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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Perikanan dan Kelautan ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Pendidikan Biologi Undiksha BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Pro Food Celebes Biodiversitas : Jurnal Sains dan Pendidikan Biologi ANNALES BOGORIENSES Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Martabe : Jurnal Pengabdian Kepada Masyarakat CARADDE: Jurnal Pengabdian Kepada Masyarakat Jurnal TAMBORA JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Community Development Journal: Jurnal Pengabdian Masyarakat BIOMA : Jurnal Biologi dan Pembelajarannya
Manguntungi, Baso
Progam Studi Pendidikan Biologi, Fakultas Keguruan Dan Ilmu Pendidikan, Universitas Sulawesi Barat
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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ISOLASI, KARAKTERISASI, DAN UJI ANTIMIKROBA PADA BAKTERI ASAM LAKTAT DARI USUS Trigona Spp. Sama, Yusuf; Hasgun; Depparinding, Rekianto; Alfiana; Rahman, Auliyah; Manguntungi, Baso; Sari, Arlinda Puspita; Ariandi; Nurmuliayanti, Nurmuliayanti
BIOMA: Jurnal Biologi dan Pembelajarannya Vol 5 No 2 (2023): BIOMA: Jurnal Biologi dan Pembelajarannya
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/bioma.v5i2.3120

Abstract

Lebah Trigona spp. merupakan lebah yang tidak mempunyai sengat (stinglees bee) dan memiliki kemampuan memproduksi propolis tinggi sebagai mekanisme pertahanan diri dan sebagai antibiotik terhadap bakteri ataupun cendawan. Pada penelitian in dilakukan proses isolasi bakteri asam laktat (BAL) dari saluran pencernaan Trigona spp. asal Sumbawa yang dilanjutkan dengan karakterisasi BAL, dan uji antimikroba metabolit BAL terhadap bakteri Salmonella thypi dan Staphylococcus aureus. Hasil isolasi diperoleh 20 isolat bakteri, kemudian dilakukan skrining BAL melalui uji katalase dan pewarnaan gram, diperoleh 18 isolat BAL. Kandidat isolat BAL dilkulturkan selama 24 jam, kemudian mengisolasi ekstrak kasar metabolit BAL. Hasil isolasi ekstrak kasar metabolit BAL diujikan ke bakteri S. thypi dan S. aureus selama enam jam menggunakan metode difusi agar, kemudian dilakukan pengukuran zona bening pada jam kedua, keempat dan keenam untuk mengetahui efektivitas daya hambat/antimikroba metabolit BAL. Hasil uji menunjukkan metabolit isolat PRT 9 memiliki kemampuan tertinggi dibandingkan dengan 17 isolat BAL lainnya dalam menghambat pertumbuhan bakteri S. thypi dengan luas zona hambat sebesar 6,7±0,471 mm dan S. aureus sebesar 3,0±0 mm.
Potential Probiotic Yeasts of the Pichia Genus Isolated from ‘Dadih’, a Traditional Fermented Food of West Sumatra, Indonesia Chihombori, Tatenda Calvin; Mustopa, Apon Zaenal; Astuti, Rika Indri; Mutiara, Ilma; Refli, Redoyan; Umami, Rifqiyah Nur; Fatimah; Irawan, Herman; Ekawati, Nurlaili; Trinugroho, Joko P; Akmaliyah, Rizna; Chairunnisa, Sheila; Amani, Febriyanti Nur; Manguntungi, Baso; Hertati, Ai; Mamangkey, Jendri
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.320-340

