Claim Missing Document
Check
Articles

Genetic relatedness and antimicrobial resistance of Staphylococcus aureus in goats with subclinical mastitis, North Sumatra, Indonesia SHINTA MUTIA RAMBE MANALU; LISNAWITA LISNAWITA; NEVY DIANA HANAFI; LUTHFI AZIZ MAHMUD SIREGAR
Biodiversitas Journal of Biological Diversity Vol. 27 No. 2 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270233

Abstract

Abstract. Manalu SMR, Lisnawita, Hanafi ND, Siregar LAM. 2026. Genetic relatedness and antimicrobial resistance of Staphylococcus aureus in goats with subclinical mastitis, North Sumatra, Indonesia. Biodiversitas 27 (2): d270233. https://doi.org/10.13057/biodiv/d270233. Staphylococcus aureus is widely recognized as a dominant etiological agent of mastitis in dairy goats and is also regarded as a zoonotic pathogen capable of causing a broad spectrum of human infections, including severe and life-threatening conditions. Despite the routine use of antimicrobial agents to control S. aureus infections, increasing resistance to multiple antibiotic classes has substantially reduced treatment effectiveness. This study investigated the isolation and molecular characterization of S. aureus associated with subclinical mastitis in dairy goats (Capra hircus) from Deli Serdang and Serdang Bedagai Regencies, North Sumatra, and evaluated antimicrobial susceptibility against commonly used antibiotics in regional livestock production. Descriptive analysis supported by graphical visualization and phylogenetic reconstruction was applied to assess distribution patterns and genetic relatedness, while statistical comparisons of antimicrobial resistance proportions between regencies were performed using Fisher’s exact test. This study confirms S. aureus as the most frequently bacterium isolated bacterium in CMT-positive samples based on phenotypic and molecular analyses. High resistance to β-lactam antibiotics was observed in both regions. In Deli Serdang, resistance to amoxicillin and penicillin G was detected in 73.8% (31/42) and 71.4% (30/42) of isolates, respectively. Similarly, in Serdang Bedagai, resistance to amoxicillin and penicillin G was found in 69.0% (20/29) and 58.6% (17/29) of isolates. In contrast, tetracycline showed a higher susceptibility profile in both regions. No statistically significant differences were observed between the two regencies for amoxicillin, penicillin G, or tetracycline resistance (p>0.05). Partial 16S rRNA analysis placed the isolates within S. aureus clade, showing high sequence similarity to reference strains reported from Brazil and India. Collectively, these findings provide essential baseline evidence supporting rational antimicrobial selection and continued resistance surveillance in caprine mastitis management.
The Effect of Adding Batak Onion (Allium chinense G. Don) to Drinking Water on Digestibility in Broiler Chickens Aditya Pangestu; Peni Patriani; Nevy Diana Hanafi; Wahyudi Himawan Sutanto; Bayriel Dudeq Opok
Jurnal Peternakan Integratif Vol. 14 No. 02 (2026): Jurnal Peternakan Integratif
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jpi.v14i02.26436

Abstract

Indonesia population growth has driven an increase in the demand for chicken meat.This study aimed to determine the effect of adding Batak onion (Allium chinense G.Don) decoction to drinking water on feed intake and digestibility of dry matter and organic matter in broiler chickens and mortality. The research was conducted experimentally using a Completely Randomized Design (CRD) with four treatments: T0 (without Batak onion), T1 (15%), T2 (20%), and T3 (25%), each with five replications. The results showed that the addition of Batak onion decoction has no significantly affected (P>0.05) on on dry matter intake, dry matter digestibility, organic matter digestibility, or mortality. However, digestibility values remained within optimal ranges and there is no mortality compare to treatment without Batak onion. The best treatment was found at the 15% concentration, which resulted in the highest dry matter intake. The addition of Batak onion in drinking water shows potential as a natural alternative to improve digestive efficiency in broiler chickens.
The Effect of Providing Moringa Leaf Flour (Moringa oleifera) in Feed as a Feed Additive on Increasing the Performance of Super Village Chickens (Gallusgallus domesticus L. variation joper) Winda Saragih; Carlos Marianus Manalu; Sayed Umar; Nevy Diana Hanafi
Journal of Tropical Animal Science and Technology Vol. 7 No. 1 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Science Study Program, Faculty of Agriculture, Science and Health, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v7i1.8846

