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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Buletin Peternakan VALENSI Jurnal Veteriner Jurnal Ilmu Ternak Majalah Kedokteran Bandung Jurnal Ilmu dan Teknologi Hasil Ternak Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Animal Production : Indonesian Journal of Animal Production Jurnal Aplikasi Teknologi Pangan Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Aplikasi Teknologi Pangan Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Journal of Tropical Animal Science and Technology Jurnal Sains dan Teknologi Peternakan Jurnal Teknologi Hasil Peternakan Makara Journal of Science Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan Jurnal Agripet Animal Production
Irma Isnafia Arief
Department Of Animal Production And Technology, Faculty Of Animal Science, Institut Pertanian Bogor, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary Other

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Antimicrobial activity of plantaricin IIA-1A5 on whey growth medium againts Pseudomonas aeroginosa: a scanning electron microscopy (SEM) study Mutmainna, Andi; Arief, Irma Isnafia; Lestari, Ayu; Hidayat, Muhammad Nur
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.24 KB) | DOI: 10.33772/jitro.v9i2.22444

Abstract

The study aimed to understand antimicrobial properties of plantaricin derived from L. plantarum IIA-1A5 and its inhibitory mechanism against Pseudomonas aeruginosa ATCC 27853 under SEM (scanning electron microscopy) observation. The inhibition of microbial growth was evaluated according to diameter of inhibitory zone. Scanning electron microscopy was then applied to observe the microstructure. The diameter of antimicrobial activity against Pseudomonas aeruginosa ATCC 27853 reached 6.52 mm in whey medium and 8.45 mm in whey+ medium (added with sucrose, tryptone and yeast extract). The experimental result clearly demonstrated the inhibition Pseudomonas aeruginosa ATCC 27853. Observation by SEM suggested that the antimicrobial activity of Pseudomonas aeruginosa ATCC 27853 related destruction of cellular morphology. The cell membrane was destroyed as indicated by the release of nucleic acid and reduction of membrane potential. In short, our experiment provided meaningful evidence on the future use of plantaricin IIA-1A5 as food preservative through disruption of cellular membrane causing leading to cell dying.Keywords:  Plantaricin, Lactobacillus plantarun, Pseudomonas aeruginosa, antimicrobial activity
Synthesis of Cu2O, Cu2O/Charcoal, and Cu2O/Activated Charcoal Composites as Antibacterial Agents Abidin, Zaenal; Rahmawati, Wahyu Riski; Arief, Irma Isnafia; Rohaeti, Eti
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 4 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.4.564

Abstract

The excessive use of antibiotics to treat bacterial infections can lead to bacterial resistance, necessitating other antibacterial agents as alternatives. This research combined charcoal and activated charcoal with metal oxide, namely copper oxide (Cu2O), which has antibacterial properties against Gram-positive and Gram-negative bacteria, thus producing an antibacterial composite for water treatment processes. Furthermore, this study also examined the effect of different types of sugar as reducing agents on the produced Cu2O and identified the antibacterial activity of Cu2O and its composites. Synthesis of Cu2O through the Tollens-like reaction method using Cu(NO3)2 as a precursor, then adding NaOH, NH4OH, and sugar. The sugars were sucrose, white sugar, and brown sugar. The type of sugar used as a reducing agent affected the size and morphology of the Cu2O produced. Adding Cu2O to charcoal and activated charcoal increased antibacterial properties to charcoal and activated charcoal. Copper oxide, Cu2O/charcoal, and Cu2O/activated charcoal exhibited high antibacterial properties against Escherichia coli (Gram-negative), as of 5.69 ± 0.02 mm and 6.23 ± 0.03 mm, respectively, due to their thinner cell walls compared to Staphylococcus aureus (Gram-positive). The Cu2O synthesized using white sugar as the reducing agent showed the best antibacterial activity, with an 8.26 ± 0.19 mm inhibition zone. Keywords: activated charcoal, antibacterial, charcoal, copper oxide, sugar
Karakteristik Fisikokimia, Kadar Gizi, Organoleptik dan Aktivitas Antioksidan dalam Es Krim Yoghurt Rosela 'Izzati, Farah Diba; Arief, Irma Isnafia; Budiman, Cahyo; Abidin, Zaenal
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 4 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.4.642

