Claim Missing Document
Check
Articles

AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS EKSTRAK ETANOL DAUN KOPI LIBERIKA (COFFEA LIBERICA W. BULL EX HIERN) Linda, Rosa; Rahayu, Yayuk Putri; Dalimunthe, Gabena Indrayani; Nasution, Haris Munandar
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 1 (2025): APRIL 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i1.36810

Abstract

Daun kopi liberika (Coffea liberica W. Bull ex Hiern) mengandung senyawa flavonoid, alkaloid, saponin, polifenol dan lainya yang memiliki bioaktivitas sebagai antioksidan, antibiotik, dan antiinflamasi. Senyawa fenolik (flavonoid) berkorelasi dengan aktivitas antioksidan. Antioksidan dapat menstabilkan kekurangan elektron dari radikal bebas sehingga sel-sel terlindungi dari senyawa reaktif. Banyaknya radikal bebas didalam tubuh dapat mengakibatkan berbagai penyakit seperti kanker. Penelitian ini bertujuan untuk melihat kadar total fenolik, flavonoid, aktivitas antioksidan dan toksisitas daun kopi liberika. Penelitian dilakukan secara eksperimental. Data yang dikumpulkan merupakan data kuantitatif dan kualitatif yang mana diambil dari hasil pengumpulan sampel, karakteristik simplisia, skrining fitokimia, penetapan kadar fenolik dan flavonoid, uji aktivitas antioksidan menggunakan metode 1,1-Diphenyl-2-Picrylhidrazyl (DPPH), uji toksisitas menggunakan metode Brine Shrimp Lethality Test (BSLT) pada larva udang Artemia salina Leach. Metode BSLT merupakan metode uji pendahuluan toksisitas senyawa antikanker. Hasil penelitian ini menunjukkan bahwa ekstrak dan simplisia daun kopi liberika mengandung senyawa kimia yaitu alkaloid, fenolik, flavonoid, saponin, tanin, glikosida dan steroid. Simplisia daun kopi liberika memiliki karakteristik sesuai persyaratan mutu MMI Edisi 5. Hasil penetapan kadar total fenolik yaitu 227,21 mgGAE/g ekstrak dan flavonoid 85,66 mgQE/g ekstrak. Hasil uji aktivitas antioksidan diperoleh nilai IC50 = 48,56 µg/mL dengan kategori sangat kuat, dan hasil uji toksisitas diperoleh nilai LC50 = 143,208 µg/mL dengan kategori sangat toksik. Hal ini menunjukkan bahwa daun kopi liberika memiliki potensi sebagai antikanker karena memiliki antioksidan yang sangat kuat dan toksisitas yang sangat tosik.
Perbedaan Media Fermentasi Terhadap Produksi Protein Sel Tunggal (PST) Menggunakan Kultur Saccharomyces cerevisiae Rahayu, Yayuk Putri; Nasution , Haris Munandar; Prasetyo , Healthy Aldriany
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.846

Abstract

Utilization of pineapple peel waste and tofu liquid waste can reduce environmental impacts and is an alternative fermentation medium containing cheap and easily obtained carbohydrate sources and has the potential for the growth of Saccharomyces cerevisiae. Single Cell Protein (SCP) is a protein derived from microbes such as yeast. The purpose of this study was to determine the differences between pineapple peel waste and tofu liquid waste with the addition of different nutrients to SCP production using S. cervisiae. The research method was experimental. The independent variables were fermentation medium MK1 (pineapple peel waste; KH2PO4; sugar); MK2 (pineapple peel waste; KH2PO4; (NH4)2SO4; sugar), MT1 (tofu liquid waste; KH2PO4; sugar); and MT2 (tofu liquid waste; KH2PO4; (NH4)2SO4; sugar). The fermentation period was 0, 2, 4, and 6 days. The dependent variables were protein content, cell dry weight, glucose content, pH and temperature. The results of the SCP production research obtained the highest percentage increase in protein content in MT2 media (tofu liquid waste; KH2PO4; (NH4)2SO4; sugar) was 33.33% (day-2, increase in dry cell weight 84.90%), followed by MK2 media (pineapple peel waste; KH2PO4; (NH4)2SO4; sugar) was 13.15% (day-4, increase in dry cell weight 2.77%). The conclusion is that from both pineapple peel waste and tofu liquid waste substrates, the best PST production was obtained with the highest protein content in MT2 (tofu liquid waste media with the addition of KH2PO4; (NH4)2SO4; and sugar).
Formulasi dan Uji Aktivitas Antibakteri Sediaan Sabun Cair Ekstrak Etanol Daun Kopi Arabika (Coffea arabica L.) Terhadap Bakteri Staphylococcus aureus Amaliana, Amaliana; Nasution, Haris Munandar; Lubis, Minda Sari; Rahayu, Yayuk Putri
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.872

