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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA METANA Journal of Oceanography Journal of Marine Research BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Kelautan Tropis JMM (Jurnal Masyarakat Mandiri) JFM (Jurnal Farmasi Malahayati) Journal of Tropical Marine Science Journal of Research in Social Science and Humanities J-CEKI

Widianingsih Widianingsih

Department Of Marine Science, Faculty Of Fisheries And Marine Sciences, Diponegoro University

Author-ID : 415420

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Pemangsaan Propagul Mangrove Rhizophora sp. Sebagai Bukti Teori Dominance-Predation (Predation of Mangrove Propagule, Rhizophora sp. as Evidence of Dominance-Predation Theory) Rudhi Pribadi; Achmad Muhajir; Widianingsih Widianingsih; Retno Hartati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 19, No 2 (2014): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Pemangsaan sering mentebabkan kegagalan propagul mangrove untuk tumbuh menjadi individu baru. Pemangsaan terjadi saat propagul masih bergantung pada pohon induk atau pra-penyebaran maupun setelah terlepas dan jatuh dari pohon induk atau paska-penyebaran. Penelitian ini bertujuan untuk mengetahui tingkat pemangsaan propagul Rhizophora mucronata Lamk., R. stylosa Griff. dan R. apiculata Blume di Desa Pasar Banggi, Kabupaten Rembang, Jawa Tengah. Penelitian terdiri dari 3 tahap yaitu penelitian pendahuluan untuk mengetahui dominasi spesies, penelitian deskriptif pada pra-penyebaran tentang pemangsaan propagul pra-penyebaran dan penelitian eksperimental untuk mengetahui hubungan antara dominasi spesies dengan tingkat pemangsaan propagul paska-penyebaran. Tingkat pemangsaan propagul pra-penyebaran R. stylosa terbukti paling tinggi tingkat pemangsaannya yaitu 61,06% (kisaran 45,40-76,05%), disusul R. apiculata 58,18% (47,41-68,00%) dan terendah R. mucronata 11,88% (7,06-15,71%). Selama 18 hari pengamatan tingkat pemangsaan propagul R. stylosa paling rendah di stasiun yang didominasi R. stylosa (46,67%) dibandingkan di stasiun yang di dominasi R. apiculata (63,33%) maupun R. mucronata (83,33%). Pemangsaan propagul R. mucronata paling tinggi di stasiun yang didominasi R. mucronata (95%) dibandingkan di stasiun yang di dominasi R. apiculata (55%) maupun R. stylosa (45%). Pemangsaan propagul R. apiculata paling rendah di stasiun yang didominasi R. apiculata (50% ) dibandingkan di stasiun yang di dominasi R. stylosa (70%) maupun R. mucronata (73,33%). Hasil penelitian ini berhasil membuktikan teori dominance-predation pada spesies R. stylosa dan R. apiculata, namun tidak pada R. Mucronata. Kata kunci: pemangsaan, propagul, pra-penyebaran, paska-penyebaran, mangrove Propagule predation on mangrove in some extent reduced its viability to grow into seedling. The predation could happened before (pre-dispersal) or after (post-dispersal) the propagule drop from the tree.The reasearch was conducted in Pasar Banggi, Rembang District, Central Java. The aim was to investigate the predation rate of Rhizophora mucronata Lamk., R. stylosa Griff. and R. apiculata Blume propagules pre-dispersal and post-dispersal. Firstly, preface experiment for find domination spesies in the location, Second, with applied descriptive-based survey sampling and field experiment methods. Than all propagules of five replication trees were harvested and checked for its condition on pre-dispersal step. The third, with post-dispersal study there were twenty propagules from each spesies and tied them with used nylon string and placed on the forest floor for 2 until 18 days and checked its condition every 2 days after placement. This study is also set for tested the Smith’s theory on propagule predation related to tree domination. Rhizophora stylosa propagule was most predated before they fall (mean 61,06%, range 45,40-76,05%), followed by R. apiculata (mean 58,18%, range 47,41-68%) and the lowest isR. mucronata with mean 11,88% (range 7,06-15,71%). After 18 days of experiment in the field R. stylosa propagule in R. stylosa–dominated area was the lowest predated (mean 46,67%) compared to propagule in the area dominated by R. apiculata (63,33%) and also in R. mucronata area (83,33) Predated R. mucronata propagule is the highest in the R. mucronata dominated area (mean 95%) compared with R. apiculata dominated area (mean 55%) and also in R. stylosa dominated area (45%). Pradated of R. apiculata propagule is the lowest in the domination area of R. apiculata (50% ) compared with R. stylosa area domination with (mean 70%) also R. mucronata (73,33%). The result showed that the theory of dominance-predation can be proved only for R. stylosa and R. apiculata spesies, but not for R. mucronata spesies. Keywords : propagule, predation, pre-dispersal, post-dispersal, mangrove

