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All Journal Journal Of Vocational Health Studies Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Riset Kesehatan Poltekkes Depkes Bandung INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Ners Jurnal teknologi Kesehatan Borneo MAHESA : Malahayati Health Student Journal Jurnal EduHealth JURNAL ILMIAH GLOBAL EDUCATION Innovative: Journal Of Social Science Research Jurnal Vokasi Kesehatan Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Media Penelitian dan Pengembangan Kesehatan MEDICA (International Medical Scientific Journal) Jurnal Info Kesehatan ELECTRON (Journal of Science and Technology)
Ari Nuswantoro
Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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The Effectiveness of Chocolate in Reducing the Number of Methicillin-Resistant Staphylococcus aureus Colonies in Rattus norvegicus Edy Suwandi; Ari Nuswantoro; Sugito Sugito; Desi Wahyumarniasari; Muhammad Reza Setiawan; Dinasti Aprillia; Devi Nurfitri Bintang
JURNAL INFO KESEHATAN Vol 19 No 2 (2021): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol19.Iss2.545

Abstract

Chocolate has long been understood to provide positive emotions and a good mood if consumed in moderation. Chocolate contains prebiotics naturally from its constituent ingredients produced during the production process. Prebiotics, frequently oligosaccharides, are substances which cannot be metabolized by the human digestive system but can be employed by a group of bacteria in the gut, understood as probiotics. The positive relationship among them provides benefits for the host in eliminating pathogens. One of the well-known pathogens which frequently cause infection either in the community or in hospitals is methicillin-resistant Staphylococcus aureus (MRSA). Since it was first identified in 1960, MRSA has caused health problems until today. Research conducted on two groups of Rattus norvegicus infected with MRSA and then fed chocolate revealed a decrease in the average number of bacterial colonies on the skin compared to the control group. In the group fed chocolate at a dose of 50 mg/day, the bacterial colonies decreased to 1.28 x 108 CFU/cm2 in 7 days, lower than in the control group (1.46 x 108 CFU/cm2) at the same time. While those fed 75 mg/day chocolate decreased to 2.70 x 107 CFU/cm2 and the three groups were significantly different (0.000<0.05). Prebiotics fermented by probiotics release short-chain fatty acids (SCFA), which compete with the pathogens for attaching to the epithelial wall so that pathogens lose space and nutrients to survive. However, the adverse effect of chocolate may occur because it contains sugar which is a nutrient for bacteria, but if the balance of normal flora and adequate intake of prebiotics are administered, the pathogen could be eliminated.
APPLICATION OF MUSA BALBISIANA LOTION AGAINST STAPHYLOCOCCUS AUREUS Rezky, Muhammad Andyka; Budhy, Theresia Indah; Nuswantoro, Ari; Tika, Dian; Adawiyah, Rabi’atul
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 17 No 1 (2025): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v17i1.2818

Abstract

Staphylococcus aureus merupakan bakteri patogen yang dapat menyebabkan berbagai infeksi, terutama pada kulit, seperti bisul, impetigo, dan abses. Infeksi ini sering kali ditandai dengan peradangan, nekrosis, dan pembentukan nanah, yang secara signifikan dapat mengganggu fungsi kulit sebagai pelindung tubuh. Tantangan utama dalam pengelolaannya adalah meningkatnya resistensi antibiotik, termasuk Methicillin-Resistant Staphylococcus aureus (MRSA), sehingga diperlukan alternatif yang efektif, seperti bahan herbal, untuk mengatasi permasalahan ini. Kulit pisang kepok kuning yang sering berakhir sebagai limbah organik, diketahui mengandung senyawa aktif seperti flavonoid, alkaloid, saponin, tanin, dan antioksidan. Senyawa-senyawa tersebut memiliki aktivitas antibakteri yang signifikan, disertai dengan kemampuan melindungi kulit dari kerusakan akibat radikal bebas. Penelitian ini bertujuan untuk menjelaskan perbedaan efektivitas losion berbahan dasar ekstrak kulit pisang kepok kuning dengan tiga konsentrasi berbeda, yaitu formula I (30%), formula II (60%), dan formula III (90%) dalam menghambat pertumbuhan Staphylococcus aureus. Penelitian berbentuk quasi eksperimental desain ini menggunakan metode difusi cakram. Hasil penelitian menunjukkan bahwa rata-rata diameter zona hambat formula I adalah 5,60 mm, formula II adalah 8,30 mm, dan formula III adalah 8,70 mm. Analisis statistik dengan uji Kruskal-Wallis menunjukkan nilai p = 0,004, yang mengindikasikan adanya perbedaan signifikan antar formula. Kesimpulannya, losion ekstrak kulit pisang kepok kuning menunjukkan efektivitas yang signifikan dalam menghambat pertumbuhan Staphylococcus aureus. Efektivitas ini meningkat secara proporsional dengan konsentrasi ekstrak, sehingga produk ini memiliki potensi untuk digunakan sebagai losion antibakteri.
Relationship between duration of treatment and urine protein in pulmonary tuberculosis patients Sadid, Randy; Salim, Maulidiyah; Nuswantoro, Ari; Yunus, Mohd
ELECTRON (Journal of Science and Technology) Vol. 7 No. 1 (2025): ELECTRON (Journal of Science and Technology)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/electron.v7i1.521

