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All Journal ACTA VETERINARIA INDONESIANA Jurnal Ilmiah Mahasiswa Veteriner Jurnal Pendidikan Dasar Albacore : Jurnal Penelitian Perikanan Laut Jurnal Natural Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Jurnal Medika Veterinaria
Sari, Wahyu Eka
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Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Clinical Outcomes of Autologous Serum Eye Drops in Treating Corneal Ulceration in a Domestic Cat: A Case Base Study Eka Putri, Rahmi; Hilmi, Mulkiah; Rahmi, Ulfa; Zamzami, Rumi Sahara; Etriwati, Etriwati; Erwin, Erwin; Sugito, Sugito; Syah, Nada Yulian; Ferdian, Riyan; Sari, Wahyu Eka; Safitri, Jessica Anggun
Jurnal Medika Veterinaria Vol 19, No 2 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i2.46171

Abstract

Corneal ulceration is a frequent ophthalmic disorder in domestic cats that can cause ocular discomfort, visual impairment, and serious complications if left untreated. Conventional medical management often provides limited support for epithelial regeneration, leading to the need for alternative therapeutic options. This case study reports the clinical outcome of using autologous serum eye drops (ASEDs) in the treatment of corneal ulceration in a domestic cat. A cat presented with severe epiphora, conjunctival hyperemia, and a centrally located corneal ulcer confirmed by fluorescein staining. Autologous serum was prepared by weekly collection of 6 mL of blood from the dorsalis antebrachii vein, followed by centrifugation and aliquoting into sterile eye drop bottles stored at 4 C. The serum was applied topically twice daily, accompanied by supportive therapy consisting of vitamin A supplementation, multivitamin injections, and an Elizabethan collar to prevent self-trauma. Clinical scoring (03) was used to monitor epiphora, conjunctival hyperemia, and corneal opacity over the treatment period. Marked improvement was observed within 14 days: epiphora and conjunctival hyperemia resolved completely, while corneal opacity improved from moderate to mild, with fluorescein staining becoming negative, indicating complete epithelial closure. No adverse effects were recorded during therapy. This case highlights the potential of autologous serum eye drops as a safe and effective adjunct treatment for feline corneal ulceration, warranting further investigation in larger cohorts to establish standardized protocols for veterinary ophthalmology
In Vitro Analysis of Antibacterial Activity of Black Seed Oil Against Salmonella Typhi Zulfayanto, Indri; Vanda, Henni; Hambal, Muhammad; Sari, Wahyu Eka; Muttaqien, Muttaqien
Jurnal Medika Veterinaria Vol 19, No 2 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i2.48983

Abstract

Salmonella sp. is one of the bacteria that causes diarrhea in animals and humans, especially in naive animals whose immune systems have not yet been formed. In relation to the emergence of antibiotic resistance against Salmonella typhi, it is necessary to develop an innovation regarding alternative medicines that use herbal medicines as antibacterials, one of which is black seed oil which contains active substances such as thymoquinone. Nigella sativa, commonly known as black seed, has long been used as herbal medicine with many benefits. This study aimed to determine the antibacterial activity of black seed oil against S. typhi bacteria in vitro. This study used S. typhi ATCC 14028, which had been previously re-identified using Gram staining. The black seed oil for the antibacterial test was prepared with three concentrations, 45%, 70%, and 100%, with dimethyl sulfoxide (DMSO) as a negative control and ciprofloxacin as a positive control. Black seed oil's antibacterial test was carried out using the Kirby Bauer method. The data was analysed descriptively. The results showed that black seed oil was not effective in inhibiting the growth of S. typhi, where the inhibition zone formed was less than 5 mm. This is because the antigenic structure of S. typhi has Vi antigens or capsular antigens which are made of polysaccharide polymers found outside the cell to protect the bacteria from outside influences. Therefore, the active ingredients in the black seed oil was unable to reach the site of action on the bacteria so they failed to inhibit or kill the S. typhi bacteria. It can be concluded that the black seed oil used in this study was not effective in inhibiting the growth of S. typhi ATCC 14028
Antidiabetic Test of Ethanolic Extract of Ant Nest Plants (Myrmecodia sp.) Against the -Glucosidase Enzyme In Vitro Daniel, Daniel; Isa, Muhammad; Hasan, Muhammad; Sari, Wahyu Eka; Rosmaidar, Rosmaidar; Aisyah, Siti; Athaillah, Farida; Frengki, Frengki
Jurnal Medika Veterinaria Vol 18, No 1 (2024): J.Med.Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i1.37431

