Claim Missing Document
Check
Articles

Karakteristik Kefir Susu Sapi Dengan Penambahan Ekstrak Bunga Telang (Clitoria ternatea) Amelia Friska Pertiwi; Epi Taufik; Irma Isnafia Arief
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 1 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.1.34

Abstract

Kefir is made from a kefir seed starter consisting of L kefiri, genus Leuconostoc, Lactococcus, and Acetobacter that grow side by side. The addition of butterfly pea flowers extract to kefir can improve functional properties and sensory. The aim of this study was to investigate the physical characteristic, chemical characteristics, microorganisms, antioxidant activity, and organoleptic properties of cow’s milk kefir with the addition of butterfly pea flowers during storage at refrigerator temperature (1‒4°C). The research analysis used a completely randomized design with a 3x3 factorial pattern with three repetitions. The first factor was the addition of butterfly pea flowers (0%, 3%, and 6%), and the second factor was storage time (0, 3, and 6 days). The physical, chemical, antioxidant activity, and microorganism characteristics were tested using two-factor ANOVA and further tested by the Duncan test. Data on organoleptic properties were analyzed by the nonparametric Kruskal-Wallis and then verified using the Mann-Whitney test. The results showed that during storage the kefir increased the pH value and TAT value and decreased the viscosity and fat content. The inhibition of DPPH and the antioxidant capacity of the addition of butterfly pea flowers extract increased and decreased with the length of storage. Lactic acid bacteria (LAB) and the yeast did not change. Sensory hedonic test panelists prefer the color and aroma of kefir with the addition of telang flower extract. Sensory test of hedonic quality with the best characteristics, namely the addition of 6% butterfly pea flowers extract. Keywords: butterfly pea extract, kefir, storage organoleptic
Antibacterial Activity From Goat’s Milk Whey Hydrolized by Protease Enzyme of Bacillus Lincheniformis Irma Isnafia Arief; Sitta Fitri Rahmadhina; Cahyo Budiman
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol. 18 No. 1 (2023)
Publisher : Faculty of Animal Science Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitek.2023.018.01.3

Abstract

Food additives had been used to prevent and inhibit the growth of microorganisms and extend shelf life of food. Based on the safety of their used, it has an impact such as microorganism resistance to synthetic antimicrobials. This encourages the development of antimicrobials derived from natural ingredients, especially from milk. The bioactive components of milk protein have not always existed in their natural state, including precursors or peptides that will only be active if they have been hydrolyzed in vitro from their natural proteins. Hydrolysis of milk protein can be carried out using proteolytic enzymes from various sources, including protease extracted from Bacillus lincheniformis. This study used different concentrations of the protease extracted from Bacillus lincheniformis (0.5%, 1% and 1.5%) to produce hydrolyzate which has antibacterial activity in terms of protein content, SDS PAGE profile and the size of the inhibition zone (disc method). The results showed that the best activity of the whey hydrolyzed on Gram-negative Escherichia coli was 1% concentration of protease enzyme. In comparison, Gram positive Staphylococcus aureus can be inhibited with 0.5% concentration of protease enzyme.
PROBIOTIK LOKAL MENINGKATKAN KANDUNGAN IgA USUS HALUS TIKUS YANG DIINFEKSI ENTEROPATHOGENIC E.Coli (EPEC): STUDI IMUNOHISTOKIMIA Tutik Wresdiyati; Yeni Setiorini; Sri Rahmatul Laila; Irma Isnafia Arief; Made Astawan
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.986 KB) | DOI: 10.21157/j.ked.hewan.v7i2.921

Abstract

Penelitian  ini  bertujuan  menganalisis  pengaruh  pemberian  probiotik  lokal  Lactobacillus  fermentum  (L.  fermentum)  dan  Lactobacillus plantarum  (L.  plantarum)  terhadap profil kandungan IgA usus halus tikus yang diinfeksi   enteropatogenik  E. coli  (EPEC)  menggunakan teknik imunohistokimia.  Sebanyak  90  ekor  tikus  jantan  galur  Sprague  Dawley  digunakan  dan  dibagi  menjadi  enam  kelompok  perlakuan,  yaknikelompok kontrol negatif (A), kelompok perlakuan  L. plantarum  (B), kelompok perlakuan  L. fermentum  (C), kelompok perlakuan  L. plantarumdan EPEC (D), kelompok perlakuan  L.  fermentum  dan EPEC (E),  dan  kelompok perlakuan EPEC (F). Perlakuan dilaksanakan selama 21 hari. Deteksi IgA dilakukan dengan teknik imunohistokimia pada jaringan usus halus. Hasil penelitian menunjukkan bahwa perlakuan probiotik lokalL. fermentum selama 2-3 minggu, dan perlakuan L. plantarum selama 2 minggu mampu meningkatkan kandungan IgA di usus halus tikus. Pada tikus yang dipapar EPEC, L. fermentum lebih baik dalam meningkatkan kandungan IgA dibandingkan L. plantarum.
Karakteristik Fisikokimia, Mikrobiologi, dan Uji Pasar Yogurt Smoothies Rosela Stroberi pada Usia Remaja dan Dewasa di Bogor Putra Jahtra Berutu; Irma Isnafia Arief; Mochammad Sriduresta Soenarno; Lucya Cyrilla Eko Nugrohowati Supriyadi Dekriyatna
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 4 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.4.546

