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Identifikasi Bakteri Coliform pada Minuman Air Tahu yang Dijual di Wilayah Kota Pontianak Rizki, Sri Yuniarti; Jordan, Aristo; Ardhani, Adha Riesta; Nurhayati, Etiek; Nuswantoro, Ari
Innovative: Journal Of Social Science Research Vol. 4 No. 4 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i4.14503

Abstract

Di Kota Pontianak, air tahu merupakan minuman yang populer di semua kalangan, baik anak-anak, remaja, bahkan orang dewasa. Rasanya enak dan menyegarkan. Minuman air tahu yang dipasarkan di Kota Pontianak ditemukan mengandung bakteri coliform yang sangat berbahaya bagi kesehatan. Penelitian ini dilakukan dengan menggunakan metode kultur untuk mengetahui apakah minuman air tahu yang dijual di pinggir jalan di Kota Pontianak terkontaminasi bakteri coliform. Penelitian ini menggunakan desain cross sectional dan berbentuk deskriptif dengan menggunakan teknik incidental sampling. Sebanyak 43 sampel air tahu yang dijual di pinggir jalan di Kota Pontianak digunakan dalam penelitian ini. Dari 43 sampel air tahu yang diperiksa, 17 (39,5%) sampel dinyatakan positif mengandung bakteri coliform, sedangkan 26 (60,5%) sampel lainnya dinyatakan negatif bakteri coliform.
Gambaran Kadar Glukosa Darah Sewaktu Pada Ibu Hamil di Puskesmas Tambelan Sampit Kota Pontianak Ramadan, Aditiya; Nuswantoro, Ari; Triana, Linda; Ihsan, Bagus Muhammad
Jurnal Ners Vol. 8 No. 1 (2024): APRIL 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v8i1.22203

Abstract

characterised by high blood sugar levels as well as problems with protein, lipid, and carbohydrate metabolism as a result of inadequate insulin activity. Patients who have wounds infected by pathogenic bacteria, both Gram positive and Gram negative, run the risk of developing a number of problems, including neuropathy, hypertension, coronary heart disease, retinopathy, nephropathy, and gangrene. This cross-sectional, observational, descriptive study aims to pinpoint pathogenic bacteria in the diabetic patients' wounds at the diabetes wound care clinic in Pontianak City. The bacterial identification stage includes inoculation on enrichment media, Gram staining, inoculation and observation of colonies on differential-selective media, and biochemical tests. Examination results showed that Klebsiella pneumoniae was identified in 20 samples (57%), Pseudomonas aeruginosa in 8 samples (23%), and Staphylococcus aureus in 7 samples (20%). According to the study, diabetic wound problems put DM patients at risk of contracting dangerous microorganisms and spreading them to others. In order to prevent infection and other health issues, patients are instructed on how to properly care for their wounds. They are also provided the correct follow-up by medical professionals.
The potential of Cilembu sweet potato (Ipomoea batatas L.) as a growth medium for Staphylococcus aureus and Escherichia coli Nuswantoro, Ari; Rahayu, Merda Sri; Fatayati, Imma; Syopingi, Syopingi; Maretalinia, Maretalinia
JURNAL INFO KESEHATAN Vol 22 No 1 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss1.1087

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The media aims to store, reproduce, and identify bacteria but has disadvantages such as being expensive, containing chemicals, and being easily damaged due to contamination. Therefore we need an alternative media that can overcome these limitations. Cilembu sweet potato (Ipomoea batatas, L.) is a natural ingredient that is easy to obtain and contains sufficient nutrients so it has the potential to be used as a basic ingredient for growth media. The purpose of this study was to determine the potential of Cilembu sweet potato flour as an alternative medium for the growth of Staphylococcus aureus and Escherichia coli bacteria with a quasi-experimental design. Cilembu sweet potato flour is obtained by cleaning, chopping, drying in the oven, crushing with a blender, and sifting the tubers to obtain fairly fine flour. The flour was dissolved in agar and then inoculated with S. aureus and E. coli, each with 16 replications. The results showed that the average number of S. aureus colonies was 119.12 CFU (169.2 CFU in control) while E. coli was 160.56 CFU (221.2 CFU in control). The Mann-Whitney test showed that there was a difference in the number of S. aureus colonies on alternative media and NA (p = 0.006 ≤ 0.05), but there was no difference between the number of E. coli colonies on alternative media and NA (p = 0.057 > 0.05). Finally, there was a difference in the number of S. aureus and E. coli colonies on alternative media (p = 0.04 ≤ 0.05). The nutritional composition shows that Cilembu sweet potato flour has more potential to replace NA as a growth medium for E. coli than for S. aureus.
Normal dan Dislipidemia: Profil Lipid Pasien Hipertensi- Prolanis di Kota Pontianak Sen, Sharen; Nuswantoro, Ari; Kamilla, Laila; Aprilia, Dinasti
MAHESA : Malahayati Health Student Journal Vol 4, No 1 (2024): Volume 4 Nomor 1 (2024)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v4i1.13266

