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Edwin Edwin
BINUS University
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Journal : Indonesian Food Science and TechnologyJournal

 1 
Isolation and Identification of Lactic Acid Bacteria Using PCR Gene from Tempe Wrapped with Banana Leaves and Plastic Fevria, Resti; Vauzia, Vauzia; Putri, Dwi Hilda; Achyar, Afifatul; Putri, Santi Diana; Edwin, Edwin
Indonesian Food Science and Technology Journal Vol. 7 No. 2 (2024): Volume 7. Number 2, July 2024 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v7i2.32503

Abstract

Tempe is a typical Indonesian food that comes from fermenting soybeans with the fungus Rhizopus sp. Tempe is known to have good nutritional value because of the content contained in soybeans themselves and other microorganisms that appear as a result of the tempe fermentation process. The fermentation process increases the activity of bacteria in tempe which are beneficial for digestion, one of which is lactic acid bacteria. This research aims to see the differences morphological forms  and genomic of lactic acid bacteria produced by tempe wrapped in banana leaves and tempe wrapped in plastic. The differences in fermentation that occurred in tempe wrapped in banana leaves and those wrapped in plastic resulted in differences in lactic acid bacteria type. Based on the isolation of bacteria on Mann de Rogosa Sharpe Agar medium, 15 isolates of lactic acid bacteria were produced, with general morphological forms of bacilli and coccus. Then, genomic identification was carried out using the PCR. The phylogenetic tree built based on the 16S rRNA gene sequence can show the relationship between LAB diversity at the species level, but cannot differentiate LAB from the strain level.
Co-Authors Achyar, Afifatul Andi Putra Andosy, Dewi Azis, Hendra Sidratul Basyar, Syarifudin Bernard Bernard, Bernard Budhi, Reza Santya DAW, Martinus Diah Arimbi Dwi Arkam, Fadhil Dwi Hilda Putri Dwi Putra, Jevon Agustinus Efrila, Efrila Erick, Erick Eufemia, Eufemia Evan Azra Wafa Simamora Fahmi Rustan, Muh Fevria, Resti Fitri Yanti Frendy Bong, Frendy H.M. Edwar Juliartha Halimatus Syahdia Hasibuan Hardani Widhiastuti Haryadi Sarjono Himawan Sentosa, Himawan I Gusti Putu Suharta I Putu Sriartha I Wayan Lasmawan I Wayan Widiana Jeslyn, Jeslyn Karjunita, Nike Ken Ratri Retno Wardani Lumunon, Lumunon Mardalena , Mardalena Mau, Stefanus Dwi Istiavan Monica , Indiastri P Moralita Chatri Muhammad Parikesit Wisnubroto Nasser, M. Niluh, Niluh Novita Wulandari Ong, Jovi Pesik, Heidy Prastopo, Prastopo Probowati, Dina Puspita Purnamasari, Devi Purwandari, Maya Febriyanti Putri, Santi Diana Rafli Rasyid, Muh Rahmi, Wifqi Ricky Banke Sabawaly, Diana Reby Sahary, Fitry Taufik Saputra, Renaldi Eka Sen, Thio Yan Setiawi, Agustina Purnami Shita, Shita Silitonga, Vera Dumonda Sitompul, Frans Della Sri Heriza Suhendra, Dede Sukma Sahadewa, Sukma Suprapto, Yandi Tantra Wijaya, Antonio Tanudjaja, Daniel Januar Tjahjana, Barah Tumiwa, Kiet Vauzia Vauzia, Vauzia Wisnubroto, Muhammad P Wulan Kumala Sari Yulistriani, Yulistriani Zahlul Ikhsan