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Journal : BUANA SAINS

KEMAMPUAN Bacillus subtilis dan Pseudomonas fluorescens DALAM MELARUTKAN FOSFAT DAN MEMPRODUKSI HORMON IAA (Indole Acetic Acid) UNTUK MENINGKATKAN PERTUMBUHAN TANAMAN TOMAT Istiqomah Istiqomah; Luqman Qurata Aini; Abdul Latief Abadi
BUANA SAINS Vol 17, No 1 (2017)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.403 KB) | DOI: 10.33366/bs.v17i1.580

Abstract

Tomato is an important horticultural commodity in Indonesia and plays strategic role in fulfilling daily needs of the public. The increase in production of tomato can be done by optimizing the provision of nutrients that support growth. The ability of bacteria as biological fertilizer to dissolve phosphate and produce IAA (Indole Acetic Acid) are important criteria for the use of bacteria as agents of plant growth promoters. The purpose of this study was to determine the ability of some isolates of Bacillus subtilis and Pseudomonas fluorescens in dissolving phosphate and produce IAA to enhance the growth of tomato. Phosphate test results showed that all isolates of B. subtilis and P. fluorescens capable of dissolving phosphate with the highest value indicated by P. fluorescens UB-PF5 (18 mm). All isolates of bacteria capable to produce IAA. The resulting value IAA ranged from 0.69 to 1.09 ppm with the highest value produced by B. subtilis UB-ABS2 (1.09 ppm). The test results on the parameters of root weight, root length, plant height and number of leaves showed that tomato treated with B. subtilis and P. fluorescens increased significant growth compared with watering only with distilled water. The highest increased root weight and root length were resulted by P. fluorescens UB-PF5 with values 97.10% and 64.83%, respectively. The highest increased plant height and number of leaves length were resulted by P. fluorescens UB-PF6 with values 38,80% and 30,7%, respectively.
KEMAMPUAN DUA SPESIES Enterobacter sp. SEBAGAI BAKTERI PEMACU PERTUMBUHAN TANAMAN KENTANG (Solanum tuberosum L.) PADA KONDISI CEKAMAN KEKERINGAN Khoirun Enisa Maharina; Nurul Aini; Luqman Qurata Aini
BUANA SAINS Vol 17, No 1 (2017)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.664 KB) | DOI: 10.33366/bs.v17i1.581

Abstract

Enterobacter xiangfangensis and Enterobacter cloaceae are known to have the ability as a phosfat solubilizing and producing hormone IAA. Their also have ability to survive under drought stress in vitro assay conditions. Both of these bacteria has good potential as a potato plant growth promoting bacteria, especially in drought stress conditions. This is an alternative to improve tolerance and growth of the potato crop in drought stress conditions. The results showed that the level of drought stress 75% of field capacity can boost the potential of endophytic bacteria E. xiangfangensis in increasing leaf area, plant dry weight, root length and physiological activity i.e total phosphorus content in potato plants. Endophytic bacteria E. xiangfangensis is better to increase potato plant height than endophytic bacteria E. cloaceae. These results are supported by the results of potential test of E. xiangfangensis which have the advantage of producing hormones IAA compared with E. cloaceae. Furthermore, in drought stress conditions 50% of field capacity and 75% of field capacity have different effects on the observations of root length growth parameters potato plants.
POTENTIAL ENTOMOPATHOGENIC BACTERIA ISOLATED FROM EXTREME CONDITION AREA SIDOARJO MUD, INDONESIA AGAINST Spodoptera litura FAB (LEPIDOPTERA: NOCTUIDAE) Tita Widjayanti; Luqman Qurata Aini; Restu Rizkyta Kusuma; Istiqomah Istiqomah
BUANA SAINS Vol 23, No 1 (2023)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/bs.v23i1.4554

