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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Soils BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Pascapanen Pertanian Indonesian Journal of Biotechnology Jurnal Sumberdaya Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology BIOEDUSCIENCE Jurnal Ilmu Nutrisi dan Teknologi Pakan Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Livestock and Animal Research Makara Journal of Science Jurnal Natural Berita Biologi Jurnal Perikanan Darat dan Pesisir (JPDP) Biodiversitas Journal of Biological Diversity
Anja Meryandini
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, IPB Darmaga Campus, Bogor 16680; Center For Biotechnology, International Research Institute, IPB University, IPB Darmaga Campus, Bogor 16680
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Veterinary

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ISOLASI BAKTERI SELULOLITIK DAN KARAKTERISASI ENZIMNYA Meryandini, Anja; Widosari, Wahyu; Maranatha, Besty; Sunarti, Titi Candra; Rachmania, Nisa; Satria, Hasrul
Makara Journal of Science Vol. 13, No. 1
Publisher : UI Scholars Hub

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Abstract

Isolation of Cellulolytic Bacteria and Characterization of the Enzyme. Four of cellulolitic bacteria isolates had been characterized. The determination of cellulase activity was conducted at the highest production time, using crude enzymes with the modification of Miller methods (1959) on pure cellulose substrates such as CMC (Carboxymethyl cellulose), Avicel and Filter paper Whatman No. 1 as well as agriculture waste such as rice straw, corn cob and banana peel. Cellulase from C4-4, C5-1, C5-3 and C11-1 showed optimum activity at pH 5, 70°C, pH 3.5, 90°C, pH 5, 80°C and pH 8, 70°C, respectively. Avicel is a appropriate substrate for C4-4 cellulase whereas CMC for the other three. C11-1 cellulase has the highest cellulase enzyme activity on rice straw substrate whereas C4-4 cellulase on banana peel substrates. C5-1 and C5-3 cellulase have relatively low cellulase activities in degrading substrates of agriculture waste. However, isolates of C5-1 and C5-3 have high cellulase activities on banana peel substrates.
PEMURNIAN DAN KARAKTERISASI XILANASE Streptomyces sp. SKK1-8 Meryandini, Anja; Widhyastuti, Nunuk; Lestari, Yulin
Makara Journal of Science Vol. 11, No. 2
Publisher : UI Scholars Hub

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PEMURNIAN DAN KARAKTERISASI XILANASE Streptomyces sp. SKK1-8 Meryandini, Anja; Widhyastuti, Nunuk; Lestari, Yulin
Makara Journal of Science Vol. 12, No. 2
Publisher : UI Scholars Hub

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Daya Simpan Probiotik Bakteri Asam Laktat asal Larva Black Soldier Fly Terenkapsulasi: Shelf-life of Encapsulated Lactic Acid Bacteria Probiotics from Black Soldier Fassah, Dilla Mareistia; Hairani, Atikah; Meryandini, Anja; Astuti, Dewi Apri; Wiryawan, I Komang Gede
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 22 No. 1 (2024): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.22.1.23-28

Abstract

Lactic acid bacteria as probiotic candidates can be isolated from black soldier fly larvae. This study aimed to develop encapsulated lactic acid bacteria (LAB) probiotics from BSF larvae and to evaluate the effect of encapsulation on probiotic viability during 12 weeks of storage at room temperature and 4°C. The experimental design was a factorial completely randomized design with two treatments: storage temperature and storagetime. The variables observed are the number of cells and the level of cell resistance. The result showed that based on the growth curve of LAB isolates from BSF larvae, they could be harvested at 16 hours of age to be produced as probiotics. Encapsulated LAB probiotics from BSF larvae had a population of lactic acid bacteri (LAB) 107 CFU g-1 and were able to maintain the cell viability of 99.3%. Temperature and storage timesignificantly affect (p<0.05) the viability of encapsulated LAB. It can be conclude that encapsulation was able to optimally maintain the LAB viability at temperature of 4°C for 4 weeks of storage. Key words: black soldier fly larvae, encapsulation, probiotic, storability, viability
Identifikasi Aktinomiset Selulolitik dan Xilanolitik Indigenous Utarti, Esti; Suwanto, Antonius; Suhartono, Maggy T; Meryandini, Anja
BERKALA SAINSTEK Vol 8 No 1 (2020)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v8i1.15941

