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All Journal Journal Of Vocational Health Studies Klinikal Sains (Jurnal Analis Kesehatan) Care : Jurnal Ilmiah Ilmu Kesehatan Jurnal Riset Kesehatan Poltekkes Depkes Bandung INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Ners Jurnal teknologi Kesehatan Borneo MAHESA : Malahayati Health Student Journal Jurnal EduHealth JURNAL ILMIAH GLOBAL EDUCATION Innovative: Journal Of Social Science Research Jurnal Vokasi Kesehatan Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Media Penelitian dan Pengembangan Kesehatan MEDICA (International Medical Scientific Journal) Jurnal Info Kesehatan ELECTRON (Journal of Science and Technology)
Ari Nuswantoro
Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
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Potential of Methanol Extract of Bungli Fruit Seed (Oroxylum Indicum) in Inhibiting the Growth of Escherichia coli Nuswantoro, Ari; Natasya Intan Ramadhani
Jurnal teknologi Kesehatan Borneo Vol 2 No 1 (2021): Jurnal Teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v2i1.29

Abstract

Bungli (Oroxylum indicum) has been used for generations in Asia as traditional medicine. The seeds of the bungli are commonly used as traditional medicine to treat typhoid, cough, hypertension, internal fever, fever and diarrhea. The active compounds in this plant are known to have anti-inflammatory, antirheumatic, antifungal and antibacterial activities. Our research on methanol extract of bungli fruit seeds showed its potential to inhibit the growth of Escherichia coli bacteria that causes diarrhea. Bungli seed extract samples with a concentration of 25% showed an inhibition zone with a diameter of 9.22 mm, while at a concentration of 50% the diameter was 11.16 mm and 75% had a diameter of 12.88 mm. This size is indeed smaller than chloramphenicol (26 mm), but this potential can be greater if the extraction is carried out more intensively to extract the active compound, the sample concentration is increased, and the part or age of the plant with the highest content of the active compound is used; so that the Bungli plant is expected to be an alternative in overcoming Escherichia coli infection.
PERBEDAAN HASIL HITUNG BERBAGAI JENIS LEUKOSIT PADA PASIEN COVID-19 YANG ISOLASI MANDIRI DENGAN ISOLASI DI RSUD KABUPATEN MELAWI Reza, Nur; Santoso, Edi; Nuswantoro, Ari; Indrawati, Ratih; Nurhayati, Etiek; Aditia, Aditia
Media Penelitian dan Pengembangan Kesehatan Vol. 32 No. 3 (2022): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v32i3.1886

Abstract

COVID-19 infection causes various clinical manifestations so the treatment method is also different for each individual. Patient isolation is one of the treatments to break the chain of transmission of COVID-19. Asymptomatic to moderate symptomatic patients are usually asked to self-isolate, while those with severe and critical symptoms are isolated in hospital. Leukocyte examination is an important parameter and plays a role in determining patient follow-up. The aim of this study was to find out whether there were differences in leukocyte differential-counts in COVID-19 patients who were self-isolating and those isolated at Melawi Regional Hospital. This study had a cross-sectional design by taking data from medical records of COVID-19 patients at Melawi Regional Hospital during October 2021 to July 2022. The data taken were from 399 COVID-19 patients, consisting of 182 patients are self-isolating and 217 patients are isolating at Melawi Regional Hospital. In self-isolated patients, the average value of leukocyte count was 7.04 x 10³ cells/µL, and the leukocyte differential-counts was neutrophils 58.45%, lymphocytes 21.81%, monocytes 13.84%, eosinophils 1.52%, and basophils 4.38%. In isolated patients at Melawi Regional Hospital, the average value of leukocyte count was 9.89 x 10³ cells/µL, and the leukocyte differential-counts was neutrophils 75.09%, lymphocytes 12.97%, monocytes 8.38%, eosinophils 0.46%, and basophils 3.11%. The Mann-Whitney U test obtained a significance value of 0.000 (p < 0.05), which means there is a significant difference in the results of leukocyte counts between self-isolated patients and those isolated at Melawi Regional Hospital.
KAJIAN TENTANG PENURUNAN JUMLAH DONOR DARAH AKIBAT PANDEMI COVID-19: DATA SELAMA 4 TAHUN DARI UDD PMI KOTA PONTIANAK Aditia, Aditia; Samwidya, Veronika Sriayu; Nuswantoro, Ari; Djohan, Herlinda; Supriyanto, Supriyanto; Reza, Nur
Media Penelitian dan Pengembangan Kesehatan Vol. 32 No. 4 (2022): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v32i4.1890

