Article Per Year (5 Year)
p-Index From 2021 - 2026
4.299
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioma : Berkala Ilmiah Biologi Jurnal Pendidikan Matematika dan IPA AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Medical Journal of Indonesia Biology, Medicine, & Natural Product Chemistry Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Indonesian Food and Nutrition Progress Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Penelitian Perikanan Indonesia Biota Jurnal Biomedika dan Kesehatan Borneo Journal of Medical Laboratory Technology Transformatif: Jurnal Pengabdian Masyarakat Berkala Ilmiah Biologi
Ardaning Nuriliani
Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada Jl. Teknika Selatan, Sekip Utara, Yogyakarta, Indonesia, 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

Published : 34 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Effect of Black Rice Bran ‘Sembada Hitam’ on T47D Breast Cancer Cells: Effect of Black rice bran on T47D Cios Conara , Flafiani; Oktavya, Galuh; Asih Purwestri, Yekti; Nuriliani, Ardaning
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.17

Abstract

Indonesians have a rich tradition of incorporating black rice into their diet as a functional food due to its high antioxidant content. This research investigates the effects of the ethanolic extract of "Sembada Hitam" black rice bran on T47D breast cancer cells, specifically its cytotoxic, cell growth, and apoptotic induction properties. The MTT assay method was used to evaluate the cytotoxicity of the black rice bran extract on T47D cells after 24 and 48 hours of incubation. The acridine orange/propidium iodide (AO/PI) dye double staining method was employed to assess the apoptosis-inducing properties of the extract. Additionally, a cell growth assay was conducted to evaluate the effect of the extract on cell growth, with observations recorded on days 0, 3, and 6. The results revealed that the ethanolic extract of black rice bran "Sembada Hitam," when administered at various concentrations ranging from 7.81 to 1000 µg/mL, did not exhibit cytotoxic effects on T47D cells during the 24 and 48 hours of incubation. The cell growth assay revealed that T47D cells treated with the ethanolic extract of "Sembada Hitam" black rice bran at concentrations of 250 and 500 µg/mL exhibited lower growth rates than the 1% DMSO group from the 3rd to 6th day after incubation. The ethanolic extract of "Sembada Hitam" at concentrations of 250, 500, and 1000 µg/mL resulted in cell death of 10.64 ± 2.98%, 9.99 ± 5.87%, and 5.84 ± 0.78%, respectively. In conclusion, this study found that the ethanolic extract of "Sembada Hitam" black rice bran did not demonstrate significant cytotoxic effects on T47D breast cancer cells and is, therefore, unlikely to be a suitable candidate for an anti-cancer agent.
Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation: Black rice bran ‘Sembada Hitam’ protect cytotoxicity of H2O2 Aprilianti, Suci Hari; Purwestri, Yekti Asih; Saragih, Hendry T.S.S.G; Nuriliani, Ardaning
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.01

Abstract

Oxidative stress induced by hydrogen peroxide (H2O2) can lead to cellular damage, contributing to degenerative diseases and aging. Black rice bran is a functional food known for its antioxidant properties, which are crucial in reducing the adverse effects of oxidative stress and maintaining redox balance. In this study, we aimed to investigate the protective effect of the extract of black rice bran (EBRB) 'Sembada Hitam' on Vero cells against H2O2 toxicity. To evaluate the protective effect, a co-culture method was employed, and cell viability was assessed using the MTT assay. Additionally, cell growth was examined through trypan blue staining. Vero cells were exposed to different concentrations of H2O2 and EBRB for a 24-hour period. The results demonstrated that EBRB at concentrations of 15.625, 250, and 500 μg/mL exhibited a protective effect on Vero cells exposed to H2O2 at concentrations of 100, 200, and 400 μM, respectively. Notably, when Vero cells were treated with EBRB at concentrations of 250 or 500 μg/mL for five days in conjunction with H2O2 exposure at concentrations of 200 or 400 μM for 24 hours, a significant decrease in cell viability was observed on day 3. Based on the collective findings, it can be concluded that EBRB has the potential to protect Vero cells against H2O2 -induced toxicity, but primarily during a short-term incubation period. Overall, this study highlights the protective properties of EBRB against H2O2 -induced cellular damage and emphasizes the importance of further investigations to fully elucidate the underlying mechanisms and potential long-term effects of EBRB on cell viability.
Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation: Black rice bran ‘Sembada Hitam’ protect cytotoxicity of H2O2 Aprilianti, Suci Hari; Purwestri, Yekti Asih; Saragih, Hendry T.S.S.G; Nuriliani, Ardaning
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.01