Abstract

Coffee leaves (Coffea arabica L.) are a part of the coffee plant that is not widely used. Coffee leaves contain flavonoids, alkaloids, saponins, caffeine and polyphenols. Coffee leaves are a natural ingredient that has antibacterial and antioxidant activity, so they need to be used. develop it into pharmaceutical preparations to increase ease of use, one of which is liquid soap preparations The aim of this research was to determine the secondary metabolite content of simplicia powder and ethanol extract of coffee leaves, formulate ethanol extract of coffee leaves into liquid soap, determine the physical quality of the preparation and test the activity against Staphylococcus aureus bacteria. The method used in this research is to make a liquid soap formulation from Arabica coffee leaves and test its effectiveness as an antibacterial against Staphylococcus aureus bacteria. This research was carried out experimentally with the independent variable being the concentration of Arabica coffee leaf ethanol extract: F1 contains 2.5 grams of active substance, F2 contains 5.0 grams of active substance, F3 contains 7.5 grams of active substance, dependent variable: leaf simplicia phytochemical screening test Arabica coffee, antibacterial activity test against Staphylococcus aureus bacteria. Quality of liquid soap preparations from ethanol extract of Arabica coffee leaves using stability tests (pH of the preparation, homogeneity, viscosity and height of foam) and antibacterial tests using the agar diffusion method (well method). The research results show that simplicia powder and ethanol extract of coffee leaves contain alkaloid, flavonoid, tannin and saponin chemical compounds. The organoleptic test results of liquid soap have a distinctive smell, a combination of coffee and rose leaves, are blackish brown in color, and are in the form of a thick liquid. The stability test results of all formulas are stable. The pH test ranges from 9.05 to 9.43. Test foam height 35- 40 mm. The viscosity test results ranged from 735.0-894.8 cpoise, antibacterial activity had an k- (Blanko) concentration inhibition zone of  13,6 mm FI concentration liquid soap obtained a result of 15.7 mm, FII concentration liquid soap obtained a result of 18.4 mm, FIII concentration liquid soap obtained a result of 18.75 mm compared to the control positive obtained a result of 18.8 mm.
Konsentrasi Hambat Minimum dan Konsentrasi Bunuh Minimum Ekstrak Dan Nanopartikel Ekstrak Daun Pometia pinnata Terhadap Staphyloccocus aureus dan Escherichia coli Sony , Suyefri; Rahayu, Yayuk Putri; Nasution , Haris Munandar; Rani, Zulmai
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.885

Abstract

Background: Infectious diseases caused by bacteria such as Escherichia coli and Staphylococcus aureus have become a global health issue. Matoa leaves (Pometia pinnata) are known to contain antibacterial compounds that hold potential as alternative treatments. Objective: This study aimed to produce nanoparticles from the ethanol extract of matoa leaves and compare the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values of both the extract and its nanoparticles against Escherichia coli and Staphylococcus aureus. Methods: This experimental research used different concentrations of matoa leaf ethanol extract (6.25%, 12.5%, 25%, 50%) and nanoparticle extract (0.625%, 1.25%, 2.5%, 5%). Nanoparticle characterization was performed using a Particle Size Analyzer (PSA). Results: The nanoparticle characterization showed a size of 528.95 nm. The MIC value of the matoa leaf extract against Escherichia coli and Staphylococcus aureus was 12.5%, while for the nanoparticles, it was 1.25%. The MBC value of the matoa leaf extract was 50%, and for the nanoparticles, it was 5%. The highest antibacterial activity of the ethanol extract was 18.3 mm and 18.4 mm, while for the nanoparticles, it was 18.5 mm and 19.0 mm. Conclusion: The nanoparticle extract of matoa leaves was more effective in inhibiting and killing bacteria compared to the regular extract, with a lower dose (1:10), showing its potential as an efficient antibacterial alternative.
Edukasi Masyarakat Dalam Pembuatan Probiotik Alami Dalam Meningkatkan Kesehatan Ternak di Desa Binjai Bakung Kecamatan Pantai Labu Kabupaten Deli Serdang Wahyuni, Sri; Rahayu, Yayuk Putri; Noviani, Nomi; Habibie, Dian; Yani, Farida
Jurnal Pengabdian West Science Vol 4 No 06 (2025): Jurnal Pengabdian West Science
Publisher : Westscience Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58812/jpws.v4i06.2339

