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Penetapan kadar fenolik total ekstrak kayu raru (Cotylelobium lanceolatum Craib) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri UV-Vis Lastri Afni; Anny Sartika Daulay; Ridwanto Ridwanto; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.306

Abstract

Many Indonesian people use or use plants as traditional medicine; one plant that has many benefits is raru bark (Cotylelobium lanceolatum Craib), which is used as a traditional medicine in various diseases such as diarrhea, malaria, and diabetes. This study aims to find out what classes of compounds are found in raru bark extract and to determine the value of total phenolic levels contained in raru bark extract in various concentrations (96%, 70%, 50%). The stages of this research include processing plant materials, making raru bark ethanol extract, characterization examination, phytochemical screening, and determining total phenolic levels of raru bark extract based on differences in ethanol concentration by UV-Vis spectrophotometry method. The results showed that raru bark ethanol extract contains compounds, flavonoids, tannins, saponins, steroids, and phenolics. Determination of total phenolic content by determining the maximum wavelength of gallic acid and calculation of entire phenolic content by UV-Vis spectrophotometry method. Results of selecting the whole phenolic content of raru bark ethanol extract at a concentration of 96%; 28,6544±0,1548 mgGAE/g, 70%; 23,9682±0,1270 mgGAE/g, 50%; 20,5825±0,1993 mgGAE/g, then the phenolic content that best produces total phenolic levels is raru bark ethanol extract (Cotylelobium lanceolatum Craib) concentration of 96%.
Skrining fitokimia dan isolasi senyawa steroid/triterpenoid dari ekstrak n-heksana daun Pepaya (Carrica papaya L) Cindy Widya Rosa Br Simarmata; Haris Munandar Nasution; M Pandapotan Nasution; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.324

Abstract

In Indonesia, papaya plants are common in several areas, from Sabang to Merauke. As a tropical country, almost all corners of Indonesia have various shapes and different types of papayas. Papaya leaves (Carica papaya L.) contain tannins, alkaloids, flavonoids, terpenoids, and saponins which act as antiparasitics. This research aims to determine the results of the chemical compound groups found in papaya leaves (Carica papaya L.). To determine the characteristics of isolates from n-hexane extract of papaya leaves (Carica papaya L.) using UV and IR spectrophotometry. This research includes making simplicia from papaya leaves, then phytochemical screening, then simplicia characterization, then making extracts from papaya leaf simplicia, after that analysis of the extract using the thin layer chromatography (TLC) method, then isolation using column chromatography (KK), purity testing using Two-way thin layer chromatography (TLC) and the isolation obtained were identified using ultraviolet (UV) spectrophotometry and infrared (IR) spectrophotometry. The results of the characterization of the Carica papaya L Simplicia showed that the water content was 4%, the water-soluble essence content was 16.64%, the ethanol-soluble essence content was 11.6%, the total ash content was 9.88%, the ash content was 0. 66%, the results of the phytochemical screening of Carica papaya L., simplicial contain steroid/triterpenoid compounds, alkaloids, flavonoids, saponins, tannins. The results of the isolated analysis showed that the isolate obtained was a violet-colored steroid/triterpenoid compound. The isolate obtained was analyzed using UV light spectrophotometry, giving an absorbance at a wavelength of 207 nm, indicating that there was a chromophore group. The IR spectrophotometry results showed OH, aliphatic CH, and CH2 groups, CH3, C=O and CO.
Prevalensi bakteri Salmonella sp. pada daging ayam potong di pasar tradisional, pasar modern, dan merek terkenal di kota Medan. Lulu Ilma Khoirun Nissa; Yayuk Putri Rahayu; D. Elysa Putri Mambang; Anny Sartika Daulay
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.330

