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Karakteristik Sifat Fisikokimia Pati Garut (Maranta arundinaceae) Didah Nur Faridah; Dedi Fardiaz; Nuri Andarwulan; Titi Candra Sunarti
agriTECH Vol 34, No 1 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.996 KB) | DOI: 10.22146/agritech.9517

Abstract

The objective of this study was to determine the physicochemical characteristics of arrowroot starch. This research was conducted in two steps as follows: arrowroot starch extraction and characterization. A wet starch extraction method yielded 15.69% of arrowroot starch. The arrowroot starch contained starch of 98.10%, amylose 24.64%, amylopectin 75.36%, reducing sugar 4.94%, resistant starch 2.12% and in vitro starch digestibility of 84.35%. Proximate composition studies showed that the moisture content in the arrowroot starch sample is 11.48%, ash 0.34%, fat 0,68% and protein 0,24%. Gel permeation chromatography (GPC) profile of arrowroot starch using Toyopearl HW-65S gel gave mainly two fractions. The distribution of degree of polimerization (DP) of amylopectin using fluorophore-assisted capillary electrophoresis (FACE) indicated four groups of DP, i.e. DP 6-8. 9-12, 13-24 and 25-30. Scanning electron microscopy (SEM) showed that they consist of oval granules. The analysis by RVA showed that arrowroot starch had an A-type starch gelatinization profile. X-ray diffraction studies showed that all the arrowroot starch exhibited A-type diffractionpattern.ABSTRAKPenelitian ini bertujuan untuk mempelajari sifat fisikokimia pati garut. Penelitian dilakukan dalam dua tahap yaitu tahap ekstraksi pati garut dan karakterisasi pati garut. Metode ekstraksi basah menghasilkan rendemen 15,69% pati garut. Pati garut mengandung kadar pati, amilosa, amilopektin, gula pereduksi, pati resisten dan daya cerna pati masing-masing sebesar 98,10%; 24,64%, 75,36%, 4,94%, 2,12% dan 84,35%. Analisis proksimat pati garut mengandung air 11,48%, abu 0,34%, lemak 0,68% dan protein 0,24%. Profil gel permeation chromatography (GPC) dengan menggunakan Toyopearl HW-65S diperoleh 2 fraksi. Distribusi panjang rantai amilopektin diukur dengan fluorophore-assisted capillary electrophoresis (FACE) menunjukkan empat rentang derajat polimerisasi ( DP), yaitu DP 6-8. 9-12, 13-24 and 25-30. Scanning electron microscopy (SEM) mempelihatkan bahwa granula pati garut berbentuk oval. Pengujian Rapid Visco Analysis (RVA) menunjukkan pati garut memiliki profil gelatinisasi pati tipe A begitu pula hasil X-ray diffraction pati garut mempunyai kristalin tipe A.Kata kunci: Pati garut, GPC, FACE, RVA, X-ray diffraction, SEM
Kinetika Fotodegradasi Klorofil, Tokoferol, dan Karotenoid dalam Minyak Sawit Merah Dewi Fortuna Ayu; Nuri Andarwulan; Purwiyatno Hariyadi; Eko Hari Purnomo
agriTECH Vol 36, No 2 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (758.026 KB) | DOI: 10.22146/agritech.12855

