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Variasi Genetik Gen Penyandi Protein Fusi dari Avian Paramyxovirus Tipe I di Bali (GENETIC VARIATION OF GENE ENCODING FUSION PROTEIN OF AVIAN PARAMYXOVIRUS TYPE-I IN BALI I Gusti Agung Arta Putra; Anak Agung Ayu Mirah Adi; Nyoman Mantik Astawa
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.56 KB)

Abstract

This study aims to determine the genetic variation of gene encoding fusion (F)-protein of avianparamyxovirus (APMV) type-1 isolated from chickens suffering of Newcastle disease (ND) found in achicken farm in the province of Bali throughout the year 2014. There are five isolates got from sick chickenscases/death suspected of being infected by APMV -1, but negative for avian influenza virus (AIV) infectioni.e: D5/AK/2014, B1/AK/2014, T1/ARP/2014, G1/AK/2014, and K1/ARP/2014. Sequence analysis of thefive isolates showed that three isolates of D5, B1 and G1 have a genetic distance of 11,1%, 10,2% and 8,2%when compared to the nucleotide sequence of Bali-1/07 isolated previously from Karangasem regency in2007. Meanwhile, the genetic distance of T1 and K1 isolates are 20,1% and 18% respectively as compareto the sequence of the Bali-1/07 isolate. The composition of amino acids at the cleavage sites enzyme of Fproteinfor 3 isolates of D5, B1 and G1 are R-R-Q- K-R-F, which is the typical characteristic of virulentvirus strain. Meanwhile, two isolates i.e T1 and K1 got from layer chicken had the amino acid sequence ofG- R-Q-G -R-L, which is group into avirulent virus strains. It was concluded that APMV-1 isolates foundin ND cases in the year of 2014 were caused by virulent isolate and were grouped into VII-genotype.Furthermore, It was found that there were two avirulent isolates
Establishment and Characterization of Benzo(a)pyrene-Induced Skin Tumor in Rats Palagan Senopati Sewoyo; Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Marissa Divia Dayanti; Anak Agung Bagus Bramardipa; I Ketut Eli Supartika
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.46 KB) | DOI: 10.19087/jveteriner.2022.23.1.1

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Quite a number of research on cancer therapy strongly require an animal model of cancer. One of the chemicals commonly used to induce cancer in animal models is benzo(a)pyrene due to its carcinogenic effects. This study aims were to describe the gross pathology of the tumor-induced by benzo(a)pyrene in an olive oil solution (w/v), identify the type of tumor histopathologically, and finally, determine the correlation between the duration of the rats experiencing tumor and it’s grade score. Tumor grade score is important to assess in order to determine tumor malignancy. This study consisted of 10 white rats (Rattus norvegicus) were given two treatments: a negative control treatment (K-) was injected with 0.1 mL of olive oil and a positive control treatment (K+) was injected with 0.1 mL of 0.3 % (w/v) benzo(a)pyrene in olive oil solution. Each treatment rats was kept in a cage and monitored regularly. When the tumors macroscopically appeared in the interscapular area and were observed until reached 4 cm in size, the rats were then sacrificed and necropsied. Tumors were observed for the gross pathology to examine the shape and color of them, then routinely processed for histopathological evaluation. The results showed that the tumors’ cells appeared to be round (1/5), irregular (2/5), and multilobular (2/5). Based on histopathological observation, the types of tumors observed were classical fibrosarcoma (2/5) and pleomorphic fibrosarcoma (3/5). There is a significant association between the duration of the rats experiencing tumors and the tumor grade. The longer the rats have tumors, the tumors tend to be more aggressive.
Pengaruh Hypnosis Lima Jari Terhadap Burnout pada Perawat di Ruang Isolasi Covid-19 Anak Agung Ayu Mirahadi; Ni Made Nopita Wati; Ika Setya Purwanti
Journal of Health (JoH) Vol 9 No 2 (2022): Journal of Health (JoH) - July
Publisher : LPPM STIKES Guna Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30590/joh.v9n2.446