Abstract

Yogurt is a dairy product that is processed into a fermented drink using lactic acid bacteria. Smoothies are drinks made from fruit or vegetables containing milk, yogurt, or honey. Strawberry Rosella Yogurt Smoothie is yogurt that has been processed by reducing the water content and adding rosella flower extract and strawberry jam. Before being marketed, these products need to be tested for safety and market opportunities. This study aims to analyze the physicochemical characteristics, microbiology, and market tests of strawberry rosella yogurt smoothies in adolescents and adults in Bogor. The physicochemical characteristics tested included pH, water activity (aw), viscosity, and total titrated acid. The microbiological parameters tested were total lactic acid bacteria and Salmonella sp. The market parameters tested include analysis of consumer perceptions and preferences and their correlation with yogurt consumption patterns. The market test was carried out using 100 respondents consisting of 50 students from SMAN 1 Bogor and 50 students from IPB University. The results showed that the physical properties (pH, water activity, viscosity, and total lactic acid) fulfilled the SNI yogurt quality requirements. The chemical properties or nutritional content and microbiological properties of yoghurt smoothies have met the requirements for yoghurt quality standards based on SNI. The results showed that respondents had good perceptions and preferences on the attributes of smoothie yogurt. Consumer preferences regarding the appearance of the packaging in general, color, ingredient content, packaging information, and appearance of the contents of the yogurt are generally positively correlated with the price range of yogurt that consumers buy. Strawberry Rosella Yoghurt Smoothie is safe for consumption and can be marketed. Keywords: consumer perception, consumer preferences, microbiology, physicochemistry, yogurt smoothies
Synthesis, Characterization, Antibacterial Activity, and Potential Water Filter Application of Copper Oxide/Zeolite Composite Kamila, Elfa Aida; Abidin, Zaenal; Arief, Irma Isnafia; Trivadila, Trivadila
Makara Journal of Science Vol. 27, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Detection of E. coli bacteria in water samples can indicate the presence of other bacterial contamination in feces. Bacterial contamination can be treated with antibiotics, but excessive use of antibiotics leads to the emergence of multiresistant bacteria. Therefore, alternative antibacterial agents must be explored. Copper ion/zeolite composite has been widely studied as an antibacterial agent. However, the released copper ions accumulate in water and are toxic to humans; this can be prevented by converting copper ions into copper oxides (CuO and Cu2O). In this study, copper oxide/zeolite composite is synthesized and characterized using the heating method, followed by evaluation of its effect, antibacterial activity, and potential application as a water filter. The results showed that heat treatment and combination of zeolite with copper did not change the composition of zeolite, rather impurities were reduced from natural zeolites. Antibacterial activity test against E. coli showed that the copper oxide/zeolite composite possessed good antibacterial activity and exhibited better potential as a water filter compared with copper/zeolite. The copper ion released from the synthesized materials was under 2 mg/L, indicating that it is safe for use provided the minimum contact between the sample and water for less than 20 min or 50–60 min.
Extraction and Physicochemical Characterization of Pectin from Noni (Morinda citrifolia) Fruit Maulani, Tuti Rostianti; Lioe, Hanifah Nuryani; Arief, Irma Isnafia; Kusumaningrum, Harsi Dewantari
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 1, May 2024
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i1.37702

