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Isolation and identification of biosurfactant producing bacteria from wastewater treatment pool of Bumi Siak Pusako – Pertamina Hulu Ltd, Siak Sri Ayu Elsi Maita; Hasbi Hasbi; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract Biosurfactants are surfactant compounds produced by microorganisms and it has capability to reduce surface tension of water. To understand the types of biosurfactant producing bacteria,a research has been conducted in March 2018. Samples were taken with glass bottles at 6 sampling points in the wastewater treatment pool of Bumi Siak Pusako – Pertamina Hulu, Ltd and stored on the refrigerator at 4oC.  The  samples were serially diluted and enriched with BHI (Brain Heart Infusion) media for 24 hours. Then  this bacteria was identified biochemically and using a 16 sr RNA method. Results shown that the number of colonies obtained was 3.1 x 106 cfu/ml. After the emulsification test, 3 isolates were able to produce foam, they were Klebsiella sp, Citrobacter sp and Aeromonas sp. Keywords : surface tension, Klebsiella sp, Citrobacter sp, Aeromonas sp, 16 sr RNA, emulsification
Effect Of Bioijuk Density On Decreasing TSSIn Sail River Water Prisusan Permata Hati; Budijono Budijono; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Sail River is one of the Siak watersheds that have been polluted due to high levels of TSS. This study aims to determine the level of reduction in TSS levels by using different bioijuk densities. This research was conducted in March-April 2019. The method used was a completely randomized design (CRD), which was used as treatment, was bioijuk density. P¬1 (infrequent density), P2 (medium density) and P3 (meeting density), and 3 replications. The sampling location is selected in the inlet and outlet area. The results of the measurement of the average reduction in the highest TSS levels were found in P2, 207 mg / L with a reduction in effectiveness (EP) of 97%. From the results of the study, it was known that the density of different bioijuk did not significantly influence the decrease in TSS, levels in the Sail River water.Keywords : Bioijuk, Continuous Solids, Dissolved Solids, Turbidity, Bacteria
SUSPENSION OF THE SUSPECT IN SAIL RIVER WATER PEKANBARU CITY Intan Ratna Purba; Budijono Budijono; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT This study aims to determine the value of suspended solids contained in in the waters of the Sail River. This research was conducted in March-April 2019. Load suspended solid samples were taken from three stations, namely at the upstream part (Station 1), the middle part (Station 2) and the downstream (Station 3). Sampling is done three times, and every 10 days sampling is done. The results obtained are the value of suspended solids content ranging from 68-332 mg/L. Thus the Sail River waters have been contaminated by suspended solids. Keywords: Suspended solids, Sail River
The effectiveness of biofilter to reduce the level of nitrate and phosphate content in the butchery liquid waste Rika Vebriyanti Br Ginting; Sampe Harahap; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract The butchery liquid waste is rich in nitrate and phosphate and it need to be processed before being flown to the environment. To understand the effectiveness of combined anaerob-aerob biofilter in reducing the nitrate and phosphate content in the butchery liquid waste, a study was conducted in  March – April 2018. The waste (315 L) was treated using a batch system that was consisted of 2 aerob and 2 anaerob tanks. The butchery liquid waste was kept for 10 days in anaerobtanks, 7 days in aerob tanks. By the end of the experiment, the nitrate reduced from 39 mg/l to 12.4 mg/l (the effectiveness was83.21%). While the phosphate reduced from 15.4 mg/l to2.9 mg/l (the effectiveness was 81.16%). Other water quality parameters such as pH was normal and DO was improved (from 1 mg/L to 5 mg/L). The treated waste was used as a mediafor culturingH. pluvialis. Observations of H. pluvialiswere conductedfor 8 days. The H.pluvialis density peaked by the 6th day, it was963,33 cells / ml.  Based on data obtained, it can be concluded that the useof anaerob -aerob biofilter was effective forimproving the butchery waste water quality . Keyword : Organic waste, Anaerob tank, Aerob tank, Haemotococcus pluvialis 