Abstract

The use of plants, one of the biological sources, as an alternative medicine for degenerative diseases is increasingly being studied. Usually, the biological effects of these plants come from the secondary metabolite components contained therein. Ant nest is one of them which is reported to contain compounds belonging to the class of flavonoids, tannins, and polyphenols. This research was conducted in vitro by observing the antidiabetic potential of the ethanolic extract of ant nests against the -glucosidase enzyme following the modified "Wako Pure Chemical Industries Ltd., Japan" protocol. The results showed that the inhibitory power of -glucosidase of the extract was stronger than that of the quercetin control with IC50 values of 10.59 g/mL and 38.09 g/mL, respectively. These results proved that the ethanolic extract of ant nest had very strong -glucosidase inhibitory property. Predictive analysis of the pharmacokinetics and toxicity of the bioactive compounds contained in the ant nest extract showed that rutin has similar characteristics to acarbose as a first-line anti-diabetic drug of choice through an -glucosidase inhibitor mechanism, so rutin is worthy of consideration for development as an anti-diabetic candidate.
Antibacterial Activity of Tamarind Leaf-Based Shampoo Against Staphylococcus aureus and Pseudomonas aeruginosa, Candida albicans, and Malesezia globose Sari, Wahyu Eka; Sadri, Hidayatus; Hasanah, Lailatul; Yona, Safetri; Triyuliani, Ririn; Maghfirah, Aura; Frengki, Frengki; Riandi, Lian Varis; Riady, Ginta
Jurnal Medika Veterinaria Vol 17, No 2 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i2.35271

Abstract

Skin and feather diseases are common in cats. The main causative agents of these diseases are bacteria and fungi, including Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Malassezia globosa fungi which are one of the fungi that play a role in the formation of dandruff. Preventive measures that can be carried out are grooming or bathing cats using special shampoos. The use of natural-based shampoos is an alternative to reduce the side effects of using chemical-based shampoos. Like herbal shampoo from tamarind leaf extract. The samples used in the study were tamarind leaves and isolates of S. aureus and P. aeruginosa, C. albicans and M. globosa. This research used completely randomized design (CRD) model with three treatment groups (2% and 20% for extract, tamarind leaf extract shampoo) for antibacterial test. Positive controls consisted of vancomycin 30 mg for S. aureus, and gentamicin 10 mg for P. aeruginosa, and distilled water as negative control. For antifungal test, there were three different concentrations (16%, 8%, and 4%), tamarind leaf extract shampoo, one positive control (zinc pyrithione 1%) and one negative control (distilled water) with two replications. Data was analyzed by measuring the inhibition zone formed. The results of the inhibition zone on S. aureus were 6.03 mm (2%) and 13.48 mm (20%), and for tamarind leaf extract shampoo was 9.54 mm, categorized as weak. For P. aeruginosa, the inhibition zone was 6.61 for 2%, and 19.78 for 20% (categorized as strong), tamarind leaf extract shampoo was 10.23 mm (weak category. For C. albicans, inhibition zone formed at a concentration of 4% was 6 mm, 8% was 6 mm, 16% was 6 mm, and tamarind leaf extract shampoo was 6 mm, (weak category). For M. globosa at a concentration of 4% was 0 mm, 8% was 6 mm, 16% was 6 mm, and tamarind leaf extract shampoo was 12.32 mm (strong category). It can be concluded that the ethanolic extract of tamarind leaf had inhibition activity on the growth of S. aureus, P. aeruginosa, C. albicans, and M. globosa.
Total Plate Numbers and Coliforms in The Processing of Milkfish Without Spines (Chanos Chanos) in Household Indusrty in Banda Aceh Nurliana, Nurliana; Aftari, Nailul; Sutriana, Amalia; Asmilia, Nuzul; Sari, Wahyu Eka
Jurnal Medika Veterinaria Vol 17, No 2 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i2.34843

