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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Science and Technology Indonesia Jurnal Ilmu Kefarmasian Indonesia Gema Wiralodra Jurnal Kefarmasian AKFARINDO Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Indonesian Journal of Pharmaceutical Education Journal of Social Research Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Ruang Cendekia : Jurnal Pengabdian Kepada Masyarakat Sciences of Pharmacy Jurnal Ilmiah Farmasi Simplisia Journal of Natural Product for Degenerative Diseases
RAHMAT, DENI
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Potential of Secretome Hydrogel for Wound Healing in LPS- and Scratch-Induced BJ Cells as an Inflammation Model Widowati, Wahyu; Rahmat, Deni; Faried, Ahmad; Nainggolan, Ita Margaretha; Priyandoko, Didik; Wargasetia, Teresa Liliana; Sugiaman, Vinna Kurniawati; Triharsiwi, Dwi Nur; Qlintang, Sandy; Murti, Harry; Azis, Rizal; Jeffrey, Jeffrey
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1242-1254

Abstract

Wound healing often requires specialized interventions to accelerate recovery due to prolonged inflammation and limited regenerative factors. Umbilical Cord Mesenchymal Stem Cells secretome (UCMSCs) comprises various cytokines and growth factors that can promote wound healing. This study aims to analyze the potential of a secretome-based hydrogel as a wound-healing agent using BJ fibroblast cells induced by lipopolysaccharide (LPS) and scratch injury as an inflammation model. The secretome hydrogel was formulated using Carbopol, Hydroxypropyl Methylcellulose (HPMC), Hydroxyethyl Cellulose (HEC), and secretome. Cytotoxicity was conducted using the WST-8 assay, while cell migration was evaluated through a scratch assay. Tumor Necrosis Factor-???? (TNF-????), Nuclear Factor kappa-B (NF-????B), and Interleukin-8 (IL-8) gene expression were analyzed via qRT-PCR. Additionally, malondialdehyde (MDA) levels were measured for oxidative stress assessment, whereas Connective Tissue Growth Factor (CTGF) and Transforming Growth Factor-????1 (TGF-????1) levels were quantified using ELISA and colorimetric assays. The secretome hydrogel exhibited no cytotoxic effects on BJ fibroblast cells and significantly enhanced cell migration. Moreover, it reduced the TNF-????, IL-8, and NF-????B expression, indicating anti-inflammatory activity. The hydrogel also decreased MDA levels while increasing TGF-β1 and CTGF expression, suggesting antioxidant properties and enhanced tissue regeneration in the inflammatory model. The secretome-based hydrogel presents a promising therapeutic approach for promoting chronic wound healing by modulating inflammation, reducing oxidative stress, enhancing tissue regeneration, and stimulating fibroblast migration.
Gel Formulation from a Combination of Sidaguri Leaf Extract (Sida rhombofolia L.) and Chinese Petai Leaves (Leucaena leucocephala L) as Inhibitors of Acne-causing Bacteria (Propionibacterium acne and Staphylococcus aureus) Andriana, Andriana; Chaidir, Chaidir; Rahmat, Deni
Journal of Natural Product for Degenerative Diseases Vol. 1 No. 1 (2023): JNPDD September
Publisher : Faculty of Pharmacy Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58511/jnpdd.v1i1.4619

