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Distribution and Abundance of Actinomycetes in the Rhizosphere Soil of The Hungayono Karst Ecosystem, Gorontalo Apriliyanti Mohulaingo; Yuliana Retnowati; Abubakar Sidik Katili; Ani M. Hasan; Novri Youla Kandowangko
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1363-1371

Abstract

Karst ecosystems have unique environmental conditions that can influence the presence of soil microorganisms, including Actinomycetes in the rhizosphere. This study aimed to determine the distribution and abundance of Actinomycetes in the rhizosphere soil of plants in the Hungayono karst ecosystem, Gorontalo. This study employed a quantitative descriptive method with an exploratory approach. Rhizosphere soil samples were collected from several plant species at three observation points: the lower, middle, and upper parts of the karst area. Actinomycetes were isolated using Starch Casein Agar, while their abundance was determined using the Total Plate Count method and expressed in CFU/g of soil. The results showed that actinomycetes were found at all observation points, with abundances ranging from 2 × 103 to 3 × 105 CFU/g. The highest abundances were found in the rhizosphere of Calamus spp., Anthocephalus macrophyllus, and Ficus microcarpa, while the lowest abundances were found in the rhizosphere of Alocasia macrorrhizos. The Actinomycetes isolates obtained exhibited morphological variation based on mycelium color and colony shape. These findings indicate that the distribution and abundance of Actinomycetes in the rhizosphere soil of the Hungayono karst ecosystem vary among plant species and observation sites. The plant rhizosphere in this karst ecosystem functions as a microhabitat that supports the presence of Actinomycetes and is relevant to studies of karst soil microbiology.
Konseptual Teoritis dan Implementasi Praktis Multimedia Interaktif dalam Pembelajaran Pendidikan Agama Kristen Sahat Patar Lumban Gaol; Hamzah B. Uno; Novri Y Kandowangko; Nina Lamatenggo
Jurnal Kewarganegaraan Vol 6 No 2 (2022): Desember 2022
Publisher : UNIVERSITAS PGRI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31316/jk.v6i2.4016

Abstract

AbstrakSemangat belajar yang tinggi peserta didik berasal dari seorang guru yang mampu menciptakan iklim pembelajaran bermakna, berkualitas dan inovatif, yang mampu menarik perhatian peserta didik. Peserta didik akan terkesan dan termotivasi apabila pembelajaran dikemas melalui media interaktif. Peserta didik sudah lumrah dengan teknologi dan lebih tertarik dengan kemajuan teknologi jaman sekarang. Seorang guru yang berkompeten harus peka dan melihat kondisi perkembangan zaman masa kini, perlu mengevaluasi metode mengajar yang diterapkan. Teknologi sudah marak di dunia pendidikan dimanapun di seluruh dunia. Peran teknologi multimedia mendukung efektifnya pembelajaran dan peserta didik menjadi lebih bersemangat untuk terlibat dalam proses pembelajaran. Teknologi multimedia dimanfaatkan sebagai sarana untuk menciptakan dan mengembangkan pembelajaran. Pengajaran akan lebih menarik perhatian peserta didik, dapat mengoptimalkan hasil belajar yang maksimal. Pelajaran akan lebih jelas maknanya sehingga mudah dipahami. Dan peserta didik menguasai tujuan dari pembelajaran. Metode mengajar guru akan lebih bervariasi dan peserta didik dapat mengamati, menikmati, dan mengembangkan hasil dari informasi yang didapat. Peserta didik, mendapat pengalaman belajar yang berkesan. Informasi yang diterima dapat diingat lebih lama jika seorang guru mampu dengan tepat dalam pemanfaatan teknologi khususnya multimedia interaktif dalam pembelajaran pendidikan agama kristen.Kata Kunci: Multimedia, Pendidikan Agama Kristen
Pemberdayaan Masyarakat Desa Bihe, Kabupaten Gorontalo melalui Diversifikasi Kelapa Menjadi Virgin Coconut Oil dan Cocopeat Novri Youla Kandowangko; Muchtar Ahmad; Mustamin Ibrahim; Febriyanti
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 2 (2024): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.10.2.175-182