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The Covid-19 pandemic is the biggest challenge to the health care system in the word today. Nurses as one of the medical personnel who are fighting at the forefront in fighting the Covid-19, face various problems such as an increase in workload and a psychological burden when caring for patients during the Covid-19. The high workload and the heavy psychological burden borne by nurses in caring for patients during the Covid-19, make nurses vulnerable to burnout. Burnout experienced by nurses when caring for patients caring for pateints during the Covid-19 pandemic. Burnout experienced by nurses when caring for patients during the Covid-19 pandemic is called pandemic burnout. Non-pharmacological therapy is expected to reduce burnout in nurses. Five-finger hypnosis is one of the most efeective non-pharmacological therapies to reduce one’s stress and anxiety. This study aims to determine the effect of five-finger hypnosis on burnout in nurses in the Covid-19 isolation room. This research uses pre-experimental research, with a one group pre-post test design. Sample in this study amounted to 22 people who werw selected through purposive sampling technique. Data was collected using a burnout pandemic questionnaire. The results of the study were analyzed using the Wilcoxon Sign Rank Test. The results showed that before the intervention was given, most of the respondents experienced high burnout as many as 11 people (50%) shile after being given the intervention there was a decrease where most of the respondents experienced moderate burnout as many as 13 people (59,1%) with p value 0,000.
Rice Productivity Growth During Nine Years in Badung Regency, Bali Province Anak Agung Keswari Krisnandika; I Made Anom Sutrisna Wijaya; I Gusti Agung Ayu Ambarawati; Anak Agung Ayu Mirah Adi; Eisaku Tamura; Chiharu Hongo
International Journal of Agriculture System VOLUME 7 ISSUE 2, DECEMBER 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.103 KB) | DOI: 10.20956/ijas.v7i2.1808

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The trend of rice productivity and its stability in Badung Regency are presented in this study. The area is a tourism-based economy without leaving the role of rice production to feed the people.  Time series data were collected from five sub districts covering 53 villages during 2008-2016 due to the completeness of the data. Variability of the data was observed from the coefficient of variance (C.V.) to decide rice productivity stability. This study also observed correlation between rainfall and rice productivity in the area. Result of the study shows that rice productivity trend in Badung Regency tend to decline during nine years of observation especially in 2014-2016. Three sub-districts had stable condition, while two sub-districts in tourism area contributed to the decline of this matter. Analysis using bi-plot revealed that there is no significant correlation between rainfall in sub-district and rice productivity, implying that water is available throughout the year.  In terms of stability, majority of villages (69.81%) had stable condition of rice productivity ranging from middle and high category. Other 30.19% villages were categorized as unstable ranging from low to high productivity. This condition showed that Badung Regency were able to maintain stability of rice productivity during nine years of observation. Implication of this study is to pay more attention to two sub districts in tourism area to improve their rice productivity such as implementation of good agricultural practices.
PHYLOGENETIC ANALYSIS OF NP AND HN GENE OF NEWLY ISOLATED NDV/Badung-02/AK/14 Ferbian Milas Siswanto; Anak Agung Ayu Mirah Adi
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.38 KB) | DOI: 10.21157/j.ked.hewan.v11i2.2492