Abstract

The COVID-19 pandemic has left a noticeable mark on the health care sector. One such example is the blood services provided by the Blood Donor Unit (UDD) of Indonesian Red Cross (PMI). Every time there was an increase in COVID-19 cases, blood donation activities sharply decreased, according to the UDD PMI. During the COVID-19 pandemic, there was a scarcity of blood donors. This research aimed to see the difference in the number of blood donors before and during the pandemic. This research was descriptive analytical with a cross-sectional design by collecting data on the number of blood donors at UDD PMI Pontianak City from 2018 to 2022. The results of the study show that the number of blood donors before the pandemic was 52,607 donors, while the number of blood donors during the pandemic was 48,421 donors. which means there was a decrease in the number of blood donors by 4,186 donors (7.96%). The Independent Samples Test obtained a significant value of 0.045 (p < 0.05), which means there was a difference in the number of blood donors before and during the COVID-19 pandemic at UDD PMI Pontianak City. Concerns about the risk of infection and government policies limiting activities outside the home can explain why the COVID-19 pandemic has resulted in a decline in blood donation rates.
KORELASI KADAR C-REACTIVE PROTEIN DENGAN JUMLAH TROMBOSIT PADA PENDERITA DIABETES MELITUS TIPE 2 Nuswantoro, Ari; Wicaksono, Didik; Aditia, Aditia
Media Penelitian dan Pengembangan Kesehatan Vol. 33 No. 2 (2023): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v33i2.1903

Abstract

Type 2 diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia and insulin resistance, associated with decreased nitric oxide (NO) levels, increased free radical production, inflammation, endothelial dysfunction, and progressive vascular damage. C-reactive protein (CRP), an acute phase protein regulated by pro-inflammatory cytokines like interleukin-6 (IL-6), serves as a systemic inflammation marker. Pentameric CRP (pCRP) and monomeric CRP (mCRP) are implicated in mediating inflammation and endothelial dysfunction, with roles in endothelial cell adhesion, phagocytosis, inflammatory signaling, and interactions with endothelial cells, neutrophils, macrophages, and platelets. Additionally, leukocytes secrete platelet-activating factors, enhancing platelet activation and cytokine and chemokine release. This descriptive analytical study with a cross-sectional design aimed to investigate the correlation between CRP levels and platelet counts in patients with type 2 DM. Utilizing purposive sampling, 47 patients diagnosed with DM at Sultan Syarif Mohamad Alkadrie Hospital, Pontianak, during June 2023 were selected. CRP levels were measured using the fluorescence immunoassay (FIA) method, while platelet counts were determined through the impedance method. Statistical analysis employing Kendall's Tau-b test revealed a significant correlation (p < 0.05, r = -0.508), indicating that elevated CRP levels are significantly associated with reduced platelet counts in type 2 DM patients.
EFEKTIVITAS MENGONSUMSI OBAT ANTIRETROVIRAL DALAM MENURUNKAN ANGKA VIRAL LOAD PADA ODHA Fahrani, Chantika Lailatussa'adah; Romadi, Romadi; Nuswantoro, Ari; Wahdaniah, Wahdaniah; Aditia, Aditia
Media Penelitian dan Pengembangan Kesehatan Vol. 33 No. 1 (2023): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v33i1.1934