Abstract

This study develops roselle yogurt ice cream by combining the probiotic benefits of yogurt with bioactive compounds from roselle flowers. Yogurt is produced through milk fermentation by lactic acid bacteria, while roselle flower extract is rich in anthocyanins and flavonoids known for their antioxidant, antibacterial, and anti-inflammatory properties. The research methods included a comprehensive analysis of physicochemical properties, nutritional content, microbiology, antioxidant activity, and organoleptic characteristics. Using a randomized complete design, data analysis employed ANOVA and the Least Significant Difference tests (P < 0.05), with sensory evaluation conducted using the Kruskal-Wallis test. The results of exploring the synergy between roselle yogurt and ice cream in varying proportions (20:80, 30:70, 40:60) showed that the treatment with a 30:70 ratio of roselle yoghurt (P2) was optimal in physicochemical characteristics such as pH 5.47, total titratable acidity 0.74%, overrun 75.61%, and meltdown rate of 26.98 minutes. P2 also exhibited the highest total antioxidant activity at 137 µg/mL, with good sensory acceptance from consumers. This experiment provided an understanding of the potential of roselle yoghurt ice cream as a product that meets increasingly selective consumers' nutritional and health needs. It is hoped that these findings will stimulate the development of ice cream products that are not only commercially popular but also provide significant health benefits. Keywords: roselle yoghurt ice cream, antioxidant activity, consumer health
The Characteristics of Dried Yoghurt and Market Test on Adolescent Consumers, Adults and Employees in Bogor City Monasdir Monasdir; Irma Isnafia Arief; Zakiah wulandari; Lucia Cyrilla Eko Nugrohowati
ANIMAL PRODUCTION Vol. 25 No. 3 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.3.212

Abstract

This study aimed to analyze the characteristics of dried yogurt, market potential, consumer perception, consumer purchasing behavior, and attributes that affect the purchase of dried yogurt made by teenagers, adults and employees in Bogor City. The results showed that based on testing the characteristics of dried yogurt with parameters of water content, pH, Aw, Viscosity, TAT and total lactic acid bacteria dried yogurt is safe for consumption. Based on the results of the conjoint analysis, it shows that to market dried yoghurt, producers must design dried yoghurt with probiotic content, then the product packaging must be in accordance with consumer tastes, in this case consumers prefer dried yoghurt with a packaging of 7 grams and priced at 10.000.Based on the results of the decriminant analysis, it shows that out of 100 respondents, as many as 90 people will buy dried yogurt if dried yogurt is marketed. Then 4 people will not buy dried yogurt if it is marketed and 6 people are hesitant about the choice. Based on the descriptive results of the study, it was shown that out of 100 respondents there were 47 women and 53 men with large families totaling 4 people, with an average income and expenditure a month of Rp. 500.000 - Rp. 1.500.000.
Evaluasi Good Dairy Farming Practice (GDFP) di Peternakan Sapi Perah Rakyat Kelompok Ternak Mandiri Sejahtera Cijeruk Bogor Iyep Komala; Irma Isnafia Arief; Afton Atabany; Lucia Cyrilla ENSD
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.19650