Abstract

Lignoselulosa merupakan penyusun utama dinding sel tumbuhan, sehingga keberadaannya berlimpah di alam. Aktinomiset indigenous yang memiliki aktivitas selulolitik dan xilanolitik ekstraseluler berpeluang sebagai agens biokonversi limbah berlignoselulosa menjadi produk bermanfaat. Penelitian ini bertujuan untuk mendapatkan aktinomiset potensial yang memiliki aktivitas selulolitik dan xilanolitik. Penelitian ini diawali dari isolasi dan pemurnian aktinomiset indigenous asal lahan perkebunan kelapa sawit dan Taman Nasional Bukit Duabelas Jambi.Tahapan selanjutnya adalah penapisan aktivitas selulolitik dan xilanolitik dari aktinomiset, uji pertumbuhan aktinomiset pada mikrokristalin selulosa, dan identifikasi aktinomiset potensial berdasarkan karakter morfologi, fisiologi dan biokimia. Hasil penelitian menunjukkan bahwa aktinomiset isolat S2 yang diisolasi dari lahan perkebunan kelapa sawit mempunyai aktivitas selulolitik dan xilanolitik lebih baik dari keempat isolat lain. Aktinomiset isolat S2 juga mampu tumbuh secara lebih baik pada mikrokristalin selulosa. Aktivitas selulolitik, xilanolitik dan kemampuan tumbuh pada mikrokristalin selulosa dari aktinomiset isolat S2 menunjukkan potensinya sebagai agens pendegradasi material belignoselulosa. Berdasarkan identifikasi morfologi, fisiologi dan biokimia, aktinomiset isolat S2 tergolong dalam genus Streptomyces.
Bioethanol Production from Non-Conventional Yeasts Wickerhamomyces anomalus (Pichia anomala) and Detection of ADH1 Gene Fathiah, Muhammad Fadhil; Hartono, Faisal Diniamal; Astuti, Rika Indri; Listiyowati, Sri; Meryandini, Anja
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.221-228

Abstract

Bioethanol is an organic compound resulted from the fermentation of sugar substrates by microorganisms which is used as alternative energy sources. During bioethanol fermentation yeast are exposed to various fermentation stresses, including temperature, osmotic, and oxidative stresess. Such conditions may decrease ethanol production. We previously isolated fermentation-stress tolerance yeast isolates from traditional Balinese beverages, identified as Wickerhamomyces anomalus BT2, BT5, and BT6. However no data available regarding the bioethanol production of those isolates. Our study indicates that these strains could utilize various sugar substrates (glucose, xylose, maltose, sucrose) in oxidative fermentative media. The highest value of substrate utilization efficiency following 48 hours fermentation was shown by BT6 on glucose (61.02%), BT 2 on xylose (55.44%) and maltose (60.90%). Measurement of ethanol production by Gas Chromatography showed that the strains were able to produce higher ethanol on the glucose substrate than other substrates. For instance, BT6 could produce the highest ethanol production (5.00 g/L) amongst strains tested by using glucose as substrate. Yet, the particular strains could only produce 0.30 g/L and 0.65 g/L by using xylose and maltose, respectively. For further genetic engineering purposes, we detected ADH1 gene from all three isolates, with high homology to the alcohol dehydrogenase from Saccharomyces cerevisiae, Geobacillus stearothermophilus and Pseudomonas aeruginosa. Further strain development can be carried out targeting the ADH1 gene, important for ethanol fermentation.
Molecular Analysis of Cry1Ab-Cry1Ac Gene Fusion in Transgenic Sugarcane Resistant to Shoot Borer Scircophaga excerptalis (Lepidoptera: Pyralidae) and Stem Borer Chilo sacchariphagus (Lepidoptera: Pyralidae) Armita, Dea; Meryandini, Anja; Suharsono; Koerniati, Sri
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1243-1251

