Claim Missing Document
Check
Articles

Found 20 Documents
Search

Striped catfish oil and turmeric extract reduces inflammation in metabolic syndrome rats Mambaya, Helvi Desi; Muniroh, Muflihatul; Afifah, Diana Nur; Anjani, Gemala; Pramono, Adriyan; Sulchan, Mohammad
International Journal of Public Health Science (IJPHS) Vol 14, No 2: June 2025
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v14i2.24984

Abstract

Metabolic syndrome is a growing global health problem. Long-term treatment for metabolic syndrome causes side effects. Therefore, the use of nutraceuticals could also be considered. This study analyzed the effect of the administration of striped catfish oil and turmeric extract on tumor necrosis factor-α (TNF-α) levels and homeostasis model assessment of insulin resistance (HOMA-IR) in metabolic syndrome rats. Metabolic syndrome was induced in rats by administration of high fat fructose diet (HFFD) containing 3 g pork fat (15%), 2 g duck egg yolk (10%), 15 g standard diet (75%), and 2 ml fructose (1%). Thirty rats were randomized into five groups: C1 (normal control group), C2 (metabolic syndrome control group without treatment), P1 (striped catfish oil at 0.08 ml/200 g BW/day), P2 (turmeric extract at 5.04 mg/kg BW/day), P3 (combination of striped catfish oil at 0.08 ml/200 gBW/day and turmeric extract at 5.04 mg/kg BW/day). There was a significant decrease (p<0.05) in TNF-α levels and HOMA-IR in treatment groups (P1, P2, P3) compared to C2. The P3 group had the lowest TNF-α levels. Treatment groups had the same potential effect in reducing HOMA-IR. Striped catfish oil, turmeric extract, and their combination reduce inflammation and insulin resistance in metabolic syndrome rats.
COMPARISON OF THE EFFECTS OF CORE EXERCISE AND BALANCE STRATEGY EXERCISE ON WALKING SPEED AND FRAILTY LEVEL IN THE ELDERLY Utami, Arini; Muniroh, Muflihatul; yuniati, Renni
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 1 (2025): APRIL 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i1.38507

Abstract

Penuaan menyebabkan perubahan fisiologis dan biomekanik, termasuk penurunan otot, berkurangnya panjang langkah, kecepatan berjalan lebih lambat, dan peningkatan risiko jatuh. Studi ini membandingkan efek latihan inti dan keseimbangan pada kecepatan berjalan dan tingkat kelemahan pada orang tua. Ini menggunakan Desain Kelompok Kontrol Pra dan Pasca Tes dengan 40 responden yang dibagi secara acak menjadi dua kelompok. Satu kelompok melakukan latihan inti, sementara yang lain berlatih latihan strategi keseimbangan, keduanya dilakukan tiga kali seminggu selama empat minggu. Kecepatan berjalan diukur menggunakan tes kecepatan berjalan 4 meter, dan kelemahan dinilai dengan Indeks Kelemahan. Hasil penelitian menunjukkan bahwa pada kelompok inti, kecepatan berjalan pasca-tes rata-rata 1,27 m/s, meningkat dari 0,98 m/s (p=0,00), sedangkan pada kelompok keseimbangan, meningkat dari 1,03 m/s menjadi 1,14 m/s (p=0,004). Skor Indeks Kelemahan kelompok inti menurun dari 1,55 menjadi 0,6 (p=0,00), sedangkan skor kelompok keseimbangan menurun dari 1,6 menjadi 1,4 (p=0,046). Tidak ditemukan perbedaan signifikan antara kelompok dalam peningkatan kecepatan berjalan (p=0,051), tetapi latihan inti secara signifikan mengurangi kelemahan lebih banyak daripada latihan keseimbangan (p=0,00). Sebagai kesimpulan, kedua jenis latihan tersebut meningkatkan kecepatan berjalan dan mengurangi kelemahan pada lansia. Namun, meskipun latihan inti dan latihan keseimbangan memiliki efek yang sama pada kecepatan berjalan, latihan inti secara signifikan lebih efektif dalam menurunkan tingkat kelemahan.
Penyuluhan Osteoporosis dalam Upaya Pencegahan Komplikasi Tulang pada Pasien Talasemia Mayor di Kabupaten Rembang dan Grobogan Widyastiti, Nyoman Suci; Retnoningrum, Dwi; Ariosta, Ariosta; Nency, Yetty Movieta; Ngestiningsih, Dwi; Partiwi, Rina; Muniroh, Muflihatul; Naviati, Elsa; Afriana, Rizky Dyas
Journal of Dedicators Community Vol 7, No 3 (2023)
Publisher : Universitas Islam Nahdlatul Ulama Jepara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34001/jdc.v7i3.3577

