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All Journal HAYATI Journal of Biosciences Jurnal Sain Veteriner El-Hayah : Jurnal Biologi Journal of Economics, Business, & Accountancy Ventura Biogenesis: Jurnal Ilmiah Biologi Prosiding Seminar Biologi Jurnal Manajemen Teori dan Terapan The Indonesian Biomedical Journal JBMP (Jurnal Bisnis, Manajemen dan Perbankan) BIOEDUKASI SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Biosaintifika: Journal of Biology & Biology Education Majalah Biomorfologi (Biomorphology Journal) Journal of Multidisciplinary Applied Natural Science Berkala Penelitian Hayati Exact Paper In Compilation (EPIC) Sewagati Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Sri Puji Astuti Wahyuningsih, Sri Puji Astuti
Departemen Biologi, Fakultas Sains Dan Teknologi, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
Liposome-based Nanoparticles Encapsulating Vitamin D3 Attenuate IL-6 and TNF-α in a Menopausal Mouse Model Baskara Wiku Adi Kusuma; Sri Ratna Dwiningsih; Ashon As’adi; Sri Puji Astuti Wahyuningsih
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3796

Abstract

BACKGROUND: Vitamin D3 is an essential regulator of immune function, however its bioavailability is limited. Liposomes as nanocarriers can enhance vitamin D3 absorption and delivery, however the application of liposomal vitamin D3 in postmenopausal remains underexplored, particularly in preclinical models. Estrogen deficiency during menopause promotes immune dysregulation and elevates proinflammatory cytokines, including interleukin (IL)-6 and tumor necrosis factor (TNF)-α. This study was conducted to evaluate the effects of liposomal vitamin D3 supplementation on serum vitamin D3, IL-6, and TNF-α levels in an ovariectomy-induced menopausal mouse model.METHODS: Mice were randomly divided into four groups comprising non-surgical control (N), ovariectomized without treatment (D−), conventional vitamin D3-treated (D+), and liposomal vitamin D3-treated (LD). Treatments were administered daily via oral gavage for two months. Serum vitamin D3, IL-6, and TNF-α levels were measured by enzyme-linked immunosorbent assay (ELISA). IL-6 and TNF-α data were analyzed by ANOVA with Duncan’s post-hoc test, while vitamin D3 data were analyzed using the Brown-Forsythe test with Games-Howell post-hoc test (p<0.01).RESULTS: Ovariectomy significantly decreased vitamin D3 levels and increased IL-6 and TNF-α levels in the D− group. Conventional vitamin D3 supplementation (D+) significantly decreased serum vitamin D3 levels and slightly decreased IL-6 and TNF-α levels. Liposomal vitamin D3 (LD3) significantly increased vitamin D3 levels and decreased TNF-α, only slightly decreasing IL-6. Correlation analysis showed a negative association between serum vitamin D3 levels and both cytokines.CONCLUSION: Administration of vitamin D3 liposomes was able to increase vitamin D3 levels and suppress IL-6 and TNF-α towards normal levels. LD3 offers enhanced bioavailability and anti-inflammatory effects, making it a promising therapeutic strategy for managing menopause-associated inflammation and related systemic disorders.KEYWORDS: menopause, liposomal VD3, inflammation, IL-6, TNF-α
Antioxidant and Anticancer Effects of Red Okra (Abelmoschus esculentus L.) Ethanol Extract through In Vitro and In Vivo Colorectal Cancer Models Wahyuningsih, Sri Puji Astuti; Mamuaya, Brigita Klara Krisdina; Dewi, Firli Rahmah Primula; Hapsari, Lukiteswari Dyah Tri; Kusuma, Baskara Wiku Adi; Nurhayati, Awik Puji Dyah
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.367

Abstract

Colorectal cancer (CRC) remains the second leading cause of cancer-related mortality worldwide. Affordable herbal sources such as red okra (Abelmoschus esculentus L.) pods have gained attention as potential alternative therapies for CRC. This study aimed to evaluate the antioxidant and anticancer effects of red okra ethanol extract (ROE) using both in vitro and in vivo colorectal cancer models. The antioxidant activity of ROE was assessed using the DPPH assay, while cytotoxic activity was evaluated using the MTT assay on SW480 and HCT116 cell lines. An in vivo study was conducted using rats divided into six groups: normal control, negative control (MNU 10 mg/kg BW), positive control (MNU + methotrexate 0.08 mg/kg BW), and treatment groups receiving MNU combined with ROE at doses of 50, 100, and 200 mg/kg BW for 28 days. Serum levels of Bcl-2, COX-2, VEGF, and MMP-9 were analyzed, and histopathological evaluations of colon tissues were performed. Data were statistically analyzed using one-way ANOVA followed by Duncan’s post hoc test. Statistical significance was determined at p<0.05. ROE exhibited potent antioxidant activity (IC₅₀ = 59.66 ppm) and induced cytotoxic effects by reducing SW480 cell growth and inhibiting HCT116 cell proliferation. Moreover, ROE significantly decreased the expression of Bcl-2, COX-2, VEGF, and MMP-9. These biomarkers are associated with apoptosis inhibition, angiogenesis, inflammation, and metastasis, respectively. Histopathological analysis confirming reduced inflammatory infiltration and suppression of colon carcinogenesis. The optimal in vivo dose was 50 mg/kg BW. These findings support the development of ROE as a promising natural agent for colorectal cancer prevention and therapy.
Normative community pressure in brand community: a study of young entrepreneurs community of mandiri bank Lindiawati Lindiawati; Indrianawati Usman; Sri Wahyuni Astuti
Journal of Economics, Business, and Accountancy Ventura Vol. 22 No. 1 (2019): April - July 2019
Publisher : Universitas Hayam Wuruk Perbanas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14414/jebav.v22i1.1700

