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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian E-jurnal Agro-Industri Indonesia Reaktor Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrointek Jurnal Penelitian Pascapanen Pertanian Jurnal Penelitian dan Pengembangan Pertanian Buletin Teknologi Pasca Panen Journal of Engineering and Management in Industrial System Indonesian Journal of Business and Entrepreneurship (IJBE) Journal of Engineering and Technological Sciences agriTECH Jurnal Sumberdaya Hayati Science and Technology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology EDUFORTECH Journal of Tropical Crop Science Menara Perkebunan Journal of Applied Engineering and Technological Science (JAETS) Indonesian Journal of Electrical Engineering and Computer Science Jurnal Penelitian dan Pengembangan Pertanian International Journal of Mechanical Engineering Technologies and Applications (MECHTA) Jurnal Agroekoteknologi Terapan ABDI PUSTAKA: Jurnal Perpustakaan dan Kearsipan Proceedings of International Conference on Multidiciplinary Research Journal of Applied Agricultural Science and Technology Prosiding Seminar Nasional Universitas Serambi Mekkah PERTANIAN TROPIK Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Makara Journal of Technology Jurnal Natural Journal of Sciencce Technology and Visual Culture JURNAL REKAYASA KIMIA & LINGKUNGAN
Titi Candra Sunarti
Departemen Teknologi Industri Pertanian Fakultas Teknologi Pertanian Institut Pertanian Bogor Jl. Lingkar Akademik, Kampus IPB Darmaga, Bogor 16680; Telp. (0251) 8621974
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Journal : Jurnal Sumberdaya Hayati

 1 
Seleksi Bakteri Asam Laktat dan Pemanfaatannya Sebagai Starter Kering Menggunakan Matriks Tapioka Asam Ira Erdiandini; Titi Candra Sunarti; Anja Meryandini
Jurnal Sumberdaya Hayati Vol. 1 No. 1 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.1.26-33

Abstract

The development of industrial fermentation food could not separate with the availability of culture starter that suffice to support its production. Dried starter can be an option to use in fermentation industry because it can be stored for longer time without rejuvenation. However, in the process of production of dried starter needs the matrix to maintain cell viability, economically and availability of raw material. This research was conducted to use selected dried starter of indigenous lactic acid bacteria by using sour cassava starch matrix. Eleven local isolates lactic acid bacteria isolates from spontaneous fermentation of carbohydrates commodity were selected based on their acid production capabilities and antibiotics susceptibilities. Isolate of E 1222 showed the best result and was identified as Pediococcus pentosaceus. The isolate was encapsulated with sour cassava starch matrix for making dried starter by using freeze dryer and spray dryer. Freeze dried starter culture could maintained the cell viability higher than spray dried starter culture i.e 10.34 log CFU/g and 8.91 log CFU/g, respectively. Finally, freeze dried starter culture could maintain the percentage of cell viability until 89.38% during four-weeks storage at 4 oC.
Formula Media Pertumbuhan Bakteri Asam Laktat Pediococcus pentosaceus Menggunakan Substrat Whey Tahu SAFITRI NURLAELA; TITI CANDRA SUNARTI; ANJA MERYANDINI
Jurnal Sumberdaya Hayati Vol. 2 No. 2 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.2.31-38

Abstract

The lactic acid bacteria (LAB) and its lactic acid are commonly used to preserve the food and to extend the food’s shelf life. MRS media is a growth medium for LAB, but it is not feasible for industrial scale application. Cheaper substrate from agriculture products is therefore required, such as tofu whey, which is potential to be used as LAB medium. Tofu whey contains important components to support the LAB growth, but it needs C source (5% of glucose) and nitrogen source (1% ammonium sulphate or urea) supplementations. This study aimed to investigate the influence of N-source to Pediococcus pentosaceus growth and its capability in producing acid compounds. The result showed that addition of urea increased pH fermentation, contrarily to that ammonium sulphate supplementation. The highest bacterial growth rate (μmaks) was observed on media with urea (0.43 jam-1), while the highest acid production was occured on media with ammonium sulphate (9.13 mg/mL). Supplementation of ammonium sulphate and urea on tofu whey highly supported the growth of bacterial population for about 6.5 × 108 CFU/mL and 5.4 × 108 CFU/ mL, respectively, but still lower compared to MRS media (2.03 × 1010 CFU/mL). 
Potensi Limbah Cair Industri Tapioka sebagai Media Pertumbuhan Starter Bakteri Asam Laktat Pediococcus pentosaceus E.1222 WULAN, RAHAYU; MERYANDINI, ANJA; SUNARTI, TITI CANDRA
Jurnal Sumberdaya Hayati Vol. 3 No. 1 (2017)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.3.1.27-33