Phytoplankton community structure and it’s relationships with water quality in Bangka Island, Indonesia Muh Yusuf; Robin Robin; Wahyu Adi; Mu’alimah Hudatwi; Widianingsih Widianingsih; Retno Hartati; Robertus Triaji Mahendrajaya; Cristiana Manullang
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 1 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.1.37-44

Phytoplankton plays an important role in primary productivity in marine environment. Various environmental changes in coastal area will impact the water quality and their phytoplankton compositions. The purpose of this study is to examine the abundance of phytoplankton from two different sites, i.e Tanah Merah (close to mining site) and Semujur Island (away from mining site) in Bangka Island. Phytoplankton and water sample were collected on June- August 2018. Water quality was measured using water quality checker, whereas the phytoplankton was identified under the microscope with a magnification of 100x. Non-parametric Kruskal test and T-test analysis was performed to determine the abundance, diversity, uniform, and dominance of phytoplankton between Sites, respectively. Statistical analyses showed the abundance of phytoplankton at Semujur Island was significantly higher than that at Tanah Merah (p = 0.003). In additions the diversity, uniform, and dominance were also significantly different between sites (all p <0.05). In Semujur Island, Diatoms (Thalassiothrix, Chaetoceros and Thalassionema) were more dominants than the Dinophyceae group. However, in Tanah Merah, the genera Ceratium belong to class Dinophyceae was more dominant than the class Bacillariophyceae. These results performed that the phytoplankton in Tanah Merah and Semujur Island was affected by environment, in this case the mining area. The water quality in Semujur Island (non-mining Area) might have good quality than in Tanah Merah (mining area). The average value of turbidity and Total Suspended Solid in Tanah Merah Waters causes low abundance of phytoplankton. It can be concluded that tin mining can disrupt the abundance and composition of phytoplankton as a primary producer of waters.