Abstract

Sugarcane (Saccharum officinarum) is a plant with high economic value because it can produce sugar. Shoot borer Scircophaga excerptalis (Lepidoptera: Pyralidae) and stem borer Chilo sacchariphagus (Lepidoptera: Pyralidae) attacks are one of the issues that limit sugarcane productivity. Establishing transgenic sugarcane is one of the most efficient ways to prevent borer damage. Previously, Cry1Ab and Cry1Ac genes were successfully used to create transgenic sugarcane plants. This study aimed to detect the presence of transgenes and analyze the expression level of the Cry1Ab-Cry1Ac gene fusion in transgenic sugarcane using RT-PCR. The methods used in this study are transgene detection with PCR and gene expression analysis in normalized expression (2-ΔΔCq) with RT-PCR. The Cry1Ab-Cry1Ac gene has been integrated into all lines with varied expression levels. In 311 GV and 333 GV lines, the Cry1Ab-Cry1Ac gene was expressed in the leaf but not in the stem. Shoot and stem borer attack percentage values showed that all lines were lower than the control, with 222 EH as the lowest and 311 GV as the highest. Leaf and stem borer attack levels were compared to gene expression values of Cry1Ab-Cry1Ac. Results may indicate that the 222 EH line was resistant, but the 311 GV and 333 GV lines were not.
Potensi Limbah Cair Industri Tapioka sebagai Media Pertumbuhan Starter Bakteri Asam Laktat Pediococcus pentosaceus E.1222 WULAN, RAHAYU; MERYANDINI, ANJA; SUNARTI, TITI CANDRA
Jurnal Sumberdaya Hayati Vol. 3 No. 1 (2017)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.3.1.27-33

Abstract

Fermentation of Lactic Acid Bacteria (LAB) Pediococcus pentosaceus can improve the quality of food and its shelf life. Using commercial LAB specific media, de Man Rogosa and Sharpe (MRS) for growth on industrial scale application is not efficient. Tapioca wastewater (TW) still contains some of the nutrients needed for the growth of P. pentosaceus, but needs the enrichment of carbon sources (5% of glucose) and nitrogen sources (ammonium sulfate). This study aimed to use tapioca industrial wastewater with the addition of glucose and ammonium sulfate as an alternative growth media for lactic acid bacteria P. pentosaceus E.1222. The results showed that glucose and nitrogen had no significant effect on the number of bacterial colonies. The highest substrate efficiency was tapioca wastewater (86.81%), MRS broth (53.73%), and TW with 5% of glucose and 1% of ammonium sulfate (43.53%) respectively. Maximum growth rate (μmaks) was found in TW with 5% of glucose and 1% of ammonium sulfate (0,52 hours-1). Increasing the starter volume until 1000 mL in TW with 5% of glucose and 1% of ammonium sulfate showed a slight decrease in the log number of bacteria from 8,836 (50 mL), 8,401 (500 mL), to 8,063 (1000 mL). 
Seleksi Bakteri Asam Laktat dan Pemanfaatannya sebagai Starter pada Fermentasi Biji Sorgum: Selection of Lactic Acid Bacteria and its Application as Starter for Sorghum Grain Fermentation Yuliana, Meta; Meryandini, Anja; Sunarti, Titi Candra
Jurnal Sumberdaya Hayati Vol. 5 No. 1 (2019)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.5.1.35-42