Abstract

The objective of this research was to assess the photodegradation kinetics of chlorophyll, tocopherol, and carotenoid in red palm oil (RPO) during storage under flourescent light intensities of 5000, 10000, and 15000 lux. Photodegradation was followed by measuring the changes of chlorophyll, tocopherol, and carotene contents in RPO filled in transparent bottles stored in incubator box (31.60±0.69 oC) with controlled light intensity. As reference, pure RPO was filled in dark and transparent bottles then was stored at roomy temperature (31.46±1.04 °C) and lighting (476.25-484.89lux). The result showed that photodegradation of chlorophyll followed first order kinetics with two distinct photodegradation periods. First, rapid photodegradation period during the first 6 hours of storage with k values of 3.81x10-2, 4.45x10-2, 5.64x10-2 day-1, followed by a slower photodegradation period at prolonged storage more than 6 hours, with k values of 1.41x10-2, 3.01x10-2, 4.59x10-2 day-1 under light intensities of 5000, 10000, and 15000 lux, respectively. Photodegradation of tocopherol and carotene also followed first order kinetics. Photodegradation of tocopherol had the highest rate constant (k value of 9.10x10-2, 12.02x10-2, 17.33x10-2 day-1), meanwhile carotene had the lowest rate constant (k value of 0.80x10-2, 1.40x10-2, 1.98x10-2 day-1) under light intensities of 5000, 10000, and 15000 lux, respectively. The light intensity coefficient (zi) as dependence indicator of k on the changes of light intensity were measured. The zi values ofchlorophyll, tocopherol, and carotene were 20000, 33333, and 25000 lux, respectively; indicated that the degradation rate of chlorophyll was the most sensitive to the changes of light intensity. ABSTRAKPenelitian ini bertujuan untuk mengkaji laju fotodegradasi klorofil, tokoferol, dan karoten dalam minyak sawit merah (MSM) selama penyimpanan dalam kondisi terpapar cahaya fluoresen pada intensitas 5000, 10000, dan 15000 lux. Fotodegradasi dikaji dengan mengukur perubahan kadar klorofil, tokoferol, dan karoten MSM pada botol transparan yang disimpan dalam kotak inkubator (31,60±0,69 oC) dengan intensitas cahaya dipertahankan konstan. Sebagai pembanding, MSM murni dalam botol gelap dan transparan disimpan pada suhu (31,46±1,04 °C) dan pencahayaan normal laboratorium (476,25-484,89 lux). Hasil penelitian menunjukkan bahwa klorofil mengalami fotodegradasi mengikuti model reaksi ordo pertama dalam dua periode; yaitu periode fotodegradasi cepat selama penyimpanan 6 jam pertama dengan nilai konstanta laju (k) 3,81x10-2, 4,45x10-2, 5,64x10-2 hari-1, diikuti periode fotodegradasi lambat pada penyimpanan yang lebih lama dengan nilai k 1,41x10-2, 3,01x10-2, 4,59x10-2 hari-1 masing-masing pada intensitas cahaya 5000, 10000, dan 15000 lux. Fotodegradasi tokoferol dan karoten juga mengikuti model reaksi ordo pertama. Fotodegradasi tokoferol berlangsung dengan laju paling tinggi (nilai k 9,10x10-2, 12,02x10-2, 17,33x10-2 hari-1), sedangkan fotodegradasi karoten berlangsung dengan laju paling rendah (nilai k 0,80x10-2, 1,40x10-2, 1, 98x10-2 hari-1) masing-masing pada perlakuan intensitas cahaya 5000, 10000, dan 15000 lux. Konstanta intensitas cahaya (zi) sebagai indikator ketergantungan nilai k terhadap perubahan intensitas cahaya untuk klorofil, tokoferol, karoten berturut-turut adalah 20000, 33333, dan 25000 lux. Hal ini mengindikasikan bahwa laju degradasi klorofil paling sensitif terhadap perubahan intensitas cahaya.
Pengurangan Kadar Digliserida dan Asam Lemak Bebas dalam Minyak Sawit Kasar Menggunakan Adsorben Khoerul Bariyah; Nuri Andarwulan; Purwiyatno Hariyadi
agriTECH Vol 37, No 1 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1404.825 KB) | DOI: 10.22146/agritech.17009