Distribusi dan Kelimpahan Polychaeta di Kawasan Hutan Mangrove Klaces dan Sapuregel, Segara Anakan, Cilacap Agus Indarjo; Widianingsih Widianingsih; Ari B Abdulah
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 1 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Tingginya produktivitas mangrove menjadikan hutan mangrove memiliki fungsi ekologis yang pentingbagi makhluk hidup, yaitu sebagai tempat memijah, pembesaran dan mencari makan. Biota yang hiduppada ekosistem mangrove antara lain ikan, udang, kepiting, moluska dan polychaeta. Tujuan penelitian ini adalah untuk mengetahui distribusi dan kelimpahan polychaeta di daerah Klaces dan Sapuregel yang memiliki perbedaan tingkat sedimentasi. Hasil penelitian telah ditemukan 20 famili polychaeta, 5 famili di Klaces dengan kelimpahan 44,68 ind/ m2 dan 17 famili di Sapuregel dengan kelimpahan 88,38 ind/m2. Biomassa untuk 4 famili yang dominan di kedua lokasi penelitian tidak memiliki perbedaan yang signifikan kecuali pada famili Capitellidae yaitu 0,026 g/ind di Klaces dan di Sapuregel 0,012 g/ind. Indeks keanekaragaman (H’) di Klaces dan Sapuregel masing-masing adalah 0,49 (keanekaragaman famili rendah) dan 1,11 (keanekaragaman famili sedang), serta indeks keragamannya (e) 0,27 dan 0,30 (keseragamanfamili rendah). Indeks dominasi (C) di Klaces 0,64 (terdapat famili yang mendominasi) lebih besar dariSapuregel yaitu 0,33(tidak terdapat famili yang mendominasi).Kata kunci : polychaeta, distribusi, kelimpahan, mangroveThe high productivity in mangrove area has ecological function which is important for other living organisms, among others is for spawning area, nursery and feeding area. The biota which is live in mangrove ecological system are fish, shrimp, crab, mollusc, and polychaeta. The objective of this research is to look for the distribution and abundance of polychaeta in Klaces and Sapuregel area where are have difference sedimentation level. Twenty polychaeta families were found in this research, 5 families in Klaces and 17 families in Sapuregel. Abundance of polychaeta in Klaces was 44,68 ind/m2, this was fewer than in Sapuregel. The biomass for 4 families which was dominant in two research area were not have significant different, except Capitellidae. Biomass Capitellidae was 0,026 g/ind in Klaces and 0,012 g/ind in Sapuregel. Diversity Index (H’) in each research area Klaces and Sapuregel were 0,49 (low diversitylevel) and 1,11 (moderate diversity level) with evenness index (e) 0,27 and 0,3 (low evenness indexlevel). Domination index (C) in Klaces was 0,64 and in Sapuregel was 0,23. This value shows thatdomination index Klaces was more than Sapuregel.Key words : polychaeta, distribution, abundance, mangrove

Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus Delianis Pringgenies; Endah Sari; Widianingsih Widianingsih; Anuwat Nateewathana
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 3 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.3.182-188

Namanereis sp. belongs to class Polychaeta, a group of main benthic community in marine ecosystems. The purpose of this study to determine the potential of bioactive compounds from Namanereis sp. extract as an antibacterial agent and to determine its extract content through Gas Chromatography–Mass Spectrometry (GC-MS) analysis. Samples of Namaneries sp. were collected from Demak Waters, Central of Java. This research used experimental laboratory method. Samples were extracted with non-polar (N-Hexane), semi-polar (Ethyl acetate) and polar (Methanol) solutions. Different concentrations of extraction, i.e. 5, 10, 20, 40, 80, and 100 µg.disk-1. then were tested against pathogenic bacteria Escherichia coli and Staphylococcus aureus. The extract content analysis was obtained using the GC-MS method. The results showed that Namanereis sp. extract had a potential as an antibacterial agent. The highest inhibition zone was seen in the fraction with ethyl acetate solvent at a concentration of 100%, namely 13±3.05 mm in E. coli and 14±3.04 mm in S. aureus, which then was analysed with GC-MS. Its results found 20 bioactive compounds in the extract. The five most dominant compounds determined by the highest peak were Dihydrojasmone (12.31%), Hexanoic acid (11.49%), Cholesterol (7.66%), Cyclohexanol (7.53%) and Isopropyl Palmitate (7.52%). The research concluded that Namanereis sp. possessed antibacterial potency and contained several bioactive compounds.