Abstract

Chicken is a source of animal protein for humans. Chicken meat generally contains unsaturated fat; chicken meat also has a compact meat texture and simple protein, so it is easily digested. However, chicken meat can also be contaminated with Salmonella sp. Pathogenic bacteria can cause typhus or typhoid fever, caused by traders' lack of cleanliness and hygiene or their environment. Research was conducted to identify the presence of Salmonella sp. Pathogenic bacteria and analysing the value of Salmonella sp. Pathogenic bacteria contamination that met the Indonesian National Standard (SNI), namely harmful/25g in chicken meat from traditional markets, modern markets, and well-known brands in Medan City, and to determine the prevalence value of Salmonella sp. Bacteria. The method of research uses Total Plate Count (TPC). An identification test is first carried out to calculate and determine the presence of bacteria in a test sample for the existence of Salmonella sp. Pathogenic bacteria and choose the total value of contaminants using a Total Plate Count (TPC), then identify using a gram staining test. These biochemical tests include the indole test, MRVP test, citrate test, urea test, TSIA test, and LIA test. Then, the last one determines the Prevalence value of Salmonella sp. Bacteria. The results showed that the samples of traditional market and modern market chicken meat were positive for the presence of the pathogenic bacteria Salmonella sp. by obtaining Subgenus Salmonella Typhimurium on N2H8 sample code APTL and APTB, N3H15 sample code APTL, APTB, APMI and obtaining Subgenus Salmonella Thypi on N3H15 sample code APMS and the contamination value of the pathogenic bacteria Salmonella sp. Exceeds the threshold and does not meet the Indonesian National Standard (SNI), namely negative/25g. The prevalence value obtained by the pathogenic bacteria Salmonella sp. in broiler meat at Traditional Markets, Modern Markets, and well-known brands is 75%, 50%, and 0%, respectively.
Skrining fitokimia dan isolasi senyawa steroid/triterpenoid dari ekstrak n-heksana biji nangka (Artocarpus heterophyllus Lam). Tawir Rina Rezeki; Haris Munandar Nasution; M Pandapotan Nasution; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.331

Abstract

Nangka (Artocarpus heterophyllus Lam) merupakan tumbuhan yang dapat berkembang dengan baik di wilayah tropis seperti Indonesia. Biji Nangka mengandung senyawa kimia jenis steroid/triterpenoid, termasuk beberapa jenis steroid yang memiliki manfaat sebagai bahan dalam obat-obatan, seperti estrogen, progestin, dan kardenolida. Penelitian bertujuan untuk melakukan pengujian secara fitokimian dan juga mengisolasi senyawa steroid/ triterpenoid biji Nangka. Penelitian ini menggunakan metode eksperimental, termasuk proses ekstraksi senyawa dengan metode maserasi dan isolasi senyawa aktif melalui kromatografi kolom. Identifikasi isolat murni dilakukan dengan menggunakan spektrofotometri UV dan spektrofotometri IR. Hasil skrining fitokimia menunjukkan keberadaan senyawa steroid/triterpenoid, glikosida, dan alkaloida dalam sampel. Isolat tersebut memiliki warna ungu dengan nilai RF 0,25, dan puncak absorbansi maksimum terletak pada panjang gelombang 208,20 nm, menunjukkan adanya gugus kromofor. Hasil spektrofotometri IR menunjukkan adanya gugus seperti OH, -CH alifatis, CH2, CH3, C=O, C-O, dan C=C. Kesimpulan dari penelitian ini adalah bahwa isolat yang diperoleh termasuk dalam golongan senyawa steroid/triterpenoid.  
Uji aktivitas antibakteri formulasi sediaan gel antijerawat ekstrak etanol kulit buah alpukat (Persea americana Mill) terhadap bakteri Staphylococcus aureus Novi Yuliandari; Yayuk Putri Rahayu; Minda Sari Lubis; Rafita Yuniarti
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.327

Abstract

The avocado peel contains flavonoids, saponins, tannins, steroids, and glycosides and has antimicrobial properties. Staphylococcus aureus is a gram-positive bacteria and typical skin microorganism that causes acne. It produces lipase, which breaks down free fatty acids from skin lipids. The gel preparation was chosen. It is better used in treating acne because it does not contain oil or affect acne. This study aimed to determine whether the ethanol extract of avocado peel can be used as an anti-acne gel and to choose the inhibitory power of the gel preparation against Staphylococcus aureus. The ethanol extract of avocado peel was prepared using the percolation method; the extract was formulated into anti-acne gel with 10%, 20%, and 30%. Then, a physical evaluation of the preparation was carried out by assessing organoleptic, homogeneity, pH, viscosity, adhesion, spreadability, irritation, skin moisture, and syneresis. Then, the activity of Staphylococcus aureus bacteria was tested on gel preparations. The result is that the ethanol extract of avocado peel can be formulated into gel preparation. It has a distinctive aroma, is homogeneous, has a pH range of 6,2-6,9, has a value of viscosity, spreadability, and good adhesion, does not irritate, can moisturize the skin, and does not cause syneresis. The gel preparation had antibacterial activity against Staphylococcus aureus, namely a concentration of 10% inhibition of 14.56 mm, 20% inhibition of 19.46 mm, and 30% inhibition of 21.2%, so the interpretation of the inhibition zone was included in the susceptible category.
SOSIALISASI PEMANFAATAN YOGURT UNTUK MENURUNKAN HIPERTENSI KEPADA MITRA PENGELOLA ZAKAT YAYASAN PENDIDIKAN AMALIYAH KECAMATAN MEDAN TEMBUNG Yayuk Putri Rahayu; Haris Munandar Nasution Nasution; Supiyani Supiyani
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 4 No. 5 (2023): Volume 4 Nomor 5 Tahun 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v4i5.21628

