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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Partner ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production Indonesian Journal of Agricultural Research ARSHI Veterinary Letters Sainstek : Jurnal Sains dan Teknologi Zoo Indonesia Livestock and Animal Research Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Bambang Purwantara
Division Of Reproduction And Obstetrics, Department Of Veterinary Clinic, Reproduction, And Pathology, IPB University, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : Media Veteriner

 1 
The Influence of Homologous and Heterologous Sera in Culture Medium on in Vitro Maturation and Fertilization of Sheep Oocytes Ita Djuwita; Yohan Rusyiyantono; Kusdiantoro Muhamad; Bambang Purwantara; Yuhara Sukra
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The influence of various types of sera i.e. Fetal Calf Serum (FCS), Bovine Post Estrous Serum (SPES) as heterolog sera; and Ewes Fetal Serum (EFS) and Ewes Serum (ES) as homologue sera on in vitro maturation and fertilization of sheep oocytes was investigated using Tissue Culture Medium 199TM (TCM 199. Gibco). Sovine sera were collected from cow at seven days post-estrus, while ewes sera were collected during estrous (ES-HO) and six days post estrous (ES-H6). The result shows that the maturation rate of oocytes cultured in medium supplemented with EFS, ES-HO and ES-H6 at  concentrations of 32.9%, 68.7%, and 67.6% respectively is better than those supplemented with FCS and SPES at concentrations of 22.2% and 28.9%, respectively. In vitro fertilization rate were significantly higher in medium supplemented with ES-HO and ES-H6 at concentration of 65.4% and 65.8% (P < 0.05) than that supplemented with SPES at concentration of 31.7%. In conclusion, ewes sera could be used in culture medium for promoting both in vitro maturation and fertilization rate of sheep oocytes.
Study on the Application of Human Chorionic Gonadotropin (hCG) in Dairy Cow Superovulated with Pregnant Mare Serum Gonadotropin - Monoclonal Antibody (PMSG-MoAb) Anti PMSG Iman Supriatna; Tuty Laswardi Yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 5 No. 2 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The research was planned to yield the biologic potential synergism of MoAb-hCG in increasing the embryo number. Twenty five dairy cows were divided into five groups. The Control Group was superovulated with 2,500 IU PMSG intramuscularly (i.m.), the remaining of the Group II, III, IV and V consecutively after being superovulated by 2,500 IU PMSG were treated with MoAb intravenously (i.v.), hCG i.v., MoAb-hCG i.v. and MoAb i.v-hCG i.m. Data evaluation were analyzed using ANOVA and LSD test. The result showed that there were increasing in number of transferable embryo per donor produced (P<0.01) after application with either MoAb i.v. (9.6), hCG i.v. (9.4), MoAb-hCG i.v. (11.2), or MoAb i.v.-hCG i.m. (9.6). The Control Group produced only 2.4 embryo per donor. The combined MoAb and hCG in one treatment were not able to increase the embryo yield (P>0.05).Key words: MoAb, PMSG, hCG, superovulation, donor, embryo
The Use of Direct Transfer Method on Embryo Cryopreservation in Dairy Cattle Iman Supriatna; Tuty Laswardi yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 6 No. 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The experiment was carried out to study the use of direct transfer method in embryo cryopreservation by using two cryoprotectants and the effectivity of various concentrations of sucrose during cryoprotectant removal. Eighty-fourmorula stage embryos were divided equally into two groups and were treated by using 1.5 M ethylene glycol (EG) and 1.5 M 1,2-propanediol as cryoprotectant. The embryos were frozen using programmable embryo freezing machine on step by step decreasing temperature. Frozen embryos were thawed and cryoprotectants were removed either without sucrose (0 M) or with sucrose in concentration of 0.2 M, 0.4 M and 0,8 M. The results showed that the quality of the thawed embryos cryopreserved using 1.5 M EG was better than that using 1.5 M PROH. The survival rate on the embryos cryopreserved with 1.5 M EG (92.8%) was higher than 1.5 M PROH (78.6%) (P0.05). The viability of embryos exposed to 0 M, 0.2 M, 0.4 M and 0.5 M sucrose were 80.0, 80.8, 90.9 and 81.80% respectively. In contrast, by using 1.5 M PROH, rehydration with 0.4 M (83.3%) and 0,8 M (90.0%) sucrose was significantly better compared to those without (22.2%) or with 0.2 M (36.3%) sucrose (p
The experiments were carried out to study the influence of different sera on in vitro maturation and early development of ovine embryos. Sera used in this study were FLS (Fetal Lamb Serum), ewe serum collected on Day 0 (ES-DO) and Day 6 (ES-D6) of oestrus cycles. Ovine oocyte were matured and cultured in TCM-199 supplemented with 10 % of different sera. Results of this experiment showed that supplementation of ES-DO or ES-D6 could support maturation rate (Metaphase-II) at 68.7% and 67.6%, respec Yohan Rusiyanto; Bambang Purwantara; Yuhara Sukra
Media Veteriner Vol. 7 No. 1 (2000): Media Veteriner
Publisher : Media Veteriner