Kandungan Lipid Total Nannochloropsis oculata Pada Kultur dengan Berbagai Fotoperiod (Total Lipid Content of Culture of Nannochloropsis oculata at Different Photoperiod) Widianingsih Widianingsih; Retno Hartati; Endrawati Hadi; Valentina R Iriani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 3 (2012): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Fotoperiod memainkan peranan penting dalam proses fotosintesis pada mikroalga Nannochloropsis oculata. Fotoperiod akan mempengaruhi kadar total lipid pada berbagai jenis mikroalga. Penelitian ini bertujuan untuk determinasi kandungan lipid total mikroalga Nannochloropsis oculata yang dikultur pada berbagai kondisi fotoperiod Rancangan acak lengkap dengan tiga ulangan diterapkan pada penelitian ini. Perlakuan pada penelitian ini adalah fotoperiod 4 jam terang-20 jam gelap, 8 jam terang-16 jam gelap, 12 jam terang-12 jam gelap, dan 24 jam terang. N. oculata dikultur pada erlenmeyer 250 mL dengan sistem aerasi kontinyu dan pencahayaan 3000 lux, salinitas 33 ppt dan medium Conway. Hasil penelitian menunjukkan bahwa kandungan lipid total pada N. oculata tertinggi ditemukan pada perlakuan fotoperiod 12 jam terang-12 jam gelap) (31,8±3,03%-dw) dan kemudian diikuti pada fotoperiod 8jam terang - 16 jam gelap (25,2±2,19%-dw) dan fotoperiod 24 jam terang (23,2 %-dw). Pada perlakuan fotoperiod 8 jam terang-16 jam gelap dan fotoperiod 12 jam terang-12 jam gelap pada media pemeliharaan N.oculata menunjukkan hasil kadar lipid total yang lebih besar pada fase stasioner dibandingkan dengan eksponensial.Kata kunci: Nannochloropsis oculata, total lipid, fotoperiodPhotoperiod has important role on the photosynthesis process of microalgae Nannochloropsis oculata. Photoperiod also influences on total lipid content of various microalgae. This research has purpose to examine the effect of nutrient phosphate and nitrate composition to the total lipid content of N. oculata belong to class Eustigmatophyceae. There were four treatments of photoperiod as follows; (a) photoperiod A (4:20 hour light:dark);(b) photoperiod B (8:16 hour light:dark); photoperiod C (12:12 hour light:dark); (and (d) photoperiod D (24 hour light). There were three replicates for each treatment. The volume of culture medium was 250 mL for each treatment with continuously aeration and illumination (3000 lux). According to the research, the highest total lipid content of N. oculata had been found on the treatment of photoperiod 12:12 hour light:dark 31,8 ± 3,03 %-dw, and then followed by photoperiod 8:16 hour light dark as amount 25,2 ± 2,19 %-dw and 24 hour light (23,2 ± 1,99%-dw), and then followed by photoperiod 24 hour light. Differences of photoperiod 8:16 hour light:dark and photoperiod 12:12 hour light:dark on culture medium of N. oculata showed result that there were differences of total lipid content on the stationary and exponential phase. The highest percentage of total lipid was fund in cell of N. oculata grown under treatment of photoperiod 12:12 hour light:dark. The treatment of photoperiod 8:16 hour light:dark and 12:12 hour light:dark in the N. oculata culture showed result that the total lipid content on stationary phase was greater than exponential phase.Key words: Nannochloropsis oculata, total lipid, photoperiod

Relationship Between Stage of Gonad Maturity and Level of Osmotic Work of Sea Cucumber, Paracaudina australis Widianingsih Widianingsih; Muhammad Zainuri; Sutrisno Anggoro; Hermin Pancasakti Kusumaningrum; Retno Hartati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 1 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The holothuroid spawning can be succesful depend on varoius factors such as salinity, temperature, primary productivity and other physiological adaptation toward environment influences. The salinity fluctuation is one of the environment factors, which influence and can cause various osmotic pressure of coelomic fluid of Holothoroid and also gonad maturity stage. Holothuroid (sea cucumber), Paracaudina australis, is overexploited in Kenjeran waters, Surabaya, East Java. The study was aimed to reveal relationship between gonad maturity stage and level of osmotic work of sea cucumber P. australis. Fifty samples of P. australis were collected monthly. Coelomic fluid samples of approximately 200–1000 µl were obtained from all samples using disposable insulin syringes. There were 37 holothuroids classified in the late maturity (stage 5), 74 holothuroid in the earlier maturity stage (stage 1). Gonad maturity stage 5 (late maturity) could be reached on December 2016. Contrasly, the gonad maturity stage 1 (earlier maturity) was found on August 2016. The analysis of coelomic fluid showed that the average value of level of osmotic work ranged 70 to 91 mOsm/L H2O in the earlier gonad maturity stage. Contrasly, in the late maturity, the average value of level of osmotic work ranged 118 to 156 mOsm/L H2O. There is relationship between gonad maturation stage and level of osmotic work. From stage of gonad maturity 1 to 5, there were upward trend level of osmotic work in coelomic fluid of P. australis.