Abstract

ABSTRACT The primary cause of cardiovascular disease (CVD) is hypertension (HT). Through the Prolanis program, BPJS treats HT patients in Indonesia through various activities, including physical activity and monitoring lipid profiles, which until now have not been widely reported. The aim of this study was to describe the lipid profile of HT patients participating in Prolanis in Pontianak City. The samples are the medical records from 110 patients chosen using the total sampling method in 2022 at the Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak. Information gathered from 99 women and 11 men revealed that 80.8% of the women had hypercholesterolemia; triglycerides and HDL were mainly within normal ranges; LDL ranged from normal (13.6%) to extremely high (8.2%); and the ratios of cholesterol to HDL and triglycerides to HDL were mainly normal. One of the Prolanis program's goals, physical exercise, appears to have an impact on the patient's typical lipid profile. Nonetheless, the fact that some people have dyslipidemia raises awareness of the risk of CVD and supports the necessity of keeping this program in place. Keywords: Cardiovascular Disease, Lipid Profile, Hypertensive Patients, Prolanis  ABSTRAK Penyebab utama penyakit kardiovaskular (CVD) adalah hipertensi (HT). Melalui program Prolanis, BPJS menangani pasien HT di Indonesia melalui berbagai kegiatan, termasuk aktivitas fisik dan pemantauan profil lipid, yang hingga saat ini belum banyak dilaporkan. Tujuan dari penelitian ini adalah untuk mendeskripsikan profil lipid pasien HT yang mengikuti Prolanis di Kota Pontianak. Sampel penelitian ini adalah rekam medis dari 110 pasien yang dipilih dengan menggunakan metode total sampling pada tahun 2022 di Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak. Informasi yang dikumpulkan dari 99 wanita dan 11 pria menunjukkan bahwa 80,8% wanita mengalami hiperkolesterolemia; trigliserida dan HDL sebagian besar dalam kisaran normal; LDL berkisar dari normal (13,6%) hingga sangat tinggi (8,2%); dan rasio kolesterol terhadap HDL serta trigliserida terhadap HDL sebagian besar dalam kisaran normal. Salah satu tujuan program Prolanis, yaitu latihan fisik, tampaknya berdampak pada profil lipid pasien. Meskipun demikian, fakta bahwa beberapa orang memiliki dislipidemia meningkatkan kesadaran akan risiko CVD dan mendukung perlunya mempertahankan program ini. Kata Kunci: Penyakit Kardiovaskular, Profil Lipid, Pasien Hipertensi, Prolanis
IDENTIFIKASI DAN PROFIL RESISTENSI BAKTERI PADA MINUMAN AIR TAHU DAN AIR TEBU DI WILAYAH KOTA PONTIANAK Aditia, Aditia; Nuswantoro, Ari; Djohan, Herlinda; Ihsan, Bagus Muhammad; Uq'ma, Nurul; Shafitri, Anisya Nur; Affifatuzahara, Affifatuzahara; Al-qorifah, Fahma Ajeng; Anjelica, Benedikta
Jurnal Ilmiah Global Education Vol. 4 No. 4 (2023): JURNAL ILMIAH GLOBAL EDUCATION, Volume 4 Nomor 4, Desember 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/jige.v4i4.1860

