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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Indonesian Journal of Biotechnology Pharmacon Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Paediatrica Indonesiana Jurnal Surya Medika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Majalah Farmaseutik JSFK (Jurnal Sains Farmasi & Klinis) PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Ilmu Kefarmasian Indonesia Jurnal Mandala Pharmacon Indonesia ad-Dawaa : Journal of Pharmaceutical Sciences Indonesian Journal of Hospital Administration JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Ahmad Dahlan Medical Journal Berita Kedokteran Masyarakat Mandala of Health : A Scientific Journal Journal of Clinical Microbiology and Infectious Diseases Indonesian Journal of Biomedicine and Clinical Sciences Indonesian Journal of Pharmacology and Therapy
Titik Nuryastuti
Departemen Mikrobiologi, Fakultas Kedokteran, Kesehatan Masyarakat Dan Keperawatan (FKKMK), Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Jurnal Mandala Pharmacon Indonesia

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Ambroxol Therapy as an Antibiofilm Candidate in Diabetic Ulcer Patients Wahyuddin, Munifah; Sari, Ika Puspita; Asdie, Rizka Humardewayanti; Nuryastuti, Titik
Jurnal Mandala Pharmacon Indonesia Vol. 11 No. 1 (2025): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v11i1.791

Abstract

Patients with diabetic ulcers are susceptible to infection because the tissue in their extremities is constantly exposed to various pathogenic bacteria, so biofilm formation plays an important role in developing the disease. Based on previous studies, ambroxol can inhibit the formation of biofilm bacteria. The purpose of this study was to identify biofilm-forming bacteria, prove that ambroxol has the potential to inhibit and eradicate biofilms formed by bacteria in diabetic ulcers and analyse the potential of ambroxol in eradicating biofilms formed by bacteria in diabetic ulcer patients based on PEDIS and SEM scores. The method used was a 96-well microtiter plate assay (MtPA) with crystal violet and MTT staining techniques, a test using human samples with a quasi-experimental method with sampling carried out by nonprobability sampling. The samples used were diabetic ulcer patients with culture results forming biofilms. The results obtained identified 3 clinical isolates that formed biofilms with 100% Gram-negative bacteria, including two isolates of Pseudomonas aeruginosa and one isolate of Escherichia coli with the ability to form biofilms are moderate. Ambroxol effectively inhibits and eradicates 50% of biofilms formed by diabetic ulcer bacteria at a minimum concentration of 1 mg/ml. Based on the PEDIS score and SEM images of diabetic ulcer patients, there was a decrease in the PEDIS score in each patient, with an average score of 2. Several studies have demonstrated that combining Ambroxol with antimicrobials can synergistically enhance the antibiotic's efficacy against biofilms. The co-administration of Ambroxol with other antimicrobial agents represents a promising approach to improving antibiotic effectiveness. Nevertheless, careful consideration must be given to the concentration of Ambroxol used.
Co-Authors . Mursiti Abu Tholib Aman Ade Christanti Putri Sidabutar Afdina Melya Ganes Febiyanti Aisyah Nur Sapriati Altaufik Ngani Amaia, Devika N. Amalia Setyati Aman, Alia Hanifa Aminy, Sayyidah A. Anggraeni, Nuniek Annisa Aulia Savitri Annisa Somaningtyas Anton Pratama Apriyanto, Muchammad Arief Nurrochmad As, Musdalifa Atikana, Akhirta Atthobari Avanilla Fany Septyasari Bastiaan P. Krom, Bastiaan Bastian P. Krom Cicilia Widhi Astuti Cindy Elvionita Daru Estiningsih Dessy Kurnia Sari Devi Artami Susetiati Dewi Purbaningsih Dhannia Fitratiara Diah Susanti Diyan Ajeng Rossetyowati Diyan Ajeng Rossetyowati Djoko Wahyono Djoko Wahyono Dwi Utami Anjarwati Dwikisworo Setyowireni Edwin W. Daniwijaya Eggi Arguni Elya Antariksana Bachmida Emi Rusdiyati Endang Estriningsih Ervina Damayanti Estiningsih, Daru Estriningsih, Endang Firdhani Satia Primasari Fita Rahmawati Fita Rahmawati Galang Ridha Allatief Hakim, Mohamad Saifudin Hamzah, Hasyrul Hananun Zharfa Hanifah Handi Virawan Hardyanto Soebono Henk J. Busscher Henk J. Busscher, Henk J. Henny C. van der Mei Henny C. van der Mei, Henny C. Hera Nirwati Ika Puspita Sari IKA PUSPITA SARI Ika Puspita Sari Ika Puspita Sari Ika Puspita Sari Ika Puspita Sari Ika Puspita Sari Ika Puspitasari Ika Puspitasari Ika Puspitasari Ika Puspitasari Ilma Tazkiya Iman Iman Ishak SKM., MPH IWAN DWIPRAHASTO Kholidah, Siti Nurhayati Kris Kurniawan Kuwat Triyana LINDA SUKMARINI Linda, Vitia Ajeng Nur Lucky Herawati Luthvia Annisa Marselinus Edwin Widyanto Daniwijaya Maulidia, Faiqoh Nur Maulidiah, Rizka Mawarti, Yuli Meilia Nhadia Amalia Mubasysyir Hasanbasri Mujahidah, Mujahidah Mukhriani, Mukhriani Munifah Wahyuddin Mursiti, Mustofa, Handry Darussalam, Titik Nuryastuti, Mursiti, Mustofa Mustofa Nanang Munif Yasin Ni Luh Putu Vidya Paramita Niken Puspitasari Ning Rintiswati Nunung Yuniarti Nunung Yuniarti Nurkhalika, Rachmi Nurpagino, Bombong Prameshwara, Almarissa Ajeng Praseno Praseno Pratama, Anton Prawarni, Vidyadhari Puspa Prayoga, Muhammad Bagas Pujilestari, Dwi PUSPITA LISDIYANTI Qonita Imma Irfani Rachma Dewi Isnaini Putri Rahmawati, Fita Rakhmat Ari Wibowo Rebriarina Hapsari Retno Wahyuningrum Riandika, Andi Amelia Sari Rina Triasih Ritmaleni, Ritmaleni Rizal Fauzi Rizka Humardewayanti Asdie Rizka Humardewayanti Asdie Rizqi Nurul Khasanah Roel Kuijer Roel Kuijer, Roel SAMUEL BUDI Sapriati, Aisyah Nur Setiawati, Setiawati SHANTI RATNAKOMALA Subagus Wahyuono Supriyati Susi Iravati Sylvia Utami Tunjung Pratiwi Takushi Kaneko Tatang Irianti Tina Amnah Ningsih Titi Ira Pangestuti Tri Murti Andayani Tri Murti Andayani Tri Wibawa Triana Hertiani Tristina Devi Azzahra Untari, Febriana Vera Olfiana Wahyuddin, Munifah Wihda Yanuar Wihda Yanuar Yanuar, Wihda Yeli Trimayanti Yolanda Pitra Kusumadewi Yusrizal Djam’an Saleh ZULLIES IKAWATI