Fission Reproduction of Two Stichopudidae Species (Holothuria:Echinodermata) (Reproduksi Fission dari Dua Species Teripang Famili Stichopudidae (Holothuria:Echinodermata) Retno Hartati; Widianingsih Widianingsih; Pradina Purwati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 2 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Terdapat dua sistem reproduksi pada teripang, yaitu seksual dan aseksual. Fission, sebagai salah satu cara reproduksi aseksual, adalah kemampuan alami teripang untuk membelah tubuhnya menjadi dua bagain dan masing-masing bagian dapat beregenerasi menjadi individu baru. Fenomena ini memberikan kemungkinan melakukan simulasi fission untuk memperbanyak benih teripang. Penelitian ini bertujuan untuk menganalisa kemampuan fission dua species Stichopudidae (Stichopus hermanii and S. chloronotus) dari Karimunjawa. Simulasi fission dilakukan dengan mengikat teripang uji dengan karet pentil dan meletakkan individu teripang yang telah diikat pada keranjang yang digantung pada permukaan laut. Pengamatan fission dilakukan pada jam ke 12, 24 dan 48. Ketika telah terjadi fission dan luka tertutup, teripang uji tetap berada di keranjang uji dan teripang tidak diberi pakan. Perlakuan berlanjut sampai penyembuhan secara morfologis terjadi dengan sempurna. Waktu terjadinya regenerasi setelah luka sembuh dicatat sebagai data pengamatan. Hasil penelitian menunjukkan bahwa simulasi fisssion dapat dilakukan pada species teripang Stichopudidae. Setelah proses fission selesai, dinding tubuh pada lokasi fission menjadi basah atau berlendir selama satu sampai dua jam. Pada pagi hari berikutnya, dinding tubuh akan nampak normal konsistensinya dan luka telah tertutup. Proses fission berlangsung selama beberapa jam sampai dua haridan tanda-tanda regenerasi timbul mulai dua sampai tiga minggu setelah fission. Waktu generasi S. hermanii terjadi lebih cepat (2 minggu setelah fission) daripada S. chloronotus. Kata kunci : fission, produksi benih, stichopudidae, Pulau Karimunjawa There are two reproduction systems in seacucumber, i.e. sexual and asexual. Fission, as a way of asexual reproduction, is natural seacucumber ability to split their body into two part, and this natural fenomenon give possibility to conduct fission stimulation as seacucumber propagation. Present works are aimed to analyse fission capability of two Stichopudidae species of sea cucumber (Stichopus hermanii and S. chloronotus) from Karimunjawa Islands. Fission stimulation by rubber band tied was done in basket hanged on the sea cage. The fission was observed for 12, 24 and 48 hours. When fission occurred and wound recovered the sea cucumber were still kept in the basket and no food was added. This treatment continued until morphological recovery was completed. The time regeneration occurred after wounds recover was recorded. The result of present work revealed that stichopudidae species showed very good response to fission stimulation. After the entire process of fission, the bodywall at the fission site remained a liquid or mucus like consistency for at least two more hours. The following morning, the bodywall had its normal consistency and the wounds at both ends were nearly entirely closed. The fission process took several hours to two days and signs of regeneration appeared two to five weeks after fission. Fission simulation give shorter regeneration time for S. hermanii (2 week) than S. chloronatus. Keywords: fission, seed production, stichopudidae, Karimunjawa island

