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All Journal HAYATI Journal of Biosciences Jurnal Sain Veteriner Buletin Peternakan Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease TIJAB (The International Journal of Applied Business) Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Veterinary Biomedical and Clinical Journal Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Jurnal Abdidas journal of Basic Medical Veterinary Current Biomedicine Jurnal Agro Veteriner
Adiana Mutamsari Witaningrum
Department Of Veterinary Public Health, Faculty Of Veterinary Medicine, Airlangga University, Surabaya, 60115, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Identification and Prevalence of Endoparasite on Layer Chicken in Udanawu Sub-district Blitar Khalissa Farah Alifia; Setiawan Koesdarto; Yulianna Puspitasari; Mufasirin; Adiana Mutamsari Witaningrum
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.38769

Abstract

The aim of this research was to determined the prevalence and infection degrees of endoparasite on layer chicken in Sub-district Udanawu, Blitar. Ninety-six samples were taken from layer chicken in 3 different villages namely Bakung Village, Tunjung Village, and Slemanan Village. The examinations taken in this study are fecal examination using native, sediment, and floating methods and blood examination using blood smear method. Result showed that 81.25% samples are positive for helminthiasis infection consisting of Ascaridia galli (66.67%), Heterakis gallinarum (45.83%), Raillietina sp. (31.25%), and Strongyloides avium (7.29%). Blood examination result shown there is no positive sample that infect layer chicken in Sub-district Udanawu, Blitar. Chi-Square test result showed there are significant difference (P<0.05) of Ascaridia galli and Heterakis gallinarum in Bakung Village, Tunjung Village, and Slemanan Village in Sub-district Udanawu, meanwhile there are no significant difference (P>0.05) of Raillietina sp. and Strongyloides avium. Range of infection degrees of helminthiasis in Bakung village, Tunjung village, and Slemanan Village are 608.75 ± 588.53, 223.12 ± 359.21, 156.25 ± 332.39. There are significant difference (P<0.05) on helminthiasis infection degree of layer chicken in Udanawu District, Blitar.
Total plate count dalam isi telur ayam ras di pasar tradisional Kecamatan Waru, Kabupaten Sidoarjo Budiarto, Budiarto; Kirana, Sabila Citra; Lestari, Tita Damayanti; Budiarto; Puspitasari, Yulianna; Mutamsari Witaningrum, Adiana; Permatasari, Dian Ayu
Current Biomedicine Vol. 1 No. 2 (2023): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.2.86-94

Abstract

Background: Microbial contamination in consumed chicken eggs can endanger human health. Bacteria can grow and develop in food so that it can reduce the food quality. Determination of food quality can be done by various methods, one of which is Total Plate Count (TPC). Objective: The aim of this study was to determine the level of bacterial contamination in chicken eggs sold at traditional markets in Waru District, Sidoarjo Regency. Methods: Thirty samples were collected from two traditional markets, Waru Market and Wadung Asri Market. This study used the pour plate method of TPC test. Egg contents were diluted and then inoculated in Nutrient Agar. The media inoculated with the sample suspension was incubated at 37°C for 24 hours. The growing bacterial colonies were counted and analyzed using the Standard Plate Count. This study's data are presented descriptively and compared with the Indonesian National Standard (SNI) 7388-2009 maximum requirement of contaminant bacteria in chicken eggs is 1×105 CFU/mL. Results: The results showed that the averages TPC in two traditional markets were 2.1×104 CFU/mL in Waru Market and 3.4×103 CFU/mL in Wadung Asri Market. The average TPC still meets the maximum limit of microbial contamination listed in SNI 7388-2009. Conclusion: The average values of TPC in both markets are not the same, but the qualities of chicken eggs sold in Waru Market and Wadung Asri Market are good and suitable for consumption.
Identification of Digestive Tract Endoparasites of Laying Hens in Suruhwadang Village, Kademangan District, Blitar Regency Mega, Toafan Satrio; Susilowati, Suherni; Hastutiek, Poedji; Kusnoto, Kusnoto; Sunarso, Agus; Witaningrum, Adiana Mutamsari
Journal of Parasite Science Vol. 8 No. 1 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i1.52373

