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Journal : FLUIDA

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Optimization of Glucomanan Extraction from Porang Flour with ATPS (Aqueous Two-Phase System) Using Factorial Design Santoso, Budi; Haryadi; Pertiwi , Vani Mutiara; Az-Zahra, Tasya Alya
Fluida Vol. 18 No. 1 (2025): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v18i1.6041

Abstract

Porang tubers are one of the foodstuffs rich in glucomannan content. Glucomannan is widely used in various industrial fields, one of which is pharmaceuticals. To produce pure glucomannan compounds, the method of isolating glucomannan using a two-phase water system (ATPS) solvent has been widely used. One option is isolation with a two-phase water system (ATPS) of inorganic salts ammonium sulfate and ethanol at various concentrations. This study focuses on optimizing the concentration ratio of ammonium sulfate and ethanol for the glucomannan extraction process, carried out with a factorial design and continued analysis with a response surface methodology (RSM) approach. The results of the experimental design at the composition of ammonium sulfate concentration of 17.07% w/w and ethanol of 27.07% w/w showed optimal dry extract acquisition in the two-phase water system (ATPS), with a yield of 81.07%. Characterization of the results of porang flour extract with FTIR produced stretching vibrations of the O-H group at 3427.51 cm-1 and C-O-C (glycosidic bond) at 1020.34 cm-1 and bending vibrations of the -CH functional group at 864.11 cm-1, which are specific values ​​for glucomannan compounds and using visible spectrophotometry, the glucomannan concentration was obtained at 32.67%.
Co-Authors Abul Hasan Adri Maldi Subardjah Agnes Irene Silitonga Ahmad Baidawi Ahmad Zaki Rasyidi Ali Fikri Hasibuan Ali Mahmudi Alifiana Adhitasari Alparisi, Bima Diokta Amalia, Nindy Putri Amalia, Rezky Martha Amanda, Samira Andri Zainal Apri Setiawan Apriyanto , Indra Aris Suryadi Asmaul Husna Aulia, Rifa AYU WULANDARI Az-Zahra, Tasya Alya Azizah Kholiq B. Wahyudi Joko Santoso Bernadus Joko Santoso Bernadus Wahyudi Bernadus Wahyudi Joko Santoso Budi Triyono Budi Triyono Bunga Muthia Dien Candra Mustika Chilmianida Ainis Syifa Chintia Ni Cinta Rizkia Zahra Lubis Danto Darminto, Citra Dedy Sumardi Dewi, Sagita Tiara Dwi Budi Santoso Ellathodi, Mohammed Razi Emalia Ariska Erzia Yetri Fadhilah Fajar Wahyudi Fatmi, Yulia Fihi Khoirani Sihotang Gunawan Hanris Lukkas Adi Putra Haryati, Nas Heny Lestari Hery Diansyah Putra Houtman Ika Safitri Windiarti Ilham Wahyudi Iqbal Hidayatullah Irwan Jasiah Joko Santoso , Bernadus Wahyu Khairullah Ijlal Khulut Fatimah Kurniawan, Muhammad Ardi Kusumawati, Nyimas Inda Mailatul Maftukhah Maksum Ardi, Hanni Melany Nurjanah Mintarsih Arbarini Mohammad Ridwan Nulloh Muhammad Ihsan Muhammad Sholihul Huda Munir Fahmi, Mokhamad Musnaini Mustofa Mustofa Nas Haryati Setyaningsih Nindy Putri Amalia NOVITA EKASARI Nugraha, Rifa Nur Endah Hikmah Fauziyah Nurdina Safitri Nurhayani Nursahid, Shefira Mahardhika Nuryatin Parwanti, Sri Pertiwi , Vani Mutiara Prasetyo Prasetyo, Dika Rama Prayudani, Santi Raden Sarwani Rasona Pooza Hayati Redi Tian Kustiawan Refson Risfi Aulya Hanni Rita Friyani Rizki Aulia Rohmaniar Rosmeli Samira Amanda Sari, Agelia Sayid Habiburrahman, Sayid Setiani Setianingrum, Reni Dyah Kaeksi setiawan, Dibyo Sitompul, Andreas Jones R Sugianto Supriatini Surismiati Surismiati Suroso Susmiyati Syafira Amelia Syahrul Ghufron Ubaidillah Syakir, Ahmad Syarah Widia Islamiati R Tania Putri Teguh Fachri Fahros Thohiri, Roza Thomas Aquinas J S Tri Joko Raharjo Vetric Styven Silaban Wafiq, Chici Al WAHYUDI Wati, Maulida Laily Kusuma Wenny Novita Sari Yusnida Lase, Yuyun Zada Annuri Nabila Zulham Hidayat