Abstract

Noni (Morinda citrifolia) is a popular plant in Asian countries and has great potential as pectin source for thickening agents in food products. In general, ethanol has been widely used to precipitate pectin, but the proportion can affect the quality of the product. Therefore, this study aimed to characterize the physicochemical properties of pectin from noni fruit. Extraction was performed using 0.1 N HCl at pH 1.5 and heated at 80°C for 15 min, followed by precipitation with different proportions of 96% ethanol including A (1:1), B (1:2), and C (1:3) v/v, while commercial orange pectin was used as a reference. The results showed that all pectin extracted belonged to the high methoxyl category. The FTIR spectra confirmed the presence of important functional groups including OH-hydroxyl, CH3-saturated aliphatic methyl, C=O carbonyl, and -O- cyclic ether. Furthermore, the crystalline and amorphous structures of noni pectin were confirmed by X-ray diffraction observation. The highest yield was achieved using the proportion of 1:3, followed by 1:2, and 1:1 on a wet basis. The MeO and AUA content of sample B (1:2) were relatively similar to those of the reference. Based on extraction results, noni possesses good characteristics and has the potential for commercial pectin production.
Antimicrobial activity of plantaricin IIA-1A5 on whey growth medium againts Pseudomonas aeroginosa: a scanning electron microscopy (SEM) study Mutmainna, Andi; Arief, Irma Isnafia; Lestari, Ayu; Hidayat, Muhammad Nur
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.24 KB) | DOI: 10.33772/jitro.v9i2.22444

Abstract

The study aimed to understand antimicrobial properties of plantaricin derived from L. plantarum IIA-1A5 and its inhibitory mechanism against Pseudomonas aeruginosa ATCC 27853 under SEM (scanning electron microscopy) observation. The inhibition of microbial growth was evaluated according to diameter of inhibitory zone. Scanning electron microscopy was then applied to observe the microstructure. The diameter of antimicrobial activity against Pseudomonas aeruginosa ATCC 27853 reached 6.52 mm in whey medium and 8.45 mm in whey+ medium (added with sucrose, tryptone and yeast extract). The experimental result clearly demonstrated the inhibition Pseudomonas aeruginosa ATCC 27853. Observation by SEM suggested that the antimicrobial activity of Pseudomonas aeruginosa ATCC 27853 related destruction of cellular morphology. The cell membrane was destroyed as indicated by the release of nucleic acid and reduction of membrane potential. In short, our experiment provided meaningful evidence on the future use of plantaricin IIA-1A5 as food preservative through disruption of cellular membrane causing leading to cell dying.Keywords:  Plantaricin, Lactobacillus plantarun, Pseudomonas aeruginosa, antimicrobial activity
The Characteristics of Dried Yoghurt and Market Test on Adolescent Consumers, Adults and Employees in Bogor City Monasdir Monasdir; Irma Isnafia Arief; Zakiah wulandari; Lucia Cyrilla Eko Nugrohowati
ANIMAL PRODUCTION Vol. 25 No. 3 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.3.212

Abstract

This study aimed to analyze the characteristics of dried yogurt, market potential, consumer perception, consumer purchasing behavior, and attributes that affect the purchase of dried yogurt made by teenagers, adults and employees in Bogor City. The results showed that based on testing the characteristics of dried yogurt with parameters of water content, pH, Aw, Viscosity, TAT and total lactic acid bacteria dried yogurt is safe for consumption. Based on the results of the conjoint analysis, it shows that to market dried yoghurt, producers must design dried yoghurt with probiotic content, then the product packaging must be in accordance with consumer tastes, in this case consumers prefer dried yoghurt with a packaging of 7 grams and priced at 10.000.Based on the results of the decriminant analysis, it shows that out of 100 respondents, as many as 90 people will buy dried yogurt if dried yogurt is marketed. Then 4 people will not buy dried yogurt if it is marketed and 6 people are hesitant about the choice. Based on the descriptive results of the study, it was shown that out of 100 respondents there were 47 women and 53 men with large families totaling 4 people, with an average income and expenditure a month of Rp. 500.000 - Rp. 1.500.000.
Evaluasi Good Dairy Farming Practice (GDFP) di Peternakan Sapi Perah Rakyat Kelompok Ternak Mandiri Sejahtera Cijeruk Bogor Iyep Komala; Irma Isnafia Arief; Afton Atabany; Lucia Cyrilla ENSD
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.19650