Abstract

Bihe Village is one of the villages in Asparaga District, Gorontalo Regency, which is a center for coconut production. The abundant potential of coconut plants has yet to be utilized optimally by the people of Bihe village. Coconuts are only produced as copra, or coconuts are sold whole, without processing. Coconut plants have many derivative products, such as virgin coconut oil (VCO) and coconut peat. This problem prompted the implementation of socialization, training, and assistance in making VCO and Cocopeat for the Bihe village community. The aim is to increase the community's insight and skills regarding the diversification of coconut products so that they can provide additional income, which can improve the economy in Bihe Village. The training method is the Participatory Action Research (PRA) method, which involves the active role of the Bihe village community. The stages carried out are analysis of the actual conditions of society, planning, action, observation, and reflection. This method is carried out to provide an understanding of a) the weaknesses they have, b) the community's desire to overcome their shortcomings and weaknesses, c) together with the community to develop strategies and methods to solve the problem, and d) help the community overcome, solve, and find a way out. Forty participants attended this training and mentoring. After implementing the empowerment program, the post-test results showed that 62.5% of the community gave an excellent assessment, and 37.5% gave a good assessment of the usefulness of this activity program. Furthermore, 72.5% of participants were motivated to open a new coconut commodity business.
Identification Morphology of Bamboo, and Traditional Use in Gorontalo Febriyanti Febriyanti; Novri Youla Kandowangko; Jusna Ahmad
Jurnal Biodjati Vol 8 No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.29714

Abstract

Bamboo is a versatile plant that has many benefits in the lives of the people of Gorontalo. This research aims to provide taxonomic information on bamboo species in Gorontalo Province, including variations in morphological characters and phenetic relationships of bamboo, as well as information on the traditional use of bamboo. The method used in this research is exploration and interviews. Exploration was carried out to collect information on bamboo taxonomy, while interviews were conducted to find out the use of bamboo by the Gorontalo Community. The research data obtained was then analyzed descriptively to describe and interpret data on morphological variations, phenetic relationships and traditional benefits of the various types of bamboo obtained. Based on the research results, it was obtained that 13 types of bamboo are distributed in Gorontalo which show the unique morphological variations of the varieties which are divided into four genera, namely the genus Gigantochloa has distinctive morphological characteristics on the waxy surface of the stem, the Bambusa genus with characteristic shiny, smooth stems, the Dendrocalamus genus has the largest diameter and stem wall thickness, the genus Schizostachyum has the same morphological characteristics of stem segments and branch lengths. Based on the analysis of phenetic relationships, two main clusters were formed with a similarity value of 61.2%. In terms of traditional uses, it was recorded in this research that most bamboo is used as building construction materials, food sources, traditional medicine, materials for traditional ceremonies, handicrafts, and ornamental plants, as a tool designedto help people's livelihoods and ecological functions. This research also provides the first record of the morphological characteristics and use of each type of bamboo in Gorontalo which can be used for future bamboo breeding and conservation projects.
Induksi Kalus dari Antera Pisang Goroho (Musa acuminata L.) dengan Menggunakan 2,4-D dan BAP Risna A. Onu; Jusna Ahmad; Febriyanti Febriyanti; Novri Youla Kandowangko; Devi Bunga Pagalla; Indriati Husain
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/8t6ph238

Abstract

Callus induction is a critical stage in anther culture for supporting the propagation and genetic improvement of local banana cultivars. This study evaluated the effect of different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) on callus induction from goroho banana (Musa acuminata L.) anthers. Observations were conducted for 30 days after induction, focusing on callus emergence time and morphological characteristics, including color and texture. The results demonstrated distinct responses among treatments. The combination of 1.5 ml 2,4-D and 1.5 ml BAP produced the fastest callus initiation with an average of 13.17 days after induction and generated friable callus with a pale yellow coloration, indicating high cellular activity. Lower growth regulator concentrations delayed callus formation, while higher concentrations promoted color changes associated with early tissue differentiation. No callus formation was observed in the absence of exogenous growth regulators. These findings highlight the importance of balanced auxin and cytokinin concentrations in regulating anther-derived callus induction and provide a physiological basis for further development of goroho banana anther culture systems.
Short Communication: Inventory of traditional medicinal plants and their uses from Atinggola, North Gorontalo District, Gorontalo Province, Indonesia NOVRI YOULA KANDOWANGKO; MUKHLISULFATIH LATIEF; RAMPI YUSUF
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190637