Abstract

Joper chickens are breeds of kampung chickens, Bangkok chickens, or other local breeds of chickens separated from purebred chickens. In addition to genetic factors and feed management, the use of natural constituents as feed additives can affect the meat quality of super hens. Feed additives are ingredients that do not include food substances that are added to the stove in small amounts and are aimed at spurring growth and increasing feed efficiency. The existence of various studies in the selection of feed raw materials that produce quality feed additives that are easy to use and cheap to meet the needs of super rural chickens are moringa leaves. The design used in this research was a Completely Randomized Design (CRD) with 4 treatments and 5 replications. The treatment consisted of T0 (Ration does not contain Moringa leaf flour), T1 (Ration contains 6% Moringa leaf flour), T2 (Ration contains 9% Moringa leaf flour), T3 (Ration contains 12% Moringa leaf flour), The parameters observed were feed consumption, body weight gain and feed conversion. The results showed that Moringa leaf flour had a significant effect on feed consumption, body weight gain and feed conversion. The best results were obtained in treatment T1, namely the ratio containing 6% Moringa leaf flour, which was able to increase feed consumption by 367.46 grams, body weight gain by 88.62 grams and feed conversion ratio by 4.16.
Determinants of the Performance Index in Closed-House Broiler Farms Partnered with PT. Karya Semangat Mandiri in North Sumatra Raja Aman; Nevy Diana Hanafi; Ma’ruf Tafsin; Desti Prestasi Zendrato
Jurnal Agripet Volume 26, No. 2, October 2026
Publisher : Animal Husbandry Department, The Faculty of Agriculture, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v26i2.2187

Abstract

This study examined environmental, farmer, labor, and managerial factors associated with the performance index (PI) of closed-house broiler farms partnered with PT. Karya Semangat Mandiri in North Sumatra. An observational census survey included 66 eligible partner farmers in Batu Bara (21), Simalungun (25), and Karo (20) from August 2025 to March 2026. Each farmer record summarized two to five production cycles. Descriptive statistics and multiple linear regression with ten explanatory variables were used. The mean PI was 446.07, 424.31, and 402.62 in Batu Bara, Simalungun, and Karo, respectively. The regression model explained 49.0% of PI variation (adjusted R2 = 0.398; F = 5.293; P<0.001). Owner involvement was the only statistically significant adjusted predictor (B = 15.039; P = 0.016). Its score averaged 4.41±0.62 on a five-point scale, with an observed range of 3.00–5.00. A one-point higher score was associated with a 15.039-point higher PI, conditional on the other predictors. The findings support prioritizing owner supervision, production-record review, and worker guidance within partnership support. The observational design and environmental multicollinearity limit causal interpretation.
The Effect of Adding Batak Onion (Allium chinense G. Don) to Drinking Water on Digestibility in Broiler Chickens Aditya Pangestu; Peni Patriani; Nevy Diana Hanafi; Wahyudi Himawan Sutanto; Bayriel Dudeq Opok
Jurnal Peternakan Integratif Vol. 14 No. 02 (2026): Jurnal Peternakan Integratif
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jpi.v14i02.26436