Abstract

Fermented buffalo milk, known as dadih, serves as a reservoir of potential probiotic yeasts. Over the past two decades, probiotic yeasts have gained increasing attention in both basic and clinical sciences due to their health benefits. This study aimed to isolate and characterize probiotic yeasts from dadih. Yeasts were isolated using yeast Extract, peptone, and dextrose (YPD) medium, and molecularly identified through 18S-rRNA sequencing. Probiotic potential was assessed by evaluating resistance to acidic pH, bile salts, proteolytic, lipolytic, and hemolytic activities. Secondary metabolites produced during fermentation were tested for antimicrobial properties. GBT30 and GBT37 isolates were selected based on their superior performance in probiotic property assays for further analysis. Molecular identification revealed these isolates as Pichia occidentalis (GBT30) and Pichia kudriavzevii (GBT37). Both strains demonstrated in vitro survivability under simulated gastrointestinal conditions and exhibited antimicrobial activity. Whole-genome sequencing of P. kudriavzevii GBT37 identified a genome size of 10,906,850 base pairs, distributed across four chromosomes with a GC content of 38.26%. Notably, secondary metabolite biosynthesis genes were located on contig 7. In addition, 26 probiotic-related genes, including GSY1, HSC82, HSP104, TPS1, ARN1, FLO1, ALA1, SIR2, and others, were identified in P. kudriavzevii GBT37, indicating its potential as a probiotic yeast. The traditional fermentation process of dadih offers probiotic yeasts with promising health benefits, supporting its potential as a functional food.
The Gene Analysis and Probiotic Potential Characterization of Pseudomonas alcaligenes SG03 Manguntungi, Baso; Rusmana, Iman; Mustopa, Apon Zaenal; Meryandini, Anja
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.1039-1052

Abstract

AHPND causes up to 80% of shrimp mortality, severely impacting Indonesia's aquaculture. Rising antibiotic resistance necessitates sustainable alternatives. This study investigates the probiotic potential of Pseudomonas alcaligenes SG03, a bacterium with unique genomic traits, to address antibiotic resistance and promote animal health. Genome analysis revealed a 6.17 Mb genome with 4,446 protein clusters, including genes for carbohydrate metabolism (26.63%), amino acid metabolism (19.93%), and energy metabolism (10.88%). Key probiotic-related genes, such as acid resistance (rpoS, actP), salinity tolerance (gshA, cysK), antibiotic resistance (vanB, gyrA), and metal resistance (copZ, zwf), were identified. In vitro, assays evaluated probiotic properties under conditions mimicking the shrimp gastrointestinal tract. Osmo-tolerance tests showed optimal growth at 10% glucose, with a significant drop in viability at higher concentrations. Autoaggregation increased progressively, reaching 0.78 at 18 hours and 0.13 at 48 hours. Optimal growth occurred at 30°C, with a 2.5-fold increase in optical density compared to 25°C. NaCl tolerance peaked at 2% (20.33×1012 CFU at 48 hours), while bile salt tolerance was highest at 0.1% (27.00×1012 CFU at 48 hours). pH tolerance was optimal at pH 5 (20.00×1012 CFU at 48 hours). Antioxidant (53.00%) and anti-inflammatory (60.33%) activities peaked after 48 hours. Phenotypic antibiotic resistance was observed against Tetracycline, Amoxicillin, Cefixime, Streptomycin, and Chloramphenicol, with proteolytic activity but no lipolytic or hemolytic properties. These findings highlight P. alcaligenes SG03’s potential as a probiotic in aquaculture to support shrimp health and reduce antibiotic dependency. Future studies should focus on in vivo validation and formulation development.
Antibacterial and Antifungal of β-sitosterol Isolated from Hydroid Aglaophenia cupressina Lamoureoux Against Xanthomonas campestris and Fusarium oxysporum Johannes, Eva; Manguntungi, Baso; Tuwo, Mustika; Litaay, Magdalena; Mustopa, Apon Zaenal; Vanggy, Leggina Rezzy
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 30, No 2 (2025): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.30.2.192-202