Abstract

ABSTRACT. Upaya yang dapat dilakukan untuk meningkatkan produksi dan kualitas susu dengan meningkatkan kapasitas SDM peternak dengan melakukan pendampingan untuk penerapan Good Dairy Farming Practices (GDFP). Penelitian ini bertujuan untuk mengevaluasi penerapan GDFP peternakan rakyat dataran tinggi pada Kelompok Ternak Mandiri Sejahtera Cijeruk Bogor. Metode yang digunakan yaitu metode survey dengan wawancara langsung kepada seluruh peternak sapi perah di Peternakan Mandiri Sejahtera sebanyak 22 orang, dengan menggunakan kuisioner yang mengacu kepada Ditjennak (1983) dan FAO (2011) yang dimodifikasi Andriyadi (2012). Berdasarkan karakteristik peternak nilai GDFP paling tinggi terdapat pada peternak dengan umur produktif 21-35 tahun (3.13), tingkat pendidikan D4/S1 (3.2) dan dengan pengalaman beternak 9-15 tahun (3.16). Berdasarkan faktor GDFP, nilai aspek GDFP tertinggi terdapat pada aspek manajemen pakan dan air minum (3.3) dan terendah pada aspek kandang dan peralatan (2.1). Penerapan GDFP pada Kelompok Ternak Mandiri Sejahtera tergolong cukup baik dengan nilai rataan 2.9. Peternak perlu melakukan perbaikan tata laksana pemeliharaan terutama pada aspek cara seleksi, pencatatan usaha, tata letak kandang dan penanganan limbah.(Evaluation of good dairy farming practice (GDFP) in smallholder dairy farms kelompok ternak mandiri sejahtera Cijeruk Bogor)ABSTRAK. Efforts that can be made to increase milk production and quality is by increasing the capacity of the breeders' human resources through providing assistance for the implementation of Good Dairy Farming Practices (GDFP). This study aim is to evaluate the implementation of GDFP on upland people's farms in the Kelompok Ternak Mandiri Sejahtera Cijeruk Bogor. The method used is a survey method with direct interviews to 22 dairy farmers in Mandiri Sejahtera Farms, using a questionnaire that refers to the Ditjennak (1983) and FAO (2011) which is modified Andriyadi (2012). Based on the characteristics of the farmer, the highest GDFP value is found in farmers with productive age of 21-35 years (3.3), education level of Diploma/Bachelor (3.2) and with 9-15 years of livestock experience (3.16). Based on the GDFP factor, the highest GDFP value was found in the feed and drinking water management aspect (3.3) and the lowest was in the cage and equipment (2.1). The implementing of GDFP in the Independent Prosperous Livestock Group is classified good with an average value of 3.0. Farmers need to improve maintenance management, especially in terms of selection, business records, cage layout and waste management.
Karakteristik Mikroorganisme, pH dan Unsur Hara Urin Sapi Perah di Daerah Bogor, Jawa Barat Ely Vebriyanti; Irma Isnafia Arief; Salundik Salundik; Panca Dewi
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.19844

