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Therapeutic Potential of Synbiotic Roselle Extract Yogurt in Modulating Inflammatory Markers and Oral Microbiota in a Rat Model of 5-Fluorouracil-Induced Oral Mucositis Sena, Anantha; Mudigdo, Ambar; Arief, Irma-Isnafia; Indarto, Dono
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.58-70

Abstract

Mucositis is a common complication in cancer patients undergoing 5-fluorouracil (5-FU) chemotherapy. Roselle (Hibiscus sabdariffa) extract in synbiotic yogurt could be a beneficial alternative because it might change the inflammatory response and oral microbiota. The study aimed to investigate the effects of synbiotic roselle extract yogurt on inflammatory responses and oral microbiota in oral mucositis caused by 5-FU. An experimental study with twenty-four Sprague-Dawley rats divided into four groups (n=6): healthy control (NC), disease control (PC), synbiotic roselle extract yogurt therapy (P1), and standard therapy (P2) groups. This study evaluated the number of bacterial colonies, expression of COX-2 and caspase-1, and levels of IL-1β and VEGF (days 4 and 7). The P1 group had a significantly increased beneficial lactic acid bacteria (6.91±0.87) and a decrease in pathogenic bacteria, including Staphylococcus aureus (3.89±0.05), Escherichia coli (0), and Enterobacter aerogenes (1.78±0.29), compared to the PC group. Additionally, there was a statistically significant increase in VEGF levels in the tissue (0.07±0.03 ng/mL), a decrease in serum IL-1β levels (48.02±10.29 pg/mL), and an expression of caspase-1 and COX-2 compared to the PC group (p<0.05). Synbiotic roselle extract yogurt shows promise as a therapeutic strategy for managing mucositis by restoring microbial balance and mitigating inflammation.
PROBIOTIK LOKAL MENINGKATKAN KANDUNGAN IgA USUS HALUS TIKUS YANG DIINFEKSI ENTEROPATHOGENIC E.Coli (EPEC): STUDI IMUNOHISTOKIMIA Wresdiyati, Tutik; Setiorini, Yeni; Laila, Sri Rahmatul; Arief, Irma Isnafia; Astawan, Made
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.921

Abstract

Penelitian ini bertujuan menganalisis pengaruh pemberian probiotik lokal Lactobacillus fermentum (L. fermentum) dan Lactobacillus plantarum (L. plantarum) terhadap profil kandungan IgA usus halus tikus yang diinfeksi enteropatogenik E. coli (EPEC) menggunakan teknik imunohistokimia. Sebanyak 90 ekor tikus jantan galur Sprague Dawley digunakan dan dibagi menjadi enam kelompok perlakuan, yaknikelompok kontrol negatif (A), kelompok perlakuan L. plantarum (B), kelompok perlakuan L. fermentum (C), kelompok perlakuan L. plantarumdan EPEC (D), kelompok perlakuan L. fermentum dan EPEC (E), dan kelompok perlakuan EPEC (F). Perlakuan dilaksanakan selama 21 hari. Deteksi IgA dilakukan dengan teknik imunohistokimia pada jaringan usus halus. Hasil penelitian menunjukkan bahwa perlakuan probiotik lokalL. fermentum selama 2-3 minggu, dan perlakuan L. plantarum selama 2 minggu mampu meningkatkan kandungan IgA di usus halus tikus. Pada tikus yang dipapar EPEC, L. fermentum lebih baik dalam meningkatkan kandungan IgA dibandingkan L. plantarum.
Extraction and Physicochemical Characterization of Pectin from Noni (Morinda citrifolia) Fruit Maulani, Tuti Rostianti; Lioe, Hanifah Nuryani; Arief, Irma Isnafia; Kusumaningrum, Harsi Dewantari
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 1, May 2024
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i1.37702

