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All Journal Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture Science Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Fisheries & Marine

Nunuk Dyah Retno Lastuti

Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia 60116

Author-ID : 676897

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Deteksi Cryptosporidium canis pada Anjing di Kota Surabaya (CRYPTOSPORIDIUM CANIS DETECTION IN DOGS IN THE CITY OF SURABAYA) Romy Muhammad Dary Mufa; Nunuk Dyah Retno Lastuti; Fedik Abdul Rantam; Lucia Tri Suwanti; Endang Suprihati; Didik Handijatno; Mufasirin Mufasirin
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.954 KB)

Cryptosporidiosis is a disease caused by Cryptosporidium spp. protozoan parasites and are zoonotic. Cryptosporidium canis is the main species that infects dogs. Transmission of C. canis in dogs to humans is possible. This study aims to detect microscopic C. canis infection based on morphology and molecularity using the Polymerase Chain Reaction (PCR) in dogs in Surabaya City. A total of 80 diarrhea dog feces samples were taken from Animal Hospitals and animal clinics in several areas in the Surabaya City, then added potassium dichromate and stored at 4OC. Detection was made of the presence of Cryptosporidium spp. oocysts microscopically which is then confirmed by molecular examination using the PCR method. The results showed 40 positive samples containing Cryptosporidium spp., oocysts, with a size of 2-6 ?m. Ten samples from the total positive sample of Cryptosporidium spp. oocysts by microscopic examination, with the PCR test there were seven positive samples of C. canis. Based on the results of the study it can be concluded that the species that causes Cryptosporidiosis in dogs in Surabaya City is C. canis. The high cases of Cryptosporidiosis in dogs can be a warning to be able to prevent Cryptosporidium spp. infections, especially in pets that have the potential as a reservoir in spreading disease.

Deteksi Molekuler Blastocystis sp. pada Babi Terinfeksi di Kabupaten Tabanan dan Badung, Provinsi Bali, Indonesia (MOLECULAR DETECTION OF BLASTOCYSTIS INFECTION IN PIGS AT TABANAN AND BADUNG DISTRICT, BALI PROVINCE, INDONESIA) Doohan Mahendra; Lucia Tri Suwanti; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Endang Suprihati; Wiwik Misaco Yuniarti; Ni Komang Aprilina Widisuputri
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.288 KB)

This study aims to identify morphology and molecular of Blastocystis infection in pigs in the Tabanan and Badung District, Bali Province, Indonesia. 100 samples of pig stool were taken, fifty samples from the Tabanan and fifty samples from Badung District. Blastocystis sp. isolated and identified microscopically and confirmed by polymerase chain reaction (PCR) as a molecular identification method. 63% (63/100) Blastocystis is found in pig feces from these two District by microscopic detection. Positive samples were found 27 samples from Badung and 36 samples from Tabanan. Five samples were taken from each District conducted a confirmation test using the PCR method and found 60% (6/10) positive samples, two samples from Badung and four samples from Tabanan. The results of this study indicate that in healthy pigs, they also contain parasites that have the potential to spread disease to humans. Therefore, the high incidence of Blastocystis in pigs can be an alarm or warning to prevent Blastocystis infection from livestock that can potentially to be a reservoir in the spread of disease.

Respons Sitokin Interferon Gamma Terhadap Derajat Infeksi Skabies pada Kelinci Amirotul Azhimah; Nunuk Dyah Retno Lastuti; Thomas Valentinus Widiyatno; Lucia Tri Suwanti; Poedji Hastutiek
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Skabies merupakan penyakit kulit menular yang disebabkan oleh tungau Sarcoptes scabiei. Peran IFN-? terhadap reaksi hipersensitivitas skabies dengan tingkat keparahan yang berbeda belum banyak dilaporkan. Penelitian ini bertujuan untuk mendeteksi peran sitokin IFN-? pada proses peradangan dan tingkat kerusakan kulit akibat derajat infeksi skabies pada kelinci sehingga dapat diketahui lebih lanjut mengenai reaksi patogenesis skabies pada kelinci. Sebanyak 24 ekor kelinci yang secara alami terinfeksi S. scabiei digunakan dalam penelitian ini. Penelitian ini mengelompokkan hewan coba menjadi empat kelompok berdasarkan luasan lesi dan ketebalan krusta akibat infeksi skabies, yaitu: kelompok kontrol, skabies ringan, skabies sedang, dan skabies berat. Setiap kelompok dilakukan pemeriksaan imunohistokimia menggunakan antibodi IFN-?. Hasil penelitian menunjukkan bahwa derajat infeksi skabies berpengaruh terhadap ekspresi sitokin IFN-? yang ditunjukkan dengan intensitas perubahan warna kecoklatan yang berbeda pada jaringan kulit kelinci yang terinfeksi skabies ringan, skabies sedang, dan skabies berat. Uji statistika dengan uji Kruskal Wallis menunjukkan bahwa terdapat perbedaan yang nyata pada ekspresi sitokin IFN-? terhadap skabies ringan, skabies sedang, dan skabies berat P<0,05). Simpulan dari penelitian ini yaitu semakin berat derajat infeksi skabies, ekspresi IFN-? semakin meningkat.

Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya Fransiska Cicilia Beka; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Koesdarto Koesdarto; Mufasirin Mufasirin; Endang Suprihati; Dyah Ayu Kurniawati
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk mendeteksi E. suis pada babi yang dipotong di Rumah PotongHewan (RPH) Pegirian, Surabaya. Sebanyak 63 sampel feses babi dikoleksi dari RPH Pegirian Surabaya. Deteksi morfologis Entamoeba spp. dilakukan dengan metode apung (analisis parasitologi). Selain itu, penentuan spesies E. suis dilakukan dengan PCR menggunakan primer Esuis764 (5’-ATC AAA TCA ATT AGG CAT AAC TA-3’) dan Esuis765 (5’-AAT TAA AAC CTT ACG GCT TTA AA-3’) dan prodak PCR yang diharapkan adalah 320 bp. DNA dari sampel positif diekstraksi dengan DNAzol. PCR dilakukan pada beberapa sampel positif yang dipilih secara acak dari analisis parasitologi. Hasil penelitian menunjukkan 57,14% (36/63) sampel secara morfologis positif Entamoeba sp. Hasil penentuan spesies berdasarkan analisis molekuler menemukan bahwa protozoa yang menginveksi babi di Rumah Potong Hewan Pegirian, Surabaya adalah E. suis.

Perbedaan Larva Stadium Kedua dan L2 Toxocara canis pada Jaringan Mencit Menggunakan Scanning Electron Microscopy (THE DIFFERENCES BETWEEN SECOND STAGE LARVAE AND L2 TOXOCARA CANIS ON MICE TISSUE BY USING SCANNING ELECTRON MICROSCOPY) Vindo Rossy Pertiwi; Kusnoto Kusnoto; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Mufasirin Mufasirin
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Toxocariasis is one of zoonosis diseases that caused by Toxocara spp. that is Toxocara canis. Toxocara canis has several stages until it can infect animals and humans, namely the egg stage, larvae first stage (L1), larvae second stage (L2), larvae third stage (L3) to adult worms. Studies about the L2 and L2 tissue of T. canis found in paratenic hosts using Scanning Electron Microscopy (SEM) have not been widely performed. Some of the causes include L2 being not easily to found and identified, so research rarely raises the ultrastructural morphology of L2 and L2 tissues. Knowledge about the ultrastructural morphology of L2 and L2 tissue of T. canis worms is very important to determining the diagnosis, especially the etiological diagnosis. The purpose of this study is to detected morphology of L2 and L2 tissues of T. canis using SEM. Samples from this study is faeces of dogs that infected with toxocariasis and the digestive tract of dogs obtained from dog slaughter houses. The sample is an adult worm of T. canis; the female worm is dissected and taken uterus to collect worm eggs. The results of this study on microscopic and optilab examination showed a difference between L2 and L2 tissue that the length of L2 hatched from embryonic eggs was 390 ìm and with a width of 23.4 ìm at the midpoint of the body. Larvae second stage length from the infected somatic tissue is 410 ìm and the width is 22.5 ìm at the midpoint, and then difference in dorsal lip, cuticles, body ring, cervical alae, buccal capsul, tail.