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Tofu and sugarcane juice drinks are in great demand because they taste delicious and affordable. These drinks can be contaminated with pathogenic bacteria due to hygiene and poor environment. Contamination of pathogenic bacteria that are resistant to antibiotics is a serious health threat.  The objective of this study is to identify and provide antibiotic resistance bacteria from tofu and sugarcane juice drinks overview. This Study is a descriptive-observation with a cross-sectional design. Bacterial identification is carried out by culture, while resistance testing is carried out by Kirby-Bauer. Penicillin, amoxicillin, ampicillin, ciprofloxacin, chloramphenicol, gentamicin, tetracycline, and vancomycin were used. 43 samples of tofu water drink and 70 samples of sugarcane juice drink were selected by purposive sampling. Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes, and Klebsiella pneumoniae founds in tofu drinks, while Enterobacter aerogenes, Salmonella typhi, Shigella dysenteriae, and Escherichia coli founds in sugarcane juice. Staphylococcus aureus resistant to penicillin (100%), Salmonella typhi resistant to penicillin (66.6%), Enterobacter aerogenes resistant to tetracycline (70%), Klebsiella pneumoniae resistant to penicillin (100%), Shigella dysenteriae resistant to ampicillin and gentamicin (100%), and Escherichia coli resistant to vancomycin (100%). Pathogenic-resistant bacteria in tofu and sugarcane juice drinks show the risk of spreading resistant bacteria which can trigger bacterial resistance with a wider spectrum.
PROFIL LIPID SEBAGAI PREDIKTOR UREUM DAN KREATININ PADA PASIEN PROLANIS PENDERITA DMT2 DI UPT PUSAT LABKES KOTA PONTIANAK Pratiwi, Tarisya Fitri; Fatayati, Imma; Supriyanto, Supriyanto; Nuswantoro, Ari; Aprillia, Dinasti
Jurnal Review Pendidikan dan Pengajaran Vol. 7 No. 4 (2024): Vol. 7 No. 4 Tahun 2024
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v7i4.33968

Abstract

Lebih dari 1 miliar orang diperkirakan akan menderita diabetes melitus (DM) pada tahun 2050. Keadaan dislipidemia akibat DM menaikkan risiko inisiasi komplikasi nefropati diabetik. Dislipidemia memiliki dampak tidak langsung terhadap penurunan fungsi ginjal karena dapat menyebabkan aterosklerosis, yang membuat ginjal bekerja lebih keras untuk menyaring darah dari waktu ke waktu. Tujuan penelitian ini adalah untuk mengetahui apakah profil lipid berhubungan dengan ureum dan kreatinin sebagai parameter fungsi ginjal pada pasien prolanis penderita DMT 2 yang terdaftar di Unit Pelaksana Teknis (UPT) Pusat Laboratorium Kesehatan Kota Pontianak. Penelitian ini berbentuk deskriptif dan berdesain cross-sectional dengan menggunakan data sekunder rekam medis pasien periode Januari 2023 sampai dengan Desember 2023 pasien prolanis penderita DMT 2 yang terdaftar di UPT Pusat Laboratorium Kesehatan Kota Pontianak yang dipilih menggunakan teknik purposive sampling, dengan kriteria yang diberlakukan yaitu pasien prolanis penderita DMT 2 yang melakukan pemeriksaan kolesterol total, trigliserida, HDL, LDL, ureum dan kreatinin dan terdaftar di UPT Pusat Laboratorium Kesehatan Kota Pontianak sehingga didapatkan 339 responden. Analisis statistik dengan uji Kendall’s Tau-b menunjukkan kadar HDL berhubungan dengan kadar ureum dan kadar kreatinin dengan nilai signifikansi berturut-turut adalah 0,035 (p <0,05) dan 0,001 (p < 0,01). Serta didapatkan nilai koefisien korelasi berturut-turut adalah r = -0,078 dan -0,123 yang artinya semakin rendah kadar HDL maka semakin tinggi kadar ureum dan kadar kreatinin pasien tersebut.
The Relationship Between Premature Rupture of Membranes (PROM) and Leukocyte Levels in Newborns Grovindo Grovindo; Sutriswanto Sutriswanto; Ari Nuswantoro; Panyada Cholsakhon
MEDICA (International Medical Scientific Journal) Vol. 7 No. 1 (2025): MEDICA (International Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i1.484

