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Performan Ayam Broiler dengan Penambahan Tepung Daun dalam Pakan Ari Kusuma Wati; Zuprizal Zuprizal; Kustantinah Kustantinah; Edwin Indarto; Nanung Danar Dono; Wihandoyo Wihandoyo
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 16, No 2 (2018): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

The aim of this research was studied the effect of addition leaf meal in feed on performance of broiler chicken. The research used five groups of treatments, those were 0.0, 2.5, 5.0, 7.5, and 10.0% calliandra leaf meal on feed. Each treatment were consists of three replications. The data of feed intake, average daily gain, protein intake, energy intake and feed conversion ratio were taken every week for 4 weeks. The collected data were analyzed using Completely Randomized Design (CRD) and continued by tested using Duncan’s New Multiple Range Test (DMRT). The results showed that the addition of calliandra leaf meal significantly (P<0.05) on feed intake, body weight gain, protein intake, energy intake and feed conversion ratio. The result of this study can be concluded that utilization of calliandra leaf meal with maximum level 5.0% did not affect the performance of broiler.

MORFOLOGI USUS AYAM KAMPUNG UMUR 20 MINGGU YANG DISUPLEMENTASI DL-METIONIN DAN L-LISIN HCL DALAM PAKAN Charles V Lisnahan; Wihandoyo Wihandoyo; Zuprizal Zuprizal; Sri Harimurti
Journal of Tropical Animal Science and Technology Vol 1 No 1 (2019): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

The aim of this study was to determine the effect of the supplementation of dl-methionine and l-lysine HCl into feed based on cafeteria standards to intestinal morphology of native chickens at 20 weeks-old. A total of 128 fourteen week-old native chickens were used in this study. The native chickens were divided into 4 treatments groups with 4 replications. The dietary treatments were: T0 (feed based on cafeteria standard), T1 (feed based on protein standard of NRC), T2 (cafeteria feed+0.09% dl-methionine+0.22% l-lysine HCl), T3 (cafeteria feed+0.19% dl-methionine+0.42% l-lysine HCl). The data collected were villus height, crypt depth and villus width of duodenum, jejunum and ileum of native chickens aged 20 weeks. The result of this study for T0, T1, T2 and T3 showed that villus height of duodenum were 863.03; 1237.85; 1171.60 and 1261.15 µm, crypt depth 212.70; 192.08; 210.25 and 251.85 µm, villus width 168.74; 168.53; 213.61 and 188.60 µm. Villus height of jejunum were 1381.48; 1088.70; 1072.68 and 1383.15 µm, crypt depth 272.,05; 261.83; 225.00 and 205.65 µm, villus width 188.28; 166.41; 216.26 and 182.95 µm. villus height of ileum were 798.03; 863.35; 882.88 and 828.70 µm, crypt depth 199.58; 199.58; 154.68 and 145.78 µm and villus width 181.13; 154.55; 201.25 dan 175.65 µm, respectively. It can be concluded that supplementation of dl-methionine and l-lysine HCl was not effected upon villus height, crypt depth and villus width of duodenum, jejunum and ileum of native chickens aged 20 weeks.

Meat bone ratio (MBR) potongan komersial karkas ayam Broiler dengan nanoenkapsulasi ekstrak buah Mahkota Dewa Niati Ningsih; Taufik Ahmad Zulfian; Besse Mahbuba We Tenri Gading; Zuprizal
Jurnal Sains dan Teknologi Peternakan Vol 3 No 1 (2021): Jurnal Sains dan Teknologi Peternakan
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/jstp.v3i1.1403

