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All Journal Journal Of Vocational Health Studies Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Riset Kesehatan Poltekkes Depkes Bandung INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Ners Jurnal teknologi Kesehatan Borneo MAHESA : Malahayati Health Student Journal Jurnal EduHealth JURNAL ILMIAH GLOBAL EDUCATION Innovative: Journal Of Social Science Research Jurnal Vokasi Kesehatan Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Media Penelitian dan Pengembangan Kesehatan MEDICA (International Medical Scientific Journal) Jurnal Info Kesehatan ELECTRON (Journal of Science and Technology)
Ari Nuswantoro
Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
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KORELASI JUMLAH MONOSIT DENGAN KADAR C-REACTIVE PROTEIN PADA PENDERITA TUBERKULOSIS Nuswantoro, Ari; Astuti, Fitri Mega; Hartati, Fitri Ulya; Aditia, Aditia
Media Penelitian dan Pengembangan Kesehatan Vol. 32 No. 3 (2022): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v32i3.2027

Abstract

Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) is still a public health problem throughout the world, including in Indonesia. Monocytes play a role in cellular reactions against MTB bacteria and are considered a marker of active TB spread. C-Reactive Protein (CRP) examination is very useful for diagnosing inflammation or infectious diseases. This study aimed to determine the correlation between monocyte counts and CRP levels in TB patients in North Putussibau. This research was observational analytic with cross-sectional approach and the sampling technique was accidental nonprobability sampling. The sample was TB patients in North Putussibau who were undergoing treatment for 0, 2, and 6 months in 2022, resulting in 36 patients. Monocyte counts were examined using the fluorescence method and CRP levels were examined using the latex agglutination method. At 0 months of treatment, the monocyte count was obtained with an average of 1.096.88cells/µL and average CRP level of 55.13mg/L, at 2 months of treatment the average monocyte count was 742.31 cells/µL and CRP levels were obtained an average of 10.62mg/L, and after 6 months of treatment an average monocyte count was 414.29cells/µL and an average CRP level of 0mg/L. The Kendall's Tau-b test showed a significant value 0.000 (<0.05), which means there was a significant correlation between the number of monocytes and CRP levels in TB patients with a correlation coefficient 0.645 which shows the strength of  strong relationship. This shows that the decrease in the number of monocytes and CRP levels is in line with the length of the TB treatment period.
KORELASI ANTARA RASIO NEUTROFIL/LIMFOSIT DENGAN KADAR C-REACTIVE PROTEIN PADA PENDERITA TUBERKULOSIS PARU Aufani, Destia; Azzahra, Larasabella; Supriyanto, Supriyanto; Fatayati, Imma; Nuswantoro, Ari
Media Penelitian dan Pengembangan Kesehatan Vol. 33 No. 2 (2023): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v33i2.2089

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis and can persist in all parts of the body. Laboratory tests used to determine the level of inflammation in TB sufferers include the neutrophil/lymphocyte ratio (RNL) and C-reactive protein (CRP) levels. The RNL value is the ratio of the absolute number of neutrophils and the absolute number of lymphocytes, while CRP is an acute phase protein produced in the liver, and its levels increase within 6 hours in acute inflammation. The aim of this study was to determine the relationship between RNL and CRP in pulmonary TB patients. This research was a descriptive study with a cross-sectional design carried out from April to June 2021 at the Lung Health Services Integrated Service Unit, West Kalimantan Province. The number of samples was determined using a purposive sampling technique, with the criteria being newly diagnosed TB patients who had positive BTA, positive TCM and positive X-ray results, resulting in 48 samples. CRP levels were measured using the latex agglutination method, while the number of neutrophils and lymphocytes used the impedance method. Analysis was carried out using the Shapiro-Wilk normality test and Kendall's tau b correlation test. Statistical analysis using the Kendall's tau b correlation test obtained a significant value of 0.000 (<0.05), which means there was a relationship between RNL and CRP levels in pulmonary TB patients with a correlation coefficient of 0.489, which means the relationship between the two variables is enough.
EDUKASI PENYAKIT TB PARU DAN SOSIALISASI PENGUMPULAN SAMPEL SPUTUM DIAGNOSIS TUBERCULOSIS Salim, Maulidiyah; Muhammad Ihsan, Bagus; Nuswantoro, Ari; Indrawati, Ratih; Jenny Ratnawati, Gervacia
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 2 (2025): MAJU : Indonesian Journal of Community Empowerment, Maret 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i2.1075