Abstract

Human Immunodeficiency Virus (HIV) has infected many people throughout the world. In Indonesia alone, around 540,000 people have been infected with HIV and are living with Acquired Immunodeficiency Syndrome (AIDS). Until now, antiretroviral drug therapy (ARV) is the best choice which aims to reduce the risk of transmission, prevent worsening due to opportunistic infections, improve the quality of life of sufferers, and reduce the amount of virus (viral load) in the blood until it is undetectable. The aim of this study was to explain the effectiveness of taking ARV drugs in reducing viral load rates in patients at the Tulipe Clinic, M. Th. Hospital. Djaman, Sanggau Regency. This is a non-experimental quantitative research with a cross-sectional design. Sampling technique using purposive sampling, with the criteria applied, namely that respondents are ODHA or patients who have been diagnosed with HIV/AIDS, have undergone ARV therapy for at least six months, and are willing to become respondents so that a total of 89 people during 2022 – 2023 were obtained. The results of the study showed that the duration of taking ARVs ranged from 6 to 98 months, the viral load number was 14 - 35,400 copies/ml. The Spearman test obtained a significance value of 0.000 (p < 0.05), which means that taking ARV medication is effective in reducing viral load rates in Tulipe Clinic patients at M. Th. Hospital. Djaman, Sanggau Regency. Based on the results, it is expected that future studies can measure viral load along with molecular immunological parameters such as cytokine and antibody levels.
HUBUNGAN KADAR C-REACTIVE PROTEIN DENGAN DERAJAT KEPARAHAN PASIEN COVID-19 YANG DIRAWAT INAP DI RUMAH SAKIT Nuswantoro, Ari; Perdede, Lusi Hernawati; Aulia, Annisa Syafa; Aditia, Aditia
Media Penelitian dan Pengembangan Kesehatan Vol. 32 No. 4 (2022): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v32i4.2026

Abstract

Coronavirus Disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV2) can appear with a varied clinical spectrum, ranging from no symptoms, mild illness, moderate illness, to severe illness. The high mortality rate is closely related to the severity of the disease. One of the laboratory parameters that is thought to be related to the severity of COVID-19 is C-Reactive Protein (CRP). This study aimed to determine the relationship between CRP values and the severity of COVID-19 patients. This research has an analytical observational design with a cross-sectional approach. The samples were COVID-19 patients with moderate and severe symptoms who were hospitalized at RSUD dr. Soedarso West Kalimantan in January - December 2021. The sampling technique was total sampling to obtain medical record data which included CRP values and patient severity levels so that 49 data were obtained. The results showed that 8 respondents (16.3%) had CRP levels of 0.8 mg/dL, 24 respondents (49%) had CRP levels of 1.6 mg/dL, 9 respondents (18.4%) had CRP levels of 3 .2 mg/dL and 8 respondents (16.3%) had CRP levels of 6.4 mg/dL. Based on Kendall's tau-b statistical test, a significance value of 0.001 <0.05 was obtained, which means there is a significant relationship between CRP levels and severity. The correlation coefficient value of 0.708 shows a strong correlation coefficient between CRP levels and severity. This proves that the higher the CRP levels of COVID-19 patients found, the more severe the degree of disease that may be suffered by these patients, so there is a close relationship between CRP levels and the degree of disease of COVID-19 patients.
KORELASI JUMLAH MONOSIT DENGAN KADAR C-REACTIVE PROTEIN PADA PENDERITA TUBERKULOSIS Nuswantoro, Ari; Astuti, Fitri Mega; Hartati, Fitri Ulya; Aditia, Aditia
Media Penelitian dan Pengembangan Kesehatan Vol. 32 No. 3 (2022): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v32i3.2027

Abstract

Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) is still a public health problem throughout the world, including in Indonesia. Monocytes play a role in cellular reactions against MTB bacteria and are considered a marker of active TB spread. C-Reactive Protein (CRP) examination is very useful for diagnosing inflammation or infectious diseases. This study aimed to determine the correlation between monocyte counts and CRP levels in TB patients in North Putussibau. This research was observational analytic with cross-sectional approach and the sampling technique was accidental nonprobability sampling. The sample was TB patients in North Putussibau who were undergoing treatment for 0, 2, and 6 months in 2022, resulting in 36 patients. Monocyte counts were examined using the fluorescence method and CRP levels were examined using the latex agglutination method. At 0 months of treatment, the monocyte count was obtained with an average of 1.096.88cells/µL and average CRP level of 55.13mg/L, at 2 months of treatment the average monocyte count was 742.31 cells/µL and CRP levels were obtained an average of 10.62mg/L, and after 6 months of treatment an average monocyte count was 414.29cells/µL and an average CRP level of 0mg/L. The Kendall's Tau-b test showed a significant value 0.000 (<0.05), which means there was a significant correlation between the number of monocytes and CRP levels in TB patients with a correlation coefficient 0.645 which shows the strength of  strong relationship. This shows that the decrease in the number of monocytes and CRP levels is in line with the length of the TB treatment period.
KORELASI ANTARA RASIO NEUTROFIL/LIMFOSIT DENGAN KADAR C-REACTIVE PROTEIN PADA PENDERITA TUBERKULOSIS PARU Aufani, Destia; Azzahra, Larasabella; Supriyanto, Supriyanto; Fatayati, Imma; Nuswantoro, Ari
Media Penelitian dan Pengembangan Kesehatan Vol. 33 No. 2 (2023): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v33i2.2089

