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All Journal Journal Of Vocational Health Studies Klinikal Sains (Jurnal Analis Kesehatan) Care : Jurnal Ilmiah Ilmu Kesehatan Jurnal Riset Kesehatan Poltekkes Depkes Bandung INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Ners Jurnal teknologi Kesehatan Borneo MAHESA : Malahayati Health Student Journal Jurnal EduHealth JURNAL ILMIAH GLOBAL EDUCATION Innovative: Journal Of Social Science Research Jurnal Vokasi Kesehatan Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Media Penelitian dan Pengembangan Kesehatan MEDICA (International Medical Scientific Journal) Jurnal Info Kesehatan ELECTRON (Journal of Science and Technology)
Ari Nuswantoro
Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
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Potential of Garlic Filtrate as An Alternative Anticoagulant for Whole Blood Samples Ari Nuswantoro; Jessica Ningtyas Berlianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2683

Abstract

Synthetic anticoagulants such as heparin, citric, and ethylenediaminetetraacetic acid (EDTA) are commonly used to prevent blood clots. In contrast, its widespread use in clinical laboratories is still constrained by price, its toxic nature, and its short shelf life. Therefore, an alternative material that is relatively cheap, non-toxic, and easy to obtain and process in a ready-to-use form is needed. Garlic contains allicin and ajoene, which are anti-platelet and anti-thrombogenic. This study’s aim is to explore the potential of garlic filtrate as an alternative anticoagulant.  Blood from 16 individuals was used and separated into four groups: non-anticoagulant, 50 μL/mL garlic filtrate, 100 μL/mL garlic filtrate, and 150 μL/mL heparin, for a total of 64 treatments. The Lee and White method showed that non-anticoagulated blood had normal clotting times (mean 8 minutes and 56 seconds), whereas heparin plasma and garlic filtrate plasma had longer clotting times (more than 20 minutes); and this is statistically different based on the ANOVA test with a significance value (p) of 0.000 < 0.05. On spectrophotometric measurements, the levels of calcium ions in heparinized plasma and serum were 8.66 mg/dL and 8.52 mg/dL, respectively, while in garlic plasma filtrate of 50 μL/mL and 100 μL/mL were 4.13 mg/dL and 3.58 mg/dL, respectively; this is also statistically different based on the ANOVA test with a significance value of 0.000 < 0.05. The differences indicate that garlic filtrate can extend clotting time and reduce calcium ions therefore it is worth reviewing as an alternative anticoagulant.
Combination of Aloe Vera and Chocolate to Reduce The Number of The Colony of Staphylococcus Aureus Edy Suwandi; Ari Nuswantoro; Sugito Sugito
Jurnal teknologi Kesehatan Borneo Vol 3 No 2 (2022): Jurnal teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v3i2.197

Abstract

Aloe vera (AV) and chocolate are good examples of functional foods because they contain prebiotics that can help the host defend itself against harmful bacterial infections such as Staphylococcus aureus. Many studies have used AV or chocolate alone to help fight infection, but not much is known about using both together. This study aims to count the number of colonies of S. aureus infected on mice’s skin (Mus musculus) after being given AV and cocoa in certain doses per kilogram of body weight. A total of 35 healthy male mice were infected with S. aureus in the superficial area of ​​the nape of the skin. After that, they were divided into seven groups, each consisting of 5 mice, and given the following treatments: given 50 mg/kg BW AV (group A), 50 mg/kg BW brown (B), 50 mg/kg BW combination of AV and chocolate (C), given 75 mg/kg BW AV (D), given 75 mg/kg BW brown (E), given 75 mg/kg BW combination of AV and chocolate (F), and control (without prebiotic administration), K). Mice that were given 75 mg/kg BW AV/chocolate/AV+chocolate had a better ability to reduce the number of bacteria than those that were given 50 mg/kg BW AV/chocolate/AV+chocolate. In addition, rats that were given AV and chocolate together had a better ability to reduce the number of bacteria than those that were given either only AV or chocolate alone. Prebiotics are both found in AV and chocolate but with different substances. This causes mice given a combination of prebiotics to be considered better than those given only one type of prebiotic source.
Pengaruh Penambahan Lidah Buaya (Aloe barbadensis Miller) pada Minyak Goreng Bekas terhadap Kadar Bilangan Asam Maulidiyah Salim; Gervacia Jenny Ratnawati; Ari Nuswantoro
Jurnal Vokasi Kesehatan Vol 7, No 1 (2021): JANUARI 2021
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.639 KB) | DOI: 10.30602/jvk.v7i1.833