Abstract

Acne vulgaris, a chronic inflammatory disease of the pilosebaceous unit, is the most common skin condition. Propionibacterium acnes and Staphylococcus aureus are two bacteria that can cause infections. Chinese Petai leaves are known to inhibit acne growth. Sidaguri leaves are also thought to have antibacterial activity. The aims of this study were to determine the effect of the combination of the two extracts on inhibiting the growth of acne-causing bacteria, also to test the gel formula to meet physical and chemical parameters as well as stability to temperature and storage, as well as to test acute dermal. irritation to rabbits. Each extract with a concentration of 0.19%; 0.39%; 0.78%; 1.56%; 3.125%; 6.25%; 12.5%; 25%; 50% were incubated and then the inhibition zone was measured, then the minimum inhibition zone concentration of the two extracts was determined to determine the lowest concentration of the extract that still provided antibacterial activity against the tested bacteria using the well-diffusion method. The extract combination is formulated in a gel preparation with Carbopol 940, Propylene glycol, Phenoxyethanol, TEA, and Aquadest as excipients. Test the antibacterial activity of gel preparations using the well method with positive control clindamycin gel. Then evaluation of the formula includes evaluation of physics, chemistry, and microbiology. The results showed that the combination gel had activity against P. acne and Staphylococcus aureus at a concentration of 3.12% : 1.56%. The combined gel preparation of the two extracts also has a synergistic effect in inhibiting acne bacteria. The formula is resistant to temperature and storage, and it can satisfy chemical and physical parameters.
Antioxidant Activity Cultivated of Soursop (Annona muricata L.) Leaves Extract in Jayasari Village, Pangandaran District, West Java Zahara, Indah; Anwar, Effionora; Laksmitawati, Dian Ratih; Rahmat, Deni
Journal of Natural Product for Degenerative Diseases Vol. 1 No. 1 (2023): JNPDD September
Publisher : Faculty of Pharmacy Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58511/jnpdd.v1i1.5317

Abstract

Indonesia is a source of raw materials for tropical medicines that treat various diseases. One of the places to plant soursop leaves in West Java is Jayasari Village, Pangandaran District. Annona muricata Linn (Soursop) leaves is a medicinal plant widely used as an anti-diabetic, anti-inflammatory, insecticide, antimalarial, anticancer, antibacterial, and antioxidant. Soursop leaves have many benefits because they contain phytochemical compounds. This study aims to determine the phytochemical content, total flavonoid content, and antioxidant activity of A. muricata leaves. This research was carried out in several steps, including extraction, evaporation, phytochemical testing, total flavonoid content, and antioxidant activity. Phytochemicals were extracted with 96% ethanol by maceration. Measurement of flavonoids in extracts was determined using standard quercetin and UV-Vis spectrophotometry method. Antioxidant activity was carried out through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical removal test. The results showed that the ethanol extract of soursop leaves contained alkaloids, flavonoids, phenolics, saponins, and tannins. The results of measurements of flavonoids from the ethanol extract of soursop leaves showed that 1 g of the extract contained 8.32 mg of quercetin equivalent. The ethanol extract of soursop leaves has antioxidant activity, as indicated by the scavenging of DPPH radicals with an IC50 of 56.73 ppm.
Preparation, Evaluation, and In Vitro Antiacne Activity of Cymbopogon citratus DC and Cymbopogon nardus (L.) Rendle Essential Oil Microemulsion Datubara, Martaulina Elisabet; Kumala, Shirly; Rahmat, Deni
Journal of Natural Product for Degenerative Diseases Vol. 1 No. 1 (2023): JNPDD September
Publisher : Faculty of Pharmacy Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58511/jnpdd.v1i1.5463

Abstract

This study was conducted to formulate and evaluate a topical anti-acne microemulsion containing essential oils of Cymbopogon citratus DC and Cymbopogon nardus (L). Using the disc diffusion method, the antibacterial activity of the essential oils of C. citratus and C. nardus against Propionibacterium acne and Staphylococcus epidermidis was investigated. After identifying the active concentration, the formula for a topical microemulsion was devised and tested for physical and chemical parameters. The results revealed that the essential oils of C. citratus and C. nardus had antimicrobial activity against P. acnes and S. epidermidis. The results demonstrated that the obtained microemulsion meets the physical and chemical requirements and possesses antimicrobial activity. It can be concluded that the essential oils extracted from C. citratus and C. nardus have the potential for further development and commercial application in the treatment of acne.
EFEK ANTIINFLAMASI NANOPARTIKEL KITOSAN-EKSTRAK KERING TERIPANG (Stichopus variegatus) SECARA IN VITRO DAN IN VIVO Hermawati, Ema; Abdillah, Syamsudin; Rahmat, Deni
Media Farmasi: Jurnal Ilmu Farmasi Vol. 18 No. 2: September 2021
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v18i2.21448