Abstract

Biological control by using entomopathogenic bacteria shows potential to be developed as an alternative technique to control Spodoptera litura. Bacteria that can live in extreme environments are reported to have high efficiency and the ability to survive in various environmental conditions, such as in the area of Sidoarjo mud. Related to the issue, this research aimed to find out bacteria that can survive in extreme conditions and are potentially entomopathogenic to control S. litura. The study was conducted from January until July 2021 in the Sidoarjo mud area and laboratory of Biological Control, Faculty of Agriculture, University of Brawijaya. The research was conducted using methods including consist of a sampling of Sidoarjo hot mud, isolation, and screening of bacteria that is potential as entomopathogenic bacteria, bioassay of mortality S. litura, development of larvae and pupal using Completely Randomized Design with 11 treatments and 4 replications, and molecular identification by 16S rRNA. Data for mortality and development of larvae and pupal were submitted to variance analysis, followed in comparison to the averages of the Duncan test at a 5% level of significance. The results showed that 43 colony bacteria from Sidoarjo hot mud have been successfully isolated and obtained 9 isolates selected as entomopathogenic bacteria against S. litura with a percentage of mortality larvae reached 60%. Moreover, bacteria have the potential to inhibit the development of larvae and pupal S. litura. Molecular identification showed that potential isolates are Bacillus subtilis strain 15A-B92, Bacillus thuringiensis strain GTG-29, and Bacillus anthracis strain BA1035.
Co-Authors Abd Latif Abadi Abd Latif Abadi Abdul Cholil Abdul Cholil Abdul Karim Fanani Abdul Latief Abadi Abdul Latief Abadi Abdul Latief Abadi Achmad Noerkhaerin Putra Achmad Roekhan Adi Setiawan Agung Nugroho Agung Wahyu Susilo Aini Nurul Aini, Febrilia Nur Akhmad Rizali Albert Fernando Sitorus Alda Dwi Aprilia Alfi Inayati Aminudin Afandhi Aminudin Afandhi Anastasya, Nabilla Alya Andhy Handoko Andhy Handoko Anton Muhibuddin Apriyani, Aliffia Nuraita Ardiyan Taruna Arifah, Fitri Ariffin Noor Sugiharto Arifin Noor Sugiharto Aris Budiman Asnawi Asnawi Asparita, Yulia Dwi Auliya Zendhabad Aulya Retno Setyari Aulya Retno Setyari Bayu Setyawan, Bayu Berliana, Sonia Bogi Diyansah Bosha, Mohammed Cindy Diah Ayu Fitriana Cokorda Javandira Cokorda Javandira Daniel Happy Putra Dayanti, Ayu Ike Dewi, Fibrianti Shinta Dian Eka Kusumawati Dianti, Sylvia Dianti, Sylvia Herli Dinata, Gallyndra Fatkhu Diyana Lestari Dwi Wulandari Dyah Ayu Kusuma Wardani Eka Kartini Eriyanto Yusnawan Eriyanto Yusnawan Fibrianti Shinta Dewi Firdausi, Wita Galuh Setyanto Pradana Galuh Setyanto Pradana Ganestya Indina Sari Ganestya Indina Sari Hagus Tarno Hapsari, Femita Hasanah, Ifa Maulidah Husna Fikriya Baroroh I Made Indra Agastya Ichsan Firmansyah Imam Chanif Imam Chanif, Imam Indah Anita Sari Irisa Trianti Irisa Trianti Ismat Ishak Istiqomah Istiqomah Istiqomah, Istiqomah Ito Fernando Ito, Satoshi Iva Dewi Lestariningsih, Iva Dewi Izza, Junda Fauzul Karuniawan Puji Wicaksono Kestrilia Rega Prilianti Khoirun Enisa Maharina Khoirun Enisa Maharina Khoirunisa, Fenti Rahma Kusuma, Restu Rizkyta Liliek Sulistyowati Lilis Suryani Ma'alifah, Nur Maharina, Khoirun Enisa Minal Maimanah Mintarto Martosudiro Mochammad Syamsul Hadi Mochammad Syamsul Hadi Muhammad Akhid Syib’li Muhammad Akhid Syibli Muhammad Febriansyah Muhidin, Ahmad Nava Karina Nindi Kurniasari Novia Dwirani Novie Utami Asputri Nugroho Sulistyo Putro Nugroho Sulistyo Putro Nur Azizah Nurul Aini Nurul Aini Nurul Aini Nurul Aini Oktaviani, Rahmania Pamungkas, Bayu Aji Paramyta Nila Permanasari, Paramyta Nila Prabowo, Matheus Randy Prillianti, Kestrillia Rega Puput Wahyuningsih Rachmi Masnilah Rahmadani, Pradina Rahman, Fakih Nur Ramadhan, Varotama Putra Ramadhani, Fatchuliani Safitri Rania, Khansa Diva Ratri Kusuma Devi Ratri Kusuma Devi Rida Iswati Rifani Rusiana Dewi Rina Rachmawati Rina Rachmawati Roekhan, Achmad Rudin, Sofyan Sudirman Saitama, Akbar Sallygresya Theodora Dwifelita Matondang Santoso, Verisca Agil Winanda Sektiono, Antok Wahyu Serdani, Army Dita Setyono Yudo Tyasmoro Sheila Desi Kharisma Sheila Desi Kharisma Silfiani, Intan Siska Irhamnawati Pulogu Soemarno Soemarno Suharjono Sukmarani, Elok Syamsuddin Djauhari Syamsul Arifin Syarifah Maulidya Rahmah Syib'li, Muhammad Akhid Syibli, Muhammad Akhi Syib`li, Muhammad Akhid Syib’li, Muhammad Akhid Taruna, Ardiyan Tatiek Wardiyati Theresia Rani Kartika Ayu Tita Widjayanti Toto Himawan Toto Himawan Tri Ardiyati Tri Ardiyati Tri Ardyati Trisnawati, Luh Putu Adi Tutung Hadi Astono Tutung Hadiastono Tutung Hadiastono Udayana, Cicik Uma Khumairoh Wardiyati, Tatiek Wita Firdausi Wiwik Jatnika Wiwin Sumiya Dwi Yamika Wiwin Sumiya Dwi Yamika Wiwin Sumiya Dwi Yamika Wiwit Wicaksono Jati Yani Kurniawan, Yani Yogo Setiawan Zainudin Zainudin Zainudin Zainudin Zainul Abidin