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Premature Rupture of Membranes (PROM) remains a global concern in obstetrics due to its potential to cause complications such as infections that increase maternal and neonatal morbidity and mortality. PROM occurs in approximately 10–15% of pregnancies worldwide and 8–10% in Indonesia, often before 37 weeks of gestation, contributing significantly to preterm births and neonatal deaths. Risk factors for PROM include maternal age, parity, infection, and other pregnancy-related conditions. Infection resulting from PROM can be identified through leukocyte count in newborns, with leukocytosis indicating the presence of infection. This study aimed to examine the relationship between PROM and leukocyte levels in newborns at RSUD Drs. Jacobus Luna, M.Si, Bengkayang Regency, using a descriptive-analytic design with a cross-sectional approach and total sampling of 88 respondents. Results showed that among 36 newborns with elevated leukocyte levels, 28 cases (77.8%) were born from PROM pregnancies, whereas only 8 cases (22.2%) were non-PROM. Among 52 cases with normal leukocyte levels, only 11 newborns (21.2%) had PROM, while 41 (78.8%) did not. These findings indicate that newborns from PROM pregnancies tend to have higher leukocyte counts. Statistical analysis using Chi-square showed a significant relationship between PROM and leukocyte levels, with a p-value of 0.000 (< 0.05). In conclusion, PROM is a notable risk factor for increased leukocyte counts in neonates, underscoring the importance of early detection and management of PROM to reduce the risk of neonatal infection and its associated complications.
The Antibacterial Effectiveness of Moringa Oleifera Leaves Extract Against the Growth of Escherichia coli Using the Diffusion Method Liza Harum Nur Annisa; Linda Triana; Emilda Sari; Ari Nuswantoro
MEDICA (International Medical Scientific Journal) Vol. 6 No. 3 (2024): MEDICA (International Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i3.487

Abstract

Moringa oleifera leaves a common herbal plant found in tropical regions and naturalized in Indonesia, are known to be rich in bioactive compounds such as flavonoids, tannins, terpenoids, alkaloids, and saponins. These compounds have potential medicinal properties, including antibacterial activity. This study aims to examine the antibacterial effectiveness of moringa leaf extract against the growth of Escherichia coli using the diffusion method. This descriptive-analytical research employed a purposive sampling technique with varying concentrations of moringa leaf extract: 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. Each concentration was tested in triplicate, resulting in a total of 30 samples. The antibacterial effectiveness test was conducted using the agar diffusion method with blank paper disks. The results showed that no inhibition zones were formed at concentrations of 10% and 20%. Inhibition zones began to appear at 30% concentration (7.3 mm), increasing with higher concentrations: 40% (9 mm), 50% (10.3 mm), 60% (11.3 mm), 70% (16.6 mm), 80% (23.3 mm), 90% (28.3 mm), and reaching 34.6 mm at 100% concentration. Based on the antibacterial activity category, 10% and 20% showed no activity, 30% and 40% were categorized as weak, 50% and 60% as moderate, 70% as strong, and 80%, 90%, and 100% as very strong. It can be concluded that an 80% concentration of moringa leaf extract is the most effective in inhibiting the growth of Escherichia coli.
The Effect of Different Soaking Times of Gambir (Uncaria gambir (Hunter) Roxb.) on the Examination of Ascaris lumbricoides Eggs as an Alternative Stain to Eosin Tantri Rahmatika; Ari Nuswantoro; Laila Kamilla
MEDICA (International Medical Scientific Journal) Vol. 7 No. 2 (2025): MEDICA (International Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i2.512

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Ascaris lumbricoides is a type of helminth that can infect humans. One laboratory method for its examination is the use of 2% eosin staining with the direct slide method. However, eosin poses potential hazards to both the environment and human health, thus prompting the need for an alternative, eco-friendly staining agent. Gambir (Uncaria gambir (Hunter) Roxb.) contains red catechu, which imparts a red coloration to solutions. This study aims to evaluate the staining differences based on varying soaking times of Gambir extract for staining Ascaris lumbricoides eggs as a natural alternative to eosin. This research is a quasi-experimental study using purposive sampling. The Gambir samples were soaked in 96% ethanol for 1 hour, 1 hour 30 minutes, and 2 hours. Each treatment was replicated 9 times, resulting in a total of 27 samples. The staining was conducted using the direct slide method to examine Ascaris lumbricoides eggs. The results showed that Gambir soaked for 1 hour yielded a staining quality of 41.67%, 1 hour 30 minutes resulted in 68.51%, and 2 hours resulted in 87.96%, while the 2% eosin control achieved a staining quality of 100%. Based on the results of this study on the variation in soaking time of gambier (Uncaria gambir (Hunter) Roxb.) as an alternative dye to eosin in the examination of Ascaris lumbricoides eggs, it can be concluded that the staining success rate increases with longer soaking durations.
Number of Bacterial Colonies After Washing Hands with Antiseptic Soap and Regular Soap: A Study on Department of Health Analyst Students in Poltekkes Kemenkes Pontianak Ari Nuswantoro; Maulidiyah Salim; Slamet; Dinasti Aprillia
Jurnal teknologi Kesehatan Borneo Vol 1 No 2 (2020): Jurnal Teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v1i2.27