Abstract

Ekstrak teripang mengandung glukosamin yang berpotensi dapat menekan peradangan (inflamasi) dan menyembuhkan osteoarthritis. Penelitian ini bertujuan untuk menguji  aktivitas antiinflamasi secara in vitro dan in vivo dari nanopartikel kitosan-ekstrak kering teripang (Stichopus variegatus). Aktivitas antiinflamasi secara in vitro dilakukan dengan metode denaturasi protein Bovine Serum Albumin (BSA). Aktivitas antiinflamasi secara in vivo dilakukan dengan metode induksi karagenan pada telapak kaki tikus dengan parameter pengukuran edema kaki. Penelitian ini menggunakan tikus putih jantan galur Sprague-Dawley dan dibagi menjadi 4 kelompok yaitu kelompok yang diberi nanopartikel kitosan-ekstrak teripang kering dosis 50 mg/KgBB dan 100 mg/Kg BB, kelompok kontrol negatif (aquadest) serta kelompok kontrol positif (natrium diklofenak). Hasilnya menunjukkan bahwa sampel nanopartikel memiliki aktivitas antiinflamasi secara in vitro dengan nilai IC50 sebesar 267,76 bpj. Nanopartikel kitosan-ekstrak teripang kering dosis 50 mg/Kg BB dan 100 mg/KgBB memiliki aktivitas antiinflamasi dengan nilai persentase penghambatan inflamasi secara berturut-turut sebesar 53,37%, dan 49,95 %. Adapun nilai ED50 yaitu sebesar 99,27 mg/KgBB.
Nanopartikel ekstrak etil asetat daun melinjo (gnetum gnemon l.) dengan aktivitas antioksidan dan antibakteri terhadap propionibacterium acnes Puspitasari, Ranny; Rahmat, Deni; Djamil, Ratna
Gema Wiralodra Vol. 14 No. 1 (2023): Gema Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/gw.v14i1.367

Abstract

Melinjo (Gnetum gnemon L) mengandung asam askorbat, tokoferol, dan polifenol (fenol sederhana, flavonoid, dan tanin. Senyawa kimia ekstrak G. gnemon diperoleh tiga senyawa yaitu 3,4-dimethoxychlorogenic acid, resveratrol, dan 3-methoxyresveratrol. Daun G. Gnemon memiliki potensi antioksidan dan antibakteri. Tujuan penelitian ini membuat ekstrak dan nanopartikel ekstrak daun G, gnemon, serta mengetahui aktivitas antioksidan, antibakteri terhadap bakteri Propionibacterium acnes. Ekstraksi dilakukan dengan metode kinetik dengan pelarut etil asetat. Metode yang digunakan untuk pembuatan nanopartikel yaitu metode gelasi ionik dengan kitosan-Na TPP. Uji aktivitas antioksidan dengan metode DPPH dan uji akstivitas antibakteri menggunakan metode difusi cakram. Hasil menunjukkan ekstrak daun G gnemon memiliki aktivitas antioksidan lebih baik dibandingkan nanopartikel ekstrak daun G gnemon dimana nilai IC50 masing-masing sebesar 35,602 dan 55,105 ppm. Sedangkan untuk aktivitas antibakteri nanopartikel ekstrak G gnemon lebih kuat dibandingkan ekstrak daun G gnemon dimana zona hambat sebesar 12,3 mm. Analisis data dengan uji Mann-Whitney menunjukkan nilai Asymp Sig (2-tailed) < 0,05 terdapat perbedaan yang signifikan. Ukuran partikel Nanopartikel ekstrak daun G. gnemon yaitu (419,4 mm), indeks pendispersi (0,572%) dan potensi zeta (-68,5 9 mV). Ukuran nanopartikel memenuhi syarat karena <1000 mm
Identification and Antibacterial Activity of Propionibacterium Acnes of Ethyl P-Methoxy Cinnamate Isolated from Kaempferia Galanga Linn Utami, Usi Hikmah; Djami, Ratna; Rahmat, Deni; Fajriah, Sofa; Swandiny, Greesty Finotory
Journal of Social Research Vol. 3 No. 1 (2023): Journal of Social Research
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/josr.v3i1.1718

