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All Journal Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Veterinary Biomedical and Clinical Journal Jurnal Ilmu Peternakan Terapan Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary
Dadik Rahardjo, Dadik
Veterinary Public Health Departement, The Faculty of Veterinary Medicine, Airlangga University Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : journal of Basic Medical Veterinary

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HISTOPATHOLOGICAL APPEARANCE OF THYMUS ON BROILER UNDER CHRONIC HEAT STRESS Andriani, Ratna; Arimbi, Arimbi; Rahardjo, Dadik; Plumeriastuti, Hani; Legowo, Djoko; Hestianah, Eka Pramyrtha
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19987

Abstract

The aim of this research was to know histopathological change of broiler's thymus which exposed by chronic heat stress. The results revealed that there were negative impact on immune organ (thymus). Broiler that exposed to chronic heat stress showed significantly increased number of heterophil and apoptosis histiocytes and cell depletion. There were 20 broilers that divided into 2 groups, each group consist of 10 broiler. Control group (P0) reared at temperature 24-28oC and humidity 40-55% and Heat Stress group (P1) reared at temperature 36-40oC on the litter housing system at Experimental Animals Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. Before the treatments, broiler had been reared from day-old-chicks to 21-day-old, then continued with heat stress condition from day 22 to 42. Thymus was collected on day 42 to examine histopathological change of thymus and analyzed using Mann-Whitney Test. The results showed significant difference (P<0.05) between Control group and HS (Heat Stress) group by increased number of heterophil, apoptosis histiocytes and cell depletion.
UJI PENETAPAN STABILITAS RETENTION TIME Megestrole acetate DALAM ELUENT MOBILE PHASE MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Cholifah, Siti Chusnul; Lazuardi, Mochamad; Rahardjo, Dadik; Maslachah, Lilik; Sukmanadi, Mohammad; Kurnijasanti, Rochmah
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21093

Abstract

The aim of this research was to determine the level of stability of Megestrole acetate-retention time in storage period for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). The research method used posttest-only control group design by using three treatments and six repetitions. The three repetitions consist into six hours, eight hours and 12 hours. The data were obtained analyzed by Summery Independent T-Test with SPSS 24 for windows. The result showed six hours retention time of Megestrole acetate is stable and eight hours treatment and 12 hours treatment are not stable there is one unstable point of 12 hours treatment that indicates the substance is break down. Based on those result, it could be concluded that the storage time of Megestrole acetate in Eluent Mobile Phase began to show unstable at eight hours of storage.
Acute Toxicity Test Of The Green Viper Snake (Trimeresurus albolabris), Macroscopic Description Of The Kidney And Liver Of Mice (Mus musculus) Darmawan, Moch. Rizky; Rahardjo, Dadik; Tyasningsih, Wiwiek; Kurnijasanti, Rochmah; Legowo, Djoko; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31135

Abstract

According to WHO (2010), the Trimeresurus albolabris snake is a venomous snake with a high number of bite cases in Indonesia. Information on the character and level of toxicity of snake venom in Indonesia, including Trimeresurus spp. snakes, is still minimal. This study aims to determine the LD50 value (lethal dose 50), clinical symptoms, and to determine the macroscopic appearance of the liver and kidneys of mice (Mus musculus) after being injected by a the white-lipped pit viper (Trimeresurus albolabris) venom. The LD50 value of Trimeresurus albolabris venom injected subcutaneously into mice was 5,09 mg/kgBW. Clinical symptoms that appear increased movement activity, increased respiratory rate, lethargy, decreased activity, and reduced grooming. Macroscopic appearance of the liver that looks abnormal is found (1) color changes in the form of pale, pale yellowish, and red spots (petechiae). (2) changes in surface structure found only one liver that is not flat. (3) changes in consistency in the form of brittle easily. In the kidney that looks abnormal, found (1) changes in color in the form of pale. (2) changes in surface structure were only found in one kidney in the form of nodules. (3) changes in consistency in the form of brittle easily and soft.
Isolation and Identification of Bacterial Biosurfactant Activity from Mangrove Sediments Sugihartuti, Rahmi; Pradana, Munawer; Maslachah, Lilik; Chusniati, Sri; Tyaningsih, Wiwiek; Rahardjo, Dadik; Soeharsono, Soeharsono
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.58266

Abstract

Background: Ujung Pangkah mangroves are reported to have been contaminated with heavy metals. Such heavy metals can induce microorganisms to produce biosurfactants. Biosurfactants with amphiphilic characteristics can lower surface tension. Biosurfactants can be used as antibacterial, antifungal, and antiviral for biomedical purposes. Purpose: This study aimed to identify and test the activity of biosurfactant isolates of bacteria from Ujung Pangkah Mangrove sediments, Gresik Regency. Methods: Biosurfactant activity test methods include emulsification index, oil spreading, drop collapse, and parafilm test. Results: The results of the identification of bacterial isolates in this study obtained the genus Bacillus sp. because bacterial isolates show rod shape, Gram-positive, aerobic, and have ellipse-shaped endospores on the subterminal. The results of the biosurfactant activity test with the Emulsification index method showed an average result of 54.39% and the results of the biosurfactant activity test with the oil spreading method showed that there was a clear zone. The average result of the clear zone obtained is 54.83 mm. The average result in the parafilm test was 8.02 mm and the drop collapse test showed positive results characterized by falling and spreading of bacterial isolate fermentation broth supernatants.
Co-Authors Al arif, Mohammad Anam Alpha Fardah Athiyyah Andriani, Ratna Andy Darma Arimbi Aryaloka, Suhita Boedi Setiawan Brillianti, Febby Fairy Bura, Maria Antonia Yersi Dua Cholifah, Siti Chusnul Chusniati, Sri Darmawan, Moch. Rizky de Vries, Garry Cores Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Hani Plumeriastuti Hayuning Nurrodhiya I Gusti Made Reza Gunadi Ranuh IMAM MUSTOFA Indah Norma Triana, Indah Norma Istiana, Izzatul Kadek Rachmawati Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Maghfiroh, Nurutin Tutur Bifatikhatil M Margaretha, Josephira Intan Mirni Lamid Mochamad Lazuardi Mufasirin Munawer Pradana Mustofa Helmi Effendi Nadilla Chrisantya Yusuf Putri Nunuk Dyah Retno Lastuti PA, Deanty Ayu Permatasari, Dian Ayu Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastutiek Pudji Srianto Purbowati, Tri Endah Rahmandari, Dina Agylia Rahmi Sugihartuti, Rahmi Retno Lastuti, Nunuk Dyah Risky Aprillian, Risky Rochmah Kurnijasanti Sabdoningrum, Emy Koestanti Sari, Fifin Kurnia Setiawan Koesdarto Shirakawa, Toshiro Soeharsono Soeharsono Sudarmo, Subijanto Marto Sukmanadi, Mohammad Sunarso, Agus Tatik Hernawati Tjuk Imam Restiadi Tri Suwanti, Lucia Tri Wahyu Suprayogi Trilas Sardjito Tyaningsih, Wiwiek Tyasningsih, Wiwiek Warsito, Sunaryo Hadi Widiyatno, Thomas V. Yulianna Puspitasari Yunus, Muchammad