Morfometri dan Ultrastruktur Cacing Fasciola gigantica pada Sapi Donggala dan Sapi Bali di Kabupaten Berau, Kalimantan Timur (MORPHOMETRY AND ULTRASTRUCTURAL OF FASCIOLA GIGANTICA WORM IN DONGGALA AND BALI CATTLE IN BERAU DISTRICT, EAST KALIMANTAN) Muhammad Rofi' Prasetya; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Setiawan Koesdarto; Kusnoto Kusnoto; Muchammad Yunus
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Fasciolosis is one of the endemic parasitic diseases in Indonesia. Fasciola gigantica is the main species found to infect livestock, especially beef cattle. The purpose of this study was to analyze the morphometry and identify the ultrastructure morphology of the F. gigantica worm isolated from beef cattle in Berau abattoir. The F. gigantica worms were isolated from two cattle breeds, namely Donggala cattle and Bali cattle. Worm was stained by using carmine methode to analyze the morphometric of the worm and scanning electron microscopy to identify ultrastructure morphology. Morphometric of the worm was analysed with the one sample t-test and multidimensional scaling statistical methods. The results of the morphometric analysis showed that F. gigantica from both breeds had significant differences (P<0.05) between the parameters and based on multidimensional scaling analysis had similarities with F. intermedia from Iran. Based on scanning electron microscopy examination showed that F. gigantica from the Donggala cattle breed was infected with type 1 of F. gigantica while F. gigantica from the Bali cattle breed was infected with type 2 of F. gigantica based on ventral sucker, oral sucker, and spine. It can be concluded that F. gigantica found in beef cattle in Berau was type 1 F. gigantica (Donggala cattlebreed) and type 2 F. gigantica (Bali cattle breed) and similar with F. intermedia from Iran.

Co-Authors Abdullah Abdullah Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Afiat Berbudi Agnes Theresia Soelih Estoepangestie Agustina, Firanda Ahmad Ghiffari Aldi Hamdani Amalia Rosydinasari Rosydinasari Amirotul Azhimah Amirul Muslim Amrullah Anam Al Arif Anord Charles Nkuba Bambang Sektiari Lukiswanto Chairil Anwar Chaniful Fuadi, Muhammad Dadik Rahardjo, Dadik Dadik Raharjo Damayanti, Tita Desiandura, Kurnia Dian Ayu Permatasari Didik Handijatno Diyah Ayu Candra Doohan Mahendra Dwi Ananda, Novita Dwi Handayani Dyah Ayu Kurniawati E Djoko Poetranto Ellza Agatha Damayanti Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri Fadila Zikri Amanda Fatmawati, Mira Fatmawati Fedik Abdul Rantam Fransiska Cicilia Beka Gandul Atik Yuliani Gita Dwi Prasasty Gunanti Mahasri Hana Eliyani Hartono Hartono Hidajanti, Nove Inda Astri Aryani Ira Sari Yudaniayanti Kadek Rachmawati Koesdarto Koesdarto Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lianny Nagoi Lilik Maslachah Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luluk Tri Astuti Lutfiah Annisa Billa Marchelia Arifiandani Masdiana C Padaga Maudika Pamela Fiorenza Meles, Dewa Ketut Meyreta Doti Alcaterana Miftahurrizqiyah Miftahurrizqiyah Mirni Lamid Mochamad Lazuardi Mochammad Amin Alamsjah Moh. Ilham Rizkul Ulum Muchammad Yunus Mufasirin Muhamad Amin Muhammad Athoillah Sholahuddin Muhammad Chaniful Fuadi Muhammad Fahmi Abdillah Muhammad Rofi' Prasetya Mustofa Helmi Effendi Ni Komang Aprilina Widisuputri Nisa’ Rachmaningtyas Putri Nove Hidayati Nusdianto Triakoso P Kumaladewi Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastutiek Prasetya, Muhammad Rofi' Pratiwi, Arif Prawasa, Ramadhiniyanti Putri Alif Rahmi Sugihartuti Retno Bijanti Rizhar Eman Karunia Akbar Rochmah Kurnijasanti Romy Muhammad Dary Mufa Sapto Andriyono Setiawan Koesdarto Soeharsono Soeharsono Sri Agus Sudjarwo Sri Subekti Sunarso, Agus Suzanita Utama Taufik Tri Laksono Tjuk Imam Restiadi Trifena Pristi Anindyta Veena Vasheene Vijaya Kumar Vindo Rossy Pertiwi Warsito, Sunaryo Hadi Widi Nugroho Widiyatno, Thomas Valentinus Win Darmanto Wiwik Misaco Yuniarti Wurlina, W Yeni Dhamayanti Yunus, Muchammad Yunus, Muchammad

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