Abstract

Fermentation of Lactic Acid Bacteria (LAB) Pediococcus pentosaceus can improve the quality of food and its shelf life. Using commercial LAB specific media, de Man Rogosa and Sharpe (MRS) for growth on industrial scale application is not efficient. Tapioca wastewater (TW) still contains some of the nutrients needed for the growth of P. pentosaceus, but needs the enrichment of carbon sources (5% of glucose) and nitrogen sources (ammonium sulfate). This study aimed to use tapioca industrial wastewater with the addition of glucose and ammonium sulfate as an alternative growth media for lactic acid bacteria P. pentosaceus E.1222. The results showed that glucose and nitrogen had no significant effect on the number of bacterial colonies. The highest substrate efficiency was tapioca wastewater (86.81%), MRS broth (53.73%), and TW with 5% of glucose and 1% of ammonium sulfate (43.53%) respectively. Maximum growth rate (μmaks) was found in TW with 5% of glucose and 1% of ammonium sulfate (0,52 hours-1). Increasing the starter volume until 1000 mL in TW with 5% of glucose and 1% of ammonium sulfate showed a slight decrease in the log number of bacteria from 8,836 (50 mL), 8,401 (500 mL), to 8,063 (1000 mL). 
Seleksi Bakteri Asam Laktat dan Pemanfaatannya sebagai Starter pada Fermentasi Biji Sorgum: Selection of Lactic Acid Bacteria and its Application as Starter for Sorghum Grain Fermentation Yuliana, Meta; Meryandini, Anja; Sunarti, Titi Candra
Jurnal Sumberdaya Hayati Vol. 5 No. 1 (2019)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.5.1.35-42

Abstract

This research aims is to find starter for sorghum fermentation and to observe its influence in fermentation process and tannin level in sorghum flour. Four isolates of Lactic Acid Bacteria were tested as starter for Numbu and Kawali sorghum grain fermentation. The criteria was the ability to produce acid and cell viability. The fermentation used four treatments in duplicates. The selected isolate was inoculated into dehulled and non-dehulled sorghum grain. The fermentation time were 0, 24, and 48 h. Analysis were pH, total acidity, total sugar, viable cell, tannin level, structure of sorghum flour, the yield of flour passed 80 mesh, and flour moisture. The results showed that E1222 was selected as a starter for Numbu sorghum grain fermentation while E5 was selected for Kawali. The starter can decrease pH value, increasing total acidity, decreasing total sugar, however there was no significant difference in viable cell in fermentation process. It decreased tannin level approximately 90% in Numbu fermented flour and 77% in Kawali. There was corrosion in starch structure of fermented flour, while the yield of flour passing through 80 mesh was increased. The best treatment was fermentation of dehulled and inoculated sorghum grain (So-St) in both of sorghum varieties.
Pemanfaatan Bakteri Asam Laktat (BAL) Amilolitik untuk Fermentasi Tepung Terigu dan Gandum: Using of Amylolytic LAB for Fermentation of White Wheat and Whole Wheat Flour Suyono, Meisy Nawang; Sunarti, Titi Candra; Meryandini, Anja
Jurnal Sumberdaya Hayati Vol. 10 No. 2 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.2.61-66

Abstract

The national demand for carbohydrates in flour continues to increase significantly due to the emergence of contemporary food products that use flour as the primary ingredient. One of the products used in producing food is sourdough, which is fermented flour. Lactic acid bacteria (LAB) have a role in food fermentation by producing lactic acid. Amylolytic LAB can produce amylase enzyme, a biocatalyst in the starch hydrolysis process. One of the products made from the fermentation of wheat flour is sourdough. This study aims to determine the character of amylolytic LAB in wheat and wheat flour fermentation. The research methods include rejuvenation of LAB, selection of isolates, fermentation of starchy materials, and characterization of fermented liquid. The selection of bacteria was based on cell viability and pH, which showed that isolated Pediococcus pentasaceus E1222 could be used as a starter for starchy fermentation. The highest total acid and cell viability were produced in 24-hour wheat and wheat flour fermentation. Fermented wheat flour and flour had the most favorable aroma and texture, and fermented flour had the most favorable taste. Isolate Pediococcus pentasaceus E1222 is an isolate that can be used in making sourdough.
Produksi Asam Asetat dari Pulp Kopi Robusta Menggunakan Saccharomyces cerevisiae dan Acetobacter aceti : Acetic Acid Production from Robusta Coffee Pulp using Saccharomyces cerevisiae and Acetobacter aceti Zahrani, Siti Mutia; Sunarti, Titi Candra; Meryandini, Anja
Jurnal Sumberdaya Hayati Vol. 11 No. 1 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.1.16-22