Abstract

Background Osteoporosis is a complication that often occurs in thalassemia major patients. This complication is still not widely known. This community service program aims to increase the knowledge of families of major thalassemia patients in Rembang Regency and Grobogan Regency regarding the complications of osteoporosis in thalassemia patients. Methods Education about osteoporosis, risk of osteoporosis in thalassemia patients, and prevention of osteoporosis. Extension method with presentation and discussion. The activity was attended by 20 thalassemia families in the Rembang Regency and 21 families of thalassemia patients in the Grobogan Regency. Before and after counseling, pretest and posttest questionnaires were completed to measure the knowledge of families of thalassemia major patients regarding bone complications in thalassemia patients. Results and Discussion Nearly half (46.34%) of respondents did not know the risk of osteoporosis in thalassemia. After counseling, 82.93% of respondents knew the risk of osteoporosis in thalassemia, but there were still 17.07% of respondents who still did not know this risk. Conclusion There is a significant increase in family knowledge of thalassemia major patients about the risk of osteoporosis in thalassemia. Further efforts to increase knowledge are needed with written information media (leaflets) given to families of thalassemia major patients
The Relationship Between Hair Zinc Levels, Feeding Patterns, and Infectious Diseases on The Incidence of Stunting in Children Aged 24-59 Months in The City of North Jakarta, Province of DKI Jakarta Fitry, Febriana Ramadhani; Margawati, Ani; Syauqy, Ahmad; Muniroh, Muflihatul; Kartini, Apoina
Eduvest - Journal of Universal Studies Vol. 4 No. 10 (2024): Journal Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v4i10.1749

Abstract

Globally, stunting is a severe public health concern, especially in low- and middle-income nations like Indonesia. It is typified by stunted growth and development due to recurrent infections and chronic malnutrition. North Jakarta has a 19.8% stunting incidence, according to the 2023 Indonesian Health Survey. This indicates the critical need for additional research into the factors contributing to stunting, such as hair zinc levels, dietary habits, and infectious infections. This study employs an observational case-control design conducted in North Jakarta in March 2024. Stunting incidence and hair zinc levels are significantly correlated (p=0.000), affecting 87.5% of children with low hair zinc levels. Stunting is also correlated considerably (p=0.000) with incorrect feeding patterns; 75.7% of children who exhibit these patterns are stunted. On the other hand, there was no statistically significant correlation (p=0.806) between the incidence of stunting and a history of infectious illnesses. The mother's level of education, the child's age, gender, and family income did not significantly correlate with stunting.
Striped Catfish Oil and Turmeric Extract-Reduce Inflammation and Insulin Resistance on Metabolic Syndrom: A Review Mambaya, Helvi Desi; Muniroh, Muflihatul; Sulchan, Muhammad; Afifah, Diana Nur
Jurnal Penelitian Pendidikan IPA Vol 10 No SpecialIssue (2024): Science Education, Ecotourism, Health Science
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10iSpecialIssue.7440

Abstract

Metabolic Syndrome is continuing to grow worldwide. Indonesia is no exception. High-calorie diets and physical inactivity trigger several pathways involved in metabolic syndrome. These include inflammation and insulin resistance. Associated with type 2 diabetes mellitus, cardiovascular disease, and death, metabolic syndrome is important. There is therefore a need for early intervention to reduce the complications of this disease. Striped catfish (Pangasius hypophthalmus) is one of the freshwater fish farmed in Indonesia. Turmeric (Curcuma longa Linn.) is also widely used in Indonesian food preparations. This review aims to focus on striped catfish oil and turmeric extract that positively affect inflammation and insulin resistance in the intervention of metabolic syndrome. This review shows that striped catfish oil contains omega-3 fatty acids (EPA and DHA), while turmeric extract contains flavonoids, tannins, and saponins. Based on the results of the review, it is known that bioactive compounds found in catfish oil and turmeric extract can be used as nutraceutical ingredients to intervene in metabolic syndrome.
Exploring the effects of ursodeoxycholic acid-probiotic combination on Lactobacillus and liver enzyme levels for cholestasis management: An experimental study Nur, Muhammad Hasbi; Lestari, Endang Sri; Budiono, Bernadus Parish; Prasetyo, Sigit Adi; Muniroh, Muflihatul
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 1, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss1.art10