Abstract

Tuberkulosis (TBC) adalah suatu penyakit menular yang disebabkan oleh kuman Mycobacterium tuberculosis. Kuman tersebut biasanya masuk kedalam tubuh manusia melalui udara pernapasan kedalam paru. Kemudian kuman tersebut dapat menyebar melalui udara pernapasan ke dalam paru, kemudian kuman tersebut dapat menyebar dari paru ke bagian tubuh lainya melalui sistem peredaran darah, sistem saluran limfa, melalui saluran pernapasan atau penyebaran langsung kebagian tubuh lainnya. Tuberkulosis masih merupakan masalah kesehatan masyarakat yang menjadi tantangan global. Secara global kasus baru tuberkulosis sebesar 6,4 juta. Setara dengan 64 % dari insiden tuberkulosis (10 juta). Tuberkulosis tetap menjadi 10 penyebab kematian tertinggi di dunia dan kematian tuberkulosis secara global diperkirakan 1,3 juta pasien. Pengabdian masyarakat ini bertujuan untuk upaya pencegahan dengan melakukan penyuluhan kepada masyarakat masih sangat jarang dilakukan, sementara lingkungan kehidupan para masyarakat berada dalam kelompok masyarakat dengan kepadatan hunian yang tinggi. Pencegahan penyakit dan promosi kesehatan tentang Tuberkulosis merupakan upaya esensial disaat masih tingginya kejadian penyakit menular juga di iringi dengan semakin tinggi masalah penyakit tidak menular. Metode pengabdian kepada masyarakat ini merujuk pada bagian tujuan diatas, Secara umum sasaran yang ingin dicapai dari kegiatan ini banyak melibatkan banyak pihak demi mencapai tujuan diatas. Kegiatan pengabdian ini akan fokus pada pemberian edukasi kepada masyarakat tentang upaya yang dapat dilakukan untuk mencegah penyakit tuberkulosis, cara penularannya serta cara pengambilan spesimen sputum dengan baik dan benar. Kegiatan yang sudah dilakukan adalah Edukasi Penyakit TBC serta Sosialisasi mengenai pengumpulan sampel sputum yang dilakukan di daerah binaan di Desa Rasau Jaya Kabupaten Kubu Raya, kegiatan pengabdian masyarakat ini dihadiri sekitar 35 orang dalam waktu kurang lebih 2 jam dimana pengabdian masyarakat ini dilakukan dalam bentuk penyuluhan dan diskusi tanya jawab.
ANTHOCYANIN-CONTAINING PLANT EXTRACTS AS AN ALTERNATIVE DYE FOR MICROSCOPIC EXAMINATION OF SOIL-TRANSMITTED HELMINTHS Ari Nuswantoro; Riska Alfianita; Fitra Fathuhudin; Veny Veny
Journal of Vocational Health Studies Vol. 8 No. 3 (2025): March 2025 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V8.I3.2025.183-190

Abstract

Background: Eosin is commonly used for microscopic examination of Soil-Transmitted Helminth (STH) infections, but natural anthocyanin-based pigments remain underutilized. Purpose: This study evaluates the potential of anthocyanins extracted from red beans (Phaseolus vulgaris), hibiscus flowers (Hibiscus rosa-sinensis), and amaranth leaves (Amaranthus tricolor) as eosin alternatives for staining STH eggs in stool smears. Method: Extracts were obtained using 96% ethanol for 24, 48, and 72 hours, with nine replications. Stool preparations were stained with these extracts and compared to eosin, assessing color intensity, contrast, and egg layer clarity. Result: Red bean extracts scored 2.1, 2.7, and 2.8 at 24, 48, and 72 hours, with the latter two showing no significant difference from eosin. Hibiscus flower extracts scored 1.6, 2.2, and 2.8, with the 72 hours extract comparable to eosin. Amaranth leaf extracts scored 1.4, 1.7, and 1.9, all significantly different from eosin. Conclusion: Red bean extracts (48 and 72 hours) and hibiscus flower extract (72 hours) provided staining comparable to eosin, with red bean extract being the most promising alternative. These findings suggest that anthocyanin-based stains can serve as viable substitutes for eosin in diagnosing helminthiasis via stool smear microscopy.
PROFIL LIPID SEBAGAI PREDIKTOR UREUM DAN KREATININ PADA PASIEN PROLANIS PENDERITA DMT2 DI UPT PUSAT LABKES KOTA PONTIANAK Tarisya Fitri Pratiwi; Imma Fatayati; Supriyanto Supriyanto; Ari Nuswantoro; Dinasti Aprillia
Jurnal Review Pendidikan dan Pengajaran Vol. 7 No. 4 (2024): Vol. 7 No. 4 Tahun 2024
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v7i4.33968

