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All Journal Jurnal Ilmu dan Kesehatan Hewan Jurnal Veteriner Indonesia Medicus Veterinus Media Kedokteran Hewan Journal of Health (JoH) GHIDZA: Jurnal Gizi dan Kesehatan Jurnal Medik Veteriner Community Development Journal: Jurnal Pengabdian Masyarakat Nutriology: Jurnal Pangan, Gizi, Kesehatan Muhammadiyah Journal of Nutrition and Food Science (MJNF) Journal of Innovation Research and Knowledge International Journal of Agriculture System Innovative: Journal Of Social Science Research Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences Kupang Journal of Food and Nutrition Research Ulil Albab Joong-Ki

Anak Agung Ayu Mirah Adi

Laboratorium Patologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234

Author-ID : 554997

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Gambaran Histopatologi Selaput Lendir Kantung Empedu Sapi Bali yang Terinfeksi Cacing Fasciola gigantica (HISTOPATOLOGICAL ILLUSTRATION OF GALL BLADDER MUCOUS MEMBRANE INFASCIOLA GIGANTICA INFECTED BALI CATTLE) I Made Kardena; Ida Bagus Oka Winaya; Elyda .; I Dewa Made Adhiwitana; AAA Mirah Adi; I Ketut Berata
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study aims were to describe lesions and their intensities of histopathological changes in gallbladder mucosa of bali cattle that infected with Fasciola gigantica. As many as 42 gall bladers of balicattle were used in this studi, i.e: 35 samples were infected and sefen were non-infected with F.gigantica.The samples were randomly gathered from Pesanggaran slaughter house in Denpasar. The gall bladderswere evaluated macroscopically and prepared for microscopically examination using haematoxylin andeosin staining.The result showed that there were histopathological changes observed in the mucosa of thegall bladder:necrosis cells of gall bladder with intencity 100%, hemorrhage 68.5%, hyperplasia serousgland and mucous hypertrophy gland 100%, collagen in filtration 100%, fibroblast proliferation 91.4%,and infiltration of inflammation cells, 74.2%. Histopathological changes in gallbladders mucous of balicattle infected F.gigantica experiencing severe lesons with frequency percentage above 50%.

Gambaran Histopatologi Kulit Anjing Penderita Dermatitis (HISTOPATHOLOGICAL FEATURES OF DOG’S SKIN WITH DERMATITIS) Komang Andika Purnama; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Gusti Made Krisna Erawan; I Made Kardena; I Nyoman Suartha
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Complex dermatitis is a disorder of the skin caused by the combination of several agents such as fungi, ectoparasites, bacteria, and metabolic diseases with combined clinical symptoms of primary lesions and secondary lesions. This study aims to determine the histopathology of dog skin with complex dermatitis. There were 15 samples of dogs with complex dermatitis, skin biopsy and histopathology. The results showed that the incidence of complex dermatitis in young dogs was 60%, long-haired 67%, male sex 73%, and domestic race 73%. Macroscopic examination found a change in hyperkeratosis. Microscopically observed that infiltration of polymorphonuclear cells, monomorphonuclear cells and macrofag from moderate to severe in the dermis. In hair follicles and epidermis occured akantosis, and keratin proliferation. There were segment fragments from infectious agents such as Demodex canis, Sarcoptes scabiei, and yeast from Malasezia sp. Other changes were observed in the presence of hydrophic degeneration, necrosis, and ulcers. Dogs suffering from complex dermatitis have chronic infections with more than one infectious agent. Disabilities based on histopathological observation of dogs with complex dermatitis with moderate severity of 33%, and severe 67%.