Abstract

virus was isolated from suspected Newcastle disease (ND) chicken in backyard farm at Sibang Village, Badung Regency, Bali Province. The isolates were then propagated and confirmed for NDV serologically. RNA isolation was performed by standard Trizol method. Phylogenetic tree analysis of NP gene (nt1020-1561) and HN gene (nt7019-7754) were performed using sequence of Badung-02/AK/14 and selected NDV strains from GenBank. Based on the NP gene sequence, the newly isolate closely related with other NDV strains belong to genotype VII that previously isolated such as Banjarmasin-010/10, Bali-1/07, and Cockatoo/90 with genetic distance 0.2%, 12.6%, and 18.0% respectively. The genetic distance with LaSota/46 virus (genotype II) is 19.6%. Based on HN gene sequence, genetic distance of the Badung-02/AK/14 with other viruses belong to genotype VII such as Banjarmasin-010/10, Bali-1/07, and Cockatoo/90 are 0.4%, 3.7%, and 4.2% respectively. The genetic distance with LaSota/46 virus (genotype II) is 9.0%. There were no difference between the result of nucleotide and amino acid sequence analysis, both in NP and HN gene (P0.05).
ASPEK PATOLOGIS INFEKSI PARVOVIRUS PADA ANAK ANJING DI KOTA DENPASAR Ida Bagus Oka Winaya; I Ketut Berata; AAA Mirah Adi; I Made Kardena
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.083 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2619

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Telah dilakukan penelitian mengenai aspek patologis infeksi parvovirus pada anak anjing di Denpasar. Sebanyak 80 ekor anak anjing telah diperiksa pada Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Udayana periode tahun 2011- 2012. Enam belas ekor diantaranya menunjukkan gejala klinis berak darah, depresi, anoreksia, dan dehidrasi. Sinyalemen mengenai umur, jenis kelamin, dan ras juga dicatat. Perubahan patologi anatomi secara signifikan berupa enteritis haemorrhagis et necrotican dapat ditemukan pada semua anak anjing penderita. Kongesti dan nekrosis ditemukan pada epikardium. Gambaran yang bersifat anemia ditemukan pada jaringan limpa, ginjal, hati, sedangkan paruparu mengalami hiperemia. Secara mikroskopis, pada usus halus mengalami hiperemia disertai dengan infiltrasi limfosit, villi terlihat atropi dan nekrosis pada kripta Lieberkuhn. Nekrosis limfosit (limfositolisis) ditemukan pada folikel limpa. Kongesti dan nekrosis pada otot jantung namun intranuclear inclusion bodies hanya ditemukan pada satu anak anjing penderita. Penebalan ditemukan pada septa alveoli sedangkan pada hati dan ginjal hanya ditemukan peradangan ringan. Secara klinis bentuk enteritis hemorhagis et necrotican selalu ditemukan pada anak anjing terinfeksi parvovirus di Kota Denpasar. ________________________
KONFIRMASI AVIAN PARAMYXOVIRUS TIPE 1 (APMV-1) SECARA HISTOPATOLOGIS, SEROLOGIS, DAN MOLEKULER (Confirmation of Avian Paramyxovirus Tipe 1 (APMV-1) Infection by Histopathology, Serology, and Molecular Method) I Gusti Agung Arta Putra; Anak Agung Ayu Mirah Adi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.746 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5089

Abstract

Research was conducted to detect APMV-1 infection by examining microscopic lesions of chicken suspected ND and confirming the causative agent with serological and molecular assay. Samples obtained from commercial and back yard farm in 9 regencies and city of Bali Province were tested by rapid test for AIV antigen detection. AI negative samples were necropsied, then brain, lungs, and intestines were collected for histopathological examination. Samples tissue of brain, lung, spleen, and intestine were taken aseptically for viral isolation and amplification. Infected allantoic fluid was collected and tested by hemagglutination assay (HA) and hemagglutination inhibition (HI) test to prove APMV-1 serologically. Viral ribonucleic acid was isolated and subsequently reverse transcribed by reverse trasncription reaction followed by amplification by polymerase chain reaction to multiply the cDNA. Microscopically, perivascular cuffing (20%), endoteliosis (75%), and gliosis (75%) were found in the brain. In the lung, an interstitialis pneumonia (50%), lobar pneumonia (5%), and proliferation of pneumosit type 2 (100%) were observed. The most prominent intestinal lesions were catarrhal enteritis (75%) and hemorrhagic necrotizing enteritis (10%). Confirmation of the 20 isolates obtained in this study both serologically and molecularly were positive APMV-1. Moreover PCR results showed that 80% of its amplicon showed a single band and 20% still require some optimizations to get single good bands.
Patological Changes in Liver and Gall Bladder Of Bali Cattle Infected by Fasciolosis I Made Kardena; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; Ida Bagus Windia Adnyana; I Made Sukada; Kadek Karang Agustina; Putu Agus Trisna Kusuma Antara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p02