Abstract

Tuberculosis is an infectious disease caused by the bacterium Mycobacterium tuberculosis. TB treatment is an efficient way to prevent the spread of the bacteria. The goals of TB treatment include curing, maintaining the patient's quality of life and productivity, preventing death from active TB or its sequelae, preventing relapse, reducing transmission to others, and preventing drug resistance. Most TB patients complete treatment without significant side effects, but a minority experience significant side effects. The duration of drug consumption can affect other organs such as the kidneys. Proteinuria is a condition in which there is protein in the urine beyond normal limits. The purpose of this study was to determine the relationship of length of treatment to urine protein in TB patients in Meliau District. This research method is analytical observational with a cross-sectional approach and consecutive sampling technique, with a total sample of 47 respondents. The results showed 10 respondents (21.27%) experienced proteinuria, while 37 respondents (78.73%) did not. Chi-Square analysis showed a relationship between length of treatment and urine protein in TB patients in Meliau District, as evidenced by a p-value of 0.015 (<0.05). In conclusion, there is a relationship between length of treatment and urine protein in TB patients in Meliau District.
The Relationship Between Premature Rupture of Membranes (PROM) and Leukocyte Levels in Newborns Grovindo, Grovindo; Sutriswanto, Sutriswanto; Nuswantoro, Ari; Cholsakhon, Panyada
MEDICA (International Medical Scientific Journal) Vol. 7 No. 1 (2025): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i1.484

Abstract

Premature Rupture of Membranes (PROM) remains a global concern in obstetrics due to its potential to cause complications such as infections that increase maternal and neonatal morbidity and mortality. PROM occurs in approximately 10–15% of pregnancies worldwide and 8–10% in Indonesia, often before 37 weeks of gestation, contributing significantly to preterm births and neonatal deaths. Risk factors for PROM include maternal age, parity, infection, and other pregnancy-related conditions. Infection resulting from PROM can be identified through leukocyte count in newborns, with leukocytosis indicating the presence of infection. This study aimed to examine the relationship between PROM and leukocyte levels in newborns at RSUD Drs. Jacobus Luna, M.Si, Bengkayang Regency, using a descriptive-analytic design with a cross-sectional approach and total sampling of 88 respondents. Results showed that among 36 newborns with elevated leukocyte levels, 28 cases (77.8%) were born from PROM pregnancies, whereas only 8 cases (22.2%) were non-PROM. Among 52 cases with normal leukocyte levels, only 11 newborns (21.2%) had PROM, while 41 (78.8%) did not. These findings indicate that newborns from PROM pregnancies tend to have higher leukocyte counts. Statistical analysis using Chi-square showed a significant relationship between PROM and leukocyte levels, with a p-value of 0.000 (< 0.05). In conclusion, PROM is a notable risk factor for increased leukocyte counts in neonates, underscoring the importance of early detection and management of PROM to reduce the risk of neonatal infection and its associated complications.
The Antibacterial Effectiveness of Moringa Oleifera Leaves Extract Against the Growth of Escherichia coli Using the Diffusion Method Annisa, Liza Harum Nur; Triana, Linda; Sari, Emilda; Nuswantoro, Ari
MEDICA (International Medical Scientific Journal) Vol. 6 No. 3 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i3.487