Abstract

Background: Cholestasis arises from compromised bile secretion due to hepatocyte dysfunction, leading to liver impairment. Available treatments show limited efficacy, with ursodeoxycholic acid (UDCA) being a primary option. Cholestatic conditions influence gut microbiota; therefore, probiotic therapy emerges as a potential approach. Objective: This investigation aimed to evaluate the impact of combined administration of probiotics and UDCA on Lactobacillus levels, as well as the levels of the enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in rats with restricted common bile ducts .Methods: A total of 35 male Sprague Dawley rats were randomly assigned to 7 groups, each comprising 5 members: K1 (healthy control), K2 (negative control with cholestasis), K3 (positive control with cholestasis given UDCA), K4 (cholestasis given 36 mg probiotics), K5 (cholestasis given 18 mg probiotics and UDCA), K6 (cholestasis given 36 mg probiotics and UDCA), and K7 (cholestasis given 54 mg probiotics and UDCA). The treatment duration was 21 days, during which blood samples were collected for AST and ALT analysis. Lactobacillus count was determined by quantitative polymerase chain reaction (PCR) analysis of fecal samples.Results: When UDCA and probiotics were given together in three different dosages, the Lactobacillus count significantly increased (p<0.05) compared to the other groups. Furthermore, compared to the other treatment groups, the UDCA-probiotic combination group exhibited noticeably lower AST and ALT values.Conclusion: Combining UDCA and probiotics elevated Lactobacillus count and decreased AST and ALT levels in cases of cholestasis more effectively than single therapy.
Exploring the remarkable effect of ursodeoxycholic acid and Allium sativum combination on MMP-9 and TIMP-1 levels in cholestatic rat’s model Sekartaji, Anindhita Dyah; Prasetyo, Sigit Adi; Muniroh, Muflihatul
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 3, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss3.art5

Abstract

Background: Cholestasis is a disturbance in the production or flow of bile that causes excessive accumulation of bile fluid and damage in the liver. Chronic liver damage will lead to liver fibrosis. Matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloprotease-1 (TIMP-1) play a critical role in liver fibrosis formation. Although ursodeoxycholic acid (UDCA) and Allium sativum extracts have long been renowned for improving liver function, their combination in alleviating liver fibrosis through the MMP-9 and TIMP-1 inhibitory pathways has yet to be studied.Objective: This study was conducted to assess the efficacy of UDCA and Allium sativum extract combination in altering MMP-9 and TIMP-1 levels, which are the main factors in the progression of liver fibrosis.Methods: We performed an experimental study with a post-test-only control group on 36 Sprague-Dawley rats, which were randomly grouped into healthy, negative, positive, and three treatment groups with UDCA (13.5 mg) and graded doses of Allium sativum extract (3.6 mg; 7.2 mg; and 14.4 mg). Cholestasis induction was done by a choledochal duct ligation, and treatment was given for 14 days. Levels of MMP-9 and TIMP-1 were analyzed after treatment.Results: Combining UDCA and Allium sativum improved the MMP-9 levels significantly in cholestatic rats, compared to administrating UDCA only (p <0.05). Combining UDCA and Allium sativum increased the TIMP-1 level (p < 0.05). Although the MMP-9 level results were under our existing hypothesis, TIMP-1 results showed surprising results. Matrix metalloproteinase-9 levels were strongly negatively correlated with TIMP-1 levels, with a r-value= 0.981.Conclusion: Combining Allium sativum and UDCA alleviates liver fibrosis progression through lowering levels of MMP-9 and increasing levels of TIMP-1.
In vivo assay of Gigantochloa apus shoot extract as biolarvicide for myiasis-causing fly larvae Sadana, Aqsa Aufa Syauqi; Istiadi, Hermawan; Halleyantoro, Ryan; Muniroh, Muflihatul
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 4 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i4.16307