The objective of this study was to determine the effect of supplementation nanoencapsulation of Mahkota Dewa fruit extract (NEMD) against the meat bone ratio (MBR) of the commercial part of the broiler chicken carcass. The research material was used 200 male broiler chickens aged eight days who were rearing in open house cages. The treatments consisted of drinking water without feed additives (P0; negative control), drinking water + tetracycline antibiotics (P1; positive control), drinking water + 2.5% Mahkota Dewa fruit extract (P2), drinking water + 2.5 % NEMD (P3), and drinking water + 5.0 % NEMD (P4). Parameters observed were slaughter weight, carcass weight, and MBR of the commercial part of the broiler chicken carcass. The data obtained were analyzed using a completely randomized design (CRD). Data with significant results were further tested using orthogonal contrast (P-Value < 0.05). The results showed that the use of NEMD up to 5% in drinking water did not have a significant effect on the MBR of the commercial part of the broiler chicken carcass. The 5% NEMD has not been able to work optimally to increase the MBR of the commercial part of the broiler chicken carcass.

Pengolahan Sinbiotik Kultur Campuran yang Berasal dari Kombinasi Bekatul Gandum sebagai Prebiotik dan Jus Kubis Terfermentasi sebagai Probiotik melalui Proses Fermentasi Cahya Setya Utama; Zuprizal Zuprizal; Chusnul Hanim; Wihandoyo Wihandoyo
Jurnal Aplikasi Teknologi Pangan Vol 9, No 3 (2020): Agustus 2020
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jatp.7442

Penelitian ini bertujuan untuk mengkaji kualitas sinbiotik kultur campuran yang berasal dari jus kubis terfermentasi sebagai probiotik yang ditambahkan pada bekatul gandum sebagai prebiotik melalui proses fermentasi. Penelitian ini dilakukan dengan menggunakan rancangan acak lengkap pola faktorial 3x3 dengan 3 ulangan. Faktor yang digunakan adalah lama pemeraman dan variasi konsentrasi jus kubis. Parameter yang diamati adalah komponen proksimat (kadar air, abu, lemak kasar, protein kasar, serat kasar dan bahan ekstrak tanpa nitrogen (BETN), komponen serat berupa acid detergent fibre atau ADF, neutral detergent fibre (NDF), selulosa, hemiselulosa dan lignin, serta gross energi, glukosa, sukrosa, mannosa, arabinosa, rafinosa, amilum, amilosa, amilopektin dan pati resisten serta profil sinbiotik kultur campuran melalui analisis scanning electron microscope (SEM). Hasil penelitian memperlihatkan adanya peningkatan yang signifikan pada kadar abu, protein kasar, serat kasar (p<0,05) sedangkan pada parameter BETN, ADF, NDF, hemiselulosa, lignin, gross energi, sukrosa, mannosa, arabinosa, rafinosa, amilosa, amilum, amilopektin dan pati resisten terdapat interaksi antar kedua faktor (p<0,05) namun pada parameter glukosa tidak terdapat interaksi antar kedua faktor. Kesimpulan penelitian yaitu sinbiotik kultur campuran terbaik terdapat pada penambahan 40% jus kubis terfermentasi dengan lama fermentasi 4 hari. Sinbiotik kultur campuran ini dapat digunakan sebagai sumber additive untuk pangan maupun kepentingan lainnya seperti pakan ternak.Processing of Mixed Culture Sinbiotics Originating from the Combination of Wheat Pollard as Prebiotics and Fermented Cabbage Juice as Probiotics through the Fermentation ProcessAbstractThe objective of this study was to examine the quality of mixed culture synbiotics derived from fermented cabbage juice as probiotics which were added to wheat pollard as a prebiotic through the fermentation process. The study used a completely randomized 3x3 factorial pattern design with 3 replications and the observed factors were duration of incubation and concentrations of applied cabbage juice. Proximate components (water content, ash, crude fat, crude protein, crude fiber and extraction material without nitrogen or BETN), fiber components (acid detergent fiber (ADF), neutral detergent fiber (NDF), cellulose, hemicellulose and lignin), gross energy, glucose, sucrose, mannose, arabinose, raffinose, starch, amylose, amylopectin and resistant starch and mix culture synbiotic profile through scanning electron microscope (SEM) were analyzed. The results showed a significant increase in ash content, crude protein, crude fiber (p<0.05) while in BETN, ADF, NDF parameters, hemicellulose, lignin, gross energy, sucrose, mannose, arabinose, raffinose, amylose, starch, amylopectin and resistant starch interaction between the two factors (p<0.05). However, in the glucose parameter there was no interaction between the two factors. The conclusion of the research is that the best mixed culture synbiotic is in the addition of 40% fermented cabbage juice with 4 days fermentation time. This mixed culture synbiotic can be used as an additive source for food and animal feed.

