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AKTIVITAS ANTIFUNGI EKSTRAK METANOL DAUN SALAM (Syzygium polyanthum [Wight] Walp.) TERHADAP PERTUMBUHAN Malassezia sp. (M1) SECARA IN VITRO Sulistrioningsih, Sulistrioningsih; Wardoyo, Elvi Rusmiyanto Pancaning; Kurniatuhadi, Rikhsan
Jurnal Protobiont Vol 9, No 2 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i2.45849

Abstract

Malassezia sp. is a normal flora on the skin of humans and animals. A considerable amount of the fungus may cause tinea versicolor and dandruff. One of the plants that is known to have antifungal compounds is bay leaf (Syzygium polyanthum [Wight] Walp.). This research was done to determine the ability and the Minimum Inhibitory Concentration (MIC) of methanol extract from bay leaves S. polyanthum in inhibiting the growth of Malassezia sp. (M1). This study used a completely randomized design (CRD) with  treatment levels that consisted of concentrations of 80%, 90%, and 100%, negative control (sterile distilled water) and positive control (ketoconazole 2%). The antifungal activity test was carried out using the Kirby-Bauer method. The data showed that each treatment levels had different results. Concentrations of 90% and 100%, and positive control showed inhibition zones of 9.27 mm, 11.57 mm, and 16.67 mm, respectively. Therefore the concentration of 90% was the Minimum Inhibitory Concentration (MIC) of methanol extract from bay leaves S. polyanthum against the growth of Malassezia sp. (M1).
Pertumbuhan Stek Batang Nepenthes reinwardtiana Miq. Setelah Direndam Dengan Urin Kambing Elvi Rusmiyanto Pancaning Wardoyo, Sri Mufiddah, Mukarlina,
Jurnal Protobiont Vol 7, No 1 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i1.23613

Abstract

Nepenthes reinwardtiana Miq. is one of the species of Nepenthes found in west Borneo where its population in nature is declining. Ex-situ conservation efforts by cultivating vegetatively through stem cuttings need to be done to overcome the extinction of N.reinwardtiana. One of the factors that determines the growth of cuttings is the content of the external growth regulator namely auxin. One of the sources of organic auxin that can be used is goat urine. This research was conducted to find out the effects of goat urine on the growth of stem cuttings of N.reinwardtiana and to find out the concentration of goat urine that provides the best growth on N.reinwardtiana stem cuttings. The method used is completely randomized design with 4 treatments which is goat urine concentration of 0,15,30 and 40% , each treatment is repeated 3 times so that 12 units of experiment were obtained. The results show that immersion of goat urine has significant effect on the time of shoot, the number of roots and height of the plant but does not provide the number of shoots. Goat urine concentration of 15% produces that fastest shoot growth namely 11.23 days while the concentration of 30% gives the best result on the number of roots as many 2 pieces and the height of the plant namely 4.17 cm.
Inventarisasi Jenis Paku-Pakuan (Pteridophyta) di Hutan Sebelah Darat Kecamatan Sungai Ambawang Kalimantan Barat Elvi Rusmiyanto P.W., Hotmatama Hasibuan, Rizalinda,
Jurnal Protobiont Vol 5, No 1 (2016)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v5i1.14883

Abstract

Ferns (Pteridophyta) as a plant community in biodiversity has important ecological function in forest ecosystems and human living. The human activities on the forest may lead to ecosystems disruption and reducing natural resources preservation, including reducing ferns species in the forest. Inventory of ferns species need to be performed to gathering information for the preservation and development of the region. The research was conducted from Juni 2015 to September 2015. Ferns sampling was performed on Sebelah Darat, Lingga Village Forest, Sungai Ambawang District. Cruise method was employed to collect ferns. The results were obtained showing that there are 20 kinds of epiphytic and terrestrial ferns dividing of 11 families, Aspleniaceae, Blechnaceae, Davalliaceae, Dennstaedtiaceae, Lindsaeaceae, Lycopodiaceae, Lygodiaceae, Polypodiceae, Gleicheniaceae, Pteridaceae and Selaginellaceae.
ETNOBOTANI TUMBUHAN PANGAN MASYARAKAT DAYAK KANAYANT DI DESA BABANE KECAMATAN SAMALANTAN KABUPATEN BENGKAYANG KALIMANTAN BARAT Megawati, Megawati; Wardoyo, Elvi Rusmiyanto Pancaning; Rafdinal, Rafdinal
Jurnal Protobiont Vol 9, No 2 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i2.45522