Studi Kasus: Ascariosis Disertai Migrasi Larva pada Hati dan Paru-paru Babi Landrace (CASE STUDY: ASCARIOSIS ACCOMPANIED BY MIGRATION OF LARVAE IN LIVER AND LUNGS OF LANDRACE PIG) I Ketur Berata; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Made Kardena; Ida Bagus Windia Adnyana
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Cases of ascariosis due to infection with Ascaris suum are still prevalent in the world, including Indonesia. Apart from causing economic losses, ascariosis can also threaten human health because it is zoonotic. Case studies of ascariosis with typical white spot lesions in the landrace pig liver is described in this paper. Pigs aged 60 days old, sick for 10 days, thin and coughing gathered from the village of Suwung, Denpasar City. After necropsy and post mortem examination were done, tens of A. suum worms were found in the intestine and white spot lesions observed on the livers. Tissues of small intestine, large intestine, liver, lungs, kidney, spleen, urinary vesicles and brain were taken and put into 10% of formalin neutral buffer before they then processed in making histopathological preparations. Histopathological preparation was carried out using paraffin block method and hematoxylin eosin (HE) staining technique. The results of histopathological examination of the small intestine, large intestine, liver and lungs were found to be infiltrated by eosinophil cells. Eosinophil cells infiltration indicates that there are parasites A. suum or the larvae migrant in the tissues. Some of the tissues in livers were also found to have fibrosis, which is suspected that the infection has been chronic. It can be concluded that cases of the landrace pigs were chronically infected by A.suum and accompanied by migration of the larvae to the livers and lungs. More attention is needed to prevent the increase of ascariosis in pigs in order to minimize economic losses and transmission to humans.

Pelacakan Secara Imunohistokimiawi Antigen Virus pada Ayam yang Diinfeksi dengan Virus Penyakit Tetelo (IMMUNOHISTOCHEMICAL DETECTION OF VIRAL ANTIGEN IN TISSUE OF CHICKENS EXPERIMENTALLY INFECTED WITH NEWCASTLE DISEASE VIRUS) Anak Agung Ayu Mirah Adi; I Made Kardena; Nyoman Mantik Astawa; Yasunobu Matsumoto
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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In order to study the distribution of Newcastle disease virus (NDV) following infection, chickenswere experimentally infected with visceretropic velogenic NDV isolate. Monoclonal antibodies (mAbs)against the NDV LaSota vaccine strain were then produced to detect viral antigen in the infectedorgans. The mAbs were firstly tested for their specificity by enzyme linked immunosorbent assay(ELISA) using NDV and normal allantoic fluids as antigens. Eight mAbs specific against NDVwere isolated and two mAbs were used for immunodetection of NDV antigen in chicken’s tissues.By immunohistochemistry labeled streptavidin-biotin (LSAB) staining NDV–antigen was detectedin paraffin embedded tissues of NDV-infected chickens. NDV antigen was not detected in noninfected chickens. In the infected chickens, high intensity of NDV antigen was detected in thelymphoid tissues, lung and intestine. The NDV antigen with a lesser intensity was detected in thebrain, trachea, liver and myocardium. This study shows that although viscerotropic velogenicNDV isolate can infect almost all organs, the main target of infection are lung, intestine andlymphoids tissues