Pengaruh Lama Pemanasan terhadap Kualitas Kimia Wheat Pollard yang Berpotensi sebagai Prebiotik Cahya Setya Utama; Zuprizal Zuprizal Zuprizal; Chusnul Hanim; Wihandoyo Wihandoyo
Jurnal Aplikasi Teknologi Pangan Vol 8, No 3 (2019): Agustus 2019
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Penelitian bertujuan mengkaji pengaruh lama pemanasan wheat pollard dengan autoclave untuk mendapatkan monomer-monomer yang berpotensi sebagai prebiotik. Penelitian menggunakan rancangan acak lengkap pola searah dengan 4 perlakuan dan 4 ulangan. Parameter yang diamati adalah komponen proksimat (kadar air, abu, lemak kasar, protein kasar, serat kasar dan bahan ekstrak tanpa nitrogen atau BETN), komponen fiber (acid detergent fibre atau ADF, neutral detergent fibre atau NDF, selulosa, hemiselulosa, lignin), gross energi, mannosa, arabinosa, glukosa, sukrosa, rafinosa, amilosa, amilum, amilopektin, resistant starch dan profil wheat pollard melalui scanning electron microscope (SEM). Hasil penelitian menunjukkan bahwa terdapat pengaruh perlakuan yang nyata (p<0,05) terhadap kadar air, abu, protein kasar, serat kasar, BETN, NDF, hemiselulosa, lignin, selulosa, gross energi, rafinosa, glukosa, arabinosa, sukrosa, amilosa, amilum, amilopektin, resistant starch namun tidak ada pengaruh yang nyata (p>0,05) pada lemak kasar, ADF dan manosa. Indikator wheat pollard sebagai prebiotik terlihat dari peningkatan kadar rafinosa, arabinosa dan resistant starch berturut-turut sebesar 0,72 menjadi 3,95%; 0,51 menjadi 1,04%; 0,51 menjadi 1,04% dan 5,28 menjadi 14,15%. Kesimpulan penelitian ini adalah wheat pollard yang dipanaskan selama 15 menit dapat memberikan komposisi terbaik sebagai prebiotik.Effect of Heating Time on the Chemical Quality of Wheat Pollard as a Prebiotic Potential AgentAbstractThis study was done to examine the heating time of wheat pollard to obtain potentially prebiotic monomers. The study used a completely randomized design with 4 treatments and 4 replications. The observations were water content, ash, crude fat, crude protein, crude fiber, extract without nitrogen, acid detergent fiber or ADF, neutral detergent fiber or NDF, cellulose, hemicellulose, lignin, gross energy, mannose, arabinose, glucose, sucrose, raffinose, amylose, starch, amylopectin, resistant starch and wheat pollard profile through scanning electron microscope (SEM). The results showed that there was a significant treatment effect (p<0.05) on moisture, ash, crude protein, crude fiber, extract without nitrogen, NDF, hemicellulose, lignin, cellulose, gross energy, raffinose, glucose, arabinose, sucrose, amylose, starch, amylopectin, resistance starch but not significant effect (p>0.05) of treatments in crude fat, ADF and mannose. The indicator of wheat pollard as a prebiotic was able to be seen from an increase in raffinose, arabinose and resistance starch levels from 0.72 to 3.95%; 0.51 to 1.04%; 0.51 to 1.04%, and 5.28 to 14.15%, respectively. As conclusion, heating time for 15 minutes might provide a function to turn wheat pollard into prebiotic agent.