Abstract

Food plants is a human need to be met at all times. The pattern of utilization of plants in a society is closely related with their culture. Therefore, the culture of a region will determine the type of foods, the plant parts used, and  the way food is proceesed. The utilization of plants is important for the Dayak Kanayant  tribe especially in fulfilling food needs. The study is carried out for 3 months from June to August 2020.  The study aims to identify  the kinds of  food plants, and their use by Dayak Kanayant in Babane Village.  using the snowball method. The data obtained were analyzed descriptively. The results obtained  were 49 species of food plants,  from 31 families, and the most widely used families were moracaceae and aracaceae, 5 species in each of these families with a percentage of  10.20%. The  widely used  portion of the crop are  fruits with a percentage of  38.09%, while the location of food plants is found more in the forest with a percentage of 41.38%. the way to use it is generally consumed directly and some are processed by sauteed, boiled, burned, and fried.The methods of treating vegetation are boiled, dried in the sun, buried in the ground, added salt and sugar.
Angka Paling Mungkin (Most Probable Number/MPN) Coliform Sampel Kue Bingke Berendam di Pontianak Elvi Rusmiyanto P.W, Dini Agustin, Rahmawati,
Jurnal Protobiont Vol 8, No 1 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i1.30864

Abstract

Bingke berendam cake is one of the traditional foods of Pontianak which is made from rice flour, eggs, sugar and coconut milk. Materials that are used such as sugar, milk and eggs are ingredients that are easily damaged and polluted by microbes. This study aimed to to find out the Most Probable Number (MPN) of coliform bacteria in bingke cakes. Taking bingke cake samples was done at five bingke cake shops in Pontianak city. Isolation of Coliform bacteria used the MPN method, with two stages, namely the estimator test tand the assertive test. Based on the results of this study, the five samples had MPN values ranging from 23 MPN/g to> 100 MPN/g. All samples had MPN values that exceed the threshold so that they did not meet the requirements for PERKA BPOM No. 16 of 2016, which is a 11 MPN/g.
Aktivitas Antibakteri Fraksi Diklorometan dan N-Heksana Paku Sisik Naga (Drymoglossum piloselloides (L) Presl.) Terhadap Bakteri Staphylococcus aureus dan Salmonella typhi Elvi Rusmiyanto Pancaning Wardoyo, Heriyati Siti Khotimah,
Jurnal Protobiont Vol 5, No 3 (2016)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v5i3.17094

Abstract

The potential use of dragon scales have been used by people in Kalimantan as anti-inflammatory drugs, oral thrush and toothache. Dragon scales (D. Piloselloides) is one of epiphytic plants in association with other plants. This study aims to determine the antibacterial activity of the dichloromethane and n-hexane fraction of dragon scales against S. aureus and S. typhi. The research was conducted from October to December 2015 in the Pharmaceutical Chemistry Laboratory Faculty of Medicine, Wood Technology Laboratory Faculty of Forestry, Chemistry Laboratory and Biology Laboratory Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak. Dichloromethane and n-hexane fraction were obtained from the fractionation of methanol extract. Furthermore, both fractions in TLC and chromatogram profiles were visualized using UV 254 and 336 nm. Antibacterial test of D. piloselloides fraction was done by paper disc diffusion method. The concentration used in this study was 0.25; 0.30 and 0.35 g/ml, and as a comparison, Ciprofloxasin 0.005 mg/ml used as a positive control. Antibacterial activity test of dichloromethane fraction of dragon scales has only the ability to inhibit S. aureus, whereas the n-hexane fraction has no antibacterial activity against S. aureus and S. typhi. TLC test results showed that the dichloromethane fraction contains flavonoids compounds, while the n-hexane fraction contains terpenoids compounds.
Profil GC-MS dan Potensi Bioherbisida Ekstrak Metanol Daun Ketapang (Terminalia catappa L.) terhadap Gulma Maman Ungu (Cleome rutidosperma D.C.) Mukarlina, Alia A. Gani, Elvi Rusmiyanto P. Wardoyo,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.19554