Abstract

Moringa oleifera leaves a common herbal plant found in tropical regions and naturalized in Indonesia, are known to be rich in bioactive compounds such as flavonoids, tannins, terpenoids, alkaloids, and saponins. These compounds have potential medicinal properties, including antibacterial activity. This study aims to examine the antibacterial effectiveness of moringa leaf extract against the growth of Escherichia coli using the diffusion method. This descriptive-analytical research employed a purposive sampling technique with varying concentrations of moringa leaf extract: 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. Each concentration was tested in triplicate, resulting in a total of 30 samples. The antibacterial effectiveness test was conducted using the agar diffusion method with blank paper disks. The results showed that no inhibition zones were formed at concentrations of 10% and 20%. Inhibition zones began to appear at 30% concentration (7.3 mm), increasing with higher concentrations: 40% (9 mm), 50% (10.3 mm), 60% (11.3 mm), 70% (16.6 mm), 80% (23.3 mm), 90% (28.3 mm), and reaching 34.6 mm at 100% concentration. Based on the antibacterial activity category, 10% and 20% showed no activity, 30% and 40% were categorized as weak, 50% and 60% as moderate, 70% as strong, and 80%, 90%, and 100% as very strong. It can be concluded that an 80% concentration of moringa leaf extract is the most effective in inhibiting the growth of Escherichia coli.
The Effect of Different Soaking Times of Gambir (Uncaria gambir (Hunter) Roxb.) on the Examination of Ascaris lumbricoides Eggs as an Alternative Stain to Eosin Rahmatika, Tantri; Nuswantoro, Ari; Kamilla, Laila
MEDICA (International Medical Scientific Journal) Vol. 7 No. 2 (2025): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i2.512

Abstract

Ascaris lumbricoides is a type of helminth that can infect humans. One laboratory method for its examination is the use of 2% eosin staining with the direct slide method. However, eosin poses potential hazards to both the environment and human health, thus prompting the need for an alternative, eco-friendly staining agent. Gambir (Uncaria gambir (Hunter) Roxb.) contains red catechu, which imparts a red coloration to solutions. This study aims to evaluate the staining differences based on varying soaking times of Gambir extract for staining Ascaris lumbricoides eggs as a natural alternative to eosin. This research is a quasi-experimental study using purposive sampling. The Gambir samples were soaked in 96% ethanol for 1 hour, 1 hour 30 minutes, and 2 hours. Each treatment was replicated 9 times, resulting in a total of 27 samples. The staining was conducted using the direct slide method to examine Ascaris lumbricoides eggs. The results showed that Gambir soaked for 1 hour yielded a staining quality of 41.67%, 1 hour 30 minutes resulted in 68.51%, and 2 hours resulted in 87.96%, while the 2% eosin control achieved a staining quality of 100%. Based on the results of this study on the variation in soaking time of gambier (Uncaria gambir (Hunter) Roxb.) as an alternative dye to eosin in the examination of Ascaris lumbricoides eggs, it can be concluded that the staining success rate increases with longer soaking durations.
Resistance Profile Antibiotics Pathogenic Bacteria from SWAB Wounds of Pontianak City Diabetes Treatment Clinic Patients azura, Azura; Sri Tumpuk; Ari Nuswantoro
Jurnal EduHealth Vol. 14 No. 04 (2023): Jurnal eduHealt, 2023, December
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes mellitus (DM) is a type of metabolic disorder where the sufferer has high blood sugar levels due to the body's failure to respond or produces insufficient amounts of insulin, or a metabolic disorder caused by hyperglycemia due to abnormalities in insulin secretion and insulin action or both. One of the complications of diabetes is ulcers, where a superficial infection occurs on the sufferer's skin and becomes a strategic location for bacterial growth. Most of the drugs that are widely used to inhibit or kill bacteria that cause infections in humans are antibiotics which carry the risk of drug resistance. Antibiotic resistance is a growing problem in many parts of the world. Antibiotic resistance can occur due to inappropriate or excessive use of antibiotics. This study aims to determine the susceptibility of pathogenic bacteria to several antimicrobial agents or antibiotics and to determine the percentage of antibiotic resistance in diabetes mellitus patients who experience complications, which will make it easier to choose the right antibiotic for healing. This research uses a descriptive method using samples of bacterial isolates which have been identified as 11 samples of Staphylococcus aureus bacterial isolates, 11 samples of Pseudomonas aeruginosa isolates and 39 samples of Klebsiella pneumoniae bacterial isolates. Antimicrobial susceptibility test (AST) disk diffusion method (Kirby & Bauer test) using Mueller Hinton media. With eight different types of antibiotics, this test was carried out to prove antimicrobial activity by measuring the diameter of the antimicrobial activity inhibition zone. Resistance test results were obtained in Staphylococcus aureus experiencing the highest resistance to the antibiotic Gentamycin (CN) at 63.63%, in Pseudomonas aeruginosa experiencing high resistance to the antibiotic Ampicillin (AMP) at 54.54%, and in Klebsiella pneumoniae experiencing the highest resistance. against the antibiotic Ampicillin (AMP) was 41.02%. Preventing antibiotic resistance in wound healing is very important to ensure antibiotics remain effective in treating infections. Therefore, this research was carried out in order to determine the correct type of antibiotic for healing wounds in diabetes mellitus sufferers.
Potensi Air Perasan Batang Bayam Merah (Amaranthus Tricolor L) Sebagai Alternatif Pewarnaan Telur Cacing Soil Transmitted Helminth Artanti, Lina Yunda; Sungkawa, Hendra Budi; Djohan, Herlinda; Nuswantoro, Ari; Alfianita, Riska
Innovative: Journal Of Social Science Research Vol. 4 No. 4 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i4.13043