Isolasi dan Identifikasi Bakteri Asam Laktat Selulolitik yang Berasal dari Jus Kubis Terfermentasi Cahya Setya Utama; Zuprizal Zuprizal; Chusnul Hanim; Wihandoyo Wihandoyo
Jurnal Aplikasi Teknologi Pangan Vol 7, No 1 (2018): Februari 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Penelitian bertujuan mengisolasi dan identifikasi jenis bakteri asam laktat (BAL) selulolitik yang berasal dari jus kubis terfermentasi. Tahapan penelitian meliputi isolasi mikrobia dari jus kubis terfermentasi, karakteristik morfologi sel, karakteristik biokimiawi, identifikasi dengan kit api 50 CHL dan pengujian kemampuan mendegradasi selulosa. Penelitian diawali dengan memfermentasi jus kubis selama 6 hari pada kondisi anaerobic fakultatif. Hasil fermentasi diisolasi dengan media de man rogosa and sharpe (MRS) yang ditambahkan CaCO3 1% dan diinkubasi selama 24 jam. Reinokulasi dilakukan sebanyak 5 kali sampai ditemukan kultur murni BAL, kemudian diidentifikasi dengan menggunakan kit analytical profile index (API) 50 CHL. Hasil identifikasi kemudian diuji kemampuannya untuk mendegradasi selulosa pada media MRS yang ditambahkan 1% carboxymethyl cellulosa (CMC) dan 0,1% congored sebagai indikator dan diinkubasi selama 24 jam. Hasil penelitian menunjukkan bahwa strain bakteri yang teridentifikasi adalah Lactobacillus plantarum dan Lactobacillus brevis yang dapat mendegradasi selulosa. Kesimpulan dari penelitian ini adalah ditemukannya 2 jenis bakteri asam laktat dari jus kubis terfermentasi yaitu Lactobacillus plantarum dan Lactobacillus brevis yang mempunyai sifat selulolitik.Isolation and Identification of Lactic Acid Bacteria Cellulolitik Originated from Fermented Cabbage JuiceAbstractThe objective of this study was to isolate and identify the type of lactic acid bacteria (BAL) of cellulolytic originating from fermented cabbage juice. Research was conducted with the following steps: microbial isolation from fermented cabbage juice, cell morphology characteristics, biochemical characteristics, identification with an analytical profile index (API) 50 CHL kit, and cellulose degradability testing. The research was begun by fermenting cabbage juice for 6 days under facultative aerobic condition. The fermentation product was then isolated with de man rogosa and sharpe (MRS) medium which added CaCO3 1% and incubated for 24 hours. Reinoculation was performed 5 times until BAL was found. BAL pure cultures were used to identify lactic acid bacteria strains using an analytical profile index (API) 50 CHL kit. After pure culture was found, it was then tested for the ability to degrade cellulose on MRS media with 1% carboxymethyl cellulose (CMC) and 0.1% congored as indicator and incubated for 24 hours. The results showed that the identified bacterial strains were Lactobacillus plantarum and Lactobacillus brevis which could degrade cellulose. The conclusion of this research was the discovery of two types of lactic acid bacteria from fermented cabbage juice namely Lactobacillus plantarum and Lactobacillus brevis which had cellulolytic properties.

Isolasi dan Identifikasi Bakteri Asam Laktat Selulolitik yang Berasal dari Jus Kubis Terfermentasi Cahya Setya Utama; Zuprizal Zuprizal; Chusnul Hanim; Wihandoyo Wihandoyo
Jurnal Aplikasi Teknologi Pangan Vol 7, No 1 (2018): Februari 2018
Publisher : Indonesian Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jatp.2155