Abstract

Indonesia is the world’s largest crude palm oil (CPO) producer and consumer in 2014. Components that affect the quality of CPO are diglycerides (DAGs) and free fatty acids (FFA). DAGs in palm oil are known as the precursor of 3-MCPD esters, while higher content of FFA could influence the oil stability. The contact of CPO with adsorbent could affect the present of DAG and FFA in CPO. The purpose of this study was to determine the best type of adsorbent in reducing DAGs and FFA in CPO with emphasis on the characteristics of the adsorbent and adsorbate. This study was carried out by using three different types of CPO quality and six different types of adsorbent (carbon active, MgO, Magnesol R-60, and 3 types of bleaching earth). The contact process of CPO with different adsorbents were carried out at a temperature of 50-60 °C (without vacuum) for adsorbents selection and 90 °C (under vacuum) for 30 minutes at a dose of adsorbent 1 and 3 %. The contact process of different adsorbents with CPO have not been able to reduce both DAGs and FFA significantly at the non vacuum condition in three differents CPO sample. The combination of MgO and bleaching earth type 1 could reduce FFA up to 70 % reaching the content of 14 % at vacuum conditions, but did not reduce DAGs of CPO. Different CPO quality and adsorbent characteristics will affect the reduction process of FFA and DAGs. ABSTRAKIndonesia merupakan negara produsen sekaligus konsumen minyak sawit kasar (Crude Palm Oil/CPO) terbesar di dunia pada tahun 2014. Salah satu komponen yang mempengaruhi kualitas CPO adalah digliserida (DAG) dan asam lemak bebas (ALB). DAG dalam minyak sawit adalah prekursor pembentuk senyawa karsinogen 3-MCPD ester, sedangkan ALB yang tinggi dapat mempengaruhi stabilitas minyak. Proses kontak adsorben ke dalam CPO akan mempengaruhi keberadaan kedua komponen tersebut. Tujuan penelitian ini adalah untuk menentukan jenis adsorben yang paling baik dalam mengadsorp digliserida dan asam lemak bebas dalam CPO dengan menitikberatkan pada karakteristik adsorben dan adsorbat. Penelitian dilakukan terhadap 3 jenis CPO (nilai ALB: 4, 6, dan 14) dan 6 jenis adsorben (arang aktif, MgO, Magnesol R-60, dan 3 jenis bleaching earth). Proses kontak dilakukan pada suhu 50 – 60 °C (tanpa vakum) untuk seleksi adsorben dan 90 °C (dengan vakum) selama 30 menit dengan dosis adsorben 1 dan 3 %. Proses kontak adsorben pada CPO dengan kondisi adsorpsi tanpa vakum belum dapat menurunkan DAG dan ALB secara signifikan terhadap ketiga jenis CPO. Kombinasi antara adsorben bleaching earth tipe 1 dan MgO dapat menurunkan ALB hingga 70 % pada CPO dengan ALB 14 % pada kondisi vakum, tetapi tidak dapat menurunkan DAG. Karakterisitik CPO dan adsorben mempengaruhi proses reduksi ALB dan DAG.
Pemurnian Produk Mono-Diasilgliserol (MDAG) Hasil Gliserolisis Kimia dengan Metode Demulsifikasi Krim Satiti Kawuri Putri; Purwiyatno Hariyadi; Mursalin Mursalin; Nuri Andarwulan
agriTECH Vol 40, No 1 (2020)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.857 KB) | DOI: 10.22146/agritech.48779

Abstract

Cream demulsification is one of the purification method for separating the glycerol residue contained as emulsion with MDAG’s product. This method could be used to break emulsion system by adding electrolyte solution. This research aims to compare the performance of calcium chloride and calcium nitrate as electrolyte solution that is used in the process of cream demulsification. MDAG used was Fully Hydrogenated Palm Kernel Oil (FHPKO) from glycerolysis process. Based on student t-test, there was no significant difference (p>0,05) detected between the products of cream demulsification method using calcium nitrate and calcium chloride as electrolyte solution. It was discovered that calcium nitrate reduced residual glycerol from 11,69±0.88% to 0,57±0,07%; with characteristics of MDAG’s products were free fatty acid (ALB) of 8,51±0,94%, monoacylglycerol (MAG) of 31,53±3,27%, diacylglycerol (DAG) of 19,46±0,52%, triacylglycerol (TAG) of 39,93±4,66% and yield of 81,00±3,61%.respectively. In other hand, calcium chloride reduced residual glycerol from 11,69±0,88% to 0,64±0,06%; with characteristics of MDAG’s products were ALB of 8,91±0,71%, MAG of 9,79±1,97%, DAG of 19,16±0,53%, TAG of 41,49±3,24% and yield of 80,00±4,00%, respectively. Therefore, calcium nitrate can be to replace calcium chloride as electrolyte solution in cream demulsification method, especially in reducing the glycerol residue.
PROTEIN HISTON PADA SIPUT GONGGONG BINTAN Strombus sp. SEBAGAI KANDIDAT PANGAN FUNGSIONAL Lily Viruly; Nuri Andarwulan; Maggy T. Suhartono; Mala Nurilmala
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 11 No. 1 (2019): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1114.559 KB) | DOI: 10.29244/jitkt.v11i1.22299