Abstract

Penggunaan probiotik telah menjadi alternatif penting dalam praktik peternakan untuk mengatasi resistensi antibiotik dan meningkatkan kesehatan ternak. Program pengabdian masyarakat ini bertujuan meningkatkan pengetahuan dan keterampilan masyarakat, khususnya peternak di Desa Binjai Bakung, Kecamatan Pantai Labu, Kabupaten Deli Serdang, dalam memahami serta membuat probiotik alami untuk ternak. Metode pelaksanaan meliputi ceramah, diskusi, dan praktik langsung pembuatan probiotik. Hasil Pre Test menunjukkan pemahaman dan keterampilan awal peserta yang bervariasi, dengan hanya 50% memahami probiotik, 75% mengetahui contoh probiotik (umumnya untuk manusia), dan 30% mampu membuat probiotik alami. Setelah program, evaluasi melalui Post Test menunjukkan peningkatan luar biasa dengan tingkat keberhasilan 100% pada seluruh indikator: 20 dari 20 peserta memahami pengertian dan manfaat probiotik, 20 dari 20 peserta mampu menyebutkan contoh produk probiotik, dan 20 dari 20 peserta mampu membuat probiotik alami untuk ternak. Keberhasilan 100% ini membuktikan efektivitas metode dan materi yang disampaikan. Program ini tidak hanya meningkatkan kapasitas individu peserta tetapi juga berpotensi memberikan dampak positif pada pemberdayaan ekonomi lokal dan praktik peternakan yang berkelanjutan dengan mengurangi ketergantungan pada pakan komersial dan antibiotik. Untuk memastikan keberlanjutan dan dampak jangka panjang, direkomendasikan adanya pendampingan pasca-pelatihan, pembentukan kelompok peternak, evaluasi dampak komprehensif, serta menjalin kemitraan untuk pengembangan program lebih lanjut.
Pelatihan pengujian Formalin dan Boraks pada makanan dengan Reaksi warna alami di Persatuan Wirid Batak Islam (PWBI) Kelurahan Kwala Bekala Medan Supiyani, Supiyani; Rahayu, Yayuk Putri; Nasution, Haris Munandar; Hizbullah, Muhammad
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 5, No 3 (2025): Abdira, Juli
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v5i3.833

Abstract

In an effort to improve public health by reducing the use of hazardous food additives in the city of Medan, community service has been carried out at the Perwiritan Batak Islam (PWBI) in Kwala Bekala, Medan Johor, Medan city on December 19-20, 2024. The training focused on learning how to test for formalin and borax in food using the natural color reaction of turmeric and KMnO4 (blue medicine) with 18 participants. This activity contributed to enhancing the technical skills of testing for formalin and borax to prevent and reduce the risk of using hazardous food additives. After this training, participants' knowledge about natural color tests on food containing formalin and borax improved. Pre-test and post-test evaluations of 20 participants showed a significant improvement in their understanding, with evaluation graphs indicating a shift from a low level of knowledge to a better level of knowledge.
Uji Aktivitas Antibakteri Formulasi Sediaan Obat Kumur Ekstrak Etanol Daun Senduduk (Melastoma malabathricum L.) Terhadap Pertumbuhan Streptococcus mutans Ayu , Tri Indah Pagar; Nasution , Haris Munandar; Rahayu, Yayuk Putri; Nasution , Muhammad Amin
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 3 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i3.1002

Abstract

Background: Dental caries is a common oral health problem caused by Streptococcus mutans. Antiseptic mouthwash containing natural ingredients like senduduk leaves (Melastoma malabathricum L.) shows potential as an alternative, as it contains antimicrobial compounds (alkaloids, flavonoids, saponins, and tannins). Objective: To determine the antibacterial activity of senduduk leaf ethanol extract mouthwash against S. mutans and evaluate its physical characteristics. Methods: This experimental study included: (1) ethanol extraction of senduduk leaves using 96% ethanol maceration, (2) simplicia characterization and phytochemical screening, (3) formulation of three mouthwash concentrations (2.5%, 5%, 7.5%), (4) preparation evaluation (organoleptic properties, pH, viscosity), and (5) antibacterial activity testing using the well diffusion method. Results: The mouthwash preparation met physical criteria as a light-to-dark brown liquid with pH 6.33-6.43 and viscosity of 4.833-5.726 cps. Antibacterial tests showed dose-dependent effects, with the highest inhibition zone in the 7.5% formula (18.53 mm; strong category). Conclusion: Senduduk leaf extract shows potential as an antibacterial active ingredient in herbal mouthwash against S. mutans in vitro, though further testing (in vivo/clinical trials) is needed to validate therapeutic claims.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN TERONG HIJAU (SOLANUM MELONGENA) DALAM KEBOCORAN DNA DAN PROTEIN BAKTERI Barutu, Liza Anisa Shevia; Nasution, Haris Munandar; Rahayu, Yayuk Putri; Pulungan, Ainil Fithri
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 2 (2025): AGUSTUS 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i2.45009