The EffectivenessOf BiorafiaIn Reducing Biological Oxygen Demand (BOD5) Content In The Sail River’s Water Rizka Novtri Andini; Budijono Budijono; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTOne of the pollutions that enters the Sail River is organic pollutants derived from domestic waste. Efforts to reduce organic pollutants can be conducted by using biocords. Biocord is a rope in the form of three-dimensional fine fiber webbing that is able to hold microorganisms in large quantities. This research used biorafia media (material made from raffia-based materials). The method used was CRD with 3 different biorafia densitieslow density (P1), medium density (P2) and  high density (P3) with 3 replications each. Biorafia is placed in a ditch drained from the Sail River. Biorafia functions as a place attached to microorganisms (biofilms). The degradation process of organic matter occurs when in contact with biofilms that are present in biorafia. The purpose of this study was to reduce the levels of BOD5. The results showed a decrease in BOD5 levels with a time interval of 1 month. The effectiveness of the decrease in the average BOD5 was P1 71,4,%, P2 74,1% dan P3 69,5%. Keywords:biocord, biorafia, organic pollutants, microorganisms 
The effectiveness of palm oil liquid waste enriched media on growth of Chlorella sp. in the outdoor scale Febriana Nur Vitriani; Budijono '; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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The liquid waste of palm oil containing high nutrients and can be used as a source of nutrients for growth microalgae such as Chlorella sp. A research aims to understand the effects of palm oil liquid waste addition in the media toward the growth of Chlorella sp. has been conducted from May – July 2016. The culture was conducted for 20 days. There were 4 treatment applied, namely P0 (0% waste), P1 (20% waste), P2 (25% waste) and P3 (30% waste). Parameters measured were the abundance and biomass of Chlorella sp., temperature, pH, nitrate, and phosphate. Result shown that the best growth of Chlorella sp. was in the P1, the abundance was 61.45 × 105 cells/ml and the biomass was 0.36 g/l. During the research, the nutrient available in the media decreased, from 1.13 to 0.09 mg/l (nitrate) and from 3.54 to 0.48 mg/l (phosphate). This fact indicates that the nutrient have been used for growing the microalgae. Based on data obtained, it can be concluded that the palm oil liquid waste can be used as a source of nutrients for Chlorella sp.Key words : Palm oilliquid waste, nutrients, microalgae, Chlorella sp.
The Effectiveness of EM4 Addition in the Biofilter Tanks to Reduce TSS and Ammonia in the Sago Industry Liquid Waste Mila Suryani Pohan; Sampe Harahap; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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                                     AbstractSago liquid waste is rich in TSS and amonia and thus need to be processed before being flown to environment. A study aims to reduce the TSS and amonia in that waste by using the biofilter that was added with EM4has been conducted in April-May 2019. There were 3 treatments applied, namely the addition of 17 ml, 20 ml and 23 ml EM4in the anaerob and aerob tanks, 3 replications. The waste was settled for 3 days in the anaerob and 3 days in the aerob tanks. The treated waste was then tested for TSS and ammonia concentrations. The treated waste was also used for rearing Oreochromis niloticus seedlings. Results shown that after being treated using the EM4enriched biofilter the TSS reduced into 80 – 32 mg/L and amonia 2,6329– 1,0420 mg/L. The survival of Oreochromis niloticus seedling reared in the treated waste was 86-93%. The best treatment to reduce the TSS was the addition of 20 ml EM4and the effectiveness was 99%, while that of the ammonia was 90%.It can be concluded that the addition of EM4to the biofilter tanks was able to reduce the TSS and ammonia in the sago industrial liquid waste.   Keywords: Aerob  tank, Anaerob tank, Effective microorganism 4, Oreochromis niloticus