Abstract

Microbial contamination of fish has the potential to cause product quality degradation and cause fishborne disease. Microbial contamination can occur at any stage of fish processing. This study aimed to determine the total plate count and coliform at each stage process of milkfish (Chanos chanos) boneless. Milkfishes were obtained from three processing stage, namely raw material (fresh fish) stage, the fish bone removal stage and the baking fish stage. The parameters observed were the number of colonies growing as total plate count and coliform as the most probable number (MPN). The results showed that the highest ALT contamination was found at the bone removal stage with an average of 1.7x104 CFU/g but this figure did not exceed the standard limit and the lowest was at the firing stage with an average of 1.2x103 CFU/g. The highest coliform was found in the fresh fish stage, namely 129 MPN/g and the lowest was 3 MPN/g. From this research, it can be concluded that the total microbial plate number does not exceed the SNI 7288: 2009 standard and the coliform exceed the SNI 7388: 2009 standard.
EFFECT OF Nigella sativa AS FEED ADDITIVE ON ORGANOLEPTIC, WATER, PROTEIN, AND CHOLESTEROL CONTENT OF BROILER CHICKEN MEAT WITH HEAT STRESS CONDITION Vanda, Henni; Nurliana, Nurliana; Sugito, Sugito; Ferasyi, Teuku Reza; Sari, Wahyu Eka; Hanafiah, M.; Akram, Saydul; Daulay, Dina Khairani; Paula, Wita
Jurnal Kedokteran Hewan Vol 18, No 2 (2024): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i2.37355

Abstract

This study aimed to determine the physical properties, organoleptic, proximate value, and cholesterol level of broiler meat given black cumin (Nigella sativa) as feed supplement and exposed to heat stress. This study used 15-day old chicken (DOC) broilers that were adapted for 14 days and treated on day 15 to day 28. This study used a completely randomised design (CRD) consisting of 5 treatment groups: P0 (commercial feed); P1 (commercial feed + heat stress 34-35 ); P2 (commercial feed + black cumin 4% + heat stress 34-35); P3 (commercial feed + black cumin 6% + heat stress 34-35 ); P4 (commercial feed + black cumin 8% + heat stress 34- 35 ). Feeding was given twice a day at 07.00 WIB and 17.00 WIB ad libitum. Heat stress was given from 10.00 - 15.00 WIB every day for 14 days. The chicken breast and thigh meat were collected on day 29 for analysis. The statistical analysis showed that feed supplement containing black cumin had no effect (P0.05) on the pH value, drip loss, texture, protein content, and structure of broiler meat given exposure to heat stress, but had effect (P0.05) on the organoleptic colour, smell and texture, and cholesterol level of broiler meat. Based on these results, it can be concluded that feeding with 4%, 6% and 8% black cumin did not change the pH, drip loss, texture value as well as protein and water content of broiler meat given heat stress, but produced better colour and aroma. The administration of 4% cumin can reduce cholesterol levels of broiler meat exposed to heat stress.
THE EFFECT OF AEROB AND ANAEROBIC CONDITION AND ADDITION OF CaCO3 ON THE GROWTH AND ANTIBACTERIAL ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM LAYER HENS INTESTINE AFTER ADMINISTRATED OF AKBISprob Nurliana, Nurliana; Khairunisa, Fauzia; Musni, Nailah; Vanda, Henni; Rasmaidar, Rasmaidar; Hasan, Muhammad; Asmilia, Nuzul; Sari, Wahyu Eka; Isa, Muhammad
Jurnal Kedokteran Hewan Vol 17, No 3 (2023): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i3.20309

