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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Online Mahasiswa (JOM) Bidang Pertanian Jurnal Ijtimaiyya JURNAL KEPEMIMPINAN DAN PENGURUSAN SEKOLAH Jurnal SOLMA JMKSP (Jurnal Manajemen, Kepemimpinan, dan Supervisi Pendidikan) Jurnal Studia Insania JISIP: Jurnal Ilmu Sosial dan Pendidikan AL MUNIR : Jurnal Komunikasi dan Penyiaran Islam Jurnal PGSD Besaung : Jurnal Seni Desain dan Budaya PRODU-Prokurasi Edukasi (Jurnal Manajemen Pendidikan Islam) Jurnal At-Taghyir : Jurnal Dakwah dan Pengembangan Masyarakat Desa Al-Muaddib : Jurnal Kajian Ilmu Kependidikan Airlangga Journal of Innovation Management Jurnal Pengabdian UNDIKMA Griya Journal of Mathematics Education and Application Majalah Ilmiah Tabuah: Ta`limat, Budaya, Agama dan Humaniora Indo-MathEdu Intellectuals Journal Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan Makara Journal of Science Proceeding ISETH (International Summit on Science, Technology, and Humanity) JURNAL FIRNAS Jurnal Masyarakat Religius dan Berwawasan Indev: Literasi Media Dakwah dan Pengembangan Masyarakat Alfuad: Jurnal Sosial Keagamaan Journal of Multidisciplinary Inquiry in Science, Technology and Educational Research Indonesian Journal of Jamu Global Review of Tourism and Social Sciences COGNITIVISM: Journal of Education Research and Development Batanang : Jurnal Psikologi Berita Biologi
Tri Yuliani, Tri
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Makara Journal of Science

 1 
Hemoglobin-Modified Core–Shell Fe3O4@Au Nanostructures for the Electrochemical Detection of Acrylamide Saepudin, Endang; Yuliani, Tri; Nasution, Mochammad Arfin Fardiansyah; Khalil, Munawar; Hong, Jong Wook; Ivandini, Tribidasari Anggraningrum
Makara Journal of Science Vol. 25, No. 3
Publisher : UI Scholars Hub

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Abstract

In this study, electrochemical detection of acrylamide using hemoglobin (Hb)-modified core–shell Fe3O4@Au nanostructures was conducted. Fe3O4 nanoparticles (~4.9 nm) and core–shell Fe3O4@Au (5.0–6.4 nm) nanostructures were successfully synthesized by the thermal decomposition method. Electrochemical investigation revealed that the optimum amount of Hb of 2 mg/mL could be immobilized in 0.1 M acetate buffer solution (pH = 6). Moreover, the detection of acrylamide using Fe3O4@Au/Hb was evaluated by the cyclic voltammetry technique. A linear calibration curve (R2 = 0.98) in the concentration range of 0.1 to 1.0 µM could be achieved with an estimated limit of detection, limit of quantification, and sensitivity of 0.136 µM, 0.453 µM, and 0.4411 µA/µM, respectively. Furthermore, the developed biosensor exhibited high selectivity in the presence of ascorbic acid, melamine, and caffeine. The developed biosensor was applied to the detection of acrylamide in coffee samples and validated using the standard high-performance liquid chromatography (HPLC) method. The concentration of acrylamide in coffee samples was determined to be 37.450 and 35.377 ppm using electrochemical measurement and HPLC, respectively.
Co-Authors AA Sudharmawan, AA Adhli, Aulya Anas, M. Annisa, Mella Armizawati, Armizawati Arnis En Yulia Atifah, Yusni Budiman, Novi Damri Damri Desanawita, Desnawita Deskasari, Sri Desnawita, Desnawita Deswita Deswita Dewijanti, Indah Dwiatmi Dewiyanti, Indah Dwiatmi Dwi Wahyuni Efnaliza, Efnaliza Elvarisna, Elvarisna Endang Saepudin, Endang Fatrisna, Yuli Febrina, Rifka Filailla, Euis Flora, Dian Gusnita, Fitria Gustianda, Nana Hanafi, Beni Putra Hong, Jong Wook Hutabarat, Rinto Indah D Dewijanti, Indah D Irawadi, Hermen Irwandi Irwandi Irwandi Irwandi Iswadi Iswadi Ivandini Tribidasari Anggraningrum Kusno Listyani, Erlin Mahmuda Mahmuda, Mahmuda Maimori, Romi Marissa Angelina Marlina, Inda Marsena, Miftahul Maryani, Wela Mastanora, Refika Megawati - Meliana Sari, Meliana Melva Louisa Minarti, M Mochamad Muchson Muhammad Kristiawan Munawar Khalil Nadia Gustina, Nadia Nafrialdi Nafrialdi Nasution, Mochammad Arfin Fardiansyah Nursiswanto, Heri Nuryasni Nyoto Suseno Oktavia, Lidya Fransiska Oktri Permata Lani Putri, Santi Virdaus Ramadhani, Nana Refnawati, Refnawati Riki Rikarno, Riki riswanto riswanto Rizna Triana Dewi Rr. Forijati -, Rr. Forijati S, Banjarnahor S D Saputra, Septia Irnanda Sari Fitri Sari, Rahmi Sari, Windi Yunita Sesmira, Meli Shanty Komalasari, Shanty Sim, Kezia Jeconia Sinuraya, Bachtiar Siswahyudianto Subagyo, Subagyo Sugiono Sugiono Syafriani, Velly Teni Ernawati Triana, Mamy Vivian Soetikno Wahyu, Fadjri Wahyuni, Roza Sri Wawaimuli Arozal Wendra, Zetria Yani, Nurmaningsih Yarnita, Yetti Yovica, Alia Yulinda, Desni Zahara, Sri Zulham Zulham