Abstract

ABSTRACT. Penelitian ini bertujuan untuk menganalisis karakteristik mikroorganisme, pH, dan unsur hara pada urin sapi perah sebagai bahan dasar pembuatan biourin di daerah Bogor, Jawa Barat. Sampel penelitian ini adalah urin sapi perah hasil pengambilan urin pada waktu pagi dan sore. Umur sapi perah yang menjadi sampel penelitian adalah 3-4 tahun. Variabel dalam penelitian ini adalah mikroba, bakteri asam laktat (BAL), khamir, bakteri nitrifikasi, pH, NH4, kadar carbon (C), kadar phosfor (P), kadar nitrogen (N), kadar kalium (K), kadar besi (Fe) dam kadar tembaga (Cu). Hasil Penelitian menunjukkan bahwa pengambilan urin sapi pada pagi dan sore tidak pengaruh nyata terhadap total mikroba, total bakteri asam laktat, total kapang khamir, total bakteri nitrifikasi, pH, NH4, kadar phosfor (P), kadar nitrogen (N), kadar kalium (K), kadar besi (Fe) dam kadar tembaga (Cu). Kesimpulan penelitian ini adalah waktu pengambilan urin sapi pada pagi dan sore hari memberikan pengaruh nyata terhadap kadar C namun tidak berpengaruh nyata terhadap kadar K, kadar N, kadar Fe dan kadar Cu. Urin dengan waktu pengambilan pagi hari mengandung kadar C yang lebih tinggi dibandingkan dengan urin pengambilan sore. Hal ini menunjukkan bahwa urin sapi pagi dan sore atau gabungan urin pagi dan sore dapat digunakan sebagai bahan dasar pembuatan biourin sebagai pupuk organic cair.(Characteristics of microorganisme, macro and micro nutrients of dairy cattle urine at Bogor, West Java)ABSTRAK. This study aimed to analyze the characteristics of microorganisme pH and nutrients in the urine of dairy cows as the basic ingredients for making biourin in the Bogor area, West Java. The sample of this research is dairy cow urine with urine collection in the morning and evening. The age of the dairy cows in the research sample was 3-4 years. The parameters in this study were microbes, lactic acid bacteria (LAB), yeasts, nitrifying bacteria, pH, NH4, levels of carbon (C), levels of phosphorus (P), levels of nitrogen (N), levels of potassium (K), levels of iron ( Fe) and copper content (Cu). The results showed that the collection of cow urine in the morning and evening did not have a significant effect on total microbes, total lactic acid bacteria, total yeast molds, total nitrifying bacteria, pH, NH4, , phosphorus (P), levels of nitrogen (N), potassium content (K), iron content (Fe) and copper content (Cu). The conclusion of this study is that the time of taking cow urine in the in the morning and evening has a significant effect on K levels, N levels, Fe levels and Cu levels, urine taken in the morning contains higher levels of C than Urine taken in the afternoon. This shows that morning and evening cow urine or a combination of morning and evening urine can be used as the basic material for making biourine as liquid organic fertilizer.
Performa Pertumbuhan Ayam IPB-D1 pada Perlakuan Pakan dan Manajemen Pemeliharaan yang Berbeda Muhammad Fikri Al Habib; Sri Murtini; Luci Cyrilla; Irma Isnafia Arief; Rita Mutia; Cece Sumantri
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.16375

Abstract

ABSTRAK. Pelepasan ayam IPB-D1 sebagai rumpun baru yang disebar di masyarakat maka sistem pemeliharaannya secara umum bersifat ekstensif dan pakan yang diberikan memanfaatkan pakan lokal. Tujuan penelitian ini adalah untuk mengetahui performa ayam IPB-D1 dengan perlakuan pemeliharaan P1 (sistem kandang Closed House dan penggunaan 100% pakan komersil) serta perlakuan P2 (sistem kandang umbaran dan pencampuran pakan lokal hingga 70%). Penelitian ini menggunakan ayam IPB-D1 berjumlah 503 ekor DOC yang dipelihara hingga umur 12 minggu. Performa ayam diukur berdasarkan bobot badan, laju pertumbuhan, konsumsi pakan, konversi pakan serta tingkat kematian ayam pada umur DOC-12 minggu. Data dianalisis secara deskriptif dengan menyajikan rataan, simpangan baku, koefisien keragaman dan analisis statistik menggunakan uji T. Hasil penelitian performa ayam IPB-D1 pada perlakuan P1 diperoleh bobot badan jantan 1378.2694.56 g dan betina 1178.33119.33 g, sedangkan pada perlakuan P2 diperoleh bobot badan jantan 1173.8260.1 g dan betina 957201.7 g. Laju pertumbuhan pada kedua perlakuan bersifat positif, serta konversi pakan pada perlakuan P1 (2.88) dan P2 (3.44) cukup baik. Kesimpulan penelitian ini adalah ayam IPB-D1 diperoleh performa yang sangat baik pada perlakuan P1 dan mampu beradaptasi pada perlakuan kandang sistem umbaran serta mampu memanfaatkan campuran pakan lokal yang baik pada perlakuan P2. (The growth performance of IPB-D1 chickens in different feed treatments and production systems) ABSTRACT. The release of the IPB-D1 chicken as a new breed that is spread in the community, the production system is generally extensive and the feed provided utilizes local feed. The purpose of this study was to determine the performance of IPB-D1 chicken with production treatment P1 (Closed House cage and use of 100% commercial feed) and P2 treatment (outdoor cage and use local feed mixing up to 70%). This study used 503 DOC IPB-D1 chickens which were maintained until 12 weeks of age. Chicken performance was measured based on body weight, growth rate, feed consumption, feed conversion and chicken mortality at 1-12 weeks. Data were statistically analyzed using the T test and descriptive analysis with means, standard deviations, and coefficient of diversity. The results of the study of the performance of IPB-D1 chickens in P1 treatment obtained male body weights 1378.26 94.56 g and females 1178.33 119.33 g, while in P2 treatment obtained male body weights 1173.8 260.1 g and females 957 201.7 g. Growth rates in both treatments were positive, and feed conversion was good in P1 treatments (2.88) and P2 treatments (3.44). The conclusion is that the IPB-D1 chicken obtained a very good performance in the P1 treatment and was able to adapt to the treatment of outdoor cages and was able to utilize the local feed mixture in the P2 treatment.
Kinerja Produksi dan Kualitas Telur Ayam Petelur yang Diberi Minum Air Gambut dan Air Non Gambut Deni Fitra; Niken Ulupi; Irma Isnafia Arief; Rita Mutia; Luki Abdullah; Sadarman Sadarman; Apriadi Pasaribu; Guslian Abdul Basir
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.15802