Abstract

This research aims is to find starter for sorghum fermentation and to observe its influence in fermentation process and tannin level in sorghum flour. Four isolates of Lactic Acid Bacteria were tested as starter for Numbu and Kawali sorghum grain fermentation. The criteria was the ability to produce acid and cell viability. The fermentation used four treatments in duplicates. The selected isolate was inoculated into dehulled and non-dehulled sorghum grain. The fermentation time were 0, 24, and 48 h. Analysis were pH, total acidity, total sugar, viable cell, tannin level, structure of sorghum flour, the yield of flour passed 80 mesh, and flour moisture. The results showed that E1222 was selected as a starter for Numbu sorghum grain fermentation while E5 was selected for Kawali. The starter can decrease pH value, increasing total acidity, decreasing total sugar, however there was no significant difference in viable cell in fermentation process. It decreased tannin level approximately 90% in Numbu fermented flour and 77% in Kawali. There was corrosion in starch structure of fermented flour, while the yield of flour passing through 80 mesh was increased. The best treatment was fermentation of dehulled and inoculated sorghum grain (So-St) in both of sorghum varieties.
Aplikasi Lactobacillus plantarum NHC6 dalam Jus Nanas: Application Lactobacillus plantarum NHC6 as a Probiotic in Pineapple Juice Ariyanto, Yogy Satria; Meryandini, Anja; Sunarti, Titi
Jurnal Sumberdaya Hayati Vol. 7 No. 1 (2021)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.7.1.1-8