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis and can persist in all parts of the body. Laboratory tests used to determine the level of inflammation in TB sufferers include the neutrophil/lymphocyte ratio (RNL) and C-reactive protein (CRP) levels. The RNL value is the ratio of the absolute number of neutrophils and the absolute number of lymphocytes, while CRP is an acute phase protein produced in the liver, and its levels increase within 6 hours in acute inflammation. The aim of this study was to determine the relationship between RNL and CRP in pulmonary TB patients. This research was a descriptive study with a cross-sectional design carried out from April to June 2021 at the Lung Health Services Integrated Service Unit, West Kalimantan Province. The number of samples was determined using a purposive sampling technique, with the criteria being newly diagnosed TB patients who had positive BTA, positive TCM and positive X-ray results, resulting in 48 samples. CRP levels were measured using the latex agglutination method, while the number of neutrophils and lymphocytes used the impedance method. Analysis was carried out using the Shapiro-Wilk normality test and Kendall's tau b correlation test. Statistical analysis using the Kendall's tau b correlation test obtained a significant value of 0.000 (<0.05), which means there was a relationship between RNL and CRP levels in pulmonary TB patients with a correlation coefficient of 0.489, which means the relationship between the two variables is enough.
EDUKASI PENYAKIT TB PARU DAN SOSIALISASI PENGUMPULAN SAMPEL SPUTUM DIAGNOSIS TUBERCULOSIS Salim, Maulidiyah; Muhammad Ihsan, Bagus; Nuswantoro, Ari; Indrawati, Ratih; Jenny Ratnawati, Gervacia
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 2 (2025): MAJU : Indonesian Journal of Community Empowerment, Maret 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i2.1075

Abstract

Tuberkulosis (TBC) adalah suatu penyakit menular yang disebabkan oleh kuman Mycobacterium tuberculosis. Kuman tersebut biasanya masuk kedalam tubuh manusia melalui udara pernapasan kedalam paru. Kemudian kuman tersebut dapat menyebar melalui udara pernapasan ke dalam paru, kemudian kuman tersebut dapat menyebar dari paru ke bagian tubuh lainya melalui sistem peredaran darah, sistem saluran limfa, melalui saluran pernapasan atau penyebaran langsung kebagian tubuh lainnya. Tuberkulosis masih merupakan masalah kesehatan masyarakat yang menjadi tantangan global. Secara global kasus baru tuberkulosis sebesar 6,4 juta. Setara dengan 64 % dari insiden tuberkulosis (10 juta). Tuberkulosis tetap menjadi 10 penyebab kematian tertinggi di dunia dan kematian tuberkulosis secara global diperkirakan 1,3 juta pasien. Pengabdian masyarakat ini bertujuan untuk upaya pencegahan dengan melakukan penyuluhan kepada masyarakat masih sangat jarang dilakukan, sementara lingkungan kehidupan para masyarakat berada dalam kelompok masyarakat dengan kepadatan hunian yang tinggi. Pencegahan penyakit dan promosi kesehatan tentang Tuberkulosis merupakan upaya esensial disaat masih tingginya kejadian penyakit menular juga di iringi dengan semakin tinggi masalah penyakit tidak menular. Metode pengabdian kepada masyarakat ini merujuk pada bagian tujuan diatas, Secara umum sasaran yang ingin dicapai dari kegiatan ini banyak melibatkan banyak pihak demi mencapai tujuan diatas. Kegiatan pengabdian ini akan fokus pada pemberian edukasi kepada masyarakat tentang upaya yang dapat dilakukan untuk mencegah penyakit tuberkulosis, cara penularannya serta cara pengambilan spesimen sputum dengan baik dan benar. Kegiatan yang sudah dilakukan adalah Edukasi Penyakit TBC serta Sosialisasi mengenai pengumpulan sampel sputum yang dilakukan di daerah binaan di Desa Rasau Jaya Kabupaten Kubu Raya, kegiatan pengabdian masyarakat ini dihadiri sekitar 35 orang dalam waktu kurang lebih 2 jam dimana pengabdian masyarakat ini dilakukan dalam bentuk penyuluhan dan diskusi tanya jawab.
ANTHOCYANIN-CONTAINING PLANT EXTRACTS AS AN ALTERNATIVE DYE FOR MICROSCOPIC EXAMINATION OF SOIL-TRANSMITTED HELMINTHS Ari Nuswantoro; Riska Alfianita; Fitra Fathuhudin; Veny Veny
Journal of Vocational Health Studies Vol. 8 No. 3 (2025): March 2025 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V8.I3.2025.183-190