Abstract

ABSTRAK. Penelitian ini bertujuan untuk mengevaluasi kinerja produksi dan kualitas telur ayam petelur yang diberi air gambut (AG) dan air non gambut (ANG). Penelitian ini menggunakan 30 ekor ayam petelur strain lohman brown umur 15 bulan dengan pemberian pakan ransum komersial. Penelitian terdiri dari dua kelompok ayam dengan jumlah masing-masing 15 ekor. Kelompok pertama diberi minum AG dan kelompok kedua diberi ANG. Peubah yang diamati adalah konsumsi air minum, konsumsi ransum, produksi dan massa telur, konversi ransum, bobot telur, indeks putih telur, indeks kuning telur, tebal cangkang telur dan nilai haugh unit. Data penelitian dianalisis dengan uji-t untuk mengetahui perbedaan antar kelompok perlakuan. Hasil penelitian menunjukkan bahwa konsumsi air minum, konsumsi ransum dan konversi ransum secara statistik berbeda nyata. Konsumsi air minum pada perlakuan AG (286,57 ml/ekor/hari) lebih tinggi dari pada perlakuan ANG (264,24 ml/ekor/hari), demikian juga konsumsi ransum (98,82 g/ekor/hari vs 90,10 g/ekor/hari). Sedangkan konversi ransum pada perlakuan AG (2,71) lebih baik dari pada perlakuan ANG (3,18). Pada peubah kualitas telur hanya bobot telur yang menunjukkan perbedaan nyata. Bobot telur pada perlakuan AG (55,85 g/butir) lebih tinggi dari pada perlakuan ANG (54,18 g/butir). Kesimpulan dari penelitian ini adalah pemberian minum dengan AG mampu memperbaiki kinerja produksi ayam petelur, terlihat dari konsumsi air minum, konsumsi dan konversi ransum. Sedangkan pada kualitas telur hanya terlihat dari bobot telur. (Performance and egg quality of laying hen given peat water and non peat water) ABSTRACT. The aim of this study was to evaluate the performance and egg quality of laying hens given peat water (PW) and non-peat water (NPW). This study used 30 laying hens strain lohman brown aged 15 months with commercial ration feeding. The study consisted of two groups of chickens with 15 chickens each. The first group was given PW and the second group was given NPW. The variables observed were water consumption, feed consumption, egg production and mass, FCR, egg weight, albumin and yolk index, egg shell thickness and haugh unit value. Data obtained were analyzed using t-test to determine the differences between treatment groups. The results showed that water consumption, feed consumption and FCR were statistically difference. The daily water consumption in PW treatment (286.57 ml/bird/day) was higher than in NPW treatment (264.24 ml/bird/day), as was ration consumption (98.82 g/bird/day vs 90.10 g/ bird/day). While the ration conversion in PW treatment (2.71) was better than that of NPW treatment (3.18). In the egg quality variable, only egg weight showed a significant difference. Egg weight in PW treatment (55.85 g/egg) was higher than in NPW treatment (54.18 g/egg). The conclusion of this experiment was the given peat water to laying hens was able to improve the production performance, indicated by water consumption, feed consumption and FCR. Meanwhile, the quality of eggs can only be seen from the egg weight.
Expression of Lon-like Protease Gene from Lactobacillus plantarum IIA-1A5 in Escherichia coli BL21(DE3) Olfa Mega; Cece Sumantri; Irma Isnafia Arief; Cahyo Budiman
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14904

