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PROBIOTIK LOKAL MENINGKATKAN KANDUNGAN IgA USUS HALUS TIKUS YANG DIINFEKSI ENTEROPATHOGENIC E.Coli (EPEC): STUDI IMUNOHISTOKIMIA Wresdiyati, Tutik; Setiorini, Yeni; Laila, Sri Rahmatul; Arief, Irma Isnafia; Astawan, Made
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.921

Abstract

Penelitian ini bertujuan menganalisis pengaruh pemberian probiotik lokal Lactobacillus fermentum (L. fermentum) dan Lactobacillus plantarum (L. plantarum) terhadap profil kandungan IgA usus halus tikus yang diinfeksi enteropatogenik E. coli (EPEC) menggunakan teknik imunohistokimia. Sebanyak 90 ekor tikus jantan galur Sprague Dawley digunakan dan dibagi menjadi enam kelompok perlakuan, yaknikelompok kontrol negatif (A), kelompok perlakuan L. plantarum (B), kelompok perlakuan L. fermentum (C), kelompok perlakuan L. plantarumdan EPEC (D), kelompok perlakuan L. fermentum dan EPEC (E), dan kelompok perlakuan EPEC (F). Perlakuan dilaksanakan selama 21 hari. Deteksi IgA dilakukan dengan teknik imunohistokimia pada jaringan usus halus. Hasil penelitian menunjukkan bahwa perlakuan probiotik lokalL. fermentum selama 2-3 minggu, dan perlakuan L. plantarum selama 2 minggu mampu meningkatkan kandungan IgA di usus halus tikus. Pada tikus yang dipapar EPEC, L. fermentum lebih baik dalam meningkatkan kandungan IgA dibandingkan L. plantarum.
Extraction and Physicochemical Characterization of Pectin from Noni (Morinda citrifolia) Fruit Maulani, Tuti Rostianti; Lioe, Hanifah Nuryani; Arief, Irma Isnafia; Kusumaningrum, Harsi Dewantari
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 1, May 2024
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i1.37702

Abstract

Noni (Morinda citrifolia) is a popular plant in Asian countries and has great potential as pectin source for thickening agents in food products. In general, ethanol has been widely used to precipitate pectin, but the proportion can affect the quality of the product. Therefore, this study aimed to characterize the physicochemical properties of pectin from noni fruit. Extraction was performed using 0.1 N HCl at pH 1.5 and heated at 80°C for 15 min, followed by precipitation with different proportions of 96% ethanol including A (1:1), B (1:2), and C (1:3) v/v, while commercial orange pectin was used as a reference. The results showed that all pectin extracted belonged to the high methoxyl category. The FTIR spectra confirmed the presence of important functional groups including OH-hydroxyl, CH3-saturated aliphatic methyl, C=O carbonyl, and -O- cyclic ether. Furthermore, the crystalline and amorphous structures of noni pectin were confirmed by X-ray diffraction observation. The highest yield was achieved using the proportion of 1:3, followed by 1:2, and 1:1 on a wet basis. The MeO and AUA content of sample B (1:2) were relatively similar to those of the reference. Based on extraction results, noni possesses good characteristics and has the potential for commercial pectin production.
Characteristics and Antibacterial Activity of ZnO Nanoparticle Fortified Probiotic Yogurt Erlita Sari, Pipit; Abidin, Zaenal; Arief, Irma Isnafia; Budiman, Cahyo
Buletin Peternakan Vol 48, No 4 (2024): BULETIN PETERNAKAN VOL. 48 (4) NOVEMBER 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i4.94145

Abstract

Zinc deficiency is a major health issue in developing nations, especially in Southeast and South Asia. To address this, food fortification using nanotechnology, such as ZnO nanoparticles deemed safe by the US FDA, can enhance daily zinc intake essential for growth and immune health. This research emphasizes on yoghurt fortification with various types: ZnO nanoparticle fortification and the addition of probiotic bacteria Lactiplantibacillus plantarum subsp. plantarum IIA-1A5 (Accession Number: OR473281) and aims to analyze the nutritional value, characteristics, total lactic acid bacteria (LAB) and antibacterial activity of probiotic yogurt fortified with ZnO nanoparticles. There were six levels of treatment designed in this study and each treatment was tested three times. The statistical test used in analyzing the nutritional value, characteristics, total LAB and antibacterial activity in this study is the Tukey test which is applied as a multiple range test while in organoleptic testing using the Kruskal-Wallis non-parametric test. The results showed that the fortification of yogurt with ZnO nanoparticles had significant interaction (p<0.05) on yogurt viscosity, LAB content, and color attributes in hedonic quality test. The antibacterial activity towards Staphylococcus aureus and Escherichia coli produced by six treatments were moderate, with inhibition zones ranging between 5 to 10 mm. This study concluded that the fortified yoghurt treatment met the Indonesian National Standard (SNI), demonstrating the potential of this approach in addressing zinc deficiency in at-risk populations. This research underscores the promise of nanotechnology in food fortification strategies to combat micronutrient deficiencies globally
Peranan Probiotik Lactobacillus plantarum IIA-1A5 dalam Meningkatkan Ketahanan Tubuh, Performa, dan Kualitas Telur Ayam Komersial Agpretasia, Yodilla; Ulupi, Niken; Arief, Irma Isnafia
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.207