Abstract

ABSTRACT. Upaya yang dapat dilakukan untuk meningkatkan produksi dan kualitas susu dengan meningkatkan kapasitas SDM peternak dengan melakukan pendampingan untuk penerapan Good Dairy Farming Practices (GDFP). Penelitian ini bertujuan untuk mengevaluasi penerapan GDFP peternakan rakyat dataran tinggi pada Kelompok Ternak Mandiri Sejahtera Cijeruk Bogor. Metode yang digunakan yaitu metode survey dengan wawancara langsung kepada seluruh peternak sapi perah di Peternakan Mandiri Sejahtera sebanyak 22 orang, dengan menggunakan kuisioner yang mengacu kepada Ditjennak (1983) dan FAO (2011) yang dimodifikasi Andriyadi (2012). Berdasarkan karakteristik peternak nilai GDFP paling tinggi terdapat pada peternak dengan umur produktif 21-35 tahun (3.13), tingkat pendidikan D4/S1 (3.2) dan dengan pengalaman beternak 9-15 tahun (3.16). Berdasarkan faktor GDFP, nilai aspek GDFP tertinggi terdapat pada aspek manajemen pakan dan air minum (3.3) dan terendah pada aspek kandang dan peralatan (2.1). Penerapan GDFP pada Kelompok Ternak Mandiri Sejahtera tergolong cukup baik dengan nilai rataan 2.9. Peternak perlu melakukan perbaikan tata laksana pemeliharaan terutama pada aspek cara seleksi, pencatatan usaha, tata letak kandang dan penanganan limbah.(Evaluation of good dairy farming practice (GDFP) in smallholder dairy farms kelompok ternak mandiri sejahtera Cijeruk Bogor)ABSTRAK. Efforts that can be made to increase milk production and quality is by increasing the capacity of the breeders' human resources through providing assistance for the implementation of Good Dairy Farming Practices (GDFP). This study aim is to evaluate the implementation of GDFP on upland people's farms in the Kelompok Ternak Mandiri Sejahtera Cijeruk Bogor. The method used is a survey method with direct interviews to 22 dairy farmers in Mandiri Sejahtera Farms, using a questionnaire that refers to the Ditjennak (1983) and FAO (2011) which is modified Andriyadi (2012). Based on the characteristics of the farmer, the highest GDFP value is found in farmers with productive age of 21-35 years (3.3), education level of Diploma/Bachelor (3.2) and with 9-15 years of livestock experience (3.16). Based on the GDFP factor, the highest GDFP value was found in the feed and drinking water management aspect (3.3) and the lowest was in the cage and equipment (2.1). The implementing of GDFP in the Independent Prosperous Livestock Group is classified good with an average value of 3.0. Farmers need to improve maintenance management, especially in terms of selection, business records, cage layout and waste management.
Karakteristik Mikroorganisme, pH dan Unsur Hara Urin Sapi Perah di Daerah Bogor, Jawa Barat Ely Vebriyanti; Irma Isnafia Arief; Salundik Salundik; Panca Dewi
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.19844