Abstract

Proteases are one of most important and abundant enzymes produced by the biotechnology industry, for scientific, physiological and industrial application and dominates of the whole enzyme market. Lactobacillus plantarum IIA-1A5 is an Indonesian lactic acid bacteria (LAB) isolated from beef Peranakan Ongole cattle. Preliminary analysis on its whole genome sequence indicated that this strain harbours some genes involved in protein degradation and might be promising to be further applied. This study aims to optimize the gene sequence of a lon-like protease of L. plantarum IIA-1A5 for heterologous expression system. The Lon-like gene expression system is made using genes that have been optimized first in silico. pET-28a(+), E. coli BL21(DE3), Nde1 and BamH1 were used in this study as a expression vector, a host and retriction enzyme, respectively. Molecular weight was validated using SDS-PAGE and expasy.org software. The results showed that optimization increased codon adaptation index value (CAI) and GC content to 0.97 and 56.57%, respectively, which were suitable for the E. coli expression system. The Lon-like IIA gene was successfully expressed in the cell cytoplasm by induction of 1 mM Isopropyl -D-1-thiogalactopyranoside (IPTG) at 37 C. As many as 88% of Lon-like IIA codons were distributed in the 91-100 quality group. Lon-like IIA was successfully expressed in a host cell induced with 1 mM IPTG at 37oC . IPTG induction was performed at the 3rd hour of incubation with OD600 0.59. In addition, Lon-like IIA molecular weight was detected approximately 43 kDa.
Pertumbuhan dan Produksi Bakteriosin Lactobacillus fermentum Asal Dangke pada Media Whey Dangke Rajmi Faridah; Epi Taufik; Irma Isnafia Arief
Jurnal Agripet Vol 17, No 2 (2017): Volume 17, No. 2, Oktober 2017
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v17i2.8104