Mosquito-specific viruses (family Flaviviridae, genus Flavivirus) Diisolasi pada Nyamuk Anopheles vagus di Bali Putu Ayu Asri Damayanti; I Nyoman Mantik Astawa; Anak Agung Ayu Mirah Adi; I Made Sudarmaja; I Kadek Swastika; Dewa Ayu Agus Sri Laksemi; Ni Luh Putu Eka Diarthini
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Mosquito-specific viruses (MSVs) adalah virus yang hanya dapat bereplikasi pada sel nyamuk. Virus ini terdiri dari berbagai genus, salah satunya yang paling banyak ditemukan adalah dari famili Flaviviridae, genus Flavivirus. Namun, data keberadaan dan karakteristik MSVs dan vektornya di Bali saat ini sangat terbatas. Oleh karena itu, pengamatan untuk memperluas penemuan keragaman vektor dan filogenetik MSVs famili Flaviviridae, genus Flavivirus di Bali dilakukan pada tahun 2016-2018. Nyamuk dewasa ditangkap menggunakan light trap dan dikelompokkan berdasarkan spesies. Isolasi dan propagasi virus dilakukan pada galur sel C6/36 dan baby hamster kidney-21 (BHK-21). Identifikasi virus dilakukan dengan menggunakan one step reverse-transcriptase polymerase chain reaction (RT-PCR). Terdapat dua pool yang berasal dari nyamuk Anopheles vagus menampakan cythopathic effect (CPE) hanya pada galur sel C6/36 dari total 158 pool. Virus yang diisolasi memiliki persentase identity sekuen nukleotida tertinggi 97% dan sekuen asam amino 96% dengan virus Culex theileri Flavivirus isolat JKT-8650 yang diisolasi pada tahun 1981. Selanjutnya, virus dinamakan Mosquito Flavivirus Isolate Bali (MFB) dengan accession numbers KY995166 dan KY290258. Analisis filogenetik menunjukan bahwa MFB berada satu kluster dengan Culex theileri Flavivirus (CTFV) dari Indonesia, Culex Flavivuruses-Myanmar, Culex theileri Flavivirus-Portugal, dan Mosquito Flavivirus-Turki. Terdapat delapan nukelotida dan enam asam amino yang berbeda antara MFB dan CTFV Indonesia. Pada penelitian ini dapat disimpulkan bahwa MSVs dari famili Flaviviridae, genus Flavivirus berhasil diisolasi dari nyamuk An. vagus di Bali.

Gambaran Histopatologi Hati dan Ginjal Anjing Terduga Leptospirosis di Kota Denpasar (HISTOPATHOLOGICAL CHANGES OF LIVER AND KIDNEY DOG SUSPECTED CANINE LEPTOSPIROSIS IN DENPASAR CITY) Ida Bagus Oka Winaya; I Ketut Berata; I Made Kardena; Anak Agung Ayu Mirah Adi; Aida Louise Tenden Rompis
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aims to determine the pathological changes in dog suspected of being infected by Leptospira spp. This research used a retrospective study design. A total of 210 canines sample were examined at Faculty of Veterinary Medicine The Udayana of University between January 2008 to January 2010. Of the 210 specimens, five canines with clinical sign anorexia, fever, vomiting, polyurea, dyspnea and only one are noted icteric in the sclera. Macroscopically : the anemic to petechial haemorrhagis was find in pulmo, icterus in liver with gall blader distention, kidney swollen with black color in hillus, spleen rather swollen with black color on end and mild hemorrhagis on intentines. The infected organ is inserted into a pot filled with neutral buffer formalin 10%. Then stained with haematoxyllin-eosin for the microscopic preparation. Microscopic examimination revealed the presence of spiral bacterial cells on the necrotic liver and kidneys tissue. Infiltration neutrophils and macrophages was also find in pulmonary and spleen tissue. Besided that intertubuler edematous were also observed under microscopic examination. It can be concluded changes associated with Leptospira spp infection can be found in liver and kidney tissue with changes such as necrosis, proliferation of kuffer cells and intertubular edematous.

Vaksin Gumboro Menyebabkan Imunosupresif pada Respons Primer Vaksin Penyakit Tetelo Ayam Pedaging Gusti Ayu Yuniati Kencana; Anak Agung Ayu Mirah Adi; Ida Bagus Komang Ardana; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The variety of Gumboro live vaccine strains (hot, intermediate, and mild) are available inIndonesia. The immunosuppresive effect of these vaccines under field conditions is not known.This research was conducted to determine this devastating effect of such vaccine strains on theimmune response of chickens vaccinated againts Newcastle disease (ND). Sixty chickens werekept separately in five groups (i.e. V1, V2, V3, V4, and K). At the age of seven days, group V1, V2,and V3 were given hot, intermediate, and mild strains of Gumboro live vaccine respectively whilethe other two groups recieved no Gumboro vaccine (V4 and K). At the age of 14 days, all groups,except group K which were kept as a negative control, were vaccinated against ND. The level ofantibody produced in response to ND vaccination was measured in sera collected at day 0, 7, 14,and 21 post ND vaccination using a standard micro-haemaglutination inhibition test. Data of theantibody titers were analyzed using analysis of variance followed by Duncan’s multiple range test.The results showed that all Gumboro vaccine strains still retain its immunosuppressive nature onhumoral immune response in chickens that later vaccinated against ND. The geometric meantiter (GMT) of anti-NDV antibody of group V4 (unvaccinated againts Gumboro) was significantlyhigher than that of group V1, V2, and V3, i.e. groups of chickens that had been given varietystrains of Gumboro vaccines, at the first and second week after ND vaccination (p<0.05). Thedifference of this immunosuppressivenes among variety of Gumboro vaccine strains need furtherclarification.