Abstract

Ketapang (Terminalia catappa L.) is one of the plants that contains allelochemical compounds which can suppress the growth of surrounding plants that can be utilized as bioherbicide. This research aims to determine the bioactive compounds that have the potential of bioherbicide and the effective concentration of the methanol extract of T. catappa leaf that can inhibit seed germination and growth of purple Cleome (Cleome rutidosperma). The results showed that treatment of the methanol extract of T. catappa leaf can inhibit seed germination and growth of weed C. rutidosperma. The GC-MS spectral data showed that bioactive compounds that have the potential bioherbicide of the methanol extract of T. catappa leaf are Neophytadiene, 3, 7, 11, 15-tetramethyl-2 -hexadecen- 1 ol, 2, 6, 10, 14, 18, 22- tetracosahexaene and Lupeol. The methanol extract of Ketapang (T. catappa) leaf included pre-grown bioherbicide with a concentration of 0.1 g/ml. The research was conducted in Macrobiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences. Evaporation was carried out in the Laboratory of Wood Technology, Faculty of Forestry and the soil chemical content analysis was conducted in Soil Chemistry Laboratory, Faculty of Agriculture, University of Tanjungpura, Pontianak. The Method of Gas chromatography–mass spectrometry (GC-MS) was conducted at the Organic Laboratory of Chemistry Department, Faculty of Mathematics and Natural Sciences, University of Gadjah Mada from August to November 2016. The research used a Completely Randomized Design (CRD) with extract concentrations of 0; 0.1; 0.3; 0.5 and 0.7 g/ml each with three replications.
Inventarisasi Jenis-jenis Tanaman Pekarangan dan Pemanfaatannya di Desa Tanjung Merpati Kecamatan Kembayan Kabupaten Sanggau Rafdinal, Ulul Maulina Riami Elvi Rusmiyanto PW
Jurnal Protobiont Vol 8, No 2 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i2.32476

Abstract

The yard is a plot of land located directly around the house of residence with clear boundaries, planted with one or various type of plants and still have ownership as well as functional relationship with the house concerned. This aims of this study to determine the species of yard plants and their use in Tanjung Merpati Village, Kembayan sub-district, Sanggau districts. The study was conducted from March to May 2017. Location determinations of the study was carried out by purposive random sampling methode which is based on the yard area category, namely large, medium and small categories. Acording to the research finding, there are 89 species of plants belonging to 4 Classes, 34 Orders, and 55 Families. The four classes of plants found were Magnoliopsida, Liliopsida, Gnetopsida and Cycadopsida. Based on the utilization of yard plants found in Tanjung Merpati Village, those plants are divided  into 5 groups, namely ornamental plants, fruit plants, vegetable, medicinal plants and herbs.
Pertumbuhan Semai Bakau Putih (Bruguiera cylindrica (L.) BI.) Pada Tingkat Salinitas Yang Berbeda Elvi Rusmiyanto Pancaning Wardoyo,, Yusniawati, Mukarlina,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20799

