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Yuni Ahda
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Journal : Halal Research Journal

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Optimization of Authentication Methods for Processed Chicken Meat Products Based on ND5 Gene qPCR Achyar, Afifatul; Safitri, Fira; Atifah, Yusni; Farma, Siska Alicia; Putri, Dwi Hilda; Ahda, Yuni
Halal Research Vol 5 No 2 (2025): July
Publisher : Halal Center ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j22759970.v5i2.1823

Abstract

Consumers have the right to correct information about the products they consume. One example of a food product that is vulnerable to counterfeiting is chicken nuggets. To overcome this problem, qPCR can be an effective solution. Previous research has designed primers targeting the chicken ND5 gene. However, the PCR conditions for this primer pair have never been optimized. This research aims to optimize qPCR conditions and carry out chicken nugget authentication trials. Genomic DNA was isolated from five weight variations of chicken meat samples as a qPCR standard curve and three chicken nugget samples. Optimizing the annealing temperature with gradient PCR gave the best results at a temperature of 57.6OC. The results of the authentication test showed that the chicken meat content in TD nuggets was 54.7%, C nuggets 43.8%, and D nuggets 37.3%. This value is still in accordance with the Indonesian National Standard (SNI).
Co-Authors Abdul Rasak Abdul Razak Abdul Razak Achyar, Afifatul Afandi, Echa Azkia Afriandi, Muhammad Agusminarti, Agusminarti Ahmad Fauzan Ahmad Saleh Akbar, Imam Qodri Ambiyar, Ambiyar Ananda, Azwir Anggun, Septiasri Angreni, Siska Anisha S Anni Faridah Arfianti, Fina Aripin, Nurul Atifah, Yusni Atmazaki Atmazaki Ayesa, Putri Ayu, Duni Azalia, Salsa Novela Azeli, Saskia Putri Azwir Anhar Azzahra, Yolanda Belia Putri BR Tarigan, Siti Nadiah Zahra D. N. Putri, Dini Daniel Happy Putra Darmanella Dian Eka Wati Darussyamsu, Rahmawati Dezi Handayani Diana Lyrawati Diana Susanti Dualom, Siti Dwi Hilda Putri Efdillah, Syilvia Eriza, Deni Septia Evi Suryawati Fachry Abda El Rahman Fadhil Ramadhan, Bintang FAJRINA, SUCI Fatni Mufit Ferdyan, Rhavy Festiyed Festiyed Fidela, Wita Fitri Arsih Fitri, Anisa Fitri, Lel Fitria, Davina Fitria, Yanti Gunawan, Revi Gina Haryanto Haryanto Hastutin, Sri Yuni Hayu, Elvira Heffi Alberida Herisanti, Dini Ilahi, Threesia Anugrah Indra Hartanto Iqra, Kuntum Nurul IRDAWATI Irdes Hidayana Siregar Isnaini, Isnaini Jörg Gromoll Junil Adri K. Sari, Jelly Keiko Kasy Billah Keiko Kasy Billah Khairunnisa Lasmana, Oria Linda Advinda Linda Wati Lufri Lufri Marcloryne Agnesia Masyitoh Masyitoh Maulina, Adinda Rizky Meisya, Divia Yuda Miftahul Jannah Monica, Della Trya Muhammad Ikbal Mulia, Nanta Muslima, Risa Ukhti Mutia Dwi Herakasih Mutia, Ravena Najib Rahman. G Neng Sholihat Nining Nuraida Nukman Moeloek Nurbaiti, Desy Nurpatri, Yeni NURUL HIDAYAH P. Ningky, Yiyin Pratama, Sandi Fransisco Purnomo Soeharso Rahma Amelia, Anisha Chahya Rahmadhani Fitri Rahmawati Darussyamsu Ramadhan Sumarmin Ratih Komala Dewi Ratna Nila Sari Regina Rinti Mutiara Sari Rona Taula Sari, Rona Taula safitri, fira Sagurung, Mariance Dartiani Salsabil, Velina Samsudin, Syakirah Santosa, Tomi Apra Sari, Rinti Mutiara Sefina, Nadia Sepriyani, Eria Marina Septelia I. Wanandi Siagian, Gunaria Siregar, Titik Ermila Sri Suciati Ningsih Syafrilianto, Syafrilianto Syahrastani, Syahrastani Syamsurizal Syari, Elsy Melia T. Berlian Taufiqa, Zuhrah Valofi, Nagra Aulia Wahyuni, Nur Anisa Wahyuni, Sisri Winda Ayu Fietri Yerimades, Yerimades Yerimadesi Yerimadesi Yerimadesi Yuda Meisya, Divia Yusi Febriani Zalni, Tita Yunia Zega, Dwi Junita Zhafira