Perubahan Patologi Sistem Hepatobiliari Sapi Bali Terinfeksi Fasciola gigantica Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; I Made Kardena; Ida Bagus Windia Adnyana; Ida Bagus Kade Suardana
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Liver fluke infections is an important parasitic disease that common attack cattle, buffalo and others ruminant. The disease in the tropics is caused by Fasciola gigantica trematodes. Fasciolosis found in Bali cattle besides causing high economic loss also has the opportunity infect humans. This study aims to determine the pathology changes of Bali cattle hepatobiliary system infected by F. gigantica. In January2019 were examined a hundred of liver tissue Bali cattle. Based on the sex as many as 75 bali cattle are cows and 25 are bulls. On macroscopic examination was found fifteen bali cattles in gallbladder containing of F. gigantica. In positive Fasciolosis the surface of liver look uneven with enlargement of lymph nodes. The tissue of Bali cattle liver infected with F. gigantica is cut into 1x1x1 cm and put in a pot that has been filled with 10% neutral buffered formaline. The fixed tissue then processed in a tissue processor and stained with hematoxylin-eosin (HE). Microscopic examination was found bile ducts proliferation and blocked of the lumen accompanied by cholangio cirrhosis. Found longitudinal pieces of Fasciola giganticaliver fluke surrounded by fibrous connective tissue and multifocal coagulative necrosis with fibroblast proliferation. In some location the infiltration of neutrophil cells around the bile ducts can also be seen. Congestion, bleeding, and neutrophil infiltration are also seen in areas of necrosis. In the bile duct wall bleeding, adenomatus hyperplasia, infiltration of mononuclear cells with mild intensity and foci ofcalcification are found. It can be concluded that there was a change with severe intensity accompanied by chronic inflammation in the hepatobiliary system of Bali cattle with Fasciolosis.

Infeksi Avian Paramyxovirus Tipe-1 pada Babi di Bali (AVIAN PARAMYXOVIRUS TYPE-1 INFECTION IN PIG IN BALI) Anak Agung Ayu Mirah Adi; I Gusti Agung Arta Putra; Bayu Setiabudi; I Made Kardena; Nyoman Mantik Astawa
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Avian Paramyxovirus type 1 (APMV-1) is a virus infecting birds with mortality level varies accordingto its pathotype. Although the virus is normally originated from birds, the recent report showed that it canbe isolated from pig. In order to find out APMV-1 infections on pig in Bali, a serologic study was conductedto detect the antibody against APMV-1 among pigs in Bali. Indirect enzyme-linked immunosorbentassay (ELISA) was used to detect the antibody against APMV-1 in pig sera. The serum samples withoptical density value over the cut off value (COV) was considered as those containing Ab against APMV-1. Of 215 samples collected from traditional farms of eight regency and city in Bali namely Negara,Tabanan, Badung, Denpasar City, Gianyar, Bangli, Karangasem and Buleleng Regency, 174 samples(80,93%) were positive antibody against APMV-1. On the other hand, there were 125 (98,42%) positivesample among 127 sample collected from Pesanggaran abattoir. Furthermore, the 8-9 month age pigsshowed that their antibody against APMV-1 were higher (p<0.01) than those of 3-6 months age. TheELISA positive samples were further confirmed using Western immunoblotting assay. The anti-APMV-1antibody positive sera reacted with the virus specific protein band of 258 kDa and the negative sera did notreact with the viral protein. As pig has never been vaccinated with APMV-1 vaccine, the presence of Abagainst APMV-1 showed that the pigs were naturally infected with APMV-1.