Abstract

The use of synthetic insecticides in treating myiasis is associated with adverse side effects and potential disruption of metabolic systems, prompting interest in natural alternatives. This study investigated the in vivo larvicidal efficacy of Gigantochloa apus bamboo shoot extract, formulated as a spray gel. Twenty-five Wistar rats were randomly divided into 5 groups: a negative control group, a positive control (ivermectin), and 3 treatment groups receiving of 1%, 3%, and 5% G. apus bamboo shoot extract. Myiasis was induced by introducing fly larvae into standardized wounds. Treatments were applied topically twice daily for 32 hr, and larval mortality was assessed every 8 hr. Phytochemical screening and GC-MS analysis revealed the presence of bioactive compounds, including alkaloids, flavonoids, tannins, saponins, and hydrogen cyanide, all of which are known for their larvicidal, neurotoxic, and antiproliferative properties. The 5% extract group showed the highest mortality rate (100%) at 32 hr. The LC50 and LC95 values were determined at 1.43% and 6.01%, respectively. Compared to the standard ivermectin treatment, the 5% extract demonstrated a shorter lethal time and more rapid larval death. Morphological examination revealed darker abdominal segments in the dead larvae, indicating a potential interaction with the digestive tract. These findings indicate that G. apus bamboo shoot extract has potential larvicidal activity and can be an effective natural alternative for treating myiasis.
Supplementation of omega-3 fatty acids can reduce tumor necrosis alpha ( TNF-α) levels and pain intensity in osteoarthritis patients Adengganan, Yogi; Muis, Siti Fatimah; Muniroh, Muflihatul
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 12 ISSUE 6, 2024
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/ijnd.2024.12(6).406-412

Abstract

ABSTRAK Latar Belakang: Osteoarthritis (OA) merupakan penyakit sendi yang paling umum di seluruh dunia, dengan dampak yang kuat pada kesehatan individu dan populasi. Secara global prevalensi OA meningkat sebesar 113,2% dari 247,5 juta pada tahun 1990 menjadi 527,8 juta pada tahun 2019. Prevalensi OA di Indonesia meningkat seiring bertambahnya usia yaitu sebesar 5% pada individu berusia kurang dari 40 tahun, 30% pada usia 40-60 tahun, dan 65% pada usia di atas 60 tahun. Angka kejadian OA lutut relatif tinggi, yaitu 15,5% pada pria dan 12,7% pada wanita. Penyakit ini dapat menyebabkan nyeri kronis, kecacatan, dan menurunkan kualitas hidup penderita. Tingginya kadar sitokin pro inflamasi seperti TNF-α pada penderita OA dapat berperan sebagai faktor utama yang menginduksi kematian krondosit dan menghambat diferensiasi serta proliferasi kondrosit. Penelitian sebelumnya mengatakan bahwa suplementasi omega 3 dapat menurunkan kadar sitokin proinflamasi, memperbaiki nyeri, dan kekakuan fungsi fisik pada individu dengan OA lutut, namun belum ada yang meneliti pengaruh suplementasi omega 3 ini dengan penurunan kadar Tumor Necrosis Factor α (TNF-α).Tujuan: Menganalisis pengaruh  suplementasi asam lemak omega 3 terhadap kadar TNF-α dan intensitas nyeri pada penderita osteoarthritis. Metode: Studi ini merupakan quasi-experimental desain kelompok tunggal dengan rancangan penelitian one group pre-post test without control group design pada 31 pasien. Pengumpulan data dilakukan dengan pengukuran antropometri, pengambilan sampel darah dan pengukuran skor nyeri dilakukan sebanyak dua kali, yaitu sebelum dan sesudah dilakukannya intervensi. analisa TNF-α menggunakan Enzym Linked Immunosorbent Assay (Elisa), intensitas nyeri menggunakan numeric rating scale (NRS). Analisis uji statistik pengaruh suplementasi asam lemak omega 3 terhadap kadar TNF-α dan intensitas nyeri menggunakan uji Wilcoxon.Hasil: Hasil yang diperoleh pada TNF-α dengan nilai P=0,007 dan intensitas nyeri dengan nilai P=0,001 yang menunjukkan hasil yang signifikan.Kesimpulan: Suplementasi asam lemak omega 3 pada penderita OA dapat mengasilkan beberapa mediator anti inflamasi yang mampu menurunkan kadar sitokin pro inflamasi TNF-α dan intensitas nyerisecara siginifikan.KATA KUNCI: asam lemak omega 3 ; tumor necrosis alpha (TNF-α); intensitas nyeri; inflamasi; osteoarthritis ABSTRACTBackground: Osteoarthritis (OA) is the most common joint disease worldwide, strongly impacting individual and population health. Globally, the prevalence of OA increased by 113.2% from 247.5 million in 1990 to 527.8 million in 2019. The prevalence of OA in Indonesia increases with age, namely by 5% in individuals under 40 years, 30% in those aged 40-60 years, and 65% in those over 60 years. The incidence of knee OA is relatively high, namely 15.5% in men and 12.7% in women. This disease can cause chronic pain and disability and reduce the quality of life of sufferers. High levels of pro-inflammatory cytokines such as TNF-α in OA sufferers can act as a major factor in inducing chondrocyte death and inhibiting chondrocyte differentiation and proliferation. Previous studies have shown that omega-3 supplementation can reduce proinflammatory cytokine levels and improve pain and stiffness of physical function in individuals with knee OA. Still, no one has studied the effect of omega-3 supplementation on reducing TNF-α levels.Objectives: To analyze the effect of omega-3 fatty acid supplementation on TNF-α levels and pain intensity in patients with osteoarthritis.Methods: This study is a quasi-experimental single-group design with a one-group pre-post test without a control group design in 31 patients. Data collection was carried out by anthropometric measurements, blood sampling, and pain score measurements twice, namely before and after the intervention. TNF-α analysis using Enzyme-Linked Immunosorbent Assay (Elisa), pain intensity using a numeric rating scale (NRS). Statistical analysis of the effect of omega 3 fatty acid supplementation on TNF-α levels and pain intensity using the Wilcoxon test.Results: The results obtained on TNF-α with a P value = 0.007 and pain intensity with a P value = 0.001 showed significant results.Conclusion: Supplementation of omega-3 fatty acids in OA patients can produce several anti-inflammatory mediators that significantly reduce the levels of pro-inflammatory cytokines TNF-α and pain intensity.KEYWORDS: omega 3 fatty acids; tumor necrosis alpha (TNF-α); pain intensity; inflammation; osteoarthritisReceived: August 14, 2024; Revised: Nov 18 2024; Accepted: Nov 20, 2024; Available online: Nov 30, 2024; Published: Nov 30, 2024.
Soursop Leaf Extract Reduces AST, ALT, Bilirubin Levels, and Liver Damage Scores in Sorafenib-treated Wistar Rats with Hepatocellular Carcinoma Susilaningsih, Neni; Prajoko, Yan Wisnu; Budijitno, Selamat; Prabowo, Erik; Istiadi, Hermawan; Muniroh, Muflihatul; Riwanto, Ignatius
The Indonesian Biomedical Journal Vol 18, No 1 (2026)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v18i1.3883