Abstract

ABSTRAKGonggong termasuk sejenis siput laut, biota endemik yang banyak hidup di pantai Pulau Bintan dan sekitarnya di Provinsi Kepulauan Riau. Gonggong merupakan ikon kota Tanjungpinang, Provinsi Kepulauan Riau. Sampai saat ini, penelitian gonggong masih sangat sedikit padahal siput ini merupakan spesies yang sangat potensial. Tujuan penelitian ini adalah untuk mengkarakterisasi protein histon dari siput gonggong Strombus sp. asal Bintan sebagai kandidat pangan fungsional. Karakterisasi profil protein menggunakan SDS-PAGE. Kadar protein pada gonggong dianalisis dengan menggunakan metode Bradford. Gonggong rebus bercangkang tebal diekstraksi dengan metode maserasi menggunakan pelarut etanol PA 95% dan uji aktivitas antimikroba menggunakan metode sumur. Asam amino dianalisis menggunakan HPLC. Hasil karakterisasi profil protein pada daging gonggong menunjukkan bahwa gonggong bercangkang tipis dan gonggong bercangkang tebal memiliki pita profil protein yang sama pada berat molekul 11-37 kDa, sedangkan profil protein pada lendir gonggong bercangkang tebal dan tipis memiliki pita protein yang sama pada berat molekul 37 kDa. Jenis protein pada spesies gonggong Bintan diprediksi merupakan protein histon karena hasil amplifikasi menggunakan primer protein histon H2A dan H2B didapatkan gen target pada 75 bp dan uji antimikroba pada bakteri S. aureus dan E. coli memiliki nilai DDH sebesar 25,65±0,02 mm dan 14,45±0,13 mm, sehingga diduga bahwa gonggong Bintan berpotensi sebagai kandidat pangan fungsional khas Kepulauan Riau. ABSTRACTGonggong is one of the sea snails, endemic species living on coastal waters of Bintan Island and surrounding islands of the Riau Islands Province. Sea snail gonggong is an icon of Tanjungpinang-Riau Islands Province. Until now, research this snail is the least, whereas it is potential species. The purpose of this study was to characterize histone protein from Bintan gonggong snail Strombus sp. as functional food candidate. Protein profiling used SDS-PAGE. Protein contents were analyzed by Bradford method. Boiled thick shelled gonggong were extracted by maseration method using ethanol PA 95% and antimicrobial activity tes using well method. Amino acid analized with HPLC. The result of characterization on protein profiles in meat gonggong showed that the thin-shelled and thick-shelled gonggong had the same band as protein profiles by 11-37 kDa and protein profiles in mucus gonggong were found the same band as protein profiles of 37 kDa. The type of protein in spesies Bintan gonggong had been predicted a histone protein because DNA identification using primer protein histone H2A and H2B had gen target of 75 bp. Antimicrobial activity test on S. aureus and E. coli bacteria had value DDH of 25.65±0.02 mm and 14.45±0.13 mm. In fact, gonggong snail was potentially as antimicrobial peptide, so it will make local functional food candidate from Riau Islands Province.
KARAKTERISTIK KOMPOSISI KIMIA RUMPUT LAUT MERAH (RHODOPHYCEA) Eucheuma spinosum YANG DIBUDIDAYAKAN DARI PERAIRAN NUSA PENIDA, TAKALAR, DAN SUMENEP Andarini Diharmi; Dedi Fardiaz; Nuri Andarwulan; ndang Sri Heruwati
Berkala Perikanan Terubuk Vol 39, No 2 (2011): Juli 2011
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.77 KB) | DOI: 10.31258/terubuk.39.2.%p

Abstract

he objective of research was to get the chemical composition of red algal(E. spinosum) from different coastal of water that have the potensial as a sourceof carrageenan. The analysis was conducted to analyze the chemical content ofthe algal (E. spinosum) of three coastal water were moisture content, ash content,protein content, fat content, carbohydrate content and dietary fiber content. Theresults of analysis chemical composition of red algal from different waters hadshowed that content of moisture content 19.55-21.27% , ash content 18.55-18.95%, protein content 4.85-5.59% content, fat content of 0.06-0.1% andcarbohydrate content 53.44-55.52 % while the total dietary fiber content of redalgal (E. spinosum) was 12.78-15;92%. The chemical composition of red algal E.spinosum of three coastal water no real effect unless total dietary fiber contentwas significantly diffrence between the coastal water.
SENYAWA BIOAKTIF TORBANGUN (Plectranthus amboinicus (Lour.) SPRENG) FRAKSI KLOROFORM INDUKSI APOPTOSIS PADA KANKER PAYUDARA (SEL MCF-7) IN VITRO Wahid Yulianto; Nuri Andarwulan; Puspo Edi Giriwono; Joko Pamungkas
Majalah Obat Tradisional Vol 22, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.642 KB) | DOI: 10.22146/tradmedj.24312