Abstract

Brand community has been extensively built and developed by either the companies or the customers voluntarily. These two gorups with different perspective hold different purposes. For the companies, brand community is considered an effective marketing strategy which is expected to achieve customer brand engagement. Anyhow, often companies focus more on the purposes of the brand community, but do not be quite aware of the policies, rules, or traditions firstly created as norms in order to lead the way the community members perceive and act. Often norms become pressures for the brand community members causing them reluctant to engage with he community. Referring to the theory of relationship marketing, this study develops a model of normative community pressure that is affected by both brand community identification and inter-member relationship quality, that are previously affected by brand relationship quality. The empirical analysis derives primary data of young entrepreneurship community created by one of the big five banks in Indonesia quantitatively analyzed using Structural Equation Modeling (Warp-PLS). The result helps explain the antecedents causing normative community pressure of the brand community. The implication of this study is that communitfy setter should regularly identify and analyze rules or practices within the community that might be potential barrier for the members in building engagement with the community.
Potensi Ekstrak Ganoderma applanatum Terhadap Kadar MDA dan Bax pada Mencit (Mus musculus) yang Terinduksi Diethylnitrosamine Siti Rahayu; Win Darmanto; Sri Puji Astuti Wahyuningsih
Exact Papers in Compilation (EPiC) Vol. 7 No. 2 (2025): Mei 2025
Publisher : Lembaga Penelitian Pengabdian Masyarakat Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/epic.v7i2.1514

Abstract

Polisakarida Ganoderma applanatum memiliki sifat antioksidan sebagai respon biologis yang kuat untuk terapi kanker. Penelitian ini menguji potensi ekstrak polisakarida G. applanatum terhadap kadar MDA dan Bax pada tikus (Mus musculus) yang diinduksi DEN. Tikus betina galur DDY dibagi secara acak menjadi 4 kelompok (n=6). DEN diberikan secara intraperitoneal dua kali seminggu, kemudian doxorubicin sebagai kontrol positif dan ekstrak polisakarida sebagai perlakuan selama satu bulan secara oral. Metode penelitian dilakukan dengan membandingkan KN yang diberi air; K- yang diberi DEN 100 mg/kg BB; K+ yang diberi DEN 100 mg/kg BB dan doksorubisin 10 mg/kg BB; dan P yang diberi DEN 100 mg/kg BB dan ekstrak polisakarida G. applanatum 150 mg/kg BB. Dari analisis statistik uji ANOVA yang dilanjutkan dengan uji Duncan, diperoleh bahwa pemberian ekstrak polisakarida G. applanatum dibandingkan dengan K- tidak menunjukkan perbedaan bermakna kadar MDA dan Bax. Data hasil percobaan sebagai berikut: KN menunjukkan kadar MDA sebesar 1,62±0,28 nmol/mL dan Bax 4,41±2,14 ng/mL. Kadar MDA K- sebesar 1,58±0,11 nmol/m dan Bax 3,91±1,84 ng/mL. Kadar MDA K+ sebesar 1,75±0,21 nmol/mL dan Bax 4,30±1,61 ng/mL. Kadar MDA P sebesar 1,74±0,29 nmol/mL dan Bax 2,28±1,27 ng/mL.
Co-Authors Achhlam, Divany Hunaimatul Adelah Putri Milenia Sari Ahmar, Rasyidah Fauzia Aini Nurul Alfiah Hayati Almando Geraldi Amalia Fachrisa Amalia Nur Mashita Andita Ayu Mandasari Anjar Tri Wibowo Ashon As’adi Awik Puji Dyah Nurhayati Bachruddin, Muhammad Bambang Irawan Baskara Wiku Adi Kusuma Baskara Wiku Adi Kusuma Dewi Rahmawati Dita Wahyunita Do, Hoang Dang Khoa Dwi Kusuma Wahyuni Dwi Winarni Fachrisa, Amalia Fatimah Firli Rahmah Primula Dewi Hapsari, Lukiteswari Dyah Tri Hari Soepriandono Hastari Wuryastuti Himawan Sardju I Gede Widhiantara I Made Wisnu Adhi Putra Indri Safitri Mukono, Indri Safitri Indrianawati Usman Intan Ayu Pratiwi, Intan Ayu Intan Permata Putri Intan Permata Putri ISTUNING MA’UNAH Junairiah Junairiah Kamiso H.N. Kushendarsasi Kusuma Eko Purwantari Kusuma, Baskara Wiku Adi Lindiawati Lindiawati Listijani Suhargo, Listijani Lukiteswari Dyah Tri Hapsari Mamuaya, Brigita Klara Krisdina Manikya Pramudya Mochammad Aqilah Herdiansyah Muhammad Hilman Fu'adil Amin Na'ilah Insani Alifiyah Nabilatun Nisa’ Nadia Shoukat Nadyatul Ilma Indah Savira NI KADEK YUNITA SARI Nisa’, Nabilatun Novaria Sari Dewi Panjaitan Permatasari, Anak Agung Ayu Putri Prenggono, Muhamad Darwin Putri, Salsabilla Wiyana Putu Angga Wiradana R. Warsito Rasyidah Fauzia Ahmar Rasyidah Fauzia Ahmar S. Sugiharto Sapto Andriyono Shoukat, Nadia Siti Rahayu Sri Ratna Dwiningsih Sugiharto Suhendra Agung Wibowo Umi Nur Alfiah Ummi Zubaidah Virid Gibson, Virid Vuanghao Lim Vuanghao Lim Win Darmanto