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Fascioliosis is a parasitic disease that infects ruminants and the disease is widely spread in the world. Fascioliosis caused by Fasciola hepatica and Fasciola gigantica that can cause macroscopic and microscopic lesions in the liver and gall bladder of bali cattle. Samples of bali cattle in Pesanggaran slaughter house that infected with fasciolosis were used in this study. The pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The parasite found in the liver and gall bladder, thickening of bile duct mucous were observed on macroscopic examination. However, in microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy and hyperplasia of the bladder epithelium were occurred.
Hubungan Pengetahuan Gizi Ibu dan Screen Time dengan Kebiasaan Jajan pada Balita Zogara, Asweros Umbu; Pantaleon, Maria Goreti; Nur, Astuti; Nita, Maria Helena Dua; Sine, Juni Gressilda L.; Adi, Anak Agung Ayu Mirah
Muhammadiyah Journal of Nutrition and Food Science (MJNF) Vol 5, No 1 (2024): Muhammadiyah Journal of Nutrition and Food Science (MJNF)
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/mjnf.5.1.13-21

Abstract

Latar Belakang: Konsumsi jajanan dilakukan oleh semua individu, termasuk balita. Konsumsi jajanan secara berlebihan akan berdampak pada kesehatan. Faktor-faktor yang berhubungan dengan konsumsi jajanan pada balita adalah pengetahuan gizi ibu dan screen time. Ibu berpengetahuan gizi baik cenderung memberikan makanan yang sehat dan bergizi kepada anaknya. Penggunaan screen time yang lama akan meningkatkan konsumsi jajanan. Tujuan: menganalisis hubungan pengetahuan gizi ibu dan screen time dengan kebiasaan jajan pada balita. Metode: Penelitian ini dilaksanakan di Kota Kupang pada bulan Maret sampai Juni 2023. Sampel dalam penelitian ini adalah balita berumur 24-59 bulan berjumlah 366 orang. Data penelitian dianalisis menggunakan uji chi square. Hasil: Pengetahuan gizi ibu masih kurang (56,6%) dan screen time pada balita cukup tinggi, yaitu ≥2 jam per hari (77,9%). Hasil uji statistik menunjukkan ada hubungan yang signifikan antara pengetahuan gizi ibu dan screen time dengan kebiasaan jajan pada balita (p value = 0,000). Simpulan: Ada hubungan signifikan antara pengetahuan gizi ibu dan screen time dengan kebiasaan jajan pada balita.
Mammary Tumors in Dogs, Recent Perspectives and Antiangiogenesis as a Therapeutic Strategy: Literature Study Sewoyo, Palagan Senopati; Adi, Anak Agung Ayu Mirah; Winaya, Ida Bagus Oka; Wirata, I Wayan
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.271-287