Abstract

Salinity increase may effect the growths of several plants, for example (Bruguiera cylindrica (L.) BI.). This research aims to identify the growth of white mangrove seedling (B. cylindrica) on different salinity levels. Additionally, it attempts to recognise salinity that could be tolerated by white mangrove seedling (B. cylindrica). This research was conducted since Oktober to December 2016. It used Completely Randomised Design (RAL) consisting of six (6) treatments, namely control, NaCl 2,5% (S1) treatment, 3% (S2) treatment, 3,5% (S3) treatment, 4% (S4) treatment and 4,5% (S5) treatment. The result showed that NaCl treatments gave a clear effect toward seedling heights, leaf area, root lengths, wet crown weights,dry crown and root weights and chlorophyll content. However, such treatments appeared not to give an effect toward wet root weights. NaCl treatments started to effect growths with the fall of seedling heights, leaf area and root lengths at concentration of 3% and at 4% toward wet crown weights and dry crown and root weights. Meanwhile, the effect toward chlorophyll content was at concentration of 4,5%. Salinity which could be tolerated by B. cylindrica growth stood around 2,5% (25‰) – 3,5% (35‰) and 4% (40‰) for chlorophyll content.
Pertumbuhan Tanaman Bayam Kuning (Amaranthus blitum L.) dengan Pemberian Pupuk Organik Cair Dari Kulit Kecambah Kacang Hijau (Vigna radiata L.) Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina,
Jurnal Protobiont Vol 6, No 3 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i3.22477

Abstract

Yellow spinach (A. blitum L.) is a rarely cultivated plant in West Kalimantan. To overcome this situation, farmers can use Liquid Organic Fertilizer (LOF). This research aims to find out the effects of LOF from The Hulls of Mung Bean Sprouts towards the growth of Yellow Spinach Plant and to find out the concentration of LOF from The Hulls of Mung Bean Sprouts which generates the highest growth on yellow spinach plant. This research was conducted from February to April 2017 in Biology Laboratory of Mathematics and Natural Science Faculty, Tanjungpura University Pontianak. This research used completely Randomized Design which consisted of 5 levels of treatment that are without LOF (control), LOF 55 (P1), LOF 60 (P2), LOF 65 (P3), and LOF 70 ml/L (P4). Each treatment was given 4 times so there were 20 units of experiment. The results show that the giving of LOF with 60 ml/L of concentration offered a significant effect towards all growth parameters. The optimum results of the average height of the plant was 20.17 cm, the number of leaves was 17.25, the shoot wet weight was 6.65 g, the shoot dry weight was 0.69 g, the root wet weight was 16 g, and the root dry weight was 9.50 g.
Co-Authors Abraham Sambo Ahmad Yani Nelly Wahyuni Lia Destiarti Ali Djamhuri Andhini Aprilia Anggraini, Wiwit Ara, Chilliany Ari Hepi Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Ari Widiyantoro Arini Miftahul Jannah Arum Setiawan Arum Setiawan Ary Kusmawati Azkiya, Tiara Catur Wulandari Catur Wulandari Chilliany Ara Cucu Suhery Desy Desy, Desy Devinda Ekarizky Diputri Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Dian Purnama Sari Dwi Gusmalawati Endah Sayekti Ester Yulinda Evi Noviani Fadhlullah, Hafizh Rizqi Firman Saputra Firman Saputra Fransisko, Satria Fuza Amalia Gusrizal Gusrizal Harlia Harlia Hasan Ashari Oramahi Hildayati, Umi Ibnur Rusi Indri Kusharyanti Irtiawati, Irtiawati Irwan Lovadi Junardi Junardi Kardina, Megawati Kustiati Kustiati Kustiati Laila Hanum Lena Dian Saputri Maryati Maryati Megawati - Mukarlina -, Mukarlina Mukarlina, Mukarlina Nurul Huda Oktavia, Vivi Pancaning Wardoyo Peli, Peli Perdana, Aurendyo Yuscel Rachmad Setijadi, Rachmad Rafdinal Rafdinal Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati, Rahmawati Ratu Safitri Renny Puspita Sari Ribka Sinta Uli Panjaitan Rikhsan Kurniatuhadi, Rikhsan Rina agus setiawati Riyandi Riyandi Riyandi Riyandi Riza Linda Riza Linda Rizki Perdana Putri Rousdy, Diah setiawati, Rina agus Sholihin, Nur Siti Hodijah Siti Ifadatin Siti Ifadatin Siti Khotimah Siti Nur Hasanah, Siti Nur Sulistrioningsih, Sulistrioningsih Sumi, Sumi Tri Rima Setyawati Tsuyoshi Yoshimura Turnip, Masnur Unang Supratman Widya Anggraeni Widya Anggraeni Wiwit Anggraini Yudha Arman Zulfa Zakiah