Abstract

Noni (Morinda citrifolia) is a popular plant in Asian countries and has great potential as pectin source for thickening agents in food products. In general, ethanol has been widely used to precipitate pectin, but the proportion can affect the quality of the product. Therefore, this study aimed to characterize the physicochemical properties of pectin from noni fruit. Extraction was performed using 0.1 N HCl at pH 1.5 and heated at 80°C for 15 min, followed by precipitation with different proportions of 96% ethanol including A (1:1), B (1:2), and C (1:3) v/v, while commercial orange pectin was used as a reference. The results showed that all pectin extracted belonged to the high methoxyl category. The FTIR spectra confirmed the presence of important functional groups including OH-hydroxyl, CH3-saturated aliphatic methyl, C=O carbonyl, and -O- cyclic ether. Furthermore, the crystalline and amorphous structures of noni pectin were confirmed by X-ray diffraction observation. The highest yield was achieved using the proportion of 1:3, followed by 1:2, and 1:1 on a wet basis. The MeO and AUA content of sample B (1:2) were relatively similar to those of the reference. Based on extraction results, noni possesses good characteristics and has the potential for commercial pectin production.
Characteristics and Antibacterial Activity of ZnO Nanoparticle Fortified Probiotic Yogurt Erlita Sari, Pipit; Abidin, Zaenal; Arief, Irma Isnafia; Budiman, Cahyo
Buletin Peternakan Vol 48, No 4 (2024): BULETIN PETERNAKAN VOL. 48 (4) NOVEMBER 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i4.94145

Abstract

Zinc deficiency is a major health issue in developing nations, especially in Southeast and South Asia. To address this, food fortification using nanotechnology, such as ZnO nanoparticles deemed safe by the US FDA, can enhance daily zinc intake essential for growth and immune health. This research emphasizes on yoghurt fortification with various types: ZnO nanoparticle fortification and the addition of probiotic bacteria Lactiplantibacillus plantarum subsp. plantarum IIA-1A5 (Accession Number: OR473281) and aims to analyze the nutritional value, characteristics, total lactic acid bacteria (LAB) and antibacterial activity of probiotic yogurt fortified with ZnO nanoparticles. There were six levels of treatment designed in this study and each treatment was tested three times. The statistical test used in analyzing the nutritional value, characteristics, total LAB and antibacterial activity in this study is the Tukey test which is applied as a multiple range test while in organoleptic testing using the Kruskal-Wallis non-parametric test. The results showed that the fortification of yogurt with ZnO nanoparticles had significant interaction (p<0.05) on yogurt viscosity, LAB content, and color attributes in hedonic quality test. The antibacterial activity towards Staphylococcus aureus and Escherichia coli produced by six treatments were moderate, with inhibition zones ranging between 5 to 10 mm. This study concluded that the fortified yoghurt treatment met the Indonesian National Standard (SNI), demonstrating the potential of this approach in addressing zinc deficiency in at-risk populations. This research underscores the promise of nanotechnology in food fortification strategies to combat micronutrient deficiencies globally
The Effect of Milking Time and Post-Harvest Handling on the Quality of Fresh Milk in People Livestock Putri Fitriananda; Setyo Pertiwi; Irma Isnafia Arief
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 4 (2025): August 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v14i4.1243-1255

Abstract

Milk composition is essential in determining the quality of milk produced by farmers to meet the SNI 3141.1:2011. This study aimed to analyze and assess the physical, chemical, and biological quality of fresh milk from crossbred dairy cows at different milking times and harvest-postharvest handling. The experimental was designed using 2×2 Factorial Complete Randomized design with six repetitions. The independent variables in this study were milking time, including morning (P1), and afternoon (P2). Handling methods included conventional (V1), and modern (V2). Biplot analysis, and Multi-Criteria Decision-Making (MCDM) using the Analytic Hierarchy Process (AHP) method was performed. Milking time had a significant (p<0.05) on specific gravity, pH, TS, fat content, TSNF, protein, lactose, TPC, and S. aureus, which produced the best value at P1, except fat content. Harvest-postharvest handling had a significant (p<0.05) on pH, TPC, S. aureus, and coliforms, where V2 produced the best values compared to V1. Modern harvest-postharvest handling can reduce the TPC of fresh milk by 32.73%, S. aureus by 43.33%, and coliforms by 40.97% compared to that of conventional methods. Biplot analysis showed proximity between TPC, S. aureus and coliform. AHP optimization showed that morning milking time and modern handling methods are the most optimal combinations for producing best quality of fresh milk.   Keywords: Fresh milk, Harvest-postharvest handling, Milking time, Quality.
Diversifikasi Produk Susu Sapi Lokal melalui Pengolahan Keju Mozzarella dan Soft Candy di Ciawi, Bogor Irma Isnafia Arief; Nurul Hidayati; Zaenal Abidin; Tjut Awaliyah Zuraiyah
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 12 No. 2 (2026): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.12.2.228-240