Penempelan Larva Teripang Putih (Holothuria scabra) pada Substrat yang Berbeda Retno Hartati; Widianingsih Widianingsih; Delianis Pringgenies; Nur Taufiq; Ali Djunaedi; Sari Budi Moria
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 7, No 3 (2002): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Metamorfosis pada larva teripang diikuti dengan perubahan tingkah lakunya. Stadia auricularia bersifat planktonic sedangkan stadia akhir doliolaria dan awal pentactula bersifat bentik dan memerlukan substrat untuk menempel. Perubahan ini merupakan saat kritis dalam pembenihan teripang sehingga substrat yang sesuai perlu diberikan pada media pemeliharaan larva. Penelitian ini bertujuan untuk mengetahui kesuakaan penempelan larva teripang putih (H. scabra) pada substrat asbes, kasa plastik dan kaca. Penghitungan densitas larva yang menempel pada tiap substrat dilakukan sejak larva mulai menempel pada hari ke-25 hingga larva mencapai stadia juvenil-umur 35 hari. Hasil penelitian menunjukkan bahwa substrat memberikan pengaruh yang berbeda nyata (P>0.05) terhadap jumlah larva yang menempel. Larva lebih banyak menempel pada substrat asbes (rata-rata 425,28 individu.m2 dengan kelulushidupan 9,96 % tetapi substrat kaca paling sedikit ditempeli larva (85,18 individu.m2 dengan kelulushidupan 3,98%). Perbedaan jumlah larva yang menempel pada perlakuan substrat diduga berkaitan dengan kekasaran permukaan substrat.Kata kunci: larva teripang putih; penempelen; substrat The metamorphoses of seacucumber larvae indicate changing in their behaviour. Auricularia stage is planktonic but late doliolaria and early pentactula are benthic and need suitable substrate. It is a critical period in development and culture of seacucumber larvae since the biggest mortality happened during metamorphosis and settlement therefore the suitable substrata need to be provided. The aims of the experiment was to determine larvae's preference on substrate made of asbestos, plastic screen and glass. Density of larvae settled on the substare were counted on day 25 up to juvenile stage of 35 days old. The results showed that the treatment gave significant differences on larvae settlement on the substrate. The larvae found attached on asbestos was highest (425,28 ind.m2 and survival rate 9.96 %) and lowest number larvae settled on glass (85,18 ind.m2 and survival rate 3,98 %). It seemed due to difference roughness of surface.Keywords: larvae seacucurnber; settlement; substrats

Hubungan Panjang Berat Teripang Holothuria atra di Pulau Panjang, Jepara Primaswatantri Permata; Suryono Suryono; Frijona F. Lokollo; Widianingsih Widianingsih; Hadi Endrawati; Muhammad Zainuri; Retno Hartati
Buletin Oseanografi Marina Vol 10, No 2 (2021): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v10i2.36380