Abstract

Torbangun (Plectranthus amboinicus (Lour.) Spreng) is a medicinal plant that has been traditionally used in tropical countries to cure various illnesses. The objective of this study was to identify the active compounds in the chloroform fraction which have effect on the apoptosis-related genes expression of breast cancer MCF-7 cells. Apoptosis was observed morphologically using Hoechst nuclear staining. Expression of the genes was analyzed using Real-Time PCR. Chemical compounds of the plant fractions were determined using LC-MS. Result of cell morphology observation clearly indicated apoptosis after the treatment of the plant fraction. Increased expression of anti-apoptotic gene Bcl-2 could not prevent the cells from apoptosis. Expressions of p53 and p21 genes were increased significantly. The expressions of caspase 9, caspase 7 and caspase 1 were increased at concentration-dependent manner. Most of the compounds in the chloroform fraction are identified as diterpenoids which may contribute to the apoptosis inducing activity of the fraction.
Karakteristik Fisik dan Fitokimia Buah Kecombrang (Etlingera elatior (Jack) R.M. Sm) Mirna Isyanti; Nuri Andarwulan; Didah Nur Faridah
Warta Industri Hasil Pertanian Vol 36, No 2 (2019)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32765/warta ihp.v36i2.5267

Abstract

ABSTRAK: Buah kecombrang termasuk famili Zingiberaceae, dimanfaatkan sebagai rempah, flavor makanan, dan herbal tradisional. Penelitian ini bertujuan mengetahui karakteristik fisik dan identifikasi bioaktif fitokimia 3 klon buah kecombrang (warna ungu, merah, dan merah muda) secara kualitatif dan kuantitatif. Ekstrak diperoleh melalui maserasi serbuk selama 26 jam dengan 0.1% HCl (1:10) dalam methanol. Penelitian menunjukkan buah kecombrang merah muda memiliki karakteristik fisik dominan (panjang, diameter, keliling, berat, jumlah pipilan buah) dan warna tercerah. Sedangkan buah kecombrang ungu mengandung total fenolik, total flavonoid, dan aktivitas antioksidan tertinggi yaitu total fenolik 1,51±0,03 mg GAE/g ekstrak berat kering (bk), total flavonoid 0.38±0.03 mg QE/g ekstrak bk (ungu), aktivitas antioksidan 599.07±92.58 mg AAE/100 g (DPPH) dan 59.14±5.72 mg AAE/100 g ekstrak bk (FRAP). Analisis PCA (Principal Component Analysis) menunjukkan korelasi negatif antara karakteristik fisik dengan fitokimia dan aktivitas antioksidan. Kata Kunci: antioksidan, buah kecombrang, karakteristik fisik, karakteristik fitokimia, PCA. ABSTRACT: Kecombrang is belongs to the family of Zingiberaceae, as spice, food flavoring, traditional herbal medicine. The research aimed to study physical and characterize bioactive phytochemicals of 3 klons kecombrang fruit (purple, red, and pink) by qualitative and quantitative analysis. The extract was obtained through maceration of powder for 26 hours with 0.1% HCl (1:10) in methanol.  The result showed that pink kecombrang had dominant physical characteristics (length, diameter, circumference, weight, number of pieces of fruit) and brightest colors. While purple kecombrang contained the highest total phenol, total flavonoids and antioxidant activity which are 1.51 ± 0.03 mg GAE/g dw extract 0.38 ± 0.03 mg QE/g dw extract, 599.07 ± 92.58 mg AAE/100 g dw (DPPH method) and 59.14 ± 5.72 mg AAE/100 g dw (FRAP method) respectively. PCA analysis showed a negative correlation between physical characteristics and phytochemical characteristics and antioxidant activity.  Keywords: Kecombrang fruits, physical characteristics, phytochemical characteristics, antioxidant, PCA.
Potensi Biji Kedawung (Parkia timoriana (DC.) Merr) sebagai Bahan Pangan Fungsional Anik Nur Hidayati; Nuri Andarwulan; Ervizal A. M. Zuhud
Warta Industri Hasil Pertanian Vol 37, No 2 (2020)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32765/wartaihp.v37i2.6113