Abstract

ABSTRACT. Penelitian ini bertujuan untuk menganalisis karakteristik mikroorganisme, pH, dan unsur hara pada urin sapi perah sebagai bahan dasar pembuatan biourin di daerah Bogor, Jawa Barat. Sampel penelitian ini adalah urin sapi perah hasil pengambilan urin pada waktu pagi dan sore. Umur sapi perah yang menjadi sampel penelitian adalah 3-4 tahun. Variabel dalam penelitian ini adalah mikroba, bakteri asam laktat (BAL), khamir, bakteri nitrifikasi, pH, NH4, kadar carbon (C), kadar phosfor (P), kadar nitrogen (N), kadar kalium (K), kadar besi (Fe) dam kadar tembaga (Cu). Hasil Penelitian menunjukkan bahwa pengambilan urin sapi pada pagi dan sore tidak pengaruh nyata terhadap total mikroba, total bakteri asam laktat, total kapang khamir, total bakteri nitrifikasi, pH, NH4, kadar phosfor (P), kadar nitrogen (N), kadar kalium (K), kadar besi (Fe) dam kadar tembaga (Cu). Kesimpulan penelitian ini adalah waktu pengambilan urin sapi pada pagi dan sore hari memberikan pengaruh nyata terhadap kadar C namun tidak berpengaruh nyata terhadap kadar K, kadar N, kadar Fe dan kadar Cu. Urin dengan waktu pengambilan pagi hari mengandung kadar C yang lebih tinggi dibandingkan dengan urin pengambilan sore. Hal ini menunjukkan bahwa urin sapi pagi dan sore atau gabungan urin pagi dan sore dapat digunakan sebagai bahan dasar pembuatan biourin sebagai pupuk organic cair.(Characteristics of microorganisme, macro and micro nutrients of dairy cattle urine at Bogor, West Java)ABSTRAK. This study aimed to analyze the characteristics of microorganisme pH and nutrients in the urine of dairy cows as the basic ingredients for making biourin in the Bogor area, West Java. The sample of this research is dairy cow urine with urine collection in the morning and evening. The age of the dairy cows in the research sample was 3-4 years. The parameters in this study were microbes, lactic acid bacteria (LAB), yeasts, nitrifying bacteria, pH, NH4, levels of carbon (C), levels of phosphorus (P), levels of nitrogen (N), levels of potassium (K), levels of iron ( Fe) and copper content (Cu). The results showed that the collection of cow urine in the morning and evening did not have a significant effect on total microbes, total lactic acid bacteria, total yeast molds, total nitrifying bacteria, pH, NH4, , phosphorus (P), levels of nitrogen (N), potassium content (K), iron content (Fe) and copper content (Cu). The conclusion of this study is that the time of taking cow urine in the in the morning and evening has a significant effect on K levels, N levels, Fe levels and Cu levels, urine taken in the morning contains higher levels of C than Urine taken in the afternoon. This shows that morning and evening cow urine or a combination of morning and evening urine can be used as the basic material for making biourine as liquid organic fertilizer.
Co-Authors . TRIVADILA Afton Atabany Ahmad Furqon Amalina Nur Wahyuningtyas Ambar Mudigdo Amelia Friska Pertiwi Anas Qurniawan Andi Febrisiantosa Andi Nurul Mukhlisah Andri Kusmayadi Angelina, Putri Nur Apriadi Pasaribu Apriantini, Astari Aristo Kurniawan Sio Astari Apriantini Asti Yosela Oktaviana AYU LESTARI Bagus Priyo Purwanto Bahri Syamsuryadi Betty S. L. Jenie Cahyo Budiman Cahyo Budiman Cahyo Budiman Cece Sumantri Damiana Rita Ekastuti Darmawati, Maulita Putri Detiara, Azzahra Dias Adiansyah Dondin Sajuthi Dono Indarto Dwi Febiyanti - Edi Erwan El Latifa Sri Suharto Epi Taufik Erlita Sari, Pipit Fajri, Aulia Irhamni Farah Diba 'Izzati Faridah, Rajmi Febrina, Bunga Putri Firmansyah Firmansyah Fitra, Deni Fitri M Manihuruk Ghaling Achmad Guslian Abdul Basir Hajrawati Hajrawati Hanifah Nuryani Lioe Henny Nuraini Ida Ayu Putu Sri Widnyani Irmanida Batubara Isyana Khaerunnisa Isyana Khaerunnisa Iyep Komala J. Hermanianto Jakaria Jakaria Jaya Putra Jahidin Jaya Putra Jahidin Kamila, Elfa Aida Lilis Nuraida Luci Cyrilla Lucia Cyrilla Eko Nugrohowati Lucia Cyrilla Eko Nugrohowati Lucia Cyrilla ENSD Lucia cyrilla ENSD Lucya Cyrilla Eko Nugrohowati Supriyadi Dekriyatna Luki Abdullah MADE ASTAWAN Mahmud, Andi Tenri Bau Astuti Maulitia, Ziyan Tirta Mochammad Sriduresta Soenarno Monasdir Monasdir Monasdir, Monasdir Muh. Achyar Ardat Muhamad Arifin Muhamad Arifin, Muhamad Muhamad Syukur Muhammad Fikri Al Habib Muhammad Nur Hidayat Muhammad Rifqi Mauludi Muhammad Yusuf Murtini, Devi Mutmainna, Andi Ni Wayan Triwulandhari Niken Ulupi Ninuk Purnaningsih Noraimah Binti Sulaiman Noraimah binti Sulaiman Noraimah Binti Sulaiman Nurul Hidayati Nurul Hidayati Olfa Mega Panca Dewi Putra Jahtra Berutu Putri Fitriananda Qorina Alifiya R A Maheswari R A Maheswari, R A R. R. A. Maheswari RIFKHAN RIFKHAN Rita Mutia Rudi Afnan Sadarman Sadarman Saiful Anwar Salsabila, Haditsah Salundik Salundik . Sari, Pipit Erlita Sena, Anantha Septi Dwi Utami Setyo Pertiwi siti aminah Sitta Fitri Rahmadhina Soenarno, M. Sriduresta Soenarno, Moch. Sriduresta sri murtini . Sri Rahmatul Laila Sri Sugiarti Suharyanto Suharyanto Suharyanto Suharyanto Sulassih, . Syah, Setiawan Putra Teuku Muhammad Alfiansyah Tjut Awaliyah Zuraiyah Tuti Rostianti Maulani Tuti Suryati Tutik Wresdiyati Vebriyanti, Ely Yandra Arkeman Yantyati Widyastuti Yeni Setiorini Yeni Setiorini Yodilla Agpretasia Yong Soo Kim Yosua Kristianto Silaban Yusuf Sumaryana Zaenal Abidin Zaenal Abidin Zaenal Abidin Zakiah Wulandari Zakiah Wulandari