Abstract

This study aims to determine the species, prevalence, and infection degree of endoparasite in the digestive tract of laying hens in Suruhwadang Village, Kademangan District, Blitar Regency. Ninety-six fecal samples were taken from four different farms. Twelve samples of chicken feces aged 20-50 weeks and twelve samples of chicken feces > 50 weeks were taken from each farm. The examinations taken in this study are fecal examinations using native, sediment, and floating methods. The results of identification of digestive tract endoparasites found consisting of Ascaridia galli (68.75%), Heterakis gallinarum (53.12%), Strongyloides avium (5.21%), Trichostrongylus tenuis (6.25%), Eimeria maxima (51,04%), and Eimeria acervulina (3,12%). The highest average degree of worm egg infection was found in layers >50 weeks of age of 373.96 ± 450.41 while the highest average degree of infection of protozoa was found in layers aged >50 weeks of 296,87 ± 600,92. The results of the Chi-Square test showed that there was no significant effect of the difference in the age of laying hens in the layer phase on the prevalence of digestive tract endoparasites, both worm egg and protozoan infections (P>0.05). The results of the Mann-Whitney test showed that there was a significant effect of the difference in the age of the laying hen in the layer phase on the degree of worm egg infection (P<0.05), but the difference in age of the laying hen in the layer phase did not significantly affect the degree of protozoa infection (P>0,05).
Identification, Prevalence, and Infestation Patterns of Ectoparasitic In MP Hybrid Type Culled Laying Ducks In Gedang Sub Village, Modopuro Village, Mojosari Sub District, Mojokerto District Aurandini, Nailla Ridhani; Hastutiek , Poedji; Utama , Suzanita; Sunarso, Agus; Suprihati , Endang; Witaningrum, Adiana Mutamsari
Journal of Parasite Science Vol. 8 No. 1 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i1.53342

Abstract

The purpose of this study was to identify ectoparasites and to determine the prevalence and infestation patterns of ectoparasites that attack the MP hybrid type culled ducks in Gedang Hamlet, Modopuro Village, Mojosari District, Mojokerto Regency. This study used a sample of 100 culled laying ducks of the MP hybrid type. The obtained ectoparasites were identified at the labaroratory of division parasitology, Faculty of veterinary medicine, Universitas Airlangga, Surabaya, using the permanent mounting method without staining and viewed using a microscope with 40-100x magnification. This study found 71 samples positively infested with ectoparasites out of 100 samples of culled MP hybrid ducks examined with (prevalence of 71%). The types of ectoparasites found in this study were Menacanthus stramineus (25%), Menopon gallinae (9%), and Anaticola crassicornis (59%). Correspondence analysis test was conducted to show the pattern of infestation of the ectoparasites. The head-neck region was infested by M. stramineus and M. gallinae, while the back, wings, abdomen and tail regions were infested by A. crassicornis. A. crassicornis mostly attacked the MP hybrid type of culled laying ducks on the wings, compared to the back, abdomen and tail. A. crassicornis is an ectoparasite that is typical for the wing region, while M. gallinae and M. stramineus are ectoparasites that are typical for the head-neck region.
THE PREVALENCE OF HUMAN IMMUNODEFIENCY VIRUS-1 (HIV-1) SUBTYPES AND TRANSMISSION METHOD AMONG HIV/AIDS INFECTION PATIENT IN TULUNGAGUNG, EAST JAVA INDONESIA Ardianto, Achmad; Khairunisa, Siti Qamariyah; Kotaki, Tomohiro; Witaningrum, Adiana Mutamsari; Qushay, M.; Juniastuti, Juniastuti; Rahayu, Retno Pudji; Widiyanti, Prihartini; Utomo, Budi; Lusida, Maria Inge; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 5 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.969 KB) | DOI: 10.20473/ijtid.v5i5.305