Abstract

Oxidative stress induced by hydrogen peroxide (H2O2) can lead to cellular damage, contributing to degenerative diseases and aging. Black rice bran is a functional food known for its antioxidant properties, which are crucial in reducing the adverse effects of oxidative stress and maintaining redox balance. In this study, we aimed to investigate the protective effect of the extract of black rice bran (EBRB) 'Sembada Hitam' on Vero cells against H2O2 toxicity. To evaluate the protective effect, a co-culture method was employed, and cell viability was assessed using the MTT assay. Additionally, cell growth was examined through trypan blue staining. Vero cells were exposed to different concentrations of H2O2 and EBRB for a 24-hour period. The results demonstrated that EBRB at concentrations of 15.625, 250, and 500 μg/mL exhibited a protective effect on Vero cells exposed to H2O2 at concentrations of 100, 200, and 400 μM, respectively. Notably, when Vero cells were treated with EBRB at concentrations of 250 or 500 μg/mL for five days in conjunction with H2O2 exposure at concentrations of 200 or 400 μM for 24 hours, a significant decrease in cell viability was observed on day 3. Based on the collective findings, it can be concluded that EBRB has the potential to protect Vero cells against H2O2 -induced toxicity, but primarily during a short-term incubation period. Overall, this study highlights the protective properties of EBRB against H2O2 -induced cellular damage and emphasizes the importance of further investigations to fully elucidate the underlying mechanisms and potential long-term effects of EBRB on cell viability.
Effect of Black Rice Bran ‘Sembada Hitam’ on T47D Breast Cancer Cells: Effect of Black rice bran on T47D Cios Conara , Flafiani; Oktavya, Galuh; Asih Purwestri, Yekti; Nuriliani, Ardaning
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.17