Abstract

Indonesia population growth has driven an increase in the demand for chicken meat.This study aimed to determine the effect of adding Batak onion (Allium chinense G.Don) decoction to drinking water on feed intake and digestibility of dry matter and organic matter in broiler chickens and mortality. The research was conducted experimentally using a Completely Randomized Design (CRD) with four treatments: T0 (without Batak onion), T1 (15%), T2 (20%), and T3 (25%), each with five replications. The results showed that the addition of Batak onion decoction has no significantly affected (P>0.05) on on dry matter intake, dry matter digestibility, organic matter digestibility, or mortality. However, digestibility values remained within optimal ranges and there is no mortality compare to treatment without Batak onion. The best treatment was found at the 15% concentration, which resulted in the highest dry matter intake. The addition of Batak onion in drinking water shows potential as a natural alternative to improve digestive efficiency in broiler chickens.
Co-Authors A Hakim Daulay Achmad Sadeli Achmad Sadeli Achmad Syahdani Ade Wildani Isma Aditya Pangestu Aisyah, Nurmi Amin Pugut Prayogo Amin Pugut Prayogo Anisa Yuliandha Ari Ashari Harahap Arie Andry Sembiring Arif Martien Putra Arja, Maulidani Armyn Hakim Daulay Armyn Hakim Daulay Aulia FIkri Ariq Putra Banjarnahor, Evi Reka Banjarnahor, Evi Reka Bayriel Dudeq Opok Carlos Marianus Manalu Daulay, Armyn Hakim Deddy Rolan Siregar Desti Prestasi Zendrato Desti Prestasi Zendrato Desy Valentina P E Mirwandhono Edhy Mirwandhono Edhy Mirwandhono Edi Bigborn Nugraha Capah Elisa Julianti Erwandi Simbolon Eva Morani Silaban Fajar Bahari Ginting Frinando S Pakpahan Gema Pirngadi Gultom Ginting, Rendell Herbert Hamdan Hamdan Hamdan Hamdan . Hamdan Hamdan Hans Yoppie Sinaga Harahap, Ari Ashari Hasnudi Hasnudi Hasnudi . Hasnudi Hasnudi Henuk, Yusuf Leonard iin melani harahap Indria Ningsih Irwansyah Febri Silalahi Iskandar Sembiring Iskandar Sembiring Iskandar Sembiring Jekson Pasaribu Karunia Simanungkalit keke rejeki sembiring Lesli Desora Br Tarigan LISNAWITA LISNAWITA Lumban Tobing, Ersan Riaji Lusiyana Wanti Sihite LUTHFI AZIZ MAHMUD SIREGAR M Tafsin M Tafsin Ma&#039;ruf Tafsin Ma'ruf Tafsin Ma'ruf Tafsin Maeika Putri Sitepu Manalu, Carlos Marianus Maradona Barutu maruf tafsin tafsin Masrayani Pulungan Mastopan Ma`ruf Tafsin Ma’ruf Tafsin Ma’ruf Tafsin Ma’ruf Tafsin Ma’ruf Tafsin Ma’ruf Tafsin Ma’ruf Tafsin Musa Seno Ibrahim Mustapa Zahri Ritonga Mustapa Zahri Ritonga Nurainun Harahap Nurtati Natalia Nababan Nurul Hasanah Silalahi Nurzainah Ginting Peni Patriani Purnama Ketaren Rahayu, Risma Raja Aman Rayswe Dumaris Sihombing Reza Prabudi Rico Rifki Yudiar Rina Daniati Lumbangaol Riyena Talarosa Pohan Saragih, Winda Saragih, Winda Fransisca Sayed Umar sayed umar Sayed Umar SHINTA MUTIA RAMBE MANALU Sigit Sepriadi Simson Sianturi Siregar, Julinda Siti Zulaiha Sitorus Sonya Elisabeth Silaen Sri Fajar Ayu Sri Haryani Sitindaon Susiana Nababan Syahril Bancin Tafsin, Ma’ruf Tafsin, Ma’ruf Tanjung, A. M. Tri Hesti Wahyuni Tri Hesti Wahyuni Tri Hesti Wahyuni Tri Hesti Wahyuni Tri Hesti Wahyuni Tri Hesty Wahyuni Tri Hesty Wahyuni Tuty Elvi Farida Ukurta Pinem Umar, Sayed Unchu Maiwanda Usman Budi Veronica Sinamo Wahyudi Himawan Sutanto Wike Sujana Wilda Rifki Winda Saragih Wulandari Yoppi Iskandar Yunilas . Yusni Khairani Tampubolon Yusrizal Fachri