Abstract

This study aimed to isolate and characterize β-sitosterol from the marine hydroid Aglaophenia cupressina Lamoureoux and evaluate its antibacterial and antifungal activities against Xanthomonas campestris and Fusarium oxysporum. β-Sitosterol was extracted, purified, and identified using spectroscopic techniques, including infrared and nuclear magnetic resonance spectroscopy. Antimicrobial activity was assessed through agar diffusion method to determine its inhibitory effects on bacterial and fungal growth at varying concentrations. β-sitosterol is a crystalline compound, with a melting point of 138-139°C, consistent with the reported range for pure β-sitosterol, indicating high purity. Infrared (IR) spectroscopy revealed key functional groups, including a hydroxyl group at 3433 cm⁻¹, C-O stretching at 1050 cm⁻¹, and aliphatic hydrocarbon chain vibrations at 2956, 2938, and 2869 cm⁻¹. The C=C stretching at 1634 cm⁻¹ and C-H bending at 1465 cm⁻¹ confirmed its unsaturated sterol structure. ¹H NMR spectroscopy further confirmed the structure with characteristic methyl and olefinic proton signals. The antibacterial activity of β-sitosterol against Xanthomonas campestris showed a concentration- and time-dependent effect, with the highest efficacy observed at 60 ppm, demonstrating potential as a natural antibacterial agent. Additionally, its antifungal activity against Fusarium oxysporum revealed both fungistatic and fungicidal effects, with lower concentrations exhibiting fungistatic behavior and higher concentrations displaying fungicidal activity, thereby offering versatility for both fungal inhibition and eradication. This dual action, combined with its well-characterized molecular structure, positions β-sitosterol as a promising candidate for further development as an antimicrobial compound. The findings underscore the accuracy of the identification process and highlight β-sitosterol's potential in pharmaceutical and agricultural applications, particularly in combating bacterial and fungal infections.
Peningkatan Kapasitas dan Manajemen Agroeduwisata Desa Batulaya dengan Teknologi Hidroponik dan Vertikal Garden Berbahan Sampah Plastik Nurdin, Rizaldi; Ariandi; Haloho, Ruth Dameria; Masniawati, Andi; Muis, Nurmuliayanti; Manguntungi, Baso
CARADDE: Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 2 (2024): Desember
Publisher : Ilin Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31960/caradde.v7i2.2570

Abstract

This community service aims to enhance agro-edutourism management in Batulaya Village through training on hydroponic and vertical garden technology, utilizing plastic waste as a planting medium. The agro-edutourism in this village has great potential but faces challenges in managing limited land and high levels of plastic waste. The training program involved 65 participants, including local farmers, village youth, and agro-edutourism managers. A qualitative method was used to collect data through questionnaires and observations, which were then analyzed using thematic analysis. The results show that the training successfully improved participants' knowledge and skills in applying hydroponic and vertical garden technology. A total of 85% of participants were able to understand the basic concepts of the technology, and 70% could utilize plastic waste as a planting medium. The activity concludes that training and improving agro-edutourism management based on hydroponics and vertical gardens in Batulaya Village not only succeeded in enhancing the community's technical capacity but also provided innovative solutions to environmental issues. However, challenges in accessing resources and technology need to be addressed to ensure the sustainability of this program.
PEMBERDAYAAN KELOMPOK TANI SIAMASEI DESA LEGO MELALUI TEKNOLOGI BIOFERTILIZER UNTUK PENINGKATAN PRODUKSI BAWANG MANDAR Puspita Sari, Arlinda; Ariandi, Ariandi; Muis, Nurmuliayanti; Manguntungi, Baso
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 6, No 12 (2023): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v6i12.4511-4520