Abstract

Indonesians have a rich tradition of incorporating black rice into their diet as a functional food due to its high antioxidant content. This research investigates the effects of the ethanolic extract of "Sembada Hitam" black rice bran on T47D breast cancer cells, specifically its cytotoxic, cell growth, and apoptotic induction properties. The MTT assay method was used to evaluate the cytotoxicity of the black rice bran extract on T47D cells after 24 and 48 hours of incubation. The acridine orange/propidium iodide (AO/PI) dye double staining method was employed to assess the apoptosis-inducing properties of the extract. Additionally, a cell growth assay was conducted to evaluate the effect of the extract on cell growth, with observations recorded on days 0, 3, and 6. The results revealed that the ethanolic extract of black rice bran "Sembada Hitam," when administered at various concentrations ranging from 7.81 to 1000 µg/mL, did not exhibit cytotoxic effects on T47D cells during the 24 and 48 hours of incubation. The cell growth assay revealed that T47D cells treated with the ethanolic extract of "Sembada Hitam" black rice bran at concentrations of 250 and 500 µg/mL exhibited lower growth rates than the 1% DMSO group from the 3rd to 6th day after incubation. The ethanolic extract of "Sembada Hitam" at concentrations of 250, 500, and 1000 µg/mL resulted in cell death of 10.64 ± 2.98%, 9.99 ± 5.87%, and 5.84 ± 0.78%, respectively. In conclusion, this study found that the ethanolic extract of "Sembada Hitam" black rice bran did not demonstrate significant cytotoxic effects on T47D breast cancer cells and is, therefore, unlikely to be a suitable candidate for an anti-cancer agent.
Co-Authors Abdul Rachman Adania, Baik Aisyah Adaninggar, Ascarti Adi Susanto Adi Susanto Adi Susanto Aeniah, Siti Agus Hermawan Allimi, Hayu Swari Almunawar, Muhammad Fikri Andi Mahendra Andriyani, Eka Fitri Siti Anisya, Alifia Anita Restu Puji Raharjeng Aprilianti, Suci Hari Aprilianti, Suci Hari Utami Apriliyani, Tia Ariesti, Wiwin Arif Luthfi Nurul Huda Artik, Arba' Ramadhani Ascarti Adaninggar Ashfiya Hanif Hasnadewi Asih Purwestri, Yekti Aswi A. Rofiqoh Bambang Retnoaji Bima Mahendra Bima Mahendra Bima Mahendra Cios Conara , Flafiani Conara, Flafiani Cios Della Blatama Empra, Desi Eka Putri Fajar Sofyantoro Faulana, Arum Fahmi Firdiana, Vykra Aulia Flafiani Cios Conara Flafiani Cios Conara Flafiani Cios Conara Gunanegara, Rimonta Febby Halim, Shuha Ma’muriyah Haris Setiawan Hayu Swari Alimi Hendry Saragih Hendry Saragih Hendry Saragih Hendry T.S.S.G. Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti SG Saragih Hidayah, Lailly Tsania Nur Hidayat, Rheina Faticha Asyamsa Ibnu Agus Ariyanto Jessy Octavia Harahap Johanes Sugiyanto Karlina, Ina Karmilah, Karmilah Laili Mufli Zusrina Lailly Tsania Lailly Tsania Nur Hidayah Lailly Tsania Nur Hidayah Larasshita Putri Adhitya Sani Lisna Hidayati, Lisna Luthfi Nurhidayat Maharesi, Chesa Ekani Manesta Edelweis Jingga Mataram, Made Bagus Auriva Mei Ria Santi Mulyati Mulyati Murtiadi Erlan Supraitno Mutiyani, Novita Nastiti Wijayanti Nasution, Nurlita Putri Bela Ni Wayan Erly Sintya Dewi Nurhidayah, Septika Nurhidayat, Luthfi Oktaviana, Shintia Oktavya, Galuh Prasasti, Filosofia Florista Tesla Aulia Pratiwi, Arini Dian Primahesa, Alfian Pusparini, Nur Ainun Oktavia Puteri, Nabila Ramiza Putri, Raden Roro Risang Ayu Dewayani Rahayu, Fadiah Sri Rahmadian, Desti Rarastoeti Pratiwi Retno Wulandari Ria Vena Septhay Sari Roesma Narulita Rohmah, Zuliyati Ruth Liananda Citra Dolok Saribu Sadiman Sadiman Saeed, Faisal Sandi Francisco Pratama Saragih, Hendry Saragih, Hendry T.S. Saragih, Hendry T.S.S.G Saragih, Hendry Tri Sakti SG Sari, Dini Wahyu Kartika Saribu, Ruth Liananda Citra Dolok Sari’ah Cintami Damayanti Septriani, Nur Indah Septriani, Nur Indah Setyo, Agnessia Asian Budi Shalihah, Fianicha Siwi Dwi Rahayu Slamet Widiyanto Sudarsi, Nurini Aru Sunarti Susilo Hadi, Susilo Syam, Adi Mazdi Tia Apriliyani Utami, Zidni Meirizka Wardani, Ester Dewanti Yovita Woro Anindito Sri Tunjung Yekti Asih Purwestri Yuny Erwanto Yuvita, Arneta Zeyna Listy Zulfatin Zuliyati Rohmah Zusrina, Laili Mufli