Abstract

Lebih dari 1 miliar orang diperkirakan akan menderita diabetes melitus (DM) pada tahun 2050. Keadaan dislipidemia akibat DM menaikkan risiko inisiasi komplikasi nefropati diabetik. Dislipidemia memiliki dampak tidak langsung terhadap penurunan fungsi ginjal karena dapat menyebabkan aterosklerosis, yang membuat ginjal bekerja lebih keras untuk menyaring darah dari waktu ke waktu. Tujuan penelitian ini adalah untuk mengetahui apakah profil lipid berhubungan dengan ureum dan kreatinin sebagai parameter fungsi ginjal pada pasien prolanis penderita DMT 2 yang terdaftar di Unit Pelaksana Teknis (UPT) Pusat Laboratorium Kesehatan Kota Pontianak. Penelitian ini berbentuk deskriptif dan berdesain cross-sectional dengan menggunakan data sekunder rekam medis pasien periode Januari 2023 sampai dengan Desember 2023 pasien prolanis penderita DMT 2 yang terdaftar di UPT Pusat Laboratorium Kesehatan Kota Pontianak yang dipilih menggunakan teknik purposive sampling, dengan kriteria yang diberlakukan yaitu pasien prolanis penderita DMT 2 yang melakukan pemeriksaan kolesterol total, trigliserida, HDL, LDL, ureum dan kreatinin dan terdaftar di UPT Pusat Laboratorium Kesehatan Kota Pontianak sehingga didapatkan 339 responden. Analisis statistik dengan uji Kendall’s Tau-b menunjukkan kadar HDL berhubungan dengan kadar ureum dan kadar kreatinin dengan nilai signifikansi berturut-turut adalah 0,035 (p <0,05) dan 0,001 (p < 0,01). Serta didapatkan nilai koefisien korelasi berturut-turut adalah r = -0,078 dan -0,123 yang artinya semakin rendah kadar HDL maka semakin tinggi kadar ureum dan kadar kreatinin pasien tersebut.
The Relationship Between Premature Rupture of Membranes (PROM) and Leukocyte Levels in Newborns Grovindo Grovindo; Sutriswanto Sutriswanto; Ari Nuswantoro; Panyada Cholsakhon
MEDICA (International Medical Scientific Journal) Vol. 7 No. 1 (2025): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i1.484

Abstract

Premature Rupture of Membranes (PROM) remains a global concern in obstetrics due to its potential to cause complications such as infections that increase maternal and neonatal morbidity and mortality. PROM occurs in approximately 10–15% of pregnancies worldwide and 8–10% in Indonesia, often before 37 weeks of gestation, contributing significantly to preterm births and neonatal deaths. Risk factors for PROM include maternal age, parity, infection, and other pregnancy-related conditions. Infection resulting from PROM can be identified through leukocyte count in newborns, with leukocytosis indicating the presence of infection. This study aimed to examine the relationship between PROM and leukocyte levels in newborns at RSUD Drs. Jacobus Luna, M.Si, Bengkayang Regency, using a descriptive-analytic design with a cross-sectional approach and total sampling of 88 respondents. Results showed that among 36 newborns with elevated leukocyte levels, 28 cases (77.8%) were born from PROM pregnancies, whereas only 8 cases (22.2%) were non-PROM. Among 52 cases with normal leukocyte levels, only 11 newborns (21.2%) had PROM, while 41 (78.8%) did not. These findings indicate that newborns from PROM pregnancies tend to have higher leukocyte counts. Statistical analysis using Chi-square showed a significant relationship between PROM and leukocyte levels, with a p-value of 0.000 (< 0.05). In conclusion, PROM is a notable risk factor for increased leukocyte counts in neonates, underscoring the importance of early detection and management of PROM to reduce the risk of neonatal infection and its associated complications.
The Antibacterial Effectiveness of Moringa Oleifera Leaves Extract Against the Growth of Escherichia coli Using the Diffusion Method Liza Harum Nur Annisa; Linda Triana; Emilda Sari; Ari Nuswantoro
MEDICA (International Medical Scientific Journal) Vol. 6 No. 3 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i3.487