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Abstract

The experiments were carried out to study the influence of different sera on in vitro maturation and early development of ovine embryos. Sera used in this study were FLS (Fetal Lamb Serum), ewe serum collected on Day 0 (ES-DO) and Day 6 (ES-D6) of oestrus cycles. Ovine oocyte were matured and cultured in TCM-199 supplemented with 10 % of different sera. Results of this experiment showed that supplementation of ES-DO or ES-D6 could support maturation rate (Metaphase-II) at 68.7% and 67.6%, respectively better than FLS (32.9%). The fertilization rate was significantly higher (p
Co-Authors . Paggi ACHMAD FARAJALLAH Agus O . Sudradjat Ahmad Furqon Amrozi Anak Agung Gede Sugianthara Anna Grace Holliman Anna Nabilla Anny Amaliya Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Asep Kurnia Ashley Lorraine Greene Austin William Klein Bambang Hadisutanto Bambang Hadisutanto Bethany Peyton Haynes BINTAR, ANIFA Briana Lashun Burkes Brianna Nicole Palmer Brittany Renee Franks Cece Sumantri Chairdin Dwi Nugraha, Chairdin Dwi Cobie Rutherford Cody Triston Lore Courtney Ann Lehmann Deddy Muchtadi DEDI CANDRA Diana Andrianita Kusumaningrum Dondin Sajuthi Dwi Sendi Priyono Ed Dechert Ekayanti Mulyawati Kaiin Elma Yuliani Yessa Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Erdogan Memili Faisal Amri Satrio Fajar Basuki Farajallah, Ahmad fatmila, dian tria Fransiska R Zakaria Fuad Hasan Gholib Gholib Gholib Gholib, Gholib Gono Semiadi GONO SEMIADI Gozali Moekti Grace Jennette Llyod GREVE, TORBEN H0IER, RENE Hadi S Alikodra Haleigh Margaret Giles Hannah Faith Stepp Hannah Marie Miller Heistermann, Michael HERA MAHESHWARI Herdis . Herdis Herdis Herwijanti, Enniek Hery Widijanto HERY WINARSI Ietje Wientarsih IETJE WIENTARSIH Iman Supriatna Irida Novianti Ita Djuwita ITA DJUWITA James Dean Smith Jasmyn Elaine Fearon Jeneva Ruth Seidl John Mark Kirby Kaiin, Ekayanti Mulyawati Katie Yvonne Sanford Ketut Sutama Kipp Brown Kurnia, Asep Kusdiantoro Mohamad Laura Marie Schaeffer Lauren Susanne Blalock Lies Parede Hernomoadi Lindsey Virginia Pansano LUTHFIRALDA SJAHFIRDI M Agus Setiadi M Imron M. Zairin Junior MADE ASTAWAN Michael Heistermann Mildred Elizabeth Aldridge Mokhamad Fakhrul Ulum, Mokhamad Mozes R . Toelihere Mozes R. Toelihiere Mozes R. Tolihere Muhamad Gazali MUHAMMAD AGIL Muhammad Danang Eko Yulianto Muhammad Imron Naseer Ahmad Kutchy Ni Wayan Kurniani Karja Nurgiartiningsih, V. M. A. Nurul Taufiqu Rochman Nurul Taufiqu Rochman Olivia Lucienne Cohn-White Oloan Parlindungan Oriza Savitri Ariantie Oriza Savitri Ariantie Pardede, Berlin Pandapotan Polmer Situmorang Polmer Z. Situmorang Pudji Astuti Pudji Astuti Putri, Rafika Febriani R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Rachel Rebecca Scott Reviany Widjajakusuma Reviany Widjajakusuma Rhesti Indriastuti RIKA RAFFIUDIN Riley Dawn Hanby Ristika Handarini Rosyada, Zulfi Nur Amrina Roza Helmita Ryan Matthew Pickle Santoso . Santoso Santoso SCHMiDT, METTE Siti Darodjah Siti Darodjah Rasad Sri Darwati SRI RAHAYU Surya Natal Tambing Surya Natal Tambing Sussi Astuti Sutanto, Wahyudi Himawan Sutarman Muhardja Suyadi Suyadi TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Tri Harsi Tuti L. Yusuf Tutik Wresdiyati Tuty L . Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tyler B. McMurray WIDJAJA, M. CHANDRA Wike andre Septian William Wess Anglin Wilmientje Marlene Mesang Nalley Yanyan Setiawan Yessa, Elma Yuliani Yohan Rusiyanto Yudi Yuhara Sukra Yuni Siswani Zachary Vinz Moody