Abstract

Minyak goreng bekas memiliki kadar bilangan asam yang tinggi dan dapat menyebabkan kerusakan minyak tersebut. Tujuan dari penelitian ini adalah untuk mengetahui apakah ada pengaruh penambahan lidah buaya (Aloe barbadensis Miller) pada minyak goreng bekas terhadap kadar bilangan asam. Desain penelitian yang digunakan adalah eksperimen semu, menggunakan Teknik purposive sampling. Sampel adalah minyak goreng yang telah mengalami proses penggorengan secara berulang yang dibagi menjadi ke dalam 4 kelompok perlakuan dan masing-masing 6 pengulangan sehingga total sampel menjadi 24. Kadar asam lemak bebas diperiksa dengan menggunakan metode alkalimetri. Nilai rata-rata kadar bilangan asam pada kelompok yang terdiri dari tanpa penambahan lidah buaya, dengan penambahan lidah buaya 25 gram, dengan penambahan lidah buaya 50 gram, dan dengan penambahan lidah buaya 75 gram berturut-turut adalah 0,17%, 0,15%, 0,11%, dan 0,09%.  Hasil uji regresi linier sederhana memberikan nilai probabilitas 0,000 (p < 0,05) sehingga dinyatakan ada pengaruh penambahan lidah buaya pada minyak goreng bekas terhadap kadar bilangan asam.
Lipid Profile of Prolanis Patients in Pontianak City Ari Nuswantoro; Dinasti Aprillia; Juliana Cristyaningsih
Jurnal teknologi Kesehatan Borneo Vol 4 No 1 (2023): Jurnal teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v4i1.209

Abstract

Perubahan lingkungan, teknologi dan gaya hidup memicu naiknya kasus penyakit tidak menular, seperti diabetes melitus (DM) dan hipertensi (HT), yang bersifat kronis dan membawa risiko kematian terutama di negara berkembang seperti Indonesia. Dua penyakit tersebut dapat dideteksi dengan menilai profil lipid individu dimana kondisi dislipidemia dapat dianggap mengarah pada risiko terjadinya penyakit kardiovaskuler. Pemerintah Indonesia meluncurkan program Prolanis terhadap pasien DM dan HT yang salah satu kegiatannya adalah pemeriksaan profil lipid yang meliputi kadar kolesterol, trigliserida, HDL-kolesterol, dan LDL-kolesterol. Dari hasil pemeriksaan ini dapat dinilai rasio trigliserida terhadap HDL-kolesterol, yang merupakan prediktor paling kuat terhadap penyakit kardiovaskuler. Penelitian ini mengambil data pemeriksaan profil lipid dari 337 pasien Prolanis di Kota Pontianak. Hasil penelitian menunjukkan bahwa pasien DM didominasi oleh orang dengan hiperkolesterolemia (63,03%), trigliserida normal (56,36%), HDL-kolesterol normal (91,51%), LDL-kolesterol tinggi (83,03%), dan rasio trigliserida/HDL-kolesterol normal (60%). Demikian pula pada pasien HT, hiperkolesterolemia dan LDL-kolesterol tinggi dialami sebagian besar pasien (69,76 dan 84,30%), sedangkan trigliserida normal, HDL-kolesterol normal, dan rasio Trigliserida/HDL-kolesterol normal lebih mendominasi dengan persentase 61,61%, 94,19%, dan 68,03%. Hasil penelitian ini menunjukkan bahwa baik pasien DM maupun HT memiliki kecenderungan profil lipid yang normal dan menuju dislipidemia.
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN KITOLOD (Isotoma longiflora) TERHADAP PERTUMBUHAN BAKTERI Streptococcus pyogenes Ari Nuswantoro; Kartini Kartini
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3343