Abstract

Yogurt diketahui dapat menurunkan hipertensi dikarenakan dalam yogurt mengandung bakteri probiotik yang dapat mendorong pelepasan protein yang dapat menurunkan tekanan darah. Tujuan kegiatan PKM ini adalah memberikan sosialisasi pengetahuan tentang pemanfaatan yogurt untuk menurunkan hipertensi kepada Mitra Pengelola Zakat (MPZ) Yayasan Pendidikan Amaliyah kelompok forum silaturahmi Badan Kemakmuran Mesjid (BKM) Al-Hikmah kecamatan Medan Tembung. Metode yang digunakan dalam PKM ini adalah: (1) Penyuluhan (sosialisasi) ceramah pentingnya yogurt untuk menurunkan hipertensi, dan (2) Pemberian pengetahuan berbagai jenis contoh produk yogurt. Kegiatan PKM dilakukan dengan beberapa tahap: (1) Tahap survey, melakukan kerjasama dengan mitra, dan menentukan tema kegiatan; (2) Tahap persiapan PKM; (3) Tahap penyuluhan dan sosialisasi, dan penyampaian materi; dan (4) Tahap penyampaian berbagai contoh jenis produk yogurt. Hasil yang didapatkan dari kegiatan PKM ini adalah pengetahuan mitra peserta PKM tentang yogurt dapat menurunkan hipertensi meningkat menjadi 71.21% dari rata-rata nilai pengetahuan 4.40 menjadi 7.53. Kesimpulan dari kegiatan pengabdian PKM ini adalah (1) Peserta mitra menjadi mendapatkan pengetahuan tentang yogurt dapat menurunkan hipertensi, dan (2) Peserta mitra mendapatkan pengetahuan berbagai contoh jenis yogurt. Kegiatan PKM yang telah dilaksanakan dapat meningkatkan pengetahuan masyarakat sehingga dapat meningkatkan kualitas hidup dari segi kesehatan di keluarga dan masyarakat.
PEMBERDAYAAN MASYARAKAT MELALUI EDUKASI PENGOLAHAN PROPOLIS KELOMPOK SAPO BEN BEN DESA BATU JONG JONG KECAMATAN BAHOROK Yayuk Yuliana; Yayuk Putri Rahayu; Diana Sopha; Muhammad Arif; Oca Putri Nazuhra
E-Amal: Jurnal Pengabdian Kepada Masyarakat Vol 4 No 3: September-Desember 2024
Publisher : LP2M STP Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47492/eamal.v4i3.3512

Abstract

The problem faced by the people of Batu Jong Jong village is the lack of knowledge about the use of bee glue or what is known as raw propolis. The aim of this service is to make it easier for people to process raw propolis, which is something that needs to be understood and understood. The method used in this service is by conducting education or education, surveys by administering questionnaires for pre-test and post-test to measure initial abilities, level of understanding of knowledge about propolis, providing socialization and training. Results: 85.71% of the village community already understands the knowledge about the use of propolis processing as a potential natural resource in Batu Jong Jong village, Bahorok District
ANTIBACTERIAL TEST OF ETHANOLIC EXTRACT NANOPARTICLES FROM ARUM MANIS MANGO LEAVES (MANGIFERA INDICA L. VAR. ARUM MANIS) AGAINST STAPHYLOCOCCUS AUREUS Lubis, Nadya Frestika; Rahayu, Yayuk Putri; Nasution, Haris Munandar; Lubis, Minda Sari
Jurnal FARMASIMED (JFM) Vol 5 No 2 (2023): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v5i2.1687