Abstract

This study aimed to determine the effect of aerobic and anaerobic incubation, and the addition of CaCO on the growth and antibacterial activity of Lactic Acid Bacteria (LAB) isolated from laying hens intestines (strain ISA Brown) after given 4% AKBISprob. The LAB used in this study was the isolates strains that produced the strongest antibacterial activity with inhibition zone 5 mm. The treatments consisted of two incubations condition (aerobic and anaerobic) with five concentrations of CaCO3 (1%, 2%, 3%, 4%, and 5%). Each treatment consisted of three repetitions. Total Plate Count (TPC) method and the agar diffusion method were used to calculate the amount of LAB and antibacterial activity, respectively. The results showed that anaerobic conditions had a significant effect (P0.05) on the increase of LAB growth, but both of aerobic and anaerobic conditions (P0.05) did not show significant effect on antibacterial activity. The addition of CaCO3 had a significant effect (P0.05) on the increase in growth and antibacterial activity of LAB. Based on the results of the study, it can be concluded that anaerobic incubation can increase the growth of two isolate of LAB isolates (P31S72 and P31H62), however aerobic and anaerobic incubation have no effect on antibacterial activity. In addition, the administration of 3% CaCO3 can increase the growth and antibacterial activity of all LAB isolates.
Recurrent urethral obstruction in a male Persian cat Zamzami, Rumi Sahara; Yuliansyah, Nanda; Ferdian, Riyan; Hasan, M; Sari, Wahyu Eka
The International Journal of Tropical Veterinary and Biomedical Research Vol 9, No 1 (2024): Vol. 9 (1) May 2024
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v9i1.39114

Abstract

Recurrent urethral obstruction (rUO) is a critical condition predominantly affecting male Persian cats, necessitating urgent veterinary care due to its high morbidity and potential mortality. This case report details a five-year-old male Persian cat with a history of five episodes of urethral obstruction, despite neutering after the second incident. The cat presented with symptoms including excessive straining and inappropriate urination. Diagnostic evaluations involved physical examination, urinalysis, urine sedimentation, radiography, and ultrasonography. Laboratory results revealed abnormalities such as elevated leukocyte count, blood, protein, nitrites, and struvite crystals in the urine. Radiographic and ultrasonographic imaging indicated an enlarged and firm urinary bladder with no visible stones, but a thickening of the bladder wall and crystal accumulation were noted. The management strategy included immediate relief of obstruction via catheterization and long-term dietary adjustments, though the cat's habit of consuming non-prescribed food contributed to recurrent episodes. This report highlights the challenges in managing rUO in Persian cats and emphasizes the importance of comprehensive diagnostic procedures, strict dietary management, and ongoing monitoring to prevent recurrence. The findings contribute to the existing literature on feline lower urinary tract diseases (FLUTD) and offer insights for veterinarians in clinical practice, suggesting that prolonged catheterization and strict adherence to a specialized urinary diet may reduce recurrence rates and improve outcomes for affected cats.
Co-Authors . Darniati Aftari, Nailul Akram, Saydul Amalia Sutriana Andi Novita Athaillah, Farida Awaluddin Awaluddin Azhari Azhari budianto panjaitan cut nila thasmi Daniel Daniel Darmawi Darmawi Daulay, Dina Khairani Erina Erina Erina Erina erma yusintha ramdhania Erwin Erwin Etriwati E Fadlah, Iga Faisal Jamin Ferdian, Riyan Frengki Frengki, Frengki Ginta Riady Hasanah, Lailatul Hasriati Hasriati, Hasriati Hasyimi, Adyar Henni Vanda Hennivanda Hennivanda Hilmi, Mulkiah Ismail Ismail ismail ismail ismail Jessica Anggun Safitri Khairunisa, Fauzia M Daud AK M Hasan M Nur Salim M. Hanafiah Maghfirah, Aura Mahdi Abrar Morizka Wianda Muhammad Hambal Muhammad Hambal Muhammad Hasan Muhammad Isa Muhammad Jalaluddin, Muhammad Musni, Nailah Muttaqien Muttaqien, Muttaqien Nanda Yulian Syah Noni Zakiah NURLIANA NURLIANA Nuzul Asmilia Panjaitan, Tasya Adilla Paula, Wita R. Rusli Rahmadhini, Vivi Rahmi Eka Putri Rahmi, Ulfa Rasmaidar Rasmaidar Rastina Rastina Razali Daud5 Riandi, Lian Varis Rizki, Maurina Rosmaidar Rosmaidar, Rosmaidar Rumi Sahara Zamzami Sadri, Hidayatus Safika S, Safika Samsul Bahri Siti Aisyah Sugito - Sukardi Sukardi Syah, Nada Yulian T Reza Ferasyi Teuku Reza Ferasyi Teuku Zahrial Helmi Triva Murtina Lubis Triyuliani, Ririn Waridah Waridah, Waridah Wilyo Hendro Fauzan Yona, Safetri Zakya Ardi Zamzami, Rumi Sahara Zulfayanto, Indri