Abstract

ABSTRAK: Kedawung (Parkia timoriana (DC.) Merr.) merupakan salah satu jenis tanaman obat yang sudah lama dimanfaatkan secara tradisional. Kedawung termasuk tanaman dengan potensi ekonomi tinggi namun tergolong langka karena hanya ditemukan di sebagian kecil wilayah Indonesia. Penelitian ini bertujuan untuk menganalisis profil proksimat, profil fitokimia, profil asam lemak dan profil trigliserida biji kedawung. Bahan utama yang digunakan dalam penelitian adalah daging biji kedawung. Adapun metode yang digunakan yaitu ektraksi biji kedawung, analisis proksimat, analisis fitokimia, analisis profil asam lemak, dan analisis profil trigliserida. Kandungan fitokimia yang terdapat pada biji kedawung yaitu saponin. Sementara komposisi proksimat biji kedawung meliputi kadar air (2,33 %), kadar protein (28,68 %), kadar abu (3,27 %), kadar karbohidrat (44,49%), dan kadar lemak (21,23%). Kandungan asam lemak pada biji kedawung didominasi oleh asam palmitat (34,74 ± 1,51), asam oleat (22,72 ± 0,37), dan asam linoleat (9,130 ± 0.104). Selanjutnya, komposisi trigliserida biji kedawung yaitu UUU (triunsaturated) (2,82%), SUU (saturated-unsaturated-unsaturated) (17,57%), SUS (saturated-unsaturated-saturated) (37,33%), SSS (trisaturated) (32,22%), dan unknown trigliserida (7,56%). Berdasarkan kandungan fitokimianya biji kedawung berpotensi dijadikan sebagai bahan pangan yang aman bagi manusia serta sebagai penghasil minyak/lemak pangan.Kata kunci: bahan pangan, kedawung, lemak, pangan potensial , Parkia timoriana (D.C.) Merr.ABSTRACT: Kedawung (Parkia timoriana (DC.) Merr.) is one of the medicinal plant that has long been traditionally used. Kedawung is classified as plants with high economic potential but rare because it is only found in few regions in Indonesia. This study aimed to analyze the proximate, phytochemicals, fatty acids, and triglycerides profile of kedawung. The main ingredient used in this study were kedawung seeds. The method used was extraction of kedawung seeds, proximate analysis, phytochemical analysis, fatty acid profile analysis, and triglyceride profile analysis. The content of phytochemicals found in kedawung seeds was saponin. The proximate composition of kedawung  included water content (2.33%), protein content (28.68%), ash content (3.27%), carbohydrate content (44.49%), and fat content (21.23%). The content of fatty acids were dominated by palmitic acid (34.74± 1,51), oleic acid (22.72±0.37), and linoleic acid (9.13± 0.10). Furthermore, the composition of triglycerides were UUU (triunsaturated) (2.82%), SUU (saturated-unsaturated-unsaturated) (17.57%), SUS (saturated-unsaturated-unsaturated) (37.33%), SSS (trisaturated) (32.22%), and unknown triglycerides (7.56%). Based on phytochemical compound, kedawung seeds could be used as food ingredients that is safe for humans and also as source of food oils/fat.Keywords:  food ingredients, fat, kedawung, potential foods, Parkia timoriana (DC.) Merr.
Optimasi Pembuatan Produk Turunan Minyak Nabati Monoasilgliserol Secara Esterifikasi Enzimatis Prima Luna; Nuri Andarwulan; Tri Haryati
Jurnal Penelitian Pascapanen Pertanian Vol 8, No 1 (2011): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v8n1.2011.24-31