Abstract

Secondary metabolites with antibacterial properties have been found in arummanis mango leaves. Alkaloids, flavonoids, tannins, saponins, triterpenoids, and steroids, especially the mangiferin molecule found in several mango varieties, are also present in arum manis mango leaf extract. Mangiferin has shown action on Staphylococcus aureus as an antibacterial agent that can prevent the development of bacteria. Staphylococcus aureus is one of the bacteria that triggers skin infections. In this research, extract nanoparticles and inhibition tests against staphylococcus aureus bacteria were prepared using extract nanoparticles and ethanol extract of arum manis mango leaves. The manufacture of nanoparticles in this study was carried out because smaller and nanometer-sized materials have more special chemical and glass characteristics; their small size has a greater surface area to volume ratio than larger particles. Nanoparticles are small particles ranging in size from 1 to 1000 nm. A particle size analyzer is used to characterize nanoparticles. Using the disc diffusion method, antibacterial activity tests were carried out at concentrations of 25%, 50%, and 75%, using nanoparticle extracts, and 2.5%, 5%, and 7.5% for extract concentration. The results of the extract nanoparticle size characterization were 40.2 nm. The bacterial inhibition zones of arum manis mango leaf extract obtained at concentrations of 25%, 50%, and 75% were 11.9, 14.4, and 15.4 mm, respectively. Meanwhile, the inhibition zones of nanoparticle bacteria extract 2.5%, 5%, and 7.5% were 15.5, 17.5, and 18.3 mm, respectively. The concentration of 2.5% nanoparticle extract has the inhibition power of bacteria equivalent to that of 75% ethanol extract, so it says that the nanoparticles can reduce the size dose and have an inhibition power in the moderate sensitivity category.
SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MATOA (Pometia pinnata J.R. FORST & G. FORST) TERHADAP PERTUMBUHAN BAKTERI Salmonella thypi Gultom, Syarifah Elena; Mambang, D. Elysa Putri; Nasution, Haris Munandar; Rahayu, Yayuk Putri
Jurnal Farmasi Klinik dan Sains Vol 3, No 1 (2023): Jurnal Farmasi Klinik dan Sains
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Gombong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jfks.v3i1.1062

Abstract

Matoa memiliki sejarah panjang dalam penggunaan obat tradisional (Pometia pinnata J.R. Forst & G. Forst). Antioksidan termasuk vitamin C dan E, serta flavonoid, tanin, dan saponin dapat ditemukan dalam jumlah yang melimpah pada buah matoa. Penelitian ini bertujuan untuk mengetahui sifat antibakteri dari ekstrak etanol serbuk simplisia daun matoa dan metabolit sekundernya terhadap Salmonella thypi. Variabel bebas dalam penelitian ini adalah ekstrak etanol daun matoa (Pometia pinnata J.R. Forst & G. Forst). Pengujian dilakukan pada ekstrak 50%, 30%, dan 30%. Aktivitas antibakteri ekstrak etanol daun matoa ditentukan dari kemampuannya dalam menghambat pertumbuhan Salmonella typhi. Pelarut etanol digunakan untuk menguapkan ekstrak kental yang diperoleh dari hasil maserasi daun matoa. Ekstrak daun matoa diencerkan dengan DMSO 30%, 40%, dan 50%. Pengenceran tersebut akan menentukan seberapa baik ketahanan Salmonella typhi terhadap antibiotik. Metabolit sekunder seperti flavonoid, tanin, alkaloid, saponin, dan steroid dianalisis pada ekstrak etanol daun matoa dan simplisia. Salmonella thypi dihambat dengan rata-rata 12,6 mm pada konsentrasi 30% (Interpretasi menengah), 13,8 mm pada konsentrasi 40%, dan 15,3 mm pada konsentrasi 50% dengan menggunakan ekstrak etanol daun matoa. Aktivitas antibakteri dari ekstrak etanol daun matoa ditunjukkan dengan zona hambat dengan diameter rata-rata daerah hambat yang sesuai dengan interpretasi Intermediate (I). 
ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI BAKTERI ASAM LAKTAT PADA MINUMAN CLASSIC ENZYME BERBAGAI MACAM BUAH TERHADAP PERTUMBUHAN BAKTERI STAPHYLOCOCCUS AUREUS Harahap, Siti Salimah; Ridwanto, Ridwanto; Daulay, Anny Sartika; Rahayu, Yayuk Putri
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 8 No. 3 (2024): DESEMBER 2024
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v8i3.32917

