagement but also addresses the challenges of resource-limited educational settings. This study contributes to the growing body of literature on the efficacy of small-scale chemistry kits and digital instructional tools in fostering sustainable and inclusive learning experiences in secondary education.
Detection Method for Escherichia coli Using Real-Time Polymerase Chain Reaction Targeting the yhaV Gene Nurjayadi, Muktiningsih; Fitriyanti, Anisa; Musie, Royna Rahma; Putri, Gusti Angieta; Azizah, Puan Aqila; Angelina, Helzi; Grace, Grace; Sihombing, Ananda Indah Putri; Setiawan, Agus; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Fatimah, Siti; Berkahingrum, Ayu; Kartika, Irma Ratna; Kurniadewi, Fera; Saamia, Vira; Chen, Shyi-Tien; Aboemolak, Bassam; Enshasy, Hesham Ali El
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Escherichia coli is a foodborne pathogenic bacterium that can cause diarrhea, while yhaV is a virulence-associated gene linked to the toxin–antitoxin system in E. coli. This study was aimed at evaluating the confirmation, specificity, and sensitivity of a yhaV gene primer using real-time polymerase chain reaction. The yhaV-targeting PCR successfully amplified a DNA fragment with an amplicon length of 207 bp (base pairs) under an annealing temperature optimized to a range of 54 °C to 62 °C via gradient PCR. The PCR using the primer pair produced a consistent Ct (cycle threshold) of 14.14 ± 0.05 and showed a single peak in the melting curve at a Tm (melting temperature) of 83.67 °C ± 0.02. The specificity test indicated that the yhaV primer effectively distinguished E. coli from nontarget bacteria on the basis of differences in Ct and Tm values. The sensitivity analysis showed that the PCR directed toward the primer pair successfully detected E. coli at a minimum concentration of 2.24 pg/µL, with a Ct value of 29.93 and a detection limit of 31.5 × 102 CFU. These results suggest that yhaV-based real-time PCR quickly and accurately identifies E. coli. Primer designs that target yhaV have the potential to be developed as components of a rapid, specific, and sensitive kit for detecting E. coli in food samples.
Co-Authors Abdul Hakam Barok Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Achmad Ridwan, Achmad Ade Danova Adinda Myra Amalia Putri Adinda Myra Amalia Putri Agung Purwanto AGUS SETIAWAN Agus Setiawan Ahmad Yudianto Aina Q Marof Akbar, Dandy Alin Mardiah Aliyah, Elvira Zulfah Amalia, Ira Nur Ananda Indah Putri Sihombing Andika, Andika Prasetya Angelina, Helzi Anggraeni, Rosita Gio Anisa Fitriyanti Annisa Auliya Annisa Maharani Annisa, Cindy Ermintia Aqilah, Amadita Shafa Arief Rahmad Maulana Akbar Arieztania Rahmadhani Arif Rahman Asri Sulfianti Athiyah Layla Atikah Nur Rahmawati Atin Supiyani Aulia Siti Pathoni Aulia Widowati Axel Mareta Ayu Berkahingrum Azidi Irwan Azizah, Puan Aqila Azzahra, Maharanianska Bassam Abomoelak Bassam Abomoelak Berkahingrum, Ayu Cahyani, Septia Dwi Chen, Shyi-Tien Dahlia Dahlia Dalia Sukmawati Dandy Akbar Juliansyah Dandy Akbar Juliansyah Dandy Akbar Juliansyah Daniel Joe Dailin Danova, Ade Darwis, Darsef Desi Lestari Desi, Desi Handayani Dessy Natalia Devi Aliefiyardi Dian Larasati Diana Vivanti Diat Nurhidayat Dudi Hardianto Dwi Ana Oktaviani El Enshasy, Hesham El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Elsa Septiani Enshasy, Hesham Ali El Erdawati Ezzaty NM Razif Fahriza, Tiara Farid Agouillal Fathya Putri Fajriani Fatmawati, Tri Kurnia Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurnia Dewi Fera Kurniadewi Fera Kurniadewi Fera Kurniadewi Fernita Puspasari Fitri W Fitriyanti, Anisa Furqoni, Abdul Hadi Geta Putri Mentari Giri Alviyanto Gladys Indira Putri Gladys Indira Putri Gladys Indira Putri Grace Grace, Grace Gusti Angieta Putri Hairishah, Nazrisya Helzi Angelina Hermawati, Elvira Hesham A. El Enshasy Hesham Ali Al-Enshashy Hesham Ali El Enshasy Hesham Ali El-Enshasy I Made Wiranatha I Made Wiranatha I Nyoman Ruja IMAM SANTOSO Imanuelle Orchidea R P Irma Ratna Kartika Irvan Maulana Irvan Maulana Irwan Saputra Isfasona Bunga Falana Ismaya Krisdawati Ismaya Krisdawati Ismaya Krisdawati Izzatul Ilma Chairinnisa Jefferson Lynford Declan Johanes Erwin Tantaruna Juliansyah, Dandy Akbar Kartika, Irma Ratna Kinanti Istantia Chantika Krisdawati, Ismaya Kurnia Agustini Kurnia Agustini Kurniadewi, Fera Lanasari, Rossy Lilis Septyarini Lim J. R Listya Ayu Saraswati Maharani Azka Azzahra Maharanianska Azzahra Maria Paristiowati Masturdin, Masturdin Maulana, Irvan Mellyna Fitriani Musie, Royna Rahma Nabilla Anisa Putrie Nasywa Fhelia Salta Nida Nur Afifah Nita Aresanti Novi Permanasari Novi Wulandari Novianti Mantasha NOVITASARI Novitasari Novitasari Oni Soesanto Soesanto Pardiana Permatasari, Nur Azmi Puan Aqila Azizah Putri Novitasari Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Putri, Nabilah Mustika Raden Haryo Bimo Setiarto Rahma, Wanda Amelia Rahmat Yunus Yunus Rahmawati, Atikah Nur Raihanah, Dinnah Ramadhan, Fitri Nur Rara Seruni Rara Seruni Retno Dwi Cahayningrum Rheva Amadea Rina Agustina Ririn Gustini Rizkahana Syehfia Rizki Fitria Roshanida A. Rahman Rosi Nurhujaimah Rosita Gio Anggraeni Royna Rahma Musie S, Restu.N S Saamia, Vira Saamia4, Vira Salsabila, Nadia Saputro, Dwi Anna Oktaviani Sara E Gomaa Sarah Adilisa Kartini Sari, Ely Puspita Setia Budi Shangkara, Muhammad Arkent Sheila Nurul Fadillah Octaviany Shyi-Tien Chen Sihombing, Ananda Indah Putri Sintia Mardita Siti Azizah SITI FATIMAH Siti Fatimah Siti Munawaoh Siti Munawaroh Siti Namiran Hadis Sondang N. Sihombing Sri Rahayu SRI RAHAYU Sulastri, Sindy Kania Sulistiyani Susilo, Ahmad Dwi Susilo, Tanto Budi Syamsul Bachri Tiara Fahriza Toton Sutono Tri Hastuti Budi Utami Tri Susanti Ucu Cahyana Ulfah Choiriyah Uswatul Nisa Vira Saamia Vira Saamia Vira Saamia Wahyuningtiyas, Kartika Putri Wella, Wella Aulia Putri Wiranatha, I Made Yohana Caroline Sihombing Yohannes Eka Cordias Buulolo Yuli Rahmawati Zahroni, Siti Zarani M Taher