Abstract

Probiotics are microorganisms that are non-pathogenic and beneficial for health when used in certain doses through the mechanism of increasing the function of the small intestine in chickens. This study aims to analyze the effect of adding the probiotic Lactobacillus plantarum IIA-1A5 to feed to improve the body's immune system, production performance, and egg quality in laying hens aged 49‒52 weeks. The experimental design used was a Completely Randomized Design (CRD). The treatment for adding the probiotic Lactobacillus plantarum IIA-1A5 to feed consists of four treatment levels, namely P0 (without probiotics), P1 (feed + 8.1 x 106 cfu/g or 1 g/kg feed), P2 (feed + 2.43 x 107 cfu/g or 3 g/kg feed), and P3 (feed + 4.86 x 107 cfu/g or 6 g/kg feed). The results of the study showed that administering the probiotic Lactobacillus plantarum IIA-1A5 in feed could reduce Escherichia coli bacteria in the small intestine of laying hens. Giving the probiotic Lactobacillus plantarum also had a significant effect (P<0.05) on feed consumption and egg yolk color. The addition of the probiotic Lactobacillus plantarum IIA-1A5 in feed can increase animal resistance, performance, and quality of chicken eggs. The use of probiotics with a treatment level of 1 g kg-1 feed (8.1 x 106 cfu g-1) showed the best results. Keywords: heat stress, laying hens, probiotic
Co-Authors 'Izzati, Farah Diba . TRIVADILA Afton Atabany Agpretasia, Yodilla Ahmad Furqon Alfiansyah, Teuku Muhammad Alifiya, Qorina Amalina Nur Wahyuningtyas Ambar Mudigdo Amelia Friska Pertiwi Anas Qurniawan Andi Febrisiantosa Andi Nurul Mukhlisah Andri Kusmayadi Angelina, Putri Nur Apriadi Pasaribu Apriantini, Astari Aristo Kurniawan Sio Astari Apriantini Asti Yosela Oktaviana AYU LESTARI Bagus Priyo Purwanto Bahri Syamsuryadi Betty S. L. Jenie Cahyo Budiman Cece Sumantri Damiana Rita Ekastuti Darmawati, Maulita Putri Detiara, Azzahra Dondin Sajuthi Dono Indarto Dwi Febiyanti - Edi Erwan Ekajayanti Kining El Latifa Sri Suharto Epi Taufik Erlita Sari, Pipit Eti Rohaeti Fajri, Aulia Irhamni Faridah, Rajmi Febrina, Bunga Putri Firmansyah Firmansyah Fitra, Deni Fitri M Manihuruk Fitriananda, Putri Ghaling Achmad Guslian Abdul Basir Hajrawati Hajrawati Hanifah Nuryani Lioe Henny Nuraini Ida Ayu Putu Sri Widnyani Irmanida Batubara Isyana Khaerunnisa Isyana Khaerunnisa Iyep Komala J. Hermanianto Jakaria Jakaria Jaya Putra Jahidin Kamila, Elfa Aida Lilis Nuraida Luci Cyrilla Lucia Cyrilla Eko Nugrohowati Lucia Cyrilla Eko Nugrohowati Lucia Cyrilla ENSD Lucia cyrilla ENSD Lucya Cyrilla Eko Nugrohowati Supriyadi Dekriyatna Luki Abdullah MADE ASTAWAN Mahmud, Andi Tenri Bau Astuti Maulitia, Ziyan Tirta Mauludi, Muhammad Rifqi Mochammad Sriduresta Soenarno Monasdir Monasdir Monasdir, Monasdir Muh. Achyar Ardat Muhamad Arifin Muhamad Arifin, Muhamad Muhamad Syukur Muhammad Fikri Al Habib Muhammad Nur Hidayat Muhammad Yusuf Murtini, Devi Mutmainna, Andi Ni Wayan Triwulandhari Niken Ulupi Ninuk Purnaningsih Noraimah binti Sulaiman Noraimah Binti Sulaiman Noraimah Binti Sulaiman Nur Afni Nurul Hidayati Olfa Mega Panca Dewi Putra Jahtra Berutu R A Maheswari R. R. A. Maheswari Rahmawati, Wahyu Riski RIFKHAN RIFKHAN Rita Mutia Rudi Afnan Sadarman Sadarman Saiful Anwar Salsabila, Haditsah Salundik Salundik . Sari, Pipit Erlita Sena, Anantha Septi Dwi Utami Setyo Pertiwi Silaban, Yosua Kristianto Siti Aminah Sitta Fitri Rahmadhina Soenarno, M. Sriduresta Soenarno, Moch. Sriduresta sri murtini . Sri Rahmatul Laila Sri Sugiarti Suharyanto Suharyanto Suharyanto Suharyanto Sulassih, . Syah, Setiawan Putra Tjut Awaliyah Zuraiyah Tuti Rostianti Maulani Tuti Suryati Tutik Wresdiyati Vebriyanti, Ely Yandra Arkeman Yantyati Widyastuti Yeni Setiorini Yeni Setiorini Yong Soo Kim Yusuf Sumaryana Zaenal Abidin Zakiah Wulandari