Abstract

Classic Enzyme adalah cairan yang dihasilkan dari buah-buahan melalui proses fermentasi dengan waktu panen 1 tahun. Bakteri Asam Laktat dapat di isolasi dari minuman Classic Enzyme. Tujuan Penelitian ini adalah untuk mengisolasi dan mendapatkan daya hambat antibakteri dari isolat BAL yang terdapat pada minuman classic enzyme. Penelitian ini merupakan jenis penelitian deskriptif kualitatif dan kuantitatif dengan uji organoleptik, pengukuran pH dan % kadar asam laktat. Isolat yang telah diisolasi dikarakterisasi secara makroskopis (warna, bentuk, ukuran koloni) dan mikroskopis (pewarnaan Gram dan pewarnaan endospora). Uji biokimia (uji katalase dan tipe fermentasi). Uji aktivitas antibakteri terhadap bakteri patogen dengan metode difusi cakram. Hasil Penelitian organoleptik classic enzyme memiliki aroma khas fermentasi, rasa asam, warna kuning muda keruh dan tekstur cair dengan pH 4,70 dan kadar asam laktat rata-rata 1,0717%. Dari isolasi 6 isolat yaitu BAL 1, BAL 2, BAL 3, BAL 4, BAL 5 dan BAL 6. Karakteristik makroskopis semua BAL berwarna putih susu, BAL 1, BAL 2, BAL 3 dan BAL 6 berbentuk batang sedangkan BAL 4 dan BAL 5 berbentuk bulat. Ukuran koloni berkisar 2,0-2,3 mm. pH sampel 4,70. Karakteristik mikroskopis semua isolat mempunyai pewarnaan gram positif dan endospora negatif. Karakteristik uji biokimia semua isolat katalase negatif dan tipe fermentasi homofermentatif. Semua isolat BAL dapat menghambat pertumbuhan bakteri Staphylococcus aureus dengan diameter zona hambat berkisar 8,8 mm hingga 10,6 mm. Dari hasil tersebut disimpulkan bahwa isolat yang diisolasi dari Classic enzyme adalah Bakteri Asam laktat yang memiliki aktivitas antibakteri yang berpotensi sebagai sumber probiotik.
Co-Authors Adelia Ramadani Adelia Ramadani Adli Nuzula Rahmah Alfi Wahyudi Nasution Amaliana, Amaliana Anny Sartika Daulay Anny Sartika Daulay Anny Sartika. D Ayu , Tri Indah Pagar Barutu, Liza Anisa Shevia Cindy Widya Rosa Br Simarmata D Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang Delvi Amira Hutagaol Dena, Mutia Diana Sopha Dikki Miswanda Elma Natasya Fatikasari, Cilki Fithri Pulungan, Ainil Fitri Mulyani, Fitri Gabena Indrayani Dalimunthe Gultom, Syarifah Elena Habibie, Dian Halim, Rahman Harahap, Azmi Kurniawan Harahap, Siti Salimah Haris Munandar Nasution Hendri Gunawan Hutagaol, Delvi Amira Huzeila Nisa Siregar Ika Fitriani Ika Yeni Siahaan Khofifah , Nur Lailatulqodri, Najwa Lastri Afni Leni Safriani Linda, Rosa Lisda Mayanti Lubis, Minda Sari Lubis, Nadya Frestika Lulu Ilma Khoirun Nissa M Pandapotan Nasution M Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution Merani Phaustina Lumban Gaol Muhammad Amin Nasution Muhammad Arif Muhammad Hizbullah Muhammad Pandapotan Nasution Muhammad Wahyu Ikhsan Muhammad Wahyu Ikhsan Munaziatul Jannah Nadia Salsabilla Nanda Nadia Nasution , Muhammad Amin Nasution, M. Pandapotan Nelsa Fahira Nofa Lismandaria Nomi Noviani Novi Yuliandari Novika Utami Novriani Putri Santia Nurul Hasanah Oca Putri Nazuhra Oktavia, Sri Rahayu Prasetyo , Healthy Aldriany Rafita Yuniari Rafita Yuniart Rafita Yuniarti Rafita Yuniarti Rati Satri Situmorang Ridwanto Ridwanto Rosalinda Mahdalena Sinaga Rosalinda Mahdalena Sinaga Rossa, Aliffa Safira , Liyuza Selfiani Selfiani Sepriani, Ananda Shilvia, Fatin Silitonga, Burju Artha Marshalina Sinaga, Rosalinda Mahdalena Siregar, Ahmad Gozali Siti Aisyah Jamil Sony , Suyefri Sri Wahyuni Supiyani, Supiyani Syahni, Cynthia Syarifah Elena Gultom Tawir Rina Rezeki Tusaddiah, Ria Winata, Ageng Tegar Yani, Farida Yayuk Yuliana Zulmai Rani