Abstract

The rapid epidemic growth of HIV is continuing in Indonesia. There are some factors which have influenced the spreading of this epidemic in Indonesia, such as the poor awareness to avoid unsafe free sex attitude and the sharing of needles and syringes among intravenous drug users (IDUs). The sexual transmission of HIV has also apparently increased in Tulungagung. Commercial sex workers play a significant role in the spread of HIV in Tulungagung. People in Tulungagung have worked at other countries as Indonesian migrants. This condition can cause the increase number of HIV-1 case and the possibility of genetic variation (subtype) HIV-1 in Tulungagung. This research is aimed to analyze the subtype and to determine estimation of transmission mode on infected patient of HIV-1 and AIDS who came to Seruni clinic Dr. Iskak hospital in Tulungagung. 40 HIV?AIDSpatients were interviewed to determine the subtype and the transmission mode. The results showed that 14 of 40 plasma samples (35%) were successfully to amplified and sequenced. OverallCRF01-AE wereidentified as predominant subtype among HIV/AIDS patients in Tulungagung. Based on individual information, 31 of 40 subjects (77%) were heterosexual transmission.
Identification and The Prevalence of Gastrointestinal Endoparasite in Long-Tailed Macaque (Macaca Fascicularis) in Wonorejo and Gunung Anyar Mangrove Eco-Tourism Surabaya Fauziyah , Nafisah Nurti; Lastuti , Nunuk Dyah Retno; Damayanti , Ratna; Koesdarto , Setiawan; Suprihati , Endang; Witaningrum, Adiana Mutamsari
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.5

Abstract

This study aimed to determine the types of endoparasites and their prevalence in Macaca fascicularis in the Wonorejo and Gunung Anyar mangrove forests, Surabaya. A total of 90 samples of Macaca fascicularis faeces were collected from two different mangrove forests, 45 samples from Wonorejo and 45 from Gunung Anyar. All samples were examined using sedimentation and floating methods to identify endoparasite species. Further, the number of eggs and oocysts per gram of feces was counted using the McMaster method. Results showed that 26.67% (12/45), and 22.2% (10/45) of the faecal samples were detected positive for endoparasite at Wonorejo and Gunung Anyar mangrove forests, respectively. The prevalence of parasitosis in M. fascicularis from Wonorejo was 17.7% (8/45) for Taenia sp. and 8.8% (4/45) for Balantidium coli cyst. On the other hand, M. fascicularis from Gunung Anyar was infested by Trichuris sp. at a prevalence of 22.2% (10/45). The total number of eggs and cysts ranged from 30 – 400 per gram of faecal samples. The study provides new knowledge on the parasitic burdens among long-tailed macaques in East Java.
Effect of Plectranthus scutellarioides (L.) Leaf Extract as Natural Antibacterial Against Staphylococcus aureus and Escherichia coli Isolated From Dairy Cattle with Subclinical Mastitis Shafina Hanum, Farah; Witaningrum, Adiana Mutamsari; Puspitasari, Yulianna
Journal of Basic Medical Veterinary Vol. 11 No. 2 (2022): Journal of Basic Medical Veterinary, December 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i2.39799

Abstract

Mastitis is one of the causes of low milk production in dairy cows, and can be caused by bacteria such as Escherichia coli and Staphylococcus aureus. Plectranthus scutellarioides (L.) leaf has antibacterial compounds such as flavonoids, eugenol, polyphenols, steroids, tannins, and so on. The type of this research is experimental laboratory research that aims to test the antibacterial activity of the P. scutellarioides (L.) leaf extract with ethanol pro analysis as the solvent against E. coli and S. aureus bacteria isolated directly from the sample of dairy cow's milk which CMT score is (+) 3, by measuring its inhibition ability indicated by the presence of clear zone using the agar-well diffusion method. The extract concentrations tested are 5%, 10%, 20% and 40%. The result of the antibacterial test of P. scutellarioides (L.) leaf extract with ethanol p.a. as the solvent showed antibacterial activity against E. coli and S. aureus bacteria isolated directly from cow's milk samples with subclinical mastitis at certain concentrations.
Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro Saraswati, Afif Tasya; Sugihartuti, Rahmi; Puspitasari, Yulianna; Witaningrum, Adiana Mutamsari; Rachmawati, Kadek; Maslachah, Lilik; Raharjo, Hartanto Mulyo; Hisyam, Mirza Atikah Madarina; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51106

Abstract

This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.
Effectiveness of the California Mastitis Test (CMT), Reductase Test, and Alcohol Test for Dairy Cows Subclinical Mastitis Detection Eriza Rosalina Rochmah; Dadik Raharjo; Sri Hidanah; Mustofa Helmi Effendi; Adiana Mutamsari Witaningrum; Sunaryo Hadi Warsito
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51443