Abstract

Background: Eosin is commonly used for microscopic examination of Soil-Transmitted Helminth (STH) infections, but natural anthocyanin-based pigments remain underutilized. Purpose: This study evaluates the potential of anthocyanins extracted from red beans (Phaseolus vulgaris), hibiscus flowers (Hibiscus rosa-sinensis), and amaranth leaves (Amaranthus tricolor) as eosin alternatives for staining STH eggs in stool smears. Method: Extracts were obtained using 96% ethanol for 24, 48, and 72 hours, with nine replications. Stool preparations were stained with these extracts and compared to eosin, assessing color intensity, contrast, and egg layer clarity. Result: Red bean extracts scored 2.1, 2.7, and 2.8 at 24, 48, and 72 hours, with the latter two showing no significant difference from eosin. Hibiscus flower extracts scored 1.6, 2.2, and 2.8, with the 72 hours extract comparable to eosin. Amaranth leaf extracts scored 1.4, 1.7, and 1.9, all significantly different from eosin. Conclusion: Red bean extracts (48 and 72 hours) and hibiscus flower extract (72 hours) provided staining comparable to eosin, with red bean extract being the most promising alternative. These findings suggest that anthocyanin-based stains can serve as viable substitutes for eosin in diagnosing helminthiasis via stool smear microscopy.
Co-Authors Adawiyah, Rabi’atul Aditia Aditia Aditia, Aditia Affifatuzahara Affifatuzahara Affifatuzahara, Affifatuzahara Al-qorifah, Fahma Ajeng Alfianita, Riska Anisya Nur Shafitri Anjelica, Benedikta Annisa, Liza Harum Nur Aprilia, Dinasti Ardhani, Adha Riesta Arifah, Rizki Zanuba Artanti, Lina Yunda Astuti, Fitri Mega Aufani, Destia Aulia, Annisa Syafa Azura, Azura Azzahra, Larasabella Bagus Muhammad Ihsan Benedikta Anjelica Cholsakhon, Panyada Desi Wahyumarniasari Devi Nurfitri Bintang Dinasti Aprillia Djohan, Herlinda Edi Santoso Fadilla, Alda Nur Fahma Ajeng Al-qorifah Fahrani, Chantika Lailatussa'adah Fitra Fathuhudin Grovindo, Grovindo Hartati, Fitri Ulya Hendra Budi Sungkawa, Hendra Budi Herlinda Djohan Ihsan, Bagus Muhammad Imma Fatayati Indah Purwaningsih Indah Purwaningsih Indrawati, Ratih Jessica Ningtyas Berlianti Jordan, Aristo Juliana Cristyaningsih Kartini Kartini Laila Kamilla Linda Triana Maretalinia Maretalinia Maretalinia, Maretalinia Maulidiyah Salim Merda Sri Rahayu Monalita, Ria Muhammad Ihsan, Bagus Munardi, Fhatia Natasya Natasya Intan Ramadhani Nurhayati, Etiek Nurul Uq'ma P Purwanto Perdede, Lusi Hernawati Pratiwi, Tarisya Fitri Rahayu, Merda Sri Rahmatika, Tantri Ramadan, Aditiya Ratnawaty, Gervacia Jenny Ratulangi, Agus Dimas Reza Setiawan Reza, Nur Rezky, Muhammad Andyka Riska Alfianita Rizki Zanuba Arifah Rizki, Sri Yuniarti Romadi Romadi Sadid, Randy Samwidya, Veronika Sriayu Sari, Emilda Sen, Sharen Shafitri, Anisya Nur Slamet Sri Tumpuk Sugito Sugito Sugito Sugito Supriyanto Supriyanto Sutriswanto, Sutriswanto Suwandi, Edy Syahdilla, Ade Syopingi Syopingi Syopingi, Syopingi Theresia Indah Budhy Sulisetyawati Tika, Dian Tumpuk, Sri Uq'ma, Nurul Veny Veny Wahdaniah Wahyudi Wicaksono, Didik Yunus, Mohd