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All Journal Biospecies Kolaboratif Bioeducation Journal Penelitian Pendidikan Biosfer: Jurnal Tadris Biologi BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan QUANTUM: Jurnal Inovasi Pendidikan Sains BIOMA : Jurnal Biologi Makassar Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Basicedu Jurnal Ilmu Kedokteran dan Kesehatan Biota Atrium Pendidikan Biologi Serambi Biologi Pelita Eksakta Journal of Stem Cell Research and Tissue Engineering Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi Jurnal Ekologi, Masyarakat dan Sains FORUM PAEDAGOGIK Jurnal Biosense BiosciED: Journal of Biological Science and Education Halal Research Journal TROPICAL GENETICS Jurnal Vokasi Mekanika Al Jahiz: Journal of Biology Education Research Jurnal Basicedu Tropical Genetics Jurnal Serambi Biologi Jurnal Pendidikan IPA Indonesia
Yuni Ahda
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Tropical Genetics

 1 
Genotyping of Sumatera local variety of citrus using random amplified polymorphism DNA (RAPD) technique Sari, Rinti Mutiara; Achyar, Afifatul A; Ahda, Yuni; Hilda Putri, Dwi
Tropical Genetics Vol. 2 No. 2 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i2.29

Abstract

Indonesia has local varieties of citrus that are no less than imported citrus, especially in terms of fruit freshness. However, people are more interested in the color of citrus peel so people prefer imported citrus to local citrus, especially in Sumatera. Therefore, it is necessary to carry out efforts to conserve and improve the characteristics of these citrus to improve their quality through plant breeding. This study aims to optimize DNA isolation methods for citrus fruit samples with Chelex-TE and to determine the genetic profile of local Sumatera citrus and imported citrus using the genotyping RAPD. The samples used were several local citrus in Sumatera (Citrus Siam Mountain Omeh, Citrus Madu, Citrus Keprok Maga, Citrus Keprok Brastepu and Citrus Pasaman) and imported Citrus (Citrus Sunkist, Citrus Clemengold, Citrus Murkot and Citrus Wokam). DNA was isolated using the 10% Chelex-TE method which was optimized for several parameters such as grain size, fruit skin and leaves. RAPD was performed using 10 RAPD primers. The results showed that the optimum 10% Chelex Chelex-TE isolation method was a sample size of 1 grain. The amplification of local Sumatran citrus and imported citrus using 10 single primers produced polymorphic bands. The value of jaccard's similarity indicates that the five samples of Sumatera local variety of citrus and imported citrus have high genetic variation. Indonesia has local varieties of citrus that are no less than imported citrus, especially in terms of fruit freshness. However, people are more interested in the color of citrus peel so people prefer imported citrus to local citrus, especially in Sumatera. Therefore, it is necessary to carry out efforts to conserve and improve the characteristics of these citrus to improve their quality through plant breeding. This study aims to optimize DNA isolation methods for citrus fruit samples with Chelex-TE and to determine the genetic profile of local Sumatera citrus and imported citrus using the genotyping RAPD. The samples used were several local citrus in Sumatera (Citrus Siam Mountain Omeh, Citrus Madu, Citrus Keprok Maga, Citrus Keprok Brastepu and Citrus Pasaman) and imported Citrus (Citrus Sunkist, Citrus Clemengold, Citrus Murkot and Citrus Wokam). DNA was isolated using the 10% Chelex-TE method which was optimized for several parameters such as grain size, fruit skin and leaves. RAPD was performed using 10 RAPD primers. The results showed that the optimum 10% Chelex Chelex-TE isolation method was a sample size of 1 grain. The amplification of local Sumatran citrus and imported citrus using 10 single primers produced polymorphic bands. The value of jaccard's similarity indicates that the five samples of Sumatera local variety of citrus and imported citrus have high genetic variation.
Dermatoglyphic analysis of asthma sufferers in a population of students at the faculty of mathematics and science, Universitas Negeri Padang Salsabil, Velina; Achyar, Afifatul; Ahda, Yuni
Tropical Genetics Vol. 2 No. 2 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i2.30

Abstract

Asthma is a disease defined as chronic inflammation of the airways which is characterized by recurrent coughing and shortness of breath. Dermatoglyphs are the appearance of tendrils and tendril patterns on the fingers, palms, toes and soles. Dermatoglyph is used as an aid in the detection of a number of diseases that have a strong heritable basis and is also used to detect abnormalities. This study aims to analyze and compare the patterns of dermatoglyphics in asthma sufferers and non-asthmatics on the fingertips. This type of research is descriptive research with cross sectional sampling method. Samples were taken from the student population of the Faculty of Mathematics and Natural Sciences, Padang State University as many as 100 samples of fingerprint patterns. The results obtained showed that there was a significant difference in the number of arch fingerprint patterns of asthmatics compared to normal individuals. Seeing this significant difference in numbers indicates a possible link between fingerprint patterns and asthma phenotypes.
Dermatoglyphic analysis of left-handed individuals in the student population of the Faculty of Mathematics and Natural Sciences Universitas Negeri Padang Herisanti, Dini; Achyar, Afifatul; Ahda, Yuni
Tropical Genetics Vol. 3 No. 1 (2023):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i1.41