Holothuria atra merupakan salah satu teripang dan sering dijumpai berasosiasi dengan padang lamun dan substrat pasir. Walaupun bernilai ekonomi rendah, secara ekologi mempunyai manfaat sebagai bioturbator dan mempunyai potensi dalam bidang farmaseutical. Teripang ini ditenukan hidup di Pulau Panjang, dengan karakteristik perairan tertutup dan dangkal dengan akses yang mudah untuk wisatawan maupun perikanan tangkap yang dapat menjadi tekanan bagi populasi teripang tersebut. Penelitian ini bertujuan untuk untuk mengetahui hubungan panjang dan berat populasi teripang H. atra di Pulau Panjang, Jepara pada lokasi padang lamun dan pecahan karang dimana H. atra banyak ditemukan. Metode penelitian yang dipergunakan adalah metode deskriptif eksploratif dengan melakukan pengambilan sampel H. atra dengan teknik sampling complete enumeration pada luas lokasi penelitian yang telah ditentukan. Hasil penelitian menunjukkan kepadatan teripang di stasiun padang lamun (Stasiun A) lebih rendah dengan distribusi ukuran yang lebih kecil daripada di stasiun pecahan karang (Stasiun B). Pada semua stasiun dan waktu pengambilan sampel, pola pertumbuhan relatif yang ditunjukkan oleh hubungan panjang dan berat teripang H. atra bersifat allometrik negatif dengan keeratan hubungan yag sangat kecil. Hal ini disebabkan oleh kesulitan pengukuran pada teripang yang bertubuh lunak dengan elastisitas dinding tubuh (integument), isi pada sistem pencernaan makanannya dan kandungan cairan coelomic (rongga tubuh) yang dapat membuat bias analisa.Holothuria atra is one of species sea cucumbers often found in association with seagrass beds and sand substrates. Although it has low economic value, ecologically it has benefits as a bioturbator and has potential in the pharmaceutical. It is found in Panjang Island, which has characteristic of closed and shallow waters with easy access for tourists and capture fisheries which can put pressure on the sea cucumber population. This study aimed to determine the relationship between length and weight of H. atra population in the seagrass bed and rubble area in Panjang Island, Jepara. The research method used is descriptive exploratory and H. atra were sampled using a complete enumeration sampling technique at a predetermined area of the research location. The results showed that the density of sea cucumbers at the seagrass beds station (Station A) was lower with a smaller size distribution than at the rubble station (Station B). At all stations and sampling times, the relative growth pattern shown by the correlation between length and weight of H. atra is negative allometric with very small relationship value. This was due to the difficulty of measuring soft-bodied sea cucumbers with the elasticity of the body wall (integument), the contents of the digestive system and coelomic fluid in the body cavity which can bias the analysis.

Densitas dan Kadar Total Lipid Mikroalga Spirulina platensis yang Dikultur pada Fotoperioda yang Berbeda Hadi Endrawati; Christin Manulang; Widianingsih Widianingsih
Buletin Oseanografi Marina Vol 1, No 3 (2012): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Spirulina platensis tergolong kedalam Divisi Cyanophyta dengan kemampuan adaptasi yang baik terhadap pengaruh faktor lingkungan yang cukup bervariasi seperti suhu, salinitas, intensitas cahaya, nutrien dan fotoperioda. Faktor lingkungan dapat mempengaruhi komposisi dan kadar lipid, protein, dan karbohidrat. Lipid berfungsi sebagai penyedia asam lemak dan sumber energi cadangan. Penelitian ini bertujuan untuk optimasi kadar total lipid dan densitas dari mikroalga Spirulina platensis yang dikultur pada fotoperioda berbeda. Penelitian ini menggunakan Rancangan Acak Lengkap dengan perlakuan fotoperioda 4 jam terang 20 jam gelap, 8 jam terang 16 jam gelap, 12 jam terang 12 jam gelap, dan 24 jam terang. Hasil penelitian menunjukkan bahwa kepadatan S. platensis tertinggi pada perlakuan 24 jam terang, yaitu (1591 ± 16) x 103 sinusoidal/l pada fase stasioner dan kepadatan terendah pada perlakuan 4 jam terang 20 jam gelap yaitu (1087 ± 62) x 103 sinusoidal/l. Selanjutnya total lipid tertinggi pada fotoperioda 4 jam terang 20 jam gelap (46,1 ±27,93 %-dw) dan total lipid terendah pada perlakuan 24 jam terang sebesar (24,8 ±5,23 %-dw). Kata kunci: Spirulina platensis, densitas, fotoperiod, total lipid