Abstract

Kandowangko NY, Latief M, Yusuf R. 2018. Inventory of traditional medicinal plants and their uses from Atinggola, North Gorontalo District, Gorontalo Province, Indonesia. Biodiversitas 19: 2294-2301. Medicinal plants have been used by the people of Gorontalo as a hereditary tradition. But this knowledge has not spread to the wider community because the traditional wisdom about medicinal plants has not been documented, stored and managed properly by employing digital tools. The purpose of this study is to prepare an inventory of the traditional medicinal plants and the details of their uses in Atinggola, North Gorontalo district, Indonesia. Data has been collected by ethnobotanical survey method and analyzed using the descriptive qualitative method. The study has shown that 38 species of medicinal plants, belonging to 20 families, are used to cure many diseases by the traditional healers of Atinggola. Among them, 6 species are used to treat fever, 5 species to treat skin diseases, 2 species each to treat cancer, gastrointestinal diseases, liver diseases, and as body tonic to restore power; 1 species each to treat toothache, malaria, tonsillitis, allergies, eye irritation, wound infections and tuberculosis (TBC). Plant parts used in the treatment practices are leaf, fruit, flower, rhizome, root, stem, seed, shoots, midribs parts, etc. However, the most dominant part used is the leaf of the plants. Various methods such as boiling, squeezing, scraping, chewing, smashing, brewing, etc. are used to prepare the medicines. 29 species (76.31%) of medicinal plants are collected from cultivated sources such as backyards and gardens while 9 species (23.68%) are still sourced from forests.
Traditional agro-management practices, utilization and nutritional composition of momala: A local maize variety of Gorontalo, Indonesia NOVRI YOULA KANDOWANGKO; MARGARETHA SOLANG; ERNA RETNAWATY
Biodiversitas Journal of Biological Diversity Vol. 21 No. 3 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210301

Abstract

Abstract. Kandowangko NY, Solang M, Retnawaty E. 2020. Traditional agro-management practices, utilization and nutritional composition of Momola-a local maize variety of Gorontalo, Indonesia. Biodiversitas 21: 853-859. Maize (Zea mays, L.) or corn, locally called milu or binthe (Gorontalo language), has been a popular crop among Gorontalo people for a long time. People mostly use baby corn as the main ingredient of binthe biluhuta, famous traditional food in Gorontalo. momala is a local maize variety of Gorontalo the cultivation of which is decreasing. The cause is a shift in farmers’ preference of the crop, most of them preferring hybrid variety over momala. Decrease in cultivating local maize has also led to a decline in the local farmers’ traditional knowledge of the crop. Therefore, initiatives to raise awareness of the importance of cultivating local crops are essential for their popularization and conservation purposes. This research is aimed at (i) exploring the traditional knowledge of local farmers pertaining to agro-management of maize or corn, (ii) describing the utilization of momala, and (iii) investigating the nutritional composition of momala, both by qualitative and quantitative methods The results revealed that the corn farmers apply the principle of huyula or gotong royong (communal work) in their agriculture activities. The people are using momala corn as the main ingredient of local food preparations, such as binthe biluhuta (clear corn soup), balobinthe (corn rice), and kokole (soft, pudding-like corn cake), in addition to some traditional rituals. Nutritional analysis indicated that its ash content is 1.34-2.86%, crude protein is 9.09-11.67% crude fat is 4.29-4.96%, and carbohydrate is 67.68-68.16%. Furthermore, the composition of nitrogen-free extract ranges from 57.85-72.96% and metabolic energy content ranges from 2896.94-3352.77 Kcal/kg. Measures to conserve and improve momala are necessary to promote food security of people.
Morphological characteristics and nutritional value of binthe kiki, a local maize variety from Gorontalo, Indonesia Jusna Ahmad; NOVRI YOULA KANDOWANGKO; MARGARETHA SOLANG; ERWIN NADJAMUDIN
Biodiversitas Journal of Biological Diversity Vol. 22 No. 8 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220852