Abstract

Kitolod is a plant that is easy to grow in the tropics, including Indonesia. Kitolod leaves contain flavonoids, alkaloids, tannins, saponins and terpenoids that have the potential to be used as antibacterial agents to inhibit the growth of pathogenic bacteria such as Streptococcus pyogenes. This study aims to determine the antibacterial activity of kitolod leaf extract on the growth of Streptococcus pyogenes. This research is quasi-experimental with purposive sampling technique. Kitolod leaves were extracted by maceration method using ethanol then dissolved in 15% DMSO to obtain concentrations of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, and 90%. Each concentration was replicated three times, and the control used the antibiotic bacitracin. After carrying out the antimicrobial sensitivity test using the Kirby-Bauer diffusion method, the results obtained were inhibition zones of 17.66 mm (sensitive), 18.16 mm (sensitive), 18.50 mm (sensitive), 19 mm (sensitive), 19. 83 mm (sensitive), 21.16 mm (sensitive), 21.83 mm (sensitive), 22.33 mm (sensitive), and 23.83 mm (sensitive) respectively, while the bacitracin control was 17.5 mm (sensitive). Thus, kitolod leaf extract proved to have antibacterial activity against the growth of Streptococcus pyogenes.
Identifikasi Bakteri Coliform pada Minuman Air Tahu dan Air Tebu yang Dijual di Wilayah Kota Pontianak Ramadan, Aditiya; Jordan, Aristo; Ardhani, Adha Riesta; Monalita, Ria; Munardi, Fhatia Natasya; Syahdilla, Ade; Nuswantoro, Ari; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1470

Abstract

Air tahu dan air tebu merupakan minuman manis dan menyegarakan yang cukup diminati oleh masyarakat Kota Pontianak terutama dari kalangan anak-anak, remaja, dan orang tua. Cemaran bakteri Coliform yang terdapat pada minuman air tahu dan air tebu yang dijual di Kota Pontianak dapat menyebabkan masalah serius pada kesehatan. Tujuan dari penelitian ini adalah untuk mengetahui adanya cemaran bakteri Coliform pada minuman air tahu dan air tebu yang dijual di pinggir jalan Kota Pontianak dengan menggunakan metode kultur. Desain penelitian ini adalah penelitian deskriptif dengan pengambilan sampel secara total sampling. Penelitian ini dilakukan dengan cara mengambil sebanyak 43 sampel air tahu dan 70 sampel air tebu yang dijual di pinggir jalan di Kota Pontianak. Hasil penelitian dari 43 sampel air tahu menunjukkan 17 sampel (39,5%) positif bakteri Coliform, 26 sampel lainnya (60,5%) negatif bakteri Coliform, dan hasil peneilitan dari 70 sampel air tebu menunjukkan 37 sampel (52,8%) positif bakteri Coliform, dan 33 sampel lainnya (47,2%) negatif Coliform.
PROFILE OF RENAL FUNCTION LABORATORY EXAMINATIONS IN PROLANIS PATIENTS IN PONTIANAK CITY Nuswantoro, Ari; Aprillia, Dinasti; Purwaningsih, Indah; Arifah, Rizki Zanuba
Jurnal Laboratorium Khatulistiwa Vol 5, No 2 (2022): Mei 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v5i2.979