Abstract

A particular type of tumor that is frequently detected in female dogs who are sexually active is a mammary tumor. Neoplasia results from DNA-based alterations in cell cycle regulating genes. The mammary gland is prone to the formation of tumors due to its dynamic structure. The development of this tumor is supported by numerous variables. It has been recently discovered that there is substantial evidence linking the BRCA2 gene to the process of cancer. Standard examination techniques, such as fine needle aspiration, histopathology, and immunohistochemistry, are used along with ancillary tests to determine the tumor type and degree of malignancy. The primary treatment option for malignant tumors is surgical resection followed by adjuvant chemotherapy; benign tumors necessitate surgical resection as well. Adjuvant therapy options include hormone therapy and non-steroidal anti-inflammatory medications. Tumor tissue undergoes angiogenesis as it grows and develops to accommodate the abundant supply of nutrients. Therefore, angiogenesis-inhibiting therapies can be utilized to halt the growth of tumor cells. A number of antiangiogenic medications are now being studied in clinical settings on humans, and several more are undergoing trials on animals. In addition to pharmaceuticals, viruses may be used as a therapeutic to block tumor angiogenesis.
Co-Authors Agung Arta Putra, I Gusti AGUSTINA SETIA Aida Lousie Tenden Rompis Alberto Agustinho Pereira Da Costa Joao Anak Agung Bagus Bramardipa Anak Agung Bagus Bramardipa Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Aras, Fatmawati Aristawati, I Dewa Agung Ayu Irma Astuti Nur Astuti Nur ASWEROS UMBU ZOGARA Bayu Setiabudi Benu, Lolang Loriana Bire, Ienoliski Rohi BORO, REGINA MARIA Chiharu Hongo Costa, Santa Luciana LDV da da Costa, Santa Luciana Diaz Vera Dewa Ayu Agus Sri Laksmi Dzikri Nurma'rifah Takariyanti Eisaku Tamura Elyda . Fedri Rell Ferbian Milas Siswanto Gusti Ayu Yuniati Kencana Hernomoadi Huminto Hidayatullah, Lalu Syarif I Dewa Made Adhiwitana I Gede Hendra Prasetya Wicaksana I Gusti Agung Arta Putra I GUSTI AGUNG AYU AMBARAWATI I Gusti Agung Dewi Sarihati I Gusti Agung Gede Putra Pemayun I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Kadek Swastika I Ketur Berata I Ketut Berata I Ketut Berata, I I KETUT ELI SUPARTIKA I Ketut Eli Supartika I Ketut Suastika I Made Anom Sutrisna Wijaya I Made Galih Diparayoga I Made Kardena I Made Kardena, I I Made Merdana I Made Sudarmaja I Made Sukada i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Wayan Wirata I Wayan Wita Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Oka Winaya Ida Bagus Oka Winaya, Ida Ida Bagus Windia Adnyana Jane Austen Peni Jayawardhita, Anak Agung Gde Juni Gressilda L. Sine Kadek Karang Agustina Komang Andika Purnama Komang, Nadia Eprillia Sary Darma Ni L.Sin, Juni Gressilda Lies Parede Hernomoadi Loaloka, Meirina S Loaloka, Meirina S Loaloka Luh Made Sudimartini M. Farhan Al Ma'arif Mada, Maria Magdalena Ki Maria F Vinsensia D P Kewa Niron Maria Goreti Pantaleon Marissa Divia Dayanti Marissa Divia Dayanti Marson, Fransiska Gratia Sonita Meirina S Loaloka Meirina Sulastri Loaloka Mia Monica Nadira, Laras Ayu Ni Luh Putu Eka Diarthini Nita, Maria Helena Dua Nofantri, Lidia Nopita Wati, Ni Made Nur Baiti Palagan Senopati Sewoyo Palagan Senopati Sewoyo Pinem, Nuh Lasjuardi Purnasari, Maria Elisabeth Putra, I Putu Werdikta Jayantika Putu Agus Trisna Kusuma Antara Putu Ayu Asri Damayanti Putu Henrywaesa Sudipa Rahmat Grahadi Regina Maria Boro Ruwa idah, Ruwa Sampelimbong, Marissa Santa Luciana da Costa Sartika, Nola Alfien Sartika, Nola Alfieni Sembiring, Anita Ch Sewoyo, Palagan Senopati Sine, Juni Gressilda L Sine, Juni Gressilda L. Tampubolon, Yandri Putra Lumatas Wijaya, Anak Agung Gede Oka Wira, Amar Yasunobu Matsumoto Yeni Ratna Sari