Isolation and Selection of Biosurfactan Producing Bacteria from the Pond in the Gathering Station EOR Plant PT. Bumi Siak Pusako Pertamina Hulu Riau Province Sardion Simatupang; M.Hasbi M.Hasbi; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract The water in the pond at PT Bumi Siak Pusako-Pertamina Upstream Eor Plant Gathering Station is polluted by oil. The presence of oil in pond water triggers gene mutations in bacteria so that the bacteria can produce biosurfactants to degrade oil. The aim of this study was to obtain a biosurfactant-producing bacterial isolate and was carried out in January - February 2019. Bacterial isolation was started by taking 5 ml of pool water samples and mixed with 40 ml of Bacterial Fertilizing media namely Brain Heart Infusion (BHI) and then incubated for 1 x 24 hours . Then the bacteria are grown using Mac conkey media. Pure bacteria are biochemically tested and emulsified. Morphological and biochemical characteristics show that 2 species of bacteria, namely Provindensia sp and Citrobacter sp. Emulsification index results show that all bacteria obtained have the potential to produce biosurfactants.Keywords: Providensia sp, Citrobacter sp
Isolation and Selection of Biosurfactant-Producing Bacteria from Sludge Siak River, Around Sungai Duku Port of Pekanbaru Vera Linda Uli Simanjuntak; M. Hasbi M. Hasbi; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractActivities in the Sungai Duku port was causing oil pollutionwhich then trigger gene mutation in specific bacteria.  The bacteria may produce biosurfactants for degrading the oil. A study aims to obtain the biosurfactantproducingbacteria from oil polluted sludge was conducted fromJune to July 2018. Thesludge sample was taken to laboratoryand 2 ml of the sample was mixed with Enrichment media to grow up the bacteria. The bacteria were isolated and purified using Blood Agar and MacConkey media. The purified bacteria were then morphologically studied and biochemicallytested. Morphological and biochemical characteristics shown that there were 2 bacteria species, namely Proteus sp and Enterobacter sp.Emulsification Index of the Proteus sp was 42.5% and  Enterobacter sp was 50%, indicating that those bacteria were potencial for producing biosurfactant.Keywords : Enterobactersp, Proteus sp, Biodegradationt, Oil pollutant, Emulsification Index
The effectiveness of anaerob-aerob biofilter and phytoremediation using Salvinia molesta for reducing phosphateand MBAS (Methylen Blue Active Substance)content in laundry liquid waste Eko Saputra; Sampe Harahap; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract The laundry waste is rich in MBAS and phosphate and its need to be processed before being flown to environment. This study aimsto determine the ability of  abatchbiofilter system in reducingthe phosphate and MBAS content. This study has been conducted in January-March 2018.The laundry liquid waste (175 liters) was kept in 2 anaerobik tanks that was completed with gravel, sand and palm fibers media for ten days.Then the waste was flown to aerobik tanks (coconut shellcharcoal media) for seven days. Finallythe wastewas treated in a phytoremediation pondthat was completed withSalvinia molestafor fifteen days. By the end of the experiment, the phosphate reduced from 11.6-0.8 mg/L (the effectiveness was 92.67%)and the MBAS reduced from 10.5-1.3 mg/L (the effectiveness was 87.16%). Other water quality parameters such as pH was normal and temperature and DO were improved. The treated waste was then used for rearing Cyprinus carpio (5-7 cm TL) for 10 days. The survival rate of the fish was 89%. Based on data obtained, it can be concluded that the combination of biofiters and phytoremediation system using Salvinia molesta is effective to reduce the phosphate and MBAS content in the laundry liquid waste. Keyword:waste water, detergent contaminated waste, bacth system, surfactant 
Co-Authors Adhe Permana Adhistya Agung Wijayanto Agustin Widyowati Aji Santoso Alit Hindri Yani Alwin Ferdinand G Amma Fizka Zakiyatul Miskiya Antonius Bimo Rentor Apriani Adha Nasution Arni Damayanti Asra Trigana Lisva Atina, Vihi Ayundha Evanthi Budijono, Budijono Bunga Citra Lestari Daghista Putri Pawestri Dahlila Hidayani Dede Nur Zaman Deni Efizon Desyandri Desyandri Diana Novira Dwi Rahmawati Egan Evanzha Amriel Eko Febrianda Eko Saputra Endah Ratnawati Eri Febriansyah Erni Yuliani Ertien Rining Nawangsari Ery Permana Yudha Esti Mardiana Evi Iskana Manurung Faisal Aji Syahputra Fajriah Khairunnisaa Febriana Nur Vitriani Ferdiana Rossi Ferdina, Cucun Setya Fika Fitriani Fioga Zahra Fernando Firman Adi Candra Firman Arifandi Firman