Abstract

Diabetes mellitus (DM) and hypertension is one of the chronic diseases that cause health problems in Indonesia. The government organizes a Management Program Chronic Disease (Prolanis) in order to manage patients with both of these diseases. One of the activities carried out is laboratory tests of urea and serum creatinine. This study aims to describe the number of DM and hypertension sufferers as well as serum urea and creatinine levels in Prolanis participants which checked themselves into the Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak. The study was conducted using a total sampling method by looking at the medical record data of Prolanis participants, who will check themselves to the Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak, from January – December 2021 so that data were obtained from 333 patients consisting of 84 men and 349 women with an age range of 26 – 91 years. The results showed that 163 patients were diagnosed with DM and the remaining 170 people suffered from hypertension. The average value of urea levels to male patients is 36.4 mg/dL (14 – 113 mg/dL) and to women it is 27.3 mg/dL (11 – 80 mg/dL). The average value of creatinine levels to men is 1.3 mg/dL (0.5 – 5.3 mg/dL) and to women is 0.8 mg/dL (0.4 – 1.9 mg/dL). Urea and creatinine levels are theoretically associated with the incidence of DM and hypertension.
Pengukuran Nilai MPN Pada Air Minum Isi Ulang Di Wilayah Kota Pontianak Yang Disterilisasi Dengan Portabel Ultraviolet Dan Portabel Filtrasi Reza, Nur; Nuswantoro, Ari; Indrawati, Ratih; Muhammad Ihsan, Bagus
Innovative: Journal Of Social Science Research Vol. 3 No. 6 (2023): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Air merupakan kebutuhan utama manusia untuk bertahan hidup sedangkan sumber air bersih alami semakin banyak yang tercemar. Sebagai alternatif, masyarakat mendapatkan air minum dari depot air minum isi ulang (DAMIU) yang menggunakan metode sterilisasi portabel ultraviolet (UV) dan portabel filtrasi. Namun demikian, bakteri coliform masih ditemukan pada air minum karena air baku yang tercemar, alat yang digunakan tidak steril, dan minimnya wawasan masyarakat mengenai sanitasi dan higien DAMIU. Tujuan penelitian ini menghitung nilai most probable number (MPN) pada DAMIU di Kota Pontianak. Penelitian ini berbentuk deskriptif eksploratif berdesain cross-sectional. Data diperoleh dengan melakukan pemeriksaan MPN terhadap 36 sampel DAMIU. Hasil menunjukkan 14 (77,78%) DAMIU metode portabel ultraviolet dan 17 DAMIU (94,44%) portabel filtrasi menunjukkan nilai MPN di atas batas yang ditentukan. Banyaknya DAMIU yang tidak sesuai persyaratan kualitas air minum layak konsumsi sesuai Permenkes menunjukkan adanya risiko penularan penyakit yang disebabkan oleh coliform melalui air minum DAMIU di Kota Pontianak.
POTENSI TEPUNG KULIT PISANG RAJA SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Penicillium sp. Kamilla, Laila; Fadilla, Alda Nur; Nuswantoro, Ari; Tumpuk, Sri
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1665

Abstract

Sumber karbohidrat yang mudah ditemukan dan belum banyak dimanfaatkan adalah tepung kulit pisang raja. Kulit pisang raja memiliki potensi untuk digunakan sebagai media alternatif karena memiliki kandungan zat seperti karbohidrat, protein, dan lemak. Tujuan penelitian ini untuk mengetahui potensi tepung kulit pisang raja sebagai media pertumbuhan jamur Penicillium sp. Penelitian ini menggunakan desain penelitian Quasi Experiment. Populasi dalam penelitian ini yaitu kulit pisang raja. Sampel yang digunakan adalah tepung kulit pisang raja yang dibuat dalam konsentrasi 10%, 20%, 30%, 40%, 50% dengan 5 kali pengulangan didapatkan jumlah sampel 25. Hasil penelitian yaitu konsentrasi 10% rata- rata 11,6 CFU, konsentrasi 20% rata-rata 12,8 CFU, konsentrasi 30% rata-rata 17,4 CFU, konsentrasi 40% rata-rata 21,8 CFU, konsentrasi 50% rata-rata 28 CFU dan media standar Sabouraud Dextrose Agar (SDA) koloni diperoleh rata-rata 152,6 CFU. Hasil uji Anova didapatkan nilai 0,000 < 0,05 yang menyatakan ada perbedaan pertumbuhan jumlah koloni pada tiap konsentrasi media alternatif dengan media standar Sabouraud Dextrose Agar (SDA) dapat disimpulkan media alternatif tepung kulit pisang raja berpotensi digunakan sebagai media pertumbuhan jamur Penicillium sp. namun tidak dapat menggantikan media standar Sabouraud Dextrose Agar (SDA).
PERBEDAAN HASIL HITUNG BERBAGAI JENIS LEUKOSIT PADA PASIEN COVID-19 YANG ISOLASI MANDIRI DENGAN ISOLASI DI RSUD KABUPATEN MELAWI Reza, Nur; Santoso, Edi; Nuswantoro, Ari; Indrawati, Ratih; Nurhayati, Etiek; Aditia, Aditia
Media Penelitian dan Pengembangan Kesehatan Vol. 32 No. 3 (2022): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v32i3.1886