Abstract

Probiotics are living microorganisms if consumed in sufficient quantities can be beneficial for health. One of the potential bacteria as probiotics is Lactobacillus plantarum NHC6 is a lactic acid bacteria isolated from pineapple juice. This study aimed to test the ability of L. plantarum NHC6 as a probiotic in pineapple juice. Probiotics were stored until four weeks under 4°C and 10°C which weekly is monitored data on the amount of microbes, pH, percentage of lactic acid, and total dissolved solids in fermented pineapple juice. The result shows, the shelf time for pineapple juice under 4°C has a lower microbial reduction time rather than the juice storage under 10°C. Probiotics shelf time predicted for 4°C storage was 28.32 weeks and for 10°C storage was 8.67 weeks after production. The changes in physical properties (pH, percentage of lactic acid, and total soluble solids) in 10°C storage is much faster than that off in 4°C, this happens because there is still metabolic activity by bacteria at that temperature.
Co-Authors . Hamim . KURRATAA’YUN . YOPI . YOPI ., Yopi ., Yopi A, Gading Wilda A, Gading Wilda Abd. Rasyid Syamsuri Abdjad Asih Nawangsih Adawiah, Adilah Ade Andriani ADE ANDRIANI Ade Irma Ahmad Thontowi Aisyah, Nadira Alfred Michael ALINA AKHDIYA Alina Akhdiya AMOR TRESNA KARYAWATI Amor Tresna Karyawati, Amor Tresna Andika Susantri ANGGRAINI PUTRI UTAMI Anggreandari, Rizky Antonius Suwanto Anwar, Irvan Apon Zaenal Mustopa APRILIA NAOMI Ardana Kurniaji Ariandi Arina Amalia Putri Aris Tri Wahyudi Ariyanto, Yogy Satria Armita, Dea Asrianti Basri Atit Kanti Azizah Hikma Safitri Bambang Prasetya Bambang Prasetya Basri, Asrianti Basri, Asrianti Bedah Rupaedah Besty Maranatha CAHYA PRIHATNA Candra Sunarti , Titi Cocok Ana Maryani Berutu Cynthia Rizka Riani DAROJATUL ULYA Dede Heri Yuli Yanto Deden Saprudin Dedy Duryadi Solihin DEDY DURYADI SOLIHIN Deford Cristy Birahy DERI YURATMOKO DEWI APRI ASTUTI Dewi Asnita Dewi, Fitria Diana, Milsa Solva DIMAS ANDRIANTO DINI NURDIANI Dwi Ambarawati Dwi Andreas Santosa DWI SURYANTO DWI SURYANTO Dyah Iswantini Eka Putri EKA RURIANI Elly Rosyidah Engelhaupt, Martin Esti Utarti FAHRRUROZI FAHRRUROZI Fahrurrozi Fahrurrozi FAHRURROZI FAHRURROZI Fahrurrozi Fahrurrozi Fathiah, Muhammad Fadhil Fathin Hamida Faturrahman Faturrahman FATURRAHMAN FATURRAHMAN Ferry Mutia FERY MUTIA Fitri, Ainissya Fitria Dewi Glisina Dwinoor Rembulan GRISELDA HERMAN NATADIPUTRI Hairani, Atikah Hamim Hamim Hamtini - Hamtini Hanni Tsaaqifah Hari Eko Irianto Hartono, Faisal Diniamal Hasrul Satria Hasrul Satria Nur Hasrul Satria, Hasrul I Komang Gede Wiryawan Ici Dianita Ifah Munifah Ika Rahmatul Layly Iman Rusmana Inayah, Mazidah Noer IRA ERDIANDINI, IRA IRFAN ANWAR FAUZAN IS HELIANTI Isra Janatiningrum ISRA JANATININGRUM It Jamilah La Ode Wahidin LAKSMI AMBARSARI Lenni Fitri Lenni Fitri Lilis Nuraida Lily Nathalia Loli Natalia Lukman, Zulfiqar M. EDY SOFIYANTO Maggy T Suhartono Maggy T Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaya Suhartono Mahsunah, Anis Herliyati Malikah Azizah Manguntungi, Baso Maranatha, Besty Mareistia Fassah, Dilla Maria Sugiharti MARINI WIJAYANTI Marini Wijayanti MARTIN ENGELHAUPT Mernawati, Mernawati Muhammad Nur Kholis, Muhammad Nur Muhammad Subhan Hamka MUHAMMAD ZAIRIN JUNIOR MUNTI YUHANA NANIK RAHMANI Nanik Rahmani Nanik Rahmani Naswandi Nur Natalia, Loli Nathalia, Lily NI WAYAN SRI AGUSTINI Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nisfatin Shofiana NUNUK WIDHYASTUTI Nunuk Widhyastuti Nur Richana Nur, Hasrul Satria Nurfadhilla Rahmadhani Nurhasna, Aprilia Prima Agung Prihandono Puspitasari, Dian Japany Rahayu Wulan RAHAYU WULAN Rahayu Wulan RAMADHAN, ZULFA AULIA Rika Indri Astuti RIKA RAFFIUDIN Rismawati . Rizky Anggreandari RONI RIDWAN RONI RIDWAN Rosyidah, Elly RUSLI FIDRIYANTO SAFITRI NURLAELA SHANTI RATNAKOMALA Shanti Ratnakomala Sidiq, Muhammad Raffel SINTA AGUSTINA SINTA AGUSTINA SINTA AGUSTINA Sipriyadi Sipriyadi Sipriyadi Sitti Rahbiah Akram SRI ESTUNINGSIH Sri Estuningsih Sri Koerniati Sri Listiyowati Sri Suharti Stanislaus Aditya Agung SUHARSONO Sumarni Nompo Sunarti, Titi Suyono, Meisy Nawang Titi Candra Sunarti TRIO HENDARWIN Trio Hendarwin TRIO HENDARWIN Tryanisa Ridla Amalia Turnip, Enita Romasni UMI FATMAWATI Umi Fatmawati Vestika Iskawati Wahidul Hijah Wahyu Afrilasari Wahyu Widosari WENANG MAHARSIWI WIDANARNI WIDANARNI Widosari, Wahyu WILFADRI PUTRA JONESTI WULAN, RAHAYU Yantyati Widyastuti Yantyati Widyastuti Yaya Rukayadi YAYA RUKAYADI YAYA RUKAYADI Yopi - Yopi Yopi YOPI YOPI Yopi Yopi Yopi, YULIANA, META YULIN LESTARI Zahrani, Siti Mutia