Penelitian bertujuan mengisolasi dan identifikasi jenis bakteri asam laktat (BAL) selulolitik yang berasal dari jus kubis terfermentasi. Tahapan penelitian meliputi isolasi mikrobia dari jus kubis terfermentasi, karakteristik morfologi sel, karakteristik biokimiawi, identifikasi dengan kit api 50 CHL dan pengujian kemampuan mendegradasi selulosa. Penelitian diawali dengan memfermentasi jus kubis selama 6 hari pada kondisi anaerobic fakultatif. Hasil fermentasi diisolasi dengan media de man rogosa and sharpe (MRS) yang ditambahkan CaCO3 1% dan diinkubasi selama 24 jam. Reinokulasi dilakukan sebanyak 5 kali sampai ditemukan kultur murni BAL, kemudian diidentifikasi dengan menggunakan kit analytical profile index (API) 50 CHL. Hasil identifikasi kemudian diuji kemampuannya untuk mendegradasi selulosa pada media MRS yang ditambahkan 1% carboxymethyl cellulosa (CMC) dan 0,1% congored sebagai indikator dan diinkubasi selama 24 jam. Hasil penelitian menunjukkan bahwa strain bakteri yang teridentifikasi adalah Lactobacillus plantarum dan Lactobacillus brevis yang dapat mendegradasi selulosa. Kesimpulan dari penelitian ini adalah ditemukannya 2 jenis bakteri asam laktat dari jus kubis terfermentasi yaitu Lactobacillus plantarum dan Lactobacillus brevis yang mempunyai sifat selulolitik.Isolation and Identification of Lactic Acid Bacteria Cellulolitik Originated from Fermented Cabbage JuiceAbstractThe objective of this study was to isolate and identify the type of lactic acid bacteria (BAL) of cellulolytic originating from fermented cabbage juice. Research was conducted with the following steps: microbial isolation from fermented cabbage juice, cell morphology characteristics, biochemical characteristics, identification with an analytical profile index (API) 50 CHL kit, and cellulose degradability testing. The research was begun by fermenting cabbage juice for 6 days under facultative aerobic condition. The fermentation product was then isolated with de man rogosa and sharpe (MRS) medium which added CaCO3 1% and incubated for 24 hours. Reinoculation was performed 5 times until BAL was found. BAL pure cultures were used to identify lactic acid bacteria strains using an analytical profile index (API) 50 CHL kit. After pure culture was found, it was then tested for the ability to degrade cellulose on MRS media with 1% carboxymethyl cellulose (CMC) and 0.1% congored as indicator and incubated for 24 hours. The results showed that the identified bacterial strains were Lactobacillus plantarum and Lactobacillus brevis which could degrade cellulose. The conclusion of this research was the discovery of two types of lactic acid bacteria from fermented cabbage juice namely Lactobacillus plantarum and Lactobacillus brevis which had cellulolytic properties.

Pengaruh Suplementasi Tepung Kulit Manggis (Garcinia mangostana L.) dalam Ransum terhadap Produksi Karkas dan Perlemakan Ayam Broiler Sinta Maharani; Siska Fitria; Supadmo Supadmo; Zuprizal Zuprizal
ZOO INDONESIA Vol 25, No 1 (2016): Juli 2016
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v25i1.3019

Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi tepung kulit manggis (Garcinia mangostana L.) sebagai aditif pakan terhadap produksi karkas dan perlemakan ayam broiler. Sebanyak 72 ekor ayam broiler jantan strain Lohmann ditempatkan secara acak pada 12 buah kandang kelompok dengan perlakuan penambahan tepung kulit manggis 0; 0,5; 1,0; 1,5%. Masing-masing perlakuan terdiri dari 3 ulangan. Ayam broiler dipelihara selama 35 hari. Data dianalisis statistik dengan analisis variansi Rancangan Acak Lengkap Pola Searah. Setiap data dengan perbedaan yang nyata antar perlakuan diuji lanjut menggunakan Duncan’s new Multiple Range Test. Parameter yang diamati adalah produksi karkas dan perlemakan ayam broiler. Hasil penelitian menunjukkan bahwa penambahan 1,5% tepung kulit manggis dalam ransum menaikkan (P<0,05) persentase lemak abdominal dan kadar lemak daging ayam broiler umur 35 hari. Penambahan tepung kulit manggis tidak mempengaruhi produksi karkas dan kadar lemak subkutan. Dari hasil penelitian dapat disimpulkan bahwa suplementasi sampai 1,5% tepung kulit manggis dalam ransum tidak mempengaruhi produksi karkas maupun kandungan lemak subkutan ayam broiler.