Abstract

Penyakit menular merupakan masalah kesehatan yang sering disebabkan oleh berbagai spesies bakteri. Escherichia coli mewakili patogen umum. Enzim metabolisme yang terdapat pada daun terong hijau (Solanum melongena) memiliki sifat antibakteri. Tujuan dari penelitian ini adalah untuk mengevaluasi intensitas aktivitas bakteri dan kebocoran DNA dan protein bakteri. Penelitian ini bersifat eksperimental, dengan dilakukan uji karakterisasi simplisia dan daun terong hijau di ekstraksi dengan metode maserasi. Analisis kimia terhadap serbuk dan ekstrak dilakukan, kemudian diuji aktivitas antibakterinya dengan cara difusi pada kertas pada suhu 37oC selama 24 jam. Kebocoran DNA dan protein bakteri diverifikasi dengan spektrofotometri UV-visibel. Daun terong hijau mengandung alkaloid, flavonoid, glikosida, tanin, saponin dan steroid, menurut hasil percobaan. Berdasarkan konsentrasi 2,5, 5, 7,5 dan 10%, hasil uji aktivitas antibakteri daya hambat ekstrak daun terong hijau adalah 6,7, 7,7, 8,1 dan 9,9 mm. Konsentrasi DNA dan protein 10% pada kelompok perlakuan sedikit lebih tinggi dibandingkan pada kelompok kontrol positif, dengan nilai serapan pada 260 nm (DNA) dan 280 nm (protein).
Pengabdian Kepada Masyarakat melalui Kuliah Kerja Nyata (KKN) dalam Membangun Daerah yang Berkelanjutan di Kelurahan Indrasakti Kecamatan Air Putih Rahayu, Yayuk Putri; Fatikasari, Cilki; Oktavia, Sri Rahayu; Tusaddiah, Ria; Winata, Ageng Tegar; Harahap, Azmi Kurniawan
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 5, No 4 (2025): Abdira, Oktober
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v5i4.1060

Abstract

Community Service (PKM) through Real Work Lecture (KKN) was carried out in Indrasakti Village, Air Putih District, Batu Bara Regency, North Sumatra. This activity aims to increase the role of lecturers and students in providing socialization, education, and improving the entrepreneurial spirit, as well as developing community skills towards sustainable regional development. The methods used are socialization, education and skills training on making used cooking oil soap, making organic waste ecoenzymes, implementing clean and healthy living (PHBS), making MSME financial records, public health checks, and environmental cleanliness mutual cooperation. The stages of this activity are survey and collaboration with the village head; preparation; and counseling, socialization, and education in delivering materials. The results of this PKM KKN activity show an increase in community knowledge and skills with an average knowledge score of 42.5 increasing to 75.8. The conclusion of this activity was an increase in community knowledge and skills by 78.43% which had an impact on improving the quality of life and sustainable regional development in Indrasakti Village.
UJI AKTIVITAS ANTIBAKTERI NANOPARTIKEL EKSTRAK ETANOL DAUN MANGGA HARUM MANIS (Mangifera indica L. var. arum manis) TERHADAP BAKTERI Escherichia Hutagaol, Delvi Amira; Rahayu, Yayuk Putri; Nasution, M. Pandapotan; Nasution, Haris Munandar
Jurnal Farmasi Klinik dan Sains Vol 3, No 1 (2023): Jurnal Farmasi Klinik dan Sains
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Gombong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jfks.v3i1.1109