Abstract

Ciawi, Bogor, represents a region with high potential in the agricultural sector, particularly dairy farming, where approximately 20% of local farmers are engaged in milk production. This condition provides opportunities for developing home industries that transform cow’s milk into value-added products. In response, Institut Pertanian Bogor (IPB), as a leading educational and research institution in Indonesia, has collaborated with PT. Bogor Sari Nutrisi (a local MSME/ Micro, Small and Medium Entreprises) and CV. Wahyu Farm Sejahtera (a dairy farm) to establish a program aimed at producing mozzarella cheese and soft candy from locally sourced cow’s milk. The project was implemented through a comprehensive situational analysis of local challenges and opportunities, dissemination of university-based innovations to industry, and collaborative partnerships with local enterprises. The initiative has resulted in improvements in production capacity and product quality, positioning mozzarella cheese and soft candy as potential regional specialty products. These findings demonstrate that innovation in milk processing can enhance the added value of local agricultural commodities, expand market opportunities, and contribute positively to regional economic development.
Karakteristik Fisikokimia, Kadar Gizi, Organoleptik dan Aktivitas Antioksidan dalam Es Krim Yoghurt Rosela Farah Diba &#039;Izzati; Irma Isnafia Arief; Cahyo Budiman; Zaenal Abidin
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 4 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.4.642

Abstract

This study develops roselle yogurt ice cream by combining the probiotic benefits of yogurt with bioactive compounds from roselle flowers. Yogurt is produced through milk fermentation by lactic acid bacteria, while roselle flower extract is rich in anthocyanins and flavonoids known for their antioxidant, antibacterial, and anti-inflammatory properties. The research methods included a comprehensive analysis of physicochemical properties, nutritional content, microbiology, antioxidant activity, and organoleptic characteristics. Using a randomized complete design, data analysis employed ANOVA and the Least Significant Difference tests (P < 0.05), with sensory evaluation conducted using the Kruskal-Wallis test. The results of exploring the synergy between roselle yogurt and ice cream in varying proportions (20:80, 30:70, 40:60) showed that the treatment with a 30:70 ratio of roselle yoghurt (P2) was optimal in physicochemical characteristics such as pH 5.47, total titratable acidity 0.74%, overrun 75.61%, and meltdown rate of 26.98 minutes. P2 also exhibited the highest total antioxidant activity at 137 µg/mL, with good sensory acceptance from consumers. This experiment provided an understanding of the potential of roselle yoghurt ice cream as a product that meets increasingly selective consumers' nutritional and health needs. It is hoped that these findings will stimulate the development of ice cream products that are not only commercially popular but also provide significant health benefits. Keywords: roselle yoghurt ice cream, antioxidant activity, consumer health
Peranan Probiotik Lactobacillus plantarum IIA-1A5 dalam Meningkatkan Ketahanan Tubuh, Performa, dan Kualitas Telur Ayam Komersial Yodilla Agpretasia; Niken Ulupi; Irma Isnafia Arief
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.207