Abstract

Kaempferia galanga Linn is a typical Indonesian plant that contains the compound Ethyl P-Methoxy Sinnamate which has antibacterial activity. Acne is a common skin condition characterized by inflammation of the hair follicles and oil glands in the skin. The most effective acne treatment is the use of antibiotics. This research is an experimental study and aims to identify galangal rhizome isolates (Kaemperia galangan L) obtained from N-Hexane extract. using Thin Layer Chromatography (TLC) and Densitometry TLC as well as testing the antibacterial activity of the acne-causing Propionibacterium acnes ATCC 6919 using Complete Tool Design (RAL) with SPSS analysis, confidence level (p<0.05) and continued with the ANOVA test using the disc diffusion and liquid dilution methods for MIC. On In Vitro test Kaempferia galanga Linn contains antibacterial compounds that cause acne, namely Ethyl P-Methoxy Sinnamate with medium-strong strength at a concentration of 0.6%; 1.2%, and 2.4% with an inhibition zone of 8.70 mm; 10,10 mm and 11.70 mm. The clear zone for Clindamycin is 27.30 mm. Ethyl P-Methoxy Cinnamate (EPMS) isolated from Kaempferia galanga Linn N-Hexane extract Kaempferia galanga Linn has a concentration of 1.572 g/mL and has activity against Propionibacterium acnes bacteria at a concentration of 0.6%; 1.2% and 2.4%
Pemberdayaan kelompok masyarakat Desa Gunung Sari Kecamatan Pamijahan Kabupaten Bogor dalam pemanfaatan tanaman obat keluarga (TOGA) dan pembuatan jamu yang baik Swandiny, Greesty F; Syamsudin, Syamsudin; Putra, Riza Dharma; Rahmat, Deni
Ruang Cendekia : Jurnal Pengabdian Kepada Masyarakat Vol. 1 No. 4 (2022): Ruang Cendekia : Jurnal Pengabdian Kepada Masyarakat
Publisher : ARKA INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55904/ruangcendekia.v1i4.408

Abstract

Tujuan kegiatan PKM membantu kelompok tani untuk dapat mengelola TOGA secara mandiri dengan melakukan pemberdayaan dalam memanfaatkan lahan pekarangan sebagai basis ekonomi lokal masyarakat kemudian tahapan berikutnya adalah upaya pembuatan jamu yang dapat diproduksi sehingga dapat membentuk unit usaha kecil masyarakat. Metode yang digunakan adalah membantu edukasi manfaat TOGA dengan memanfaatkan pekarangan rumah, pembuatan jamu kemudian dapat bermanfaat dan menambah nilai ekonomi masyarakat. Metode pendekatan yang digunakan adalah dengan brainstorming untuk membuka pola piker tentang pentingnya memiliki TOGA, menanamkan jiwa kewirausahaan masyarakat dengan memproduksi jamu yang baik yang tahapan paling penting dapat membentuk produk unggulan masyarakat dengan standar dan perizinan yang berlaku. Target dan capaian Kelompok masyarakat yang merupakan petani dan buruh tani dapat membuat kegiatan bermanfaat dengan mengelola TOGA, menanam tumbuhan berkhasiat obat yang dapat dimanfaatkan secara mandiri oleh masyarakat sekitar dan dapat membuat jamu secara mandiri. Luaran yang dihasilkan a) Luaran wajib : Terbentuknya kelompok tani yang mengelola TOGA yang dapat dikenal sebagai apotek hidup Desa Gunung Sari, terciptanya lahan pekarangan yang dimanfaatkan untuk menanam tanaman berkhasiat obat yang dapat dibuat sebagai bahan utama pembuatan jamu.b) Luaran tambahan : kegiatan diunggah pada youtube, pemberitaan di media massa dan satu artikel ilmiah yang dipublikasikan melalui Jurnal ber ISSN atau prosiding ber ISBN dari seminar nasional.
Aktivitas Antibakteri Kombinasi Ekstrak Daun Jambu Biji (Psidium guajava L.) dan Daun Sambang Darah (Excoecaria cochinchinensis Lour.) terhadap Bakteri Penyebab Jerawat Sinuhaji, Indra; Taurhesia, Shelly; Rahmat, Deni
JURNAL ILMIAH FARMASI SIMPLISIA Vol. 5 No. 2 (2025): Desember 2025
Publisher : Jurusan Farmasi, Politeknik Kesehatan Kementerian Kesehatan Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/jifs.v5i2.912