Abstract

Monoasilgliserol (MAG) dari asam laurat merupakan salah satu produk turunan dari minyak nabati yang memiliki keistimewaan, berfungsi sehagai emulsifier, pengawet pangan, sanitizer, dan food supplement untuk meningkatkan imunitas tubuh. Penelitian ini bertujuan mencari kondisi optimum pembuatan monoasilgliserol dengan parameter komposisi MAG melaiui proses esterifikasi menggunakan enzim lipase imobil sebagai katalis. Metode esterifikasi enzirnatis kontinyu dilakukan pada reaktor packed bed sirkulasi. Rancangan percobaan optimasi pada penelitian ini menggunakan Central Composite Design dari Response Surface Methods (RSM). Suhu dan waktu reaksi merupakan faktor percobaan penelitian ini. Kondisi reaksi esterifikasi enzimatis kontinyu rnenggunakan rasio gliserol/asam lemak (5:1); rasio pelarut/substrat 8,8:1 dan residence time 23,57 menit. Hasil reaksi esterifikasi menggunakan RSM menunjukkan persamaan kuadrat optimasi MAG adalah Y= - 61,700 + 6,088 x1+3,259 x2 - 0,065 x12 + 0,017 x1x2 - 1,792 x22 dan menghasilkan MAG maksimum pada suhu dan waktu reaksi optimum 46,92°C (47±0,5)°C and 1,1 jam. Hasil validasi reaksi esterifikasi sebanyak lima kali ulangan menggunakan enzim lipase pada kondisi di atas menghasilkan rendemen 81,09±2,99% dengan komposisi MAG 83,15±3,51%. Koefisien keragaman untuk validasi rendemen dan komposisi MAG yaitu 3,69 and 4,25%. MAG memiliki sifat fisikokimia: bilangan asam 1,78±008%, bilangan peroksida 0,49±0,14 meq O2/kg MAG, kadar gliserol bebas 0,26%, dan memiliki kisaran titik leleh 53-53,5°C. Synthesis Optimization of Monoacylglyserol by Enzimatic Esterification.Monoacyglycerol (MAG) from lauric acid is a superior product derived from vegetable oil, which can be used as emulsifier, food preservative, sanitizer, and food supplement for increased human immunity. The aim of this research was to obtain the optimum conditions for monoacylglycerol synthesis based on MAG content using commercialized lipase. The Central Composite Design of Response Surface Methods (RSM) was used to arrange the experiments. Temperature and reaction time were the two variables investigated in the present study. Continuous enzymatic esterification was conducted in a circulated packed bed reactor with glycerol/oil molar ratio 5: I, solvent/substrate ratio 8.8:1 (wt/wt) and residence time 23.57 minute. The result showed that the quadratic equation for synthesis optimization of MAG was Y= - 61,700 + 6,088 x1+3,259 x2 - 0,065 x12 + 0,017 x1x2 - 1,792 x22 which produced maximum MAG at an optimum temperature of 46.92°C (47±0.5) °C and reaction time for 1,1 hour. Using the optimized conditions, lipase esterification, which was repeated five times, produced 81.09±2.99% yield with MAG content of 83.15±3.51%. Coefficients of variance for validation of the yield and MAG content were 3.69 and 4.25%, respectively. The MAG synthesized had the following physicochemical properties: acid value 1.78±008%, peroxide value 0.49±0.14 meq O2/kg MAG, free glycerol content 0.26%, and melting point 53-53.5°C
Co-Authors - Mursalin . Elfi . Mursalin A.A. Ketut Agung Cahyawan W Adawiyah, Dede Ade Nugraheni Herawati Affandi, Arief R Afifah Z. Agista Afni, Sabri Ella Agustin, Denny Agustina, Lenny Ai Mahmudatussa'adah Ai Mahmudatussa’adah Ajeng Kurniasari Putri Al Manar, Primadika Aminudin Aminudin Andarini Diharmi Andri J. Laksana Anik Nur Hidayati Anton Apriyantono Ardiansyah . Arief Rakhman Affandi Awaludin, Mohamad Ayu C. Wulan Ayu Cahyaning Wulan Azis Boing Sitanggang Baiq Pebrianti Hidayati C Hanny Wijaya Dahrul Syah Deddy Muchtadi Dede