Abstract

Probiotics are microorganisms that are non-pathogenic and beneficial for health when used in certain doses through the mechanism of increasing the function of the small intestine in chickens. This study aims to analyze the effect of adding the probiotic Lactobacillus plantarum IIA-1A5 to feed to improve the body's immune system, production performance, and egg quality in laying hens aged 49‒52 weeks. The experimental design used was a Completely Randomized Design (CRD). The treatment for adding the probiotic Lactobacillus plantarum IIA-1A5 to feed consists of four treatment levels, namely P0 (without probiotics), P1 (feed + 8.1 x 106 cfu/g or 1 g/kg feed), P2 (feed + 2.43 x 107 cfu/g or 3 g/kg feed), and P3 (feed + 4.86 x 107 cfu/g or 6 g/kg feed). The results of the study showed that administering the probiotic Lactobacillus plantarum IIA-1A5 in feed could reduce Escherichia coli bacteria in the small intestine of laying hens. Giving the probiotic Lactobacillus plantarum also had a significant effect (P<0.05) on feed consumption and egg yolk color. The addition of the probiotic Lactobacillus plantarum IIA-1A5 in feed can increase animal resistance, performance, and quality of chicken eggs. The use of probiotics with a treatment level of 1 g kg-1 feed (8.1 x 106 cfu g-1) showed the best results. Keywords: heat stress, laying hens, probiotic
Analisis Komponen Volatil Flavor Dendeng Batokok dengan Bahan Pengasap Serbuk Gergaji Medang Jahidin, Jaya Putra; Maheswari, R A; Arief, Irma Isnafia
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol 18 No 1 (2015): Mei 2015
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.763 KB) | DOI: 10.22437/jiiip.v18i1.2652

Abstract

Penelitian ini bertujuan untuk mempelajari komponen flavor yang berperan pada pengolahan dendeng batokok dari daging sapi dan kerbau sebagai bahan baku dengan bahan pengasap yang digunakan adalah serbuk gergaji kayu medang. Penelitian ini dirancang dengan menggunakan desain rancangan acak lengkap (RAL) dengan 3 (tiga) ulangan. Data non parametrik diolah menggunakan metode kruskal wallis, data parametrik peubah dianalisis dengan uji t. Pengolahan data komponen flavor menggunakan metode deskriptif dengan membandingkan dengan referensi yang ada. Hasil penelitan ini memperlihatkan bahwa komponen volatil fenol kemungkinan merupakan komponen yang memberikan pengaruh utama terhadap flavor dendeng batokok, sedangkan jumlah komponen volatil flavor yang terdapat pada masing-masing jenis daging yang digunakan juga berbeda.
Pelatihan Good Farming Practises, dan Good Manufacturing Practises untuk Meningkatkan Daya Saing UKM Abinisa Serang Banten Zakiah Wulandari; Niken Ulupi; Irma Isnafia Arief; Siti Aminah; Teuku Muhammad Alfiansyah; Muhammad Rifqi Mauludi; Qorina Alifiya; Yosua Kristianto Silaban
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. 1 (2025): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.11.1.20-28