Abstract

Moringa oleifera leaves a common herbal plant found in tropical regions and naturalized in Indonesia, are known to be rich in bioactive compounds such as flavonoids, tannins, terpenoids, alkaloids, and saponins. These compounds have potential medicinal properties, including antibacterial activity. This study aims to examine the antibacterial effectiveness of moringa leaf extract against the growth of Escherichia coli using the diffusion method. This descriptive-analytical research employed a purposive sampling technique with varying concentrations of moringa leaf extract: 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. Each concentration was tested in triplicate, resulting in a total of 30 samples. The antibacterial effectiveness test was conducted using the agar diffusion method with blank paper disks. The results showed that no inhibition zones were formed at concentrations of 10% and 20%. Inhibition zones began to appear at 30% concentration (7.3 mm), increasing with higher concentrations: 40% (9 mm), 50% (10.3 mm), 60% (11.3 mm), 70% (16.6 mm), 80% (23.3 mm), 90% (28.3 mm), and reaching 34.6 mm at 100% concentration. Based on the antibacterial activity category, 10% and 20% showed no activity, 30% and 40% were categorized as weak, 50% and 60% as moderate, 70% as strong, and 80%, 90%, and 100% as very strong. It can be concluded that an 80% concentration of moringa leaf extract is the most effective in inhibiting the growth of Escherichia coli.
The Effect of Different Soaking Times of Gambir (Uncaria gambir (Hunter) Roxb.) on the Examination of Ascaris lumbricoides Eggs as an Alternative Stain to Eosin Tantri Rahmatika; Ari Nuswantoro; Laila Kamilla
MEDICA (International Medical Scientific Journal) Vol. 7 No. 2 (2025): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i2.512

Abstract

Ascaris lumbricoides is a type of helminth that can infect humans. One laboratory method for its examination is the use of 2% eosin staining with the direct slide method. However, eosin poses potential hazards to both the environment and human health, thus prompting the need for an alternative, eco-friendly staining agent. Gambir (Uncaria gambir (Hunter) Roxb.) contains red catechu, which imparts a red coloration to solutions. This study aims to evaluate the staining differences based on varying soaking times of Gambir extract for staining Ascaris lumbricoides eggs as a natural alternative to eosin. This research is a quasi-experimental study using purposive sampling. The Gambir samples were soaked in 96% ethanol for 1 hour, 1 hour 30 minutes, and 2 hours. Each treatment was replicated 9 times, resulting in a total of 27 samples. The staining was conducted using the direct slide method to examine Ascaris lumbricoides eggs. The results showed that Gambir soaked for 1 hour yielded a staining quality of 41.67%, 1 hour 30 minutes resulted in 68.51%, and 2 hours resulted in 87.96%, while the 2% eosin control achieved a staining quality of 100%. Based on the results of this study on the variation in soaking time of gambier (Uncaria gambir (Hunter) Roxb.) as an alternative dye to eosin in the examination of Ascaris lumbricoides eggs, it can be concluded that the staining success rate increases with longer soaking durations.
The Effectiveness of Chocolate in Reducing the Number of Methicillin-Resistant Staphylococcus aureus Colonies in Rattus norvegicus Edy Suwandi; Ari Nuswantoro; Sugito Sugito; Desi Wahyumarniasari; Muhammad Reza Setiawan; Dinasti Aprillia; Devi Nurfitri Bintang
JURNAL INFO KESEHATAN Vol 19 No 2 (2021): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol19.Iss2.545

Abstract

Chocolate has long been understood to provide positive emotions and a good mood if consumed in moderation. Chocolate contains prebiotics naturally from its constituent ingredients produced during the production process. Prebiotics, frequently oligosaccharides, are substances which cannot be metabolized by the human digestive system but can be employed by a group of bacteria in the gut, understood as probiotics. The positive relationship among them provides benefits for the host in eliminating pathogens. One of the well-known pathogens which frequently cause infection either in the community or in hospitals is methicillin-resistant Staphylococcus aureus (MRSA). Since it was first identified in 1960, MRSA has caused health problems until today. Research conducted on two groups of Rattus norvegicus infected with MRSA and then fed chocolate revealed a decrease in the average number of bacterial colonies on the skin compared to the control group. In the group fed chocolate at a dose of 50 mg/day, the bacterial colonies decreased to 1.28 x 108 CFU/cm2 in 7 days, lower than in the control group (1.46 x 108 CFU/cm2) at the same time. While those fed 75 mg/day chocolate decreased to 2.70 x 107 CFU/cm2 and the three groups were significantly different (0.000<0.05). Prebiotics fermented by probiotics release short-chain fatty acids (SCFA), which compete with the pathogens for attaching to the epithelial wall so that pathogens lose space and nutrients to survive. However, the adverse effect of chocolate may occur because it contains sugar which is a nutrient for bacteria, but if the balance of normal flora and adequate intake of prebiotics are administered, the pathogen could be eliminated.
The potential of Cilembu sweet potato (Ipomoea batatas L.) as a growth medium for Staphylococcus aureus and Escherichia coli Ari Nuswantoro; Merda Sri Rahayu; Imma Fatayati; Syopingi Syopingi; Maretalinia Maretalinia
JURNAL INFO KESEHATAN Vol 22 No 1 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss1.1087