Abstract

ABSTRAK.Dangke merupakan makanan khas Enrekang, Sulawesi Selatan. Dangke menghasilkan hasil sampingan yang disebut whey. Komponen nutrisi yang terkandung dalam whey dapat digunakan oleh bakteri asam laktat (BAL) untuk pertumbuhannya. Salah satu BAL yang dapat memproduksi bakteriosin yaitu Lactobacillus fermentum (L. fermentum). Strain L. fermentum asal dangke, yang digunakan dalam penelitian yaitu A323L, B323K, dan C113L. Hasil penelitian menunjukkan bahwa fase logaritmik dari L. fermentum strain A323L yaitu pada waktu inkubasi 24-28 jam, sedangkan strain B323K dan C113L pada waktu inkubasi 20-24 jam. Zona hambat dari semua strain L. fermentum termasuk kategori lemah pada media pertumbuhan whey dangke, tetapi strain C113L mempunyai daya hambat terbaik.(The Growth and Production Bacteriocin of bacteria Lactobacillus fermentum using dangke whey as medium)ABSTRACT. Dangke is a local dairy product of Enrekang, South Sulawesi. Dangke processing produced a by-product called whey. Nutritional components in whey can be utilized by lactic acid bacteria (LAB) as a nutritional source of growth. One of bacteriocin producing lactic acid bacteria is Lactobacillus fermentum (L. fermentum). L. fermentum strain isolated from dangke, which used in this research were A323L, B323K, and C113L. The results showed that logarithmic phase of L. fermentum strain A323L were occurred at the incubation time of 24-28 hours, whilst strain B323K and C113Lwere at 20-24 hours. Inhibition zone of all strain of L. fermentum was categorized as weak in whey dangke medium, but strain C113L was the best among them.
Co-Authors 'Izzati, Farah Diba . TRIVADILA Afton Atabany Agpretasia, Yodilla Ahmad Furqon Alfiansyah, Teuku Muhammad Alifiya, Qorina Amalina Nur Wahyuningtyas Ambar Mudigdo Amelia Friska Pertiwi Anas Qurniawan Andi Febrisiantosa Andi Nurul Mukhlisah Andri Kusmayadi Angelina, Putri Nur Apriadi Pasaribu Apriantini, Astari Aristo Kurniawan Sio Astari Apriantini Asti Yosela Oktaviana AYU LESTARI Bagus Priyo Purwanto Bahri Syamsuryadi Betty S. L. Jenie Cahyo Budiman Cece Sumantri Damiana Rita Ekastuti Darmawati, Maulita Putri Detiara, Azzahra Dondin Sajuthi Dono Indarto Dwi Febiyanti - Edi Erwan Ekajayanti Kining El Latifa Sri Suharto Epi Taufik Erlita Sari, Pipit Eti Rohaeti Fajri, Aulia Irhamni Faridah, Rajmi Febrina, Bunga Putri Firmansyah Firmansyah Fitra, Deni Fitri M Manihuruk Fitriananda, Putri Ghaling Achmad Guslian Abdul Basir Hajrawati Hajrawati Hanifah Nuryani Lioe Henny Nuraini Ida Ayu Putu Sri Widnyani Irmanida Batubara Isyana Khaerunnisa Isyana Khaerunnisa Iyep Komala J. Hermanianto Jakaria Jakaria Jaya Putra Jahidin Kamila, Elfa Aida Lilis Nuraida Luci Cyrilla Lucia Cyrilla Eko Nugrohowati Lucia Cyrilla Eko Nugrohowati Lucia Cyrilla ENSD Lucia cyrilla ENSD Lucya Cyrilla Eko Nugrohowati Supriyadi Dekriyatna Luki Abdullah MADE ASTAWAN Mahmud, Andi Tenri Bau Astuti Maulitia, Ziyan Tirta Mauludi, Muhammad Rifqi Mochammad Sriduresta Soenarno Monasdir Monasdir Monasdir, Monasdir Muh. Achyar Ardat Muhamad Arifin Muhamad Arifin, Muhamad Muhamad Syukur Muhammad Fikri Al Habib Muhammad Nur Hidayat Muhammad Yusuf Murtini, Devi Mutmainna, Andi Ni Wayan Triwulandhari Niken Ulupi Ninuk Purnaningsih Noraimah binti Sulaiman Noraimah Binti Sulaiman Noraimah Binti Sulaiman Nur Afni Nurul Hidayati Olfa Mega Panca Dewi Putra Jahtra Berutu R A Maheswari R. R. A. Maheswari Rahmawati, Wahyu Riski RIFKHAN RIFKHAN Rita Mutia Rudi Afnan Sadarman Sadarman Saiful Anwar Salsabila, Haditsah Salundik Salundik . Sari, Pipit Erlita Sena, Anantha Septi Dwi Utami Setyo Pertiwi Silaban, Yosua Kristianto Siti Aminah Sitta Fitri Rahmadhina Soenarno, M. Sriduresta Soenarno, Moch. Sriduresta sri murtini . Sri Rahmatul Laila Sri Sugiarti Suharyanto Suharyanto Suharyanto Suharyanto Sulassih, . Syah, Setiawan Putra Tjut Awaliyah Zuraiyah Tuti Rostianti Maulani Tuti Suryati Tutik Wresdiyati Vebriyanti, Ely Yandra Arkeman Yantyati Widyastuti Yeni Setiorini Yeni Setiorini Yong Soo Kim Yusuf Sumaryana Zaenal Abidin Zakiah Wulandari