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Hand washing is a healthy activity carried out by many parties. Getting used to wash your hands properly will increase health. The soap on the market is plain hand washing soap and antiseptic handwashing soap. The second type is claimed to be more effective in reducing the amount of bacterial contamination than plain soap because it contains antimicrobial ingredients to increase product sales. This study aims to determine the differences in the number of bacterial colonies on the palms of students who wash their hands with antiseptic soap and plain soap among students of Analyst Department of Pontianak Health Polytechnic, Minister of Health. This research was quasi-experimental, with 24 respondents. Each respondent was asked to wash hands properly twice, the first with plain soap that does not contain an antiseptic (A) and the second with soap containing antiseptic (B). After examining the number of bacteria on the respondent’s thumb, the average number of bacterial colonies got in treatments A and B was 45,5 and 38,8 CFU, respectively. Mann-Whitney U statistical test results got a probability value of 0.391> 0.05, which means there is no difference in the number of bacterial colonies in the palms that washing hands with plain soap and with antiseptic soap. This study revealed the fact that the number of bacterial colonies in the group that washed hands with antiseptic soap was lower than those who washed their hands with ordinary soap.
Co-Authors Adawiyah, Rabi’atul Aditia Aditia Aditia, Aditia Affifatuzahara Affifatuzahara Affifatuzahara, Affifatuzahara Al-qorifah, Fahma Ajeng Alfianita, Riska Anisya Nur Shafitri Anjelica, Benedikta Aprilia, Dinasti Ardhani, Adha Riesta Arifah, Rizki Zanuba Artanti, Lina Yunda Astuti, Fitri Mega Aufani, Destia Aulia, Annisa Syafa Azura, Azura Azzahra, Larasabella Bagus Muhammad Ihsan Benedikta Anjelica Berlianti, Jessica Ningtyas Desi Wahyumarniasari Devi Nurfitri Bintang Dinasti Aprillia Djohan, Herlinda Edi Santoso Emilda Sari Fadilla, Alda Nur Fahma Ajeng Al-qorifah Fahrani, Chantika Lailatussa'adah Fitra Fathuhudin Grovindo Grovindo Hartati, Fitri Ulya Hendra Budi Sungkawa, Hendra Budi Herlinda Djohan Ihsan, Bagus Muhammad Imma Fatayati Indah Purwaningsih Indah Purwaningsih Indrawati, Ratih Jordan, Aristo Juliana Cristyaningsih Kartini Kartini Laila Kamilla Laila Kamilla Linda Triana Linda Triana Liza Harum Nur Annisa Maretalinia Maretalinia Maretalinia, Maretalinia Maulidiyah Salim Merda Sri Rahayu Monalita, Ria Muhammad Ihsan, Bagus Munardi, Fhatia Natasya Natasya Intan Ramadhani Natasya Intan Ramadhani Nurhayati, Etiek Nurul Uq'ma P Purwanto Panyada Cholsakhon Perdede, Lusi Hernawati Pratiwi, Tarisya Fitri Rahayu, Merda Sri Ramadan, Aditiya Ratnawaty, Gervacia Jenny Ratulangi, Agus Dimas Reza Setiawan Reza, Nur Rezky, Muhammad Andyka Riska Alfianita Rizki Zanuba Arifah Rizki, Sri Yuniarti Romadi Romadi Sadid, Randy Samwidya, Veronika Sriayu Sen, Sharen Shafitri, Anisya Nur Slamet Slamet Sri Tumpuk Sugito Sugito Sugito Sugito Sutriswanto Sutriswanto Suwandi, Edy Syahdilla, Ade Syopingi Syopingi Syopingi, Syopingi Tantri Rahmatika Theresia Indah Budhy Sulisetyawati Tika, Dian Tumpuk, Sri Uq'ma, Nurul Veny Veny Wahdaniah Wahyudi Wicaksono, Didik Yunus, Mohd