Abstract

COVID-19 infection causes various clinical manifestations so the treatment method is also different for each individual. Patient isolation is one of the treatments to break the chain of transmission of COVID-19. Asymptomatic to moderate symptomatic patients are usually asked to self-isolate, while those with severe and critical symptoms are isolated in hospital. Leukocyte examination is an important parameter and plays a role in determining patient follow-up. The aim of this study was to find out whether there were differences in leukocyte differential-counts in COVID-19 patients who were self-isolating and those isolated at Melawi Regional Hospital. This study had a cross-sectional design by taking data from medical records of COVID-19 patients at Melawi Regional Hospital during October 2021 to July 2022. The data taken were from 399 COVID-19 patients, consisting of 182 patients are self-isolating and 217 patients are isolating at Melawi Regional Hospital. In self-isolated patients, the average value of leukocyte count was 7.04 x 10³ cells/µL, and the leukocyte differential-counts was neutrophils 58.45%, lymphocytes 21.81%, monocytes 13.84%, eosinophils 1.52%, and basophils 4.38%. In isolated patients at Melawi Regional Hospital, the average value of leukocyte count was 9.89 x 10³ cells/µL, and the leukocyte differential-counts was neutrophils 75.09%, lymphocytes 12.97%, monocytes 8.38%, eosinophils 0.46%, and basophils 3.11%. The Mann-Whitney U test obtained a significance value of 0.000 (p < 0.05), which means there is a significant difference in the results of leukocyte counts between self-isolated patients and those isolated at Melawi Regional Hospital.
Co-Authors Adawiyah, Rabi’atul Aditia Aditia Aditia, Aditia Affifatuzahara Affifatuzahara Affifatuzahara, Affifatuzahara Al-qorifah, Fahma Ajeng Alfianita, Riska Anisya Nur Shafitri Anjelica, Benedikta Annisa, Liza Harum Nur Aprilia, Dinasti Ardhani, Adha Riesta Arifah, Rizki Zanuba Artanti, Lina Yunda Astuti, Fitri Mega Aufani, Destia Aulia, Annisa Syafa Azura, Azura Azzahra, Larasabella Bagus Muhammad Ihsan Benedikta Anjelica Cholsakhon, Panyada Desi Wahyumarniasari Devi Nurfitri Bintang Dinasti Aprillia Djohan, Herlinda Edi Santoso Fadilla, Alda Nur Fahma Ajeng Al-qorifah Fahrani, Chantika Lailatussa'adah Fitra Fathuhudin Grovindo, Grovindo Hartati, Fitri Ulya Hendra Budi Sungkawa, Hendra Budi Herlinda Djohan Ihsan, Bagus Muhammad Imma Fatayati Indah Purwaningsih Indah Purwaningsih Indrawati, Ratih Jessica Ningtyas Berlianti Jordan, Aristo Juliana Cristyaningsih Kartini Kartini Laila Kamilla Linda Triana Maretalinia Maretalinia Maretalinia, Maretalinia Maulidiyah Salim Merda Sri Rahayu Monalita, Ria Muhammad Ihsan, Bagus Munardi, Fhatia Natasya Natasya Intan Ramadhani Nurhayati, Etiek Nurul Uq'ma P Purwanto Perdede, Lusi Hernawati Pratiwi, Tarisya Fitri Rahayu, Merda Sri Rahmatika, Tantri Ramadan, Aditiya Ratnawaty, Gervacia Jenny Ratulangi, Agus Dimas Reza Setiawan Reza, Nur Rezky, Muhammad Andyka Riska Alfianita Rizki Zanuba Arifah Rizki, Sri Yuniarti Romadi Romadi Sadid, Randy Samwidya, Veronika Sriayu Sari, Emilda Sen, Sharen Shafitri, Anisya Nur Slamet Sri Tumpuk Sugito Sugito Sugito Sugito Supriyanto Supriyanto Sutriswanto, Sutriswanto Suwandi, Edy Syahdilla, Ade Syopingi Syopingi Syopingi, Syopingi Theresia Indah Budhy Sulisetyawati Tika, Dian Tumpuk, Sri Uq'ma, Nurul Veny Veny Wahdaniah Wahyudi Wicaksono, Didik Yunus, Mohd