Abstract

Jerawat merupakan peradangan kulit umum yang sering kambuh dan dapat menurunkan mutu hidup. Infeksi bakteri seperti Cutibacterium acnes dan Staphylococcus aureus merupakan faktor penting dalam patogenesisnya. Tumbuhan berpotensi sebagai sumber antibakteri alamiah karena mengandung metabolit sekunder flavonoid, tanin, saponin, dan alkaloid yang mampu menghambat pertumbuhan bakteri, menurunkan risiko resistensi, dan menjadikannya kandidat terapi antimikroba prospektif. Penelitian ini bertujuan mengevaluasi aktivitas antibakteri kombinasi ekstrak daun jambu biji varietas kristal dan daun sambang darah terhadap bakteri penyebab jerawat (Cutibacterium acnes, Staphylococcus aureus, dan Staphylococcus epidermidis) menggunakan metode sumuran. Proses penelitian meliputi determinasi dan standarisasi simplisia, pembuatan ekstrak, serta pengujian aktivitas antibakteri. Hasil uji mutu menunjukkan simplisia dan ekstrak memenuhi semua standar parameter kimia (senyawa larut, kadar air, kadar abu) dan cemaran mikroba. Uji antibakteri menunjukkan bahwa kombinasi ekstrak pada rasio 1:2 (daun jambu biji : daun sambang darah) menghasilkan zona hambat yang lebih besar dibandingkan ekstrak tunggal pada bakteri Cutibacterium acnes (14,16 mm), Staphylococcus aureus (14,02 mm), dan Staphylococcus epidermidis (12,27 mm). Analisis one-way ANOVA dan post hoc menunjukkan hasil signifikan (p< 0,05), mengindikasikan adanya efek sinergis antar senyawa bioaktif kedua tanaman.
Co-Authors . Syamsudin Ahmad Faried Aji, Nur Akhsani, Luthfia Wikhdatul Aulena, Desi Nadya Azis, Rizal Chaidir Chaidir Datubara, Martaulina Elisabet Dian Ratih Laksmitawati Didik Priyandoko Djami, Ratna Djamil, Ratna Dwi Fitriyani, Dwi Effionora Anwar Elisabeth, Mariska Errol Rakhmad Noordam Esti Mumpuni, Esti Girsang, Ermi Hadiprasetyo, Dhanar Septyawan Harjanti, Mathelda Weni Harry Murti Haryanti, Mathelda Weni Hermawati, Ema Indarwati Indarwati Jeffrey ., Jeffrey Komala, Ismiarni Marisca Evalina Gondokesumo, Marisca Evalina Nainggolan, Ita Margaretha Nancy Dewi Yuliana Nelly Suryani Ni Made Dwi Sandhiutami Nindya, Faradhina Salfa Ofa Suzanti Betha Panca, Pra Panca Bayu Chandra Pra Panca Bayu Chandra Prayoga, Tria Puspitasari, Ranny Putra, Riza Dharma Qlintang, Sandy Raihan, Dany Sabrina Dahlizar Saufa, Zahra Qisthi Shirly Kumala Sinuhaji, Indra Sofa Fajriah Sugiastuti, Fenty Swandiny, Greesty F Swandiny, Greesty Finotory Syamsudin Syamsudin Takasenserang, Oktaviana Taurhesia, Shelly Teresa Liliana Wargasetia Triharsiwi, Dwi Nur Utami, Usi Hikmah Vinna Kurniawati Sugiaman, Vinna Kurniawati WAHYU WIDOWATI Yesi Desmiaty, Yesi Zahara, Indah Zahiroh, Fadhilah Haifa Zaidan, Sarah