Abstract

Dermatoglyphics is a science that studies fingerprint patterns that can be inherited genetically. Fingerprint patterns can be divided into three patterns, arch, loop, and whorl. Observation of fingerprints is used to determine fingerprint patterns and the relationship between these patterns and the fields of health, education, and criminology. Left-handed is a term for people who predominantly use their left hand as their main hand to carry out daily activities. This research aims to determine the relationship between fingerprint patterns and individuals who tend to carry out activities with the left side of their body (left-handed). The type of research carried out was descriptive research with a cross-sectional sample collection method. The sample was taken from the 2019-2022 student population of the Faculty of Mathematics and Natural Sciences, Padang State University, totaling 100 individuals. The samples were taken in November and December. This research was carried out at the Genetics and Biotechnology Laboratory, Faculty of Mathematics and Natural Sciences, Padang State University. The results of this study show that the dominant fingerprint pattern found in the left-handed individual phenotype is 16 fingerprint patterns, while normal individuals have 5 arch fingerprint patterns. This shows that there is a link between the phenotype of left-handed individuals and dermatoglyphics.
Co-Authors Abdul Rasak Abdul Razak Abdul Razak Achyar, Afifatul Afandi, Echa Azkia Afriandi, Muhammad Agusminarti, Agusminarti Ahmad Fauzan Ahmad Saleh Akbar, Imam Qodri Ambiyar, Ambiyar Ananda, Azwir Anggun, Septiasri Angreni, Siska Anisha S Anni Faridah Arfianti, Fina Aripin, Nurul Atifah, Yusni Ayesa, Putri Ayu, Duni Azalia, Salsa Novela Azeli, Saskia Putri Azwir Anhar Azzahra, Yolanda Belia Putri BR Tarigan, Siti Nadiah Zahra D. N. Putri, Dini Daniel Happy Putra Darmanella Dian Eka Wati Darussyamsu, Rahmawati Dezi Handayani Diana Lyrawati Diana Susanti Dualom, Siti Dwi Hilda Putri Efdillah, Syilvia Eriza, Deni Septia Evi Suryawati Fachry Abda El Rahman Fadhil Ramadhan, Bintang FAJRINA, SUCI Fatni Mufit Ferdyan, Rhavy Festiyed Festiyed Fidela, Wita Fitri Arsih Fitri, Anisa Fitri, Lel Fitria, Davina Fitria, Yanti Gunawan, Revi Gina Haryanto Haryanto Hastutin, Sri Yuni Hayu, Elvira Heffi Alberida Herisanti, Dini Ilahi, Threesia Anugrah Indra Hartanto Iqra, Kuntum Nurul IRDAWATI Irdes Hidayana Siregar Jörg Gromoll Junil Adri K. Sari, Jelly Keiko Kasy Billah Keiko Kasy Billah Khairunnisa Lasmana, Oria Linda Advinda Linda Wati Lufri Lufri Marcloryne Agnesia Masyitoh Masyitoh Maulina, Adinda Rizky Meisya, Divia Yuda Miftahul Jannah Monica, Della Trya Muhammad Ikbal Mulia, Nanta Muslima, Risa Ukhti Mutia Dwi Herakasih Mutia, Ravena Najib Rahman. G Neng Sholihat Nining Nuraida Nukman Moeloek Nurbaiti, Desy Nurpatri, Yeni NURUL HIDAYAH P. Ningky, Yiyin Pratama, Sandi Fransisco Purnomo Soeharso Rahma Amelia, Anisha Chahya Rahmadhani Fitri Rahmawati Darussyamsu Ramadhan Sumarmin Ratih Komala Dewi Ratna Nila Sari Regina Rinti Mutiara Sari Rona Taula Sari, Rona Taula safitri, fira Sagurung, Mariance Dartiani Salsabil, Velina Samsudin, Syakirah Santosa, Tomi Apra Sari, Rinti Mutiara Sefina, Nadia Sepriyani, Eria Marina Septelia I. Wanandi Siagian, Gunaria Siregar, Titik Ermila Sri Suciati Ningsih Syafrilianto, Syafrilianto Syahrastani, Syahrastani Syamsurizal Syari, Elsy Melia T. Berlian Taufiqa, Zuhrah Valofi, Nagra Aulia Wahyuni, Nur Anisa Wahyuni, Sisri Winda Ayu Fietri Yerimades, Yerimades Yerimadesi Yerimadesi Yerimadesi Yuda Meisya, Divia Yusi Febriani Zalni, Tita Yunia Zega, Dwi Junita Zhafira