Abstract

in the examination of worm eggs uses 2% eosin. However, eosin has shortcomings because it is not easily decomposed, so an alternative coloring that is more environmentally friendly is needed. Red spinach plants have the potential to be used as a natural dye because they contain anthocyanin compounds. This study aims to determine the potential of red spinach stem juice (amaranthus tricolor L) as an alternative to staining soil transmitted helminth eggs. Research design of quasi experiment with purposive sampling technique. Samples used red spinach stem juice (amaranthus tricolor L) in a ratio of 1:1, 1:2, 1:3, 1:4, and 1:5 by direct method and repeated 5 times. Based on the results of the research, the juice of red spinach stems in the ratio of 1:1 showed 60% results, the ratio of 1:2 showed 53% results, the ratio of 1:3 showed 53% results, the ratio of 1:4 showed 40% results and the ratio of 1:5 showed 40% results. The conclusion of this study is that the concentration of 1: 1 has the highest quality which is considered to have potential as an alternative coloring.
Identifikasi Bakteri Coliform pada Minuman Air Tahu yang Dijual di Wilayah Kota Pontianak Rizki, Sri Yuniarti; Jordan, Aristo; Ardhani, Adha Riesta; Nurhayati, Etiek; Nuswantoro, Ari
Innovative: Journal Of Social Science Research Vol. 4 No. 4 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i4.14503

Abstract

Di Kota Pontianak, air tahu merupakan minuman yang populer di semua kalangan, baik anak-anak, remaja, bahkan orang dewasa. Rasanya enak dan menyegarkan. Minuman air tahu yang dipasarkan di Kota Pontianak ditemukan mengandung bakteri coliform yang sangat berbahaya bagi kesehatan. Penelitian ini dilakukan dengan menggunakan metode kultur untuk mengetahui apakah minuman air tahu yang dijual di pinggir jalan di Kota Pontianak terkontaminasi bakteri coliform. Penelitian ini menggunakan desain cross sectional dan berbentuk deskriptif dengan menggunakan teknik incidental sampling. Sebanyak 43 sampel air tahu yang dijual di pinggir jalan di Kota Pontianak digunakan dalam penelitian ini. Dari 43 sampel air tahu yang diperiksa, 17 (39,5%) sampel dinyatakan positif mengandung bakteri coliform, sedangkan 26 (60,5%) sampel lainnya dinyatakan negatif bakteri coliform.
Gambaran Kadar Glukosa Darah Sewaktu Pada Ibu Hamil di Puskesmas Tambelan Sampit Kota Pontianak Ramadan, Aditiya; Nuswantoro, Ari; Triana, Linda; Ihsan, Bagus Muhammad
Jurnal Ners Vol. 8 No. 1 (2024): APRIL 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v8i1.22203