Abstract

The media aims to store, reproduce, and identify bacteria but has disadvantages such as being expensive, containing chemicals, and being easily damaged due to contamination. Therefore we need an alternative media that can overcome these limitations. Cilembu sweet potato (Ipomoea batatas, L.) is a natural ingredient that is easy to obtain and contains sufficient nutrients so it has the potential to be used as a basic ingredient for growth media. The purpose of this study was to determine the potential of Cilembu sweet potato flour as an alternative medium for the growth of Staphylococcus aureus and Escherichia coli bacteria with a quasi-experimental design. Cilembu sweet potato flour is obtained by cleaning, chopping, drying in the oven, crushing with a blender, and sifting the tubers to obtain fairly fine flour. The flour was dissolved in agar and then inoculated with S. aureus and E. coli, each with 16 replications. The results showed that the average number of S. aureus colonies was 119.12 CFU (169.2 CFU in control) while E. coli was 160.56 CFU (221.2 CFU in control). The Mann-Whitney test showed that there was a difference in the number of S. aureus colonies on alternative media and NA (p = 0.006 ≤ 0.05), but there was no difference between the number of E. coli colonies on alternative media and NA (p = 0.057 > 0.05). Finally, there was a difference in the number of S. aureus and E. coli colonies on alternative media (p = 0.04 ≤ 0.05). The nutritional composition shows that Cilembu sweet potato flour has more potential to replace NA as a growth medium for E. coli than for S. aureus.
Co-Authors Adawiyah, Rabi’atul Aditia Aditia Aditia, Aditia Aditiya Ramadan Affifatuzahara Affifatuzahara Anisya Nur Shafitri Ardhani, Adha Riesta Arifah, Rizki Zanuba Astuti, Fitri Mega Aufani, Destia Aulia, Annisa Syafa Azura azura Azzahra, Larasabella Bagus Muhammad Ihsan Benedikta Anjelica Desi Wahyumarniasari Devi Nurfitri Bintang Dinasti Aprilia Dinasti Aprillia Djohan, Herlinda Edi Santoso Emilda Sari Fadilla, Alda Nur Fahma Ajeng Al-qorifah Fahrani, Chantika Lailatussa'adah Fitra Fathuhudin Grovindo Grovindo Hartati, Fitri Ulya Herlinda Djohan Imma Fatayati Indah Purwaningsih Indah Purwaningsih Indrawati, Ratih Jenny Ratnawati, Gervacia Jessica Ningtyas Berlianti Jordan, Aristo Juliana Cristyaningsih Kartini Kartini Laila Kamilla Laila Kamilla Linda Triana Linda Triana Linda Triana Liza Harum Nur Annisa Maretalinia Maretalinia Maretalinia, Maretalinia Maulidiyah Salim Merda Sri Rahayu Monalita, Ria Muhammad Ihsan, Bagus Munardi, Fhatia Natasya Natasya Intan Ramadhani Nurhayati, Etiek Nurul Uq'ma P Purwanto Panyada Cholsakhon Perdede, Lusi Hernawati Rahayu, Merda Sri Ramadan, Aditiya Ratnawaty, Gervacia Jenny Reza Setiawan Reza, Nur Rezky, Muhammad Andyka Riska Alfianita Rizki Zanuba Arifah Romadi Romadi Sadid, Randy Samwidya, Veronika Sriayu Sharen Sen Slamet Sri Tumpuk Sugito Sugito Sugito Sugito Supriyanto Supriyanto Supriyanto Supriyanto Sutriswanto Sutriswanto Suwandi, Edy Syahdilla, Ade Syopingi Syopingi Syopingi, Syopingi Tantri Rahmatika Tarisya Fitri Pratiwi Theresia Indah Budhy Sulisetyawati Tika, Dian Tumpuk, Sri Veny Veny Wahdaniah Wahyudi Wicaksono, Didik Yunus, Mohd