R. Adawiyah Dede R. Adawiyah Dede R. Adawiyah Dede Robiatul Adawiyah Dedi Fardiaz Dedin F R Denny Agustin Denny Agustin Denny Agustin Desty G. Pratiwi Desty Gitapratiwi Desty Gitapratiwi Desty Gitapratiwi Desty Gitapratiwi Desty Gitapratiwi Dewi Fortuna Ayu Dewi Monita Sari Dewi, Fitriya N. Dian Herawati Dias Indrasti Dina Mariana Dina Mariana Dirayati Hanifah Dodik Briawan Drajat Martianto Drajat Martianto Dwi Fitriani Dwi Fitriani Dwi Ishartani Dwi Karmila Syafriyanti Dwi Rahmawati Dwi Rahmawati Dwiyitno Dwiyitno Dwiyitno Dwiyitno Eddy KemenadyKemenady Efriwati Efriwati Eko Hari Purnomo Elvira Syamsir Endang Prangdimurti Endang Sri Heruwati Endang Sri Heruwati Erka Fitria Ervizal A. M. Zuhud Ervizal Amir Muhammad Zuhud Ervizal AMZU Erwin Riyadi Fadhilah Nur’Azizah farida ariyani Farida Ariyani Feri Kusnandar Fifi Retiaty Firdaus, Safira Fitrah Ernawati FITRY TAFZI Friska Citra Agustia Gema Noor Muhammad Giri Rohmad Barokah Gitapratiwi, Desty Hanifah Nuryani Lioe Hari Eko Irianto Hari Eko Irianto Hartuti, Wiwi Henni Rizki Septiana Hidayati, Rima Hilda Utami Anwar I Nyoman Adi Putra I Wayan Rai Widarta IB Ketut Widnyana Yoga Ineke Kusumawaty Inneke Kusumawaty Inneke Kusumawaty, Inneke Ismail Saleh Ismail Saleh Jan Schwarzbauer Jan Schwarzbauer Juanda Reputra Kartika Sari Kemenady, Eddy Khoerul Bariyah Koji Ishiguro Koji Ishiguro Krisjati, Paulina Gandhes Dian Krisna Margaretta Malau Laksana, Andri J. Laksmi Istikasari Laksono Trisnantoro Larissa Dsikowitzky Larissa Dsikowitzky Layyinan Hafizha Khalish Liber Liber Lilis Nuraida Lily Viruly Lisa Norisza Sjahwil Lula Nadia M. Aman Wirakartakusumah M. Aman Wirakartakusumah Madaniyah, Siti Maggy T. Suhartono MAGGY T. SUHARTONO Maggy T. Suhartono Mala Nurilmala Manar, Primadhika Al Maria Afrida Maria Fransisca Njoman Maryuliano, Ilham Maya Melati MINTARSIH Mirna Isyanti Muhammad Fadillah Muhammad Hasjim Bintoro Djoefrie Hasjim Bintoro Djoefrie Mursalin . Mursalin Mursalin MURSALIN MURSALIN Muslim, Nizar Saeful Nancy Dewi Yuliana ndang Sri Heruwati Nugroho Indrotristanto Nur Aini . Nur Wulandari Nur Wulandari Nur, Ria Choriatul Nurcahali, Fani Palupi, Nurheni Sri Pamungkas, Joko Policybrief, Submission Pribadi, Moch Fadlal Islamay Pribadi, Moch. Fadlal Islamay Prijono, Mariana prima luna Primadhika Al Manar Purwiyatno Hariyadi Purwiyatno Haryadi Puspo Edi Giriwono Putri, Fiadini Putri, Permata Adinda R. Iis Arifiantini Ratih Dewanti -Hariyadi Ria Choriatul Nur Ria Noviar Triana Ria Noviar Triana Ria Noviar Triana Ria Noviar Triana Rimbawan , Rimbawan Rimbawan rina ekawati Rina Ekawati Rini Kesenja Rini Kesenja Rosy Hutami Rusdi Sabri Ella Afni Salsabila, Unik Hanifah Sandra Arifin Aziz Santi Dwi Astuti Santi Dwi Astuti Santi Dwi Astuti sapanli, kastana Satiti Kawuri Putri Satrya Dharmawan Septiana, Henni Rizki Septiani, Bunga SHANNORA YULIASARI Shannora Yuliasari Siswahyudianto Siti Madanijah Siti Madaniyah Slamet Budijanto Slamet Wahyudi Sri Widowati Sri Yuliani SRI YULIANI Sukamto, Ika Sumiyarsi Sutrisno Koswara Syafriyanti, Dwi Karmila Takahiro Noda Takahiro Noda Tanti Lanovia1,2)* Tensiska, Tensiska Tiara Aninditha Tien R. Muchtadi Titi Candra Sunarti Tjahja Muhandri Tri Haryati Tri Haryati Triana, Ria Noviar Tunggul Waloya Victoria Valentina Wahid Yulianto Wardhani, Ajeng Widyaningrum Kusuma Wibowo, Laksono Wincy Wincy Wincy, Wincy Winiati P. Rahayu Winiati Pudji Rahayu Yoga Putranda Yuliani, Sri Yulianti Yulianti YULIASARI, Shannora Yundari, Yundari Zada Agna Talitha Zita L. Sarungallo Zuhud, Ervizal A. M. Zulaikhah Zulaikhah Zulaikhah Zulaikhah Zulaikhah Zulaikhah, Zulaikhah