Abstract

characterised by high blood sugar levels as well as problems with protein, lipid, and carbohydrate metabolism as a result of inadequate insulin activity. Patients who have wounds infected by pathogenic bacteria, both Gram positive and Gram negative, run the risk of developing a number of problems, including neuropathy, hypertension, coronary heart disease, retinopathy, nephropathy, and gangrene. This cross-sectional, observational, descriptive study aims to pinpoint pathogenic bacteria in the diabetic patients' wounds at the diabetes wound care clinic in Pontianak City. The bacterial identification stage includes inoculation on enrichment media, Gram staining, inoculation and observation of colonies on differential-selective media, and biochemical tests. Examination results showed that Klebsiella pneumoniae was identified in 20 samples (57%), Pseudomonas aeruginosa in 8 samples (23%), and Staphylococcus aureus in 7 samples (20%). According to the study, diabetic wound problems put DM patients at risk of contracting dangerous microorganisms and spreading them to others. In order to prevent infection and other health issues, patients are instructed on how to properly care for their wounds. They are also provided the correct follow-up by medical professionals.
Co-Authors Adawiyah, Rabi’atul Aditia Aditia Aditia, Aditia Affifatuzahara Affifatuzahara Affifatuzahara, Affifatuzahara Al-qorifah, Fahma Ajeng Alfianita, Riska Anisya Nur Shafitri Anjelica, Benedikta Annisa, Liza Harum Nur Aprilia, Dinasti Ardhani, Adha Riesta Arifah, Rizki Zanuba Artanti, Lina Yunda Astuti, Fitri Mega Aufani, Destia Aulia, Annisa Syafa Azura, Azura Azzahra, Larasabella Bagus Muhammad Ihsan Benedikta Anjelica Cholsakhon, Panyada Desi Wahyumarniasari Devi Nurfitri Bintang Dinasti Aprillia Djohan, Herlinda Edi Santoso Fadilla, Alda Nur Fahma Ajeng Al-qorifah Fahrani, Chantika Lailatussa'adah Fitra Fathuhudin Grovindo, Grovindo Hartati, Fitri Ulya Hendra Budi Sungkawa, Hendra Budi Herlinda Djohan Ihsan, Bagus Muhammad Imma Fatayati Indah Purwaningsih Indah Purwaningsih Indrawati, Ratih Jessica Ningtyas Berlianti Jordan, Aristo Juliana Cristyaningsih Kartini Kartini Laila Kamilla Linda Triana Maretalinia Maretalinia Maretalinia, Maretalinia Maulidiyah Salim Merda Sri Rahayu Monalita, Ria Muhammad Ihsan, Bagus Munardi, Fhatia Natasya Natasya Intan Ramadhani Nurhayati, Etiek Nurul Uq'ma P Purwanto Perdede, Lusi Hernawati Pratiwi, Tarisya Fitri Rahayu, Merda Sri Rahmatika, Tantri Ramadan, Aditiya Ratnawaty, Gervacia Jenny Reza Setiawan Reza, Nur Rezky, Muhammad Andyka Riska Alfianita Rizki Zanuba Arifah Rizki, Sri Yuniarti Romadi Romadi Sadid, Randy Samwidya, Veronika Sriayu Sari, Emilda Sen, Sharen Shafitri, Anisya Nur Slamet Sri Tumpuk Sugito Sugito Sugito Sugito Supriyanto Supriyanto Sutriswanto, Sutriswanto Suwandi, Edy Syahdilla, Ade Syopingi Syopingi Syopingi, Syopingi Theresia Indah Budhy Sulisetyawati Tika, Dian Tumpuk, Sri Uq'ma, Nurul Veny Veny Wahdaniah Wahyudi Wicaksono, Didik Yunus, Mohd