Abstract

Mastitis is an inflammation of the mammary glands caused by pathogenic bacteria Mastitis divided into 2 types, clinical mastitis which has symptoms of swelling and changes in the shape of the mammary gland, while subclinical mastitis does not indicate clear symptoms. Subclinical mastitis is a very high case in Indonesia, around 97-98%, while clinical mastitis is 2-3%. Effectiveness of the california mastitis test (CMT), reductase test and alcohol test to detect subclinical mastitis in dairy cattle. The number of samples taken was 60 samples of fresh dairy milk from 15 cattles in Medowo Village as much as 50 ml for each sample. The results showed that the test was very effective in identifying subclinical mastitis in dairy cattle. 15% positive results were obtained for subclinical mastitis using CMT, 10% of the milk quality was not good during the reductase test and 18.33% positive with the alcohol test from a total of 60 samples obtained.
Providing black soldier fly maggot flour (Hermetia illucens) as a substitute for concentrated feed for the low density lipoprotein (LDL) and high density lipoprotein (HDL) content of broiler chickens Sisda Indah Millenia Kartika; Sri Hidanah; Adiana Mutamsari Witaningrum; Ratna Damayanti; Widya Paramita Lokapirnasari; Sunaryo Hadi Warsito
Jurnal Agro Veteriner Vol. 8 No. 1 (2024): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i1.64976

Abstract

This study aims to determine the effect of concentrate substitution with maggot black soldier fly flour in the ration on the percentage of Low Density Lipoprotein (LDL) and High Density Lipoprotein (HDL) in broilers. This study used 20 broilers strain Cobb 500 aged five weeks. The P0 group was given a basal diet consisting of 30% concentrate, 60% corn, and 10% rice bran. Groups of P1, P2, P3, and P4 were given basal diet with concentrate substitution of 2, 4, 6, 8% with maggot flour, respectively. Data collection was carried out at the end of the study. Analysis of Variance (ANOVA) showed that the substitution of concentrate with maggot flour from black soldier flies had no significant effect (p>0.05) on the percentage of Low Density Lipoprotein (LDL) and High Density Lipoprotein(HDL)
Co-Authors Achmad Ardianto, Achmad Aurandini, Nailla Ridhani Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Benjamin Christoffel Tehupuring, Benjamin Christoffel Budi Utomo Budiarto Budiarto Budiarto Dadik Raharjo Damayanti , Ratna Dian Ayu Permatasari Dian Ayu Permatasari Dian Yuli Reindrawati Dian Yuli Reindrawati Dwi Wahyu Indriati, Dwi Wahyu Eriza Rosalina Rochmah Estoepangestie, Agnes Theresia Soelih Fauziyah , Nafisah Nurti Habibi, Muhammad Afif Hani Plumeriastuti Hastutiek , Poedji Hisyam, Mirza Atikah Madarina Juniastuti Juniastuti Kadek Rachmawati Khalissa Farah Alifia Kirana, Sabila Citra Koesdarto , Setiawan Kusnoto Kusnoto, Kusnoto Lastuti , Nunuk Dyah Retno Lilik Maslachah M. Qushay, M. Mafruchati, Maslichah Mahfudhah, Dzikra Nasyaya Maria Inge Lusida Masanori Kameoka, Masanori Mega, Toafan Satrio Meilani Meilani, Meilani Mirwa Adiprahara Anggarani Mufasirin Muhammad Qushai Yunifiar M, Muhammad Qushai Mustofa Helmi Effendi Nasronudin Nasronudin Naw, Sin War Nuniek Herdyastuti Permatasari, Dian Ayu Poedji Hastutiek Prihartini Widiyanti Raharjo, Hartanto Mulyo Rahmafitria, Fistara Lesti Rahmi Sugihartuti, Rahmi Ratna Damayanti Rekasni Adallin A/P Morgan A/P Morgan Retno Pudji Rahayu Saraswati, Afif Tasya Sarudji, Suryanie Setiawan Koesdarto Shafina Hanum, Farah Shifa Fauziyah Shuhei Ueda, Shuhei Sisda Indah Millenia Kartika Siti Qamariyah Khairunisa, Siti Qamariyah Sri Hidanah Suherni Susilowati Sumardiko, Dwi Setiani Sunarso, Agus Suprihati , Endang Tacharina, Martia Rani Teguh Hari Sucipto, Teguh Hari Tigor Yeheskiel, Tigor Tita Damayanti Lestari Tomohiro Kotaki, Tomohiro Tri Untari Tri Untari Umi Faricha Bascha Umi Farichah Bascha Utama , Suzanita Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Wibisono, Freshinta Jellia Widya Paramita Lokapirnasari Yulianna Puspitasari