The Effect of Nanoencapsulated Phaleria macrocarpa Fruits Extract in Drinking Water on Jejunal Histomorphology of Broiler Chickens N. Ningsih; B. Ariyadi; N. D. Dono; Supadmo Supadmo; Zuprizal Zuprizal
Tropical Animal Science Journal Vol. 42 No. 2 (2019): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

The purpose of this study was to investigate the characteristics of nanoencapsulated Phaleria macrocarpa fruits extract (NEPM) in drinking water and its effect on microbial population and histomorphology in the jejunal wall of broiler chickens. A total number of 200 male broiler chicks were distributed into 5 treatments with 4 replicates (10 birds in each replicate). The experimental treatments were control diet (T0; negative control), diet with tetracycline (T1; positive control), diet with 2.5% of Phaleria macrocarpa fruits extract (T2), diet with 2.5% NEPM (T3), and diet with 5.0% NEPM (T4). The diet was yellow corn and soybean meal that contains 20.44% CP, 2917.47 kcal/kg ME, 0.84% Ca, and 0.51% Pav. Variables evaluated were characteristics of NEPM, growth performance, intestinal microbial population (lactic acid bacteria (LAB) and Salmonella sp.), and intestinal histomorphology (villus height, crypt depth, and villus height to crypt depth ratio (VH : CD)). Data were analyzed using ANOVA in a completely randomized design. Orthogonal contrast test were used to separate mean of data when p-value differ significantly. Results showed that the size of NEPM was 778 nm with spherical shape and positive charges with the zeta potentials of +26.5 mV. Supplementing 5% of NEPM did not affect broiler growth performance, Salmonella sp. or crypt depth, but increased (p<0.05) LAB, villi height, and VH:CD. It can be concluded that 5.0% of NEPM in the drinking water had positive effect on the jejunal histomorphology and increased population of LAB while Salmonella sp. was not detected on all treatments.

Performance, Microbial Populations, and Jejunal Morphology of Broilers Supplemented with Nano-Encapsulated Graviola Leaf Extract U. Maesaroh; N. D. Dono; Zuprizal
Tropical Animal Science Journal Vol. 45 No. 1 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.1.64

This research was conducted to investigate the effects of adding nano-encapsulated graviola (Annona muricata Linn.) leaf extract (NGLE) to drinking water on microbial populations, jejunal morphology, and growth performance of broilers. A total of 300 seven-day-old Lohmann male broilers were allocated into 6 treatments with 5 replications and 10 chicks in each replicate pen. All birds were given the same basal diet but given drinking water treated with: drinking water only as a negative control (T1), drinking water + 25 mg/L Tetracycline (T2), drinking water + 15 mL/L GLE (T3), drinking water + 30 mL/L GLE (T4), drinking water + 15 mL/L NGLE (T5), or drinking water + 30 mL/L NGLE (T6). Variables observed in the current study included: body weight gain (BWG), final body weight (FBW), feed conversion ratio (FCR), feed intake (FI), carcass percentage, jejunal lactic acid bacteria (LAB), jejunal coliform bacteria, villus height and width, crypt depth, and crypt depth ratio. All variable data were statistically analyzed using a completely randomized design with one-way arrangement. Results showed that the addition of NGLE in drinking water improved (p<0.05) jejunal morphology, jejunal LAB, and growth performance of broiler chickens. The height of jejunal villus and population of jejunal LAB increased (p<0.01) when NGLE up to a dose of 15 mL/L was added into the drinking water. Supplementing 15 mL/L NGLE reduced (p<0.01) feed conversion ratio and improved (p<0.01) final body weight and carcass production compared with the other treatments. It is concluded that supplementation of 15 mL/L NGLE might be useful as an alternative for antibiotics growth promoters in poultry.

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