Co-Authors Abdul Hadi Abdullah Afif Abidin Nur II Achmad Muhajir Adi Santoso Agus Indarjo Agus Subagio Agus Trianto Aini, Salsabila Quratu Aldhian Triatmojo Aldi Rivaldy Maulana Ali Djunaedi Ali Djunaedi Altysia Putriany Ambariyanto Ambariyanto Anak Agung Istri Sri Wiadnyani Annisa Fadillah Antonius Budi Susanto Anuwat Nateewathana Apriliani, Seka Indah Ari B Abdulah Aris Ismanto Arumning T. Fauziah Azhari Nourma Dewi Bagus Enggal Prakoso Broto Wisnu RTD Broto, R. T.D Wisnu Cantika Elistyowati Andanar Chamidy, Ardian Nurrasyid Chrisna Adhi Suryono Christin Manulang Cristiana Manullang Cristiana Manullang Cristiana Manullang Dedi Nugroho Deki Lukman Wicaksono Delianis Pringgenies Denny Nugroho Sugianto Donna Nur'Aurelya Mahardhika Dwi Haryanti Eddy Yusuf, Eddy Edy Supriyo Edy Supriyo Edy Supriyo ELza Lusia Agus Emia Sayniri Sembiring Endah Sari Endang Supriyantini Ervia Yudiati Farmasita B, Rizky Farmasita Budiastuti, Rizky Fathorrahman Fauzan, Rianda Febriana Banun Fitrianti Fitri Kurniawati Fitri Kurniawati Fitriyan, Jodhi Kusumayudha Frijona Fabiola Lokollo Ginzel, Fanny Iriany H. Endrawati Hadi Endrawati Hayati, Amaliya Tsiqotul Hendrik Surya Bahar Hermawan Hermawan Hermawan Hermawan Hermin Pancasakti Kusumaningrum Hilal M Ibnu Pratikto Imam Misbach Imam Mishbach Indras Marhaendrajaya Irma Kusumadewi Irwani Irwani Ita Riniatsih Ita Widowati Jelita Rahma Hidayati Johannes Hutabarat Jusup Suprijanto Ken Suwartimah Kurnia, Andine Rizki La Nina Gunaswara Samudera Mada Triandala Sibero Mahfud Mahfud Meitri Bella Puspa Melinda Sri Asih Mirsa Septiana Mutik Mostafa Imhmed Ighwerb Muchammad Miftahul Ulum Muchammad Miftahul Ulum Muhammad Helmi Muhammad Iskandar Zulkarnain Muhammad Yusuf Muhammad Zainuri Muhammad Zainuri Muhammad Zainuri Muhammad Zainuri Muhammad Zainuri Munana, Nila Munasik Munasik Munnaffaroh, Mufasihatul Mu’alimah Hudatwi Nando Arta Gusti Pamungkas Nila Munana Njurumana, Steven Nggiku Nopratilova Nopratilova Nur Taufiq Nur Taufiq-Spj Nurhabibah, Prabawati Ony Ilham Pradiksa Pradina Purwati Prakoso, Bagus Enggal Pratiwi, Wukir Berliana Primaswatantri Permata Puji Norbawa Putri Sakinah Mayani, Putri Sakinah Putri, Ni Putu Purba Nava Vidyadhari Raden Ario Rafsanjani A. Karim Rafsanjani A. Karim Ranny Ramadhani Yuneni Retno Hartati Retno Kusumastuti Retno Murwani Ria Azizah Ria Azizah Tri Nuraini Rico Adi Setyanto Robertus Triaji Mahendrajaya Robertus Triaji Mahendrajaya Robertus Triaji Mahendrajaya Robertus Triaji Mahendrajaya Robin Robin Rudhi Pribadi Sabila, Ahda Samudera, La Nina Gunaswara Saputra Giri Wicaksono Saputra Giri Wicaksono Saputri, Noviyani Sari Budi Moria Septiyani, Fenny Seto Haryoardyantoro Siagian, Cristiani Sri Redjeki Sri Turni Hartati Sri Turni Hartati Sri Yulina Wulandari Subagiyo Subagiyo Sugiyanto, Nenden Rose Sunaryo Sunaryo Sunaryo Sunaryo Surbakti, Fitriani Suryono Suryono Sutrisno Anggoro Theresia Claudia Lasmarito Theresia Pradiani Triatmojo, Aldhian Ulfah Nurjanah, Ulfah Valentina R Iriani Valentina R. Iriani W.L. Saputra Wahyu Adi Wahyu Dewi Utari Haryanti Wilis Ari Setyati Wisnu Dewanto Wita Kristianty Sirait Wukir Berliana Pratiwi Yulia Ulfah Yulia Ulfah Yuvita Muliastuti

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