Melacak Antigen Virus Gumboro pada Bursa Fabrisius dan Limpa Menggunakan Metode Elisa (DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS ANTIGEN IN BURSA OF FABRICIUS AND SPLEEN USING ELISA) Anak Agung Ayu Mirah Adi; Hernomoadi Huminto; Lies Parede Hernomoadi
Jurnal Veteriner Vol 2 No 4 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Co-Authors Agung Arta Putra, I Gusti AGUSTINA SETIA Aida Lousie Tenden Rompis Alberto Agustinho Pereira Da Costa Joao Anak Agung Bagus Bramardipa Anak Agung Bagus Bramardipa Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Aras, Fatmawati Aristawati, I Dewa Agung Ayu Irma Astuti Nur Astuti Nur ASWEROS UMBU ZOGARA Bayu Setiabudi Benu, Lolang Loriana Bire, Ienoliski Rohi BORO, REGINA MARIA Chiharu Hongo Costa, Santa Luciana LDV da da Costa, Santa Luciana Diaz Vera Dewa Ayu Agus Sri Laksmi Dzikri Nurma'rifah Takariyanti Eisaku Tamura Elyda . Fedri Rell Ferbian Milas Siswanto Gusti Ayu Yuniati Kencana Hernomoadi Huminto Hidayatullah, Lalu Syarif I Dewa Made Adhiwitana I Gede Hendra Prasetya Wicaksana I Gusti Agung Arta Putra I GUSTI AGUNG AYU AMBARAWATI I Gusti Agung Dewi Sarihati I Gusti Agung Gede Putra Pemayun I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Kadek Swastika I Ketur Berata I Ketut Berata I Ketut Berata, I I Ketut Eli Supartika I KETUT ELI SUPARTIKA I Ketut Suastika I Made Anom Sutrisna Wijaya I Made Galih Diparayoga I Made Kardena I Made Kardena, I I Made Merdana I Made Sudarmaja I Made Sukada i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Wayan Wirata I Wayan Wita Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Oka Winaya Ida Bagus Oka Winaya, Ida Ida Bagus Windia Adnyana Jane Austen Peni Jayawardhita, Anak Agung Gde Juni Gressilda L. Sine Kadek Karang Agustina Komang Andika Purnama Komang, Nadia Eprillia Sary Darma Ni L.Sin, Juni Gressilda Lies Parede Hernomoadi Loaloka, Meirina S Loaloka, Meirina S Loaloka Luh Made Sudimartini M. Farhan Al Ma'arif Mada, Maria Magdalena Ki Maria F Vinsensia D P Kewa Niron Maria Goreti Pantaleon Marissa Divia Dayanti Marissa Divia Dayanti Marson, Fransiska Gratia Sonita Meirina S Loaloka Meirina Sulastri Loaloka Mia Monica Nadira, Laras Ayu Ni Luh Putu Eka Diarthini Nita, Maria Helena Dua Nofantri, Lidia Nopita Wati, Ni Made Nur Baiti Palagan Senopati Sewoyo Palagan Senopati Sewoyo Pinem, Nuh Lasjuardi Purnasari, Maria Elisabeth Putra, I Putu Werdikta Jayantika Putu Agus Trisna Kusuma Antara Putu Ayu Asri Damayanti Putu Henrywaesa Sudipa Rahmat Grahadi Regina Maria Boro Ruwa idah, Ruwa Sampelimbong, Marissa Santa Luciana da Costa Sartika, Nola Alfien Sartika, Nola Alfieni Sembiring, Anita Ch Sewoyo, Palagan Senopati Sine, Juni Gressilda L Sine, Juni Gressilda L. Tampubolon, Yandri Putra Lumatas Wijaya, Anak Agung Gede Oka Wira, Amar Yasunobu Matsumoto Yeni Ratna Sari

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