Abstract

Abstract. Ahmad J, Kandowangko NY, Solang M, Najamuddin E. 2021. Morphological characteristics and nutritional value of binthe kiki, a local maize variety from Gorontalo, Indonesia. Biodiversitas 22: 3523-3529. Gorontalo is among the coastal areas in Tomini bay with diverse varieties of local maize. Despite that, the population of local maize has seen a steady decline over the years. A literature study reports that local maize has adapted well to the environment in which it grows. Local maize is also hailed by the community as a source of food, animal feed, and the main ingredient of traditional medicines. The present study aims to investigate the morphological characteristics and nutritional value of binthe kiki local maize variety. Employing a quantitative descriptive method, the data of morphological characteristics were collected by referring to the maize descriptor guidelines, while the data of the nutritional value of maize kernels (regarding the proximate and mineral contents) were analyzed by referring to the Indonesian National Standard and Association of Official Agricultural Chemists (AOAC) standards. The findings show that the morphological characteristics of binthe kiki variety are as follows: (i) an average plant height of 161 cm; (ii) each stalk has three cobs with an average height of 119 cm; (iii) an average stalk circumference of 6.35 cm; (iv) an average of 12 leaves per stalk; (v) 71.12 cm of an average length of leaves; (vi) 5.21 cm of an average width of leaves; (vii) the direction of the leaf blade, on average, is slightly curved; (viii) the average leaf tip is pointed; (ix) an average cob length of 8.4 cm; (x) an average cob diameter of 2.74 cm; (xi) an average cob weight (without husk) of 0.054 kg/cob; (xii) an average of 24 kernels per row; (xiii) an average weight per 1000 kernels of 145 gram. Binthe kiki variety has high contents of protein, carbohydrate, zinc, and calcium, yet it is low in fat contents compared to other varieties.
Genetic diversity of the genus Citrus in Tomini Bay coastal areas, Indonesia based on morphological characters NOVRI YOULA KANDOWANGKO; FEBRIYANTI FEBRIYANTI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 5 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240549

Abstract

Abstract. Kandowangko NY, Febriyanti. 2023. Genetic diversity of the genus Citrus in Tomini Bay coastal areas, Indonesia based on morphological characters. BIodiversitas 24: 2938-2952. Citrus plants are one of the fruit plants that are widely consumed by the community, both for food, health, and customs. The existence of Citrus plants in the coastal area of Tomini Bay only grows wild and has not been cultivated intensively. Lack of information on the diversity of citrus and the use of Citrus plants that are not balanced with cultivation actions will threaten the existence of these Citrus plants. This research aims to provide information about the morphological characteristics of the genus Citrus in the coastal area of Tomini Bay. The plant materials used were 39 accessions consisting of 7 species. Descriptive methods and diversity index calculations as well as analysis of phenetic consanguinity data were applied in this study. The results showed that all Citrus accessions have morphological diversity ranging from stems, leaves, flowers, fruits, and seeds that are influenced by genetic and environmental factors. The diversity index (H’) was equal to 0.8103 which was categorized as low. The 39 Citrus accessions were divided into two main clusters with a similarity index of 64.3%. The findings of this study are expected to be useful in Citrus breeding and conservation programs in the future.
Diversity of actinomycetes on plant rhizosphere of karst ecosystem of Gorontalo, Indonesia YULIANA RETNOWATI; NOVRI YOULA KANDOWANGKO; ABUBAKAR SIDIK KATILI; WAWAN PEMBENGO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 3 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250301