Abstract

Kelompok Tani Siamasei merupakan salah satu kelompok tani yang mewadahi petani bawang mandar di Desa Lego. Saat ini banyak petani bawang mandar yang mulai beralih pada komoditas pertanian lain sebab menganggap pertanian bawang mandar kurang memberi keuntungan. Padahal bawang mandar merupakan salah satu komoditas khas Sulawesi Barat yang banyak digunakan dalam berbagai olahan makanan. Hal ini disebabkan oleh berbagai permasalahan yang dihadapi oleh petani bawang mandar, diantaranya menurunnya produktivitas bawang mandar akibat penurunan kualitas lahan pertanian. Berdasarkan permasalahan tersebut, maka solusi yang ditawarkan adalah dengan kegiatan pelatihan dan pendampingan sistem pertanian berbasis teknologi biofertilizer. Tujuan pengabdian ini adalah agar peserta mengetahui teknologi pupuk hayati berbasis mikroorganisme yang dapat membantu petani untuk meningkatkan produktivitas bawang mandar tanpa merusak kondisi lahan. Pengabdian ini meliputi beberapa tahap kegiatan antara lain FGD, pembuatan starter dan biofertilizer, pelatihan dan pendampingan, serta monitoring dan evaluasi. Hasil yang diperoleh dari kegiatan pengabdian ini adalah diproduksinya starter mikroba yang dapat digunakan oleh petani sebagai stok untuk membuat biofertilizer secara mandiri. Selain itu melalui kegiatan pelatihan dan pendampingan, petani memperoleh informasi baru mengenai teknologi pemanfaatan mikroba dalam pembuatan pupuk hayati. Dari tahap evaluasi diperoleh hasil bahwa sebanyak 88.5% peserta berpendapat bahwa kegiatan pelatihan telah berjalan dengan baik.
Co-Authors A. Masniawati Adha Ardinata, Rian Afgani, Chairul Anam Ai Hertati, Ai Akmaliyah, Rizna Alfiana Amani, Febriyanti Nur Amrullah, Shafwan Andi Dewi Rizka Ainulia Makerra Anika Prastyowati Anita Ervina Anja Meryandini Apon Zaenal Mustopa Apon Zaenal Mustopa Apon Zaenal Mustopa Ariandi Ariandi Ariandi, Ariandi Arlinda Puspita Sari Asia Arifin Asmawati, Rian Adha Ardinata Muhammad Al Azhar Bugi Ratno Budiarto Chaidir, Riri Rimbun Anggih Chairunnisa, Sheila Chihombori, Tatenda Calvin Dahliah Depparinding, Rekianto Dwi Ratna Sari Ekawati, Nurlaili Endang Saepudin Eva Johannes Eva Johannes Fadhil Zil Ikram Fahri Sinulingga Fatimah Hasgun Iman Rusmana Intan Dwi Pratiwi Irawan, Herman Irlan izzul islam Jendri Mamangkey Jumriani, Siti Khadijah Alliya Fidien Khotibul Umam Kurniawan Eka Putra Tegar Aprilian1 Kus Dianawati Kustiariyah Tarman La Ode Fitradiansyah Leggina Rezzy Vanggy Leggina Rezzy Vanggy Leggina Rezzy Vanggy Lukman Azis Magdalena Litaay Maritsa Nurfatwa Masni Masni Mietra Anggara Mohammad Rofiqi Muh. Chaerul Anwar Muhammad Abdi Mahesa Muhammad Fajri Al-Fateeh Muhammad Ilham S Muhammad Rajib Muhammad Rizaldi Trias Jaya Putra Nurdin Muhammad Rofiq Muis, Nurmuliayanti Musdalipah Mustika Tuwo, Mustika Mutiara, Ilma Nairfana, Ihlana Naning Sufiyanti Nur Hilal A. Syahrir Nurdin, Rizaldi Nurlaili Ekawati Nurmuliayanti Muis Nurmuliayanti, Nurmuliayanti Nurul Amilia Putra, Kurniawan Eka Rahmadinah, Nurul Afifah Rahman, Auliyah Ramlah Ramlah Ramlah Refli, Redoyan Rian Adha Ardinata Muhammad Al Azhar Asmawati Riana Anggraeny Ridwan Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Robby Erlangga Ruth Dameria Haloho Sama, Yusuf Saputri, Dinar Suksmayu Shasmita Irawan Sri Wahyuni Suharli, Lili Sulastri, Ani Syamsul Arifin Tegar Aprilian Tiara Fitri Erinanda Trinugroho, Joko P Ulfa Febiana Whatin Vanggy, Leggina Rezzy Wahyullah Wahyullah Widya Dwi Kusuma Yulianti Yulianti Yusran Yusran Zainal