Abstract

Bakteri Escherichia coli merupakan salah satu bakteri penyebab penyakit diare. Daun mangga harum manis (Mangifera indica L. var. harum manis) diketahui memiliki kandungan senyawa antibakteri. Dalam ukuran nanopartikel, luas kontak permukaan partikel menjadi lebih besar sehingga dapat meningkatkan aktivitas antibakteri. Tujuan penelitian ini untuk membandingkan aktivitas nanopartikel dengan ekstrak etanol daun mangga harum manis terhadap bakteri E. coli. Penelitian ini merupakan penelitian eksperimental dengan variabel bebas yaitu konsentrasi ekstrak etanol daun harum manis (EEDM 25%; EEDM 50%; dan EEDM 75%) dan konsentrasi nanopartikel ekstrak etanol daun mangga harum manis (NEDM 2,5%; NEDM 5%; dan NEDM 7,5%) dengan antibiotic pembanding yaitu tetracyclin.Variabel terikat yaitu aktivitas antibakteri ekstrak etanol dan nanopartikel ekstrak daun mangga harum manis terhadap bakteri E. coli. Karakterisasi nanopartikel ekstrak etanol menggunakan Particle Size Analyzer (PSA). Pengujian aktivitas antibakteri menggunakan metode difusi agar cakram Kirby Bauer. Hasil karakteristik ukuran nanopartikel ekstrak adalah 40,2 nm. Nilai Zone of Inhibition (ZOI) ekstrak etanol daun mangga harum manis adalah 12,4 mm (EEDM 25%); 14,1 mm (EEDM 50%); dan 16,1 mm (EEDM 75%). Nilai ZOI nanopartikel ekstrak etanol daun mangga harum manis adalah 13,8 mm (NEDM 2,5%); 14,5 mm (NEDM 5%); dan 18,6 mm (NEDM 7,5%). Kesimpulannya adalah ekstrak etanol daun mangga harum manis dapat dijadikan nanopartikel ekstrak dengan daya aktivitas antibakteri konsentrasi nanopartikel ekstrak 5% setara efektivitasnya dengan konsentrasi ekstrak etanol daun mangga harum manis 50% terhadap E. coli dengan kategori intermediate dibandingkan dengan Tetracyclin 30 μg, sehingga dapat dikatakan bahwa nanopartikel esktrak dapat memperkecil dosis suatu obat.
Co-Authors Adelia Ramadani Adelia Ramadani Adli Nuzula Rahmah Alfi Wahyudi Nasution Amaliana, Amaliana Anny Sartika Daulay Anny Sartika Daulay Anny Sartika. D Ayu , Tri Indah Pagar Barutu, Liza Anisa Shevia Cindy Widya Rosa Br Simarmata D Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang Delvi Amira Hutagaol Dena, Mutia Dikki Miswanda Elma Natasya Fatikasari, Cilki Fithri Pulungan, Ainil Fitri Mulyani, Fitri Gabena Indrayani Dalimunthe Gultom, Syarifah Elena Habibie, Dian Halim, Rahman Harahap, Azmi Kurniawan Harahap, Siti Salimah Haris Munandar Nasution Hendri Gunawan Hutagaol, Delvi Amira Huzeila Nisa Siregar Ika Fitriani Ika Yeni Siahaan Khofifah , Nur Lailatulqodri, Najwa Lastri Afni Leni Safriani Linda, Rosa Lisda Mayanti Lubis, Minda Sari Lubis, Nadya Frestika Lulu Ilma Khoirun Nissa M Pandapotan Nasution M Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution Merani Phaustina Lumban Gaol Muhammad Amin Nasution Muhammad Arif Muhammad Hizbullah Muhammad Pandapotan Nasution Munaziatul Jannah Nadia Salsabilla Nanda Nadia Nasution , Muhammad Amin Nasution, M. Pandapotan Nelsa Fahira Nofa Lismandaria Nomi Noviani Novi Yuliandari Novika Utami Novriani Putri Santia Nurul Hasanah Oca Putri Nazuhra Oktavia, Sri Rahayu Prasetyo , Healthy Aldriany Rafita Yuniari Rafita Yuniart Ridwanto Ridwanto Rosalinda Mahdalena Sinaga Rosalinda Mahdalena Sinaga Rossa, Aliffa Safira , Liyuza Selfiani Selfiani Sepriani, Ananda Shilvia, Fatin Silitonga, Burju Artha Marshalina Sinaga, Rosalinda Mahdalena Siregar, Ahmad Gozali Siti Aisyah Jamil Sony , Suyefri Sopha, Diana Sri Wahyuni Supiyani, Supiyani Syahni, Cynthia Syarifah Elena Gultom Tawir Rina Rezeki Tusaddiah, Ria Winata, Ageng Tegar Yani, Farida Yayuk Yuliana Yuniarti, Rafita Zulmai Rani