Abstract

Abstract. Retnowati Y, Kandowangko NY, Katili AS, Pembengo W. 2024. Diversity of actinomycetes on plant rhizosphere of karst ecosystem of Gorontalo, Indonesia. Biodiversitas 25: 907-915. The objective of this study was to isolate and identify the actinomycetes associated with the rhizosphere of plants in the karst ecosystem. This study was descriptive-quantitative. Soil sampling was conducted at three locations of the karst ecosystem of Gorontalo, i.e., Bangga Hills at Bangga Bubaa Coastal area of Gorontalo District, Hills around Lake Limboto of Gorontalo District, and Oluhuta Beach Hills at Bone Bolango District. Rhizosphere-soil sample was collected from 15-30 cm of soil depth. Isolation of actinomycetes was performed using the plate method. The screening of phosphate-solubilizing bacteria was based on the ability to solubilize phosphates indicated by the clear zone on the Pikovskaya medium. Identification of actinomycetes bacteria based on morphology and molecular characters. The morphology character included the color of aerial and substrate mycelium. Molecular characteristics based on the sequence of 16S rRNA gene compared to sequence data on the gene bank of NCBI. The phylogenetic tree reconstruction was based on the Neighbor-joining algorithm. The results successfully found six isolates, including three actinomycetes isolates, two non-actinomycetes bacteria, and one yeast. They were isolated from the rhizosphere of Malastoma malabathrum, Chromolaena odorata, Cycas rumphii, Leucaena leucocephala, and Jatropha curcas. The actinomycetes isolate showed plant-growth-promoting potential indicated by solubilizing phosphate and Indole 3-Acetic Acid production activities. Based on the molecular analysis, actinomycetes were closely related to Streptomyces aegyptia (MT505707.1:42-1426), Streptomyces sp. GGCR-6 (MH718844.1:5-1391), and Streptomyces carpaticus strain PES-A23 (MH712039).
Co-Authors Abubakar Sidik Katili Adelia Salsabila Rahman Adila Nindhaulhasanah Adju, Fitria Y.Hs. Ahaya, Cindi Ahmad Hidayat Ahmad, Aisyah Aisyah Ahmad Aisyah Ahmad Akbar, Muh Nur Alfandi Kibu Ali, Fathur Rahman M. Andi Pratiwi Andi Pratiwi Ani Hasan, Ani Ani M. Hasan Apriliyanti Mohulaingo Aryati Abdul Bakari, Iswanto Bambang Mamangkay Bulowe, Yuniarty R Chairunnisa Lamangantjo Citra Leonita Matalauni Cono, Titi Hawanda Metania Dali, Ira K. Dama, Dewi Rahayu Damopolii, Insar Danial Mohamad Devi Bunga Pagalla Devi Bunga Pagalla Dewi Wahyuni K. Baderan Djafar, Fitri Yani Djuna Lamondo Dwinanto, Arif Elawati, Elawati Elsi Gogou Elya Nusantari ERNA RETNAWATY ERWIN NADJAMUDIN Fadlia Dunggio Fathur Rahman M. Ali Fatmariani Abdul Wahab Febriyanti Febriyanti febriyanti febriyanti Febriyanti Febriyanti Frida Maryati Yusuf GIAT SURYATMANA Gintulangi, Sabriana Oktaviana Gogou, Elsi Hamzah B Uno Hartono D. Mamu Hayatiningsih Gubali Husain, Holiyana Husain, Ilyas H. Ilyas Husain Indayani Tandililing Indiriati Husain Indri Meliyani Bouta Indriati Husain Ishak, Rafli Isima , Lindawaty Iswanto Bakari Jan Hendriek Nunaki Jusna Ahmad Kadita P. Latif Keley, Umar Keley, Umar Labuga, Falerins ladoma, wafiq aziza m. Lamatenggo, Nina Lindawaty Isima Lindawaty Isima Magfirahtul Jannah Margaretha Solang Marini Susanti Hamidun Masra Latjompoh Mega Rosalita Ekaputri Koni Miftahul F. Adudu Miftahul Fikriyah Adudu Muchtar Ahmad Muh. Nur Akbar Muhammad Isra Mukhlisulfatih Latief Mustamin Ibrahim Mustaqimah, Nur Mutmainah Mutmainah Nazifah R Adam Nazwa S. Lamante NENNY NURLAENY Nurdin . Nuryanti Rumalolas Pagala, Devi Bunga Pakaya, Melisa Patta Sija Potutu, Bayu S. Putri, Tarissa Eka Rahman, Iyam Rahwilin Van Gobel Ramli Utina Rampi Yusuf Rianjani, Devi Tutut Rianjani, Devi Tutut Rinna Amelia Polihito Risna A. Onu Rizal Suleman Rizaldi Mokodompit ROBERT DJONGGI MARULI SIMANUNGKALIT Rosely, Meilin S. Y. Sahat Patar Lumban Gaol Saifullah Mamonto Suwarni Karim Syafar, Sitti Zaenab Tadidik, Ulfaida H. Timbola, Muh Kirad Ui, Maryam Walangadi, Farhana Rahmatia Wawan Pembengo Widyawati S. Isima Wilinda A. Datau Winda Jakaria Wirnangsi Din Uno Wirnangsih D. Uno Yelsi Patiung Yuliana Nurdian Toini Yuliana Retnowati Yumna Binti Abud Alamri Yuniyarti Hulu Yusrin Anton Puluhulawa Yusriyah Yusriyah Zuliyanto Zakaria Zusriadi, Brenda Febrina