Abstract

The type of coffee commonly grown by Indonesian farmers is robusta coffee (Coffea robusta). The processing of robusta coffee has so far focused on producing coffee beans with good flavor, leaving behind coffee pulp waste that needs to be utilized. This study aims to utilize coffee pulp waste to produce vinegar through a fermentation process using Saccharomyces cerevisiae and Acetobacter aceti. Vinegar production begins by rejuvenating A. aceti in YPG media and creating a growth curve. The fermentation of coffee pulp into vinegar involves two fermentation stages: alcoholic fermentation and acetic acid fermentation. Observation of the culture at the end of fermentation includes pH value, total acetic acid content, sugar content, and substrate utilization efficiency in product formation. The growth curve of A. aceti reaches the log phase at the 6th to 8th hour with a µmax of 0.45 hours-1. Vinegar from coffee pulp has the highest total acetic acid in 10 days of alcoholic fermentation and 7 days of acetic acid fermentation, which is 2.37%, with a pH value of 3.02, a Yp/s value of 0.995, and a ∆S/S value of 0.69. The amount of acetic acid obtained does not fulfill the requirements of SNI 01-4371-1996.
Co-Authors Adisalamun Adisalamun Adriana, Savira Astri Adwiyani, Pustika Agus Setiyono AHMAD JUNAEDI Aisyah, Nadira Akhmad Endang Zainal Hasan Anas Miftah Fauzi Andi Nurul Aulia Sari Andika Susantri Ani Suryani Ani Suryani Ani Suryani Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anuraga Jayanegara Aris Purwanto Asep Wawan Permana Asep Wawan Permana, Asep Wawan Asrianti Basri Asrianti Basri Ayu Rahayu SARASWATI Bambang Prasetya Basri, Asrianti Basri, Asrianti Besty Maranatha Birahy, Deford Cristy Bruce A. Welt, Bruce A. Cahyana, Purwa Tri Christina Winarti Christina Winarti Dede Heri Yuli Yanto Dedi Fardiaz Devi Ambarwaty Oktavia Dewi Diniaty Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi Setyaningsih Eka Putri EKA RURIANI EKA RURIANI Eka Ruriani Elisa Julianti Elly Rosyidah Endang Warsiki Endang Warsiki Erliza Noor Euis Hermiati Evi Savitri Iriani Evi Savitri Iriani, Evi Savitri Faqih Udin FARAH FAHMA Fauzan, RM Muhammad Nur Februadi Bastian Feri Kusnandar Ferry Mutia Fiora Helmi Frangky J. Paat Glisina Dwinoor Rembulan Hadi Karia Purwadaria, Hadi Karia Hari Eko Irianto Hartami Dewi Hartrisari Hardjomidjojo Hasrul Satria Hasrul Satria, Hasrul Ifah Munifah Ika Amalia Kartika Ilham Marvie Indah Yuliasih IRA ERDIANDINI, IRA Irmayanti Irmayanti Irmayanti Irmayanti, Irmayanti Irvan Anwar Iskandar Lubis Kendri Wahyuningsih Kendri Wahyuningsih, Kendri Khaswar Syamsu Kirana Sanggrami Sasmitaloka Kurniawan Yuniarto Kurniawan, Dede Yudo Laily, Noer Lala Nurmala Machfud Machfud Mala Nurilmala Manalu, Lamhot Maranatha, Besty Marimin , Matasik, Delfania Maya Melati Mohamad Syamsul Ma’arif Mulyorini Rahayuningsih MUTIA, FERY NAOMI, APRILIA Nastiti Siswi Indrasti Nida El Husna Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nisfatin Shofiana Nur Richana Nuri Andarwulan Odi Andanu Ono Suparno Paramuji, Muji Pasaribu, Fatimah J Petrus Adam Pramana, Yanuar Sigit Purwanto, Yanuar Jarwadi Purwoko Purwoko RAMADHAN, ZULFA AULIA Ratna Ekawati Ratna Ekawati Ridwansyah Ridwansyah Rijanti Rahaju Maulani Rijanti Rahaju Maulani Rijanti Rahaju Maulani Rini Purnawati Rismawati . Roh santoso Ronny Soputan Rosyidah, Elly Ruka Yulia SAFITRI NURLAELA Salfauqi Nurman, Salfauqi Sanbein, Primus Sapta Raharja Setyo Purwanto Singgih Wibowo Sitti Rahbiah Akram SOFIYANTO, M. EDY Stanislaus Aditya Agung Sugiyanta Sukard Sumarni Nompo Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suryanegara, Lisman Suyono, Meisy Nawang Taufik Hidayat Tjahja Muhandri Tryanisa Ridla Amalia Utomo, Prasetyo Hadi Vestika Iskawati Wahidul Hijah Wahyu Widosari Wawan Hermawan Widiatmaka Widiatmaka Widosari, Wahyu WULAN, RAHAYU Yandra Arkeman Yaya Rukayadi Yuana Susmiati Yulia, Ruka YULIANA, META YULIN LESTARI Yulistika, Efri Yunus Triyonggo, Yunus Yusmiati Yusmiati Zahrani, Siti Mutia