Abstract

BACKGROUND: Sorafenib, the standard therapeutic agent for advanced hepatocellular carcinoma (HCC), may induce hepatic dysfunction, thereby necessitating adjunctive therapy to mitigate this adverse effect. While preliminary research has suggested that Soursop (Annona muricata) leaves exhibit anti-tumor and hepatoprotective properties, their efficacy in mitigating liver damage associated with sorafenib treatment remains unexplored. This study was conducted to assess the liver-protective effects of soursop leaf extract in Wistar rats receiving sorafenib for HCC treatment.METHODS: Ethanol extract of soursop leaves was prepared using the maceration method. Twenty-nine Wistar rats were divided into five groups: healthy control (HC) group, HCC groups receiving no treatment, sorafenib only, sorafenib + 50 mg/kgBW/day soursop extract, and sorafenib + 100 mg/kgBW/day soursop extract. All groups, except the HC group, were given Diethyl Nitrosamine (DEN) to cause HCC. Following a two-week treatment period, serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were assessed using colorimetric methods, while total bilirubin was assessed using diazo with sulphanilic acid method. From histopathological specimen, relative liver weight was measured and liver damage score was assessed using Hematoxylin and Eosin.RESULTS: Administration of sorafenib resulted in a reduction of AST, ALT, total bilirubin, relative liver weight, and liver damage scores. Furthermore, the combined administration of sorafenib with soursop leaf extract at dosages of 50 and 100 mg/kgBW/day led to a dose-dependent amelioration of these indicators. The most pronounced improvement was observed with the highest dose of soursop extract, which significantly reduced AST, ALT, total bilirubin, relative liver weight, and liver damage scores compared to the sorafenib-only group.CONCLUSION: Soursop leaf extract at 100 mg/kgBW/day effectively reduced AST, ALT, bilirubin levels, and liver damage score in sorafenib-treated Wistar rats with HCC, indicating its hepatoprotective effects. These findings suggest that soursop leaf extract may be a promising adjuvant therapy for mitigating sorafenib-induced hepatotoxicity in HCC treatment.KEYWORDS: Annona muricata, hepatocellular carcinoma, sorafenib, AST, ALT, bilirubin, hepatoprotective