Abstract

UKM Abinisa is a leading producer in the duck egg processing industry in Serang, Banten. To maintain competitiveness, SME need to focus on product innovation and implement Good Manufacturing Practices (GMP) and Good Farming Practices (GFP) to ensure product safety. This community service program aimed to provide training related to GMP and GFP. Socialization and training activities were held on the same date in Sujung Village, Tirtayasa Subdistrict, Serang Regency, Banten. The steps of the activity included: a) pre-activity preparation, b) internal coordination, c) implementation, and d) evaluation. The activity began with a visit to a duck farm owned by one of UKM Abinisa's farmer partners. The first training session covered techniques for proper and effective feeding, disease prevention, and maintaining healthy living conditions for ducks. Practical sessions included duck herding or releasing them onto post-harvest rice fields, a method well-suited to the extensive rice fields around Sujung Village. This method is known as the rice-duck integration system. The evaluation results showed a high level of participant satisfaction, with the highest-rated material being Material 1: Duck Farming, scoring 69.57%. Material 3 scored similarly at 65.22%. Material 2: Introduction to GMP received the lowest score, likely because of the complexity and detailed nature of the regulations, which were unfamiliar to all participants.
Co-Authors . TRIVADILA Afton Atabany Ahmad Furqon Amalina Nur Wahyuningtyas Ambar Mudigdo Amelia Friska Pertiwi Anas Qurniawan Andi Febrisiantosa Andi Nurul Mukhlisah Andri Kusmayadi Angelina, Putri Nur Apriadi Pasaribu Apriantini, Astari Aristo Kurniawan Sio Astari Apriantini Asti Yosela Oktaviana AYU LESTARI Bagus Priyo Purwanto Bahri Syamsuryadi Betty S. L. Jenie Cahyo Budiman Cahyo Budiman Cahyo Budiman Cece Sumantri Damiana Rita Ekastuti Darmawati, Maulita Putri Detiara, Azzahra Dias Adiansyah Dondin Sajuthi Dono Indarto Dwi Febiyanti - Edi Erwan El Latifa Sri Suharto Epi Taufik Erlita Sari, Pipit Fajri, Aulia Irhamni Farah Diba &#039;Izzati Faridah, Rajmi Febrina, Bunga Putri Firmansyah Firmansyah Fitra, Deni Fitri M Manihuruk Ghaling Achmad Guslian Abdul Basir Hajrawati Hajrawati Hanifah Nuryani Lioe Henny Nuraini Ida Ayu Putu Sri Widnyani Irmanida Batubara Isyana Khaerunnisa Isyana Khaerunnisa Iyep Komala J. Hermanianto Jakaria Jakaria Jaya Putra Jahidin Jaya Putra Jahidin Kamila, Elfa Aida Lilis Nuraida Luci Cyrilla Lucia Cyrilla Eko Nugrohowati Lucia Cyrilla Eko Nugrohowati Lucia Cyrilla ENSD Lucia cyrilla ENSD Lucya Cyrilla Eko Nugrohowati Supriyadi Dekriyatna Luki Abdullah MADE ASTAWAN Mahmud, Andi Tenri Bau Astuti Maulitia, Ziyan Tirta Mochammad Sriduresta Soenarno Monasdir Monasdir Monasdir, Monasdir Muh. Achyar Ardat Muhamad Arifin Muhamad Arifin, Muhamad Muhamad Syukur Muhammad Fikri Al Habib Muhammad Nur Hidayat Muhammad Rifqi Mauludi Muhammad Yusuf Murtini, Devi Mutmainna, Andi Ni Wayan Triwulandhari Niken Ulupi Ninuk Purnaningsih Noraimah Binti Sulaiman Noraimah binti Sulaiman Noraimah Binti Sulaiman Nurul Hidayati Nurul Hidayati Olfa Mega Panca Dewi Putra Jahtra Berutu Putri Fitriananda Qorina Alifiya R A Maheswari R A Maheswari, R A R. R. A. Maheswari RIFKHAN RIFKHAN Rita Mutia Rudi Afnan Sadarman Sadarman Saiful Anwar Salsabila, Haditsah Salundik Salundik . Sari, Pipit Erlita Sena, Anantha Septi Dwi Utami Setyo Pertiwi siti aminah Sitta Fitri Rahmadhina Soenarno, M. Sriduresta Soenarno, Moch. Sriduresta sri murtini . Sri Rahmatul Laila Sri Sugiarti Suharyanto Suharyanto Suharyanto Suharyanto Sulassih, . Syah, Setiawan Putra Teuku Muhammad Alfiansyah Tjut Awaliyah Zuraiyah Tuti Rostianti Maulani Tuti Suryati Tutik Wresdiyati Vebriyanti, Ely Yandra Arkeman Yantyati Widyastuti Yeni Setiorini Yeni Setiorini Yodilla Agpretasia Yong Soo Kim Yosua Kristianto Silaban Yusuf Sumaryana Zaenal Abidin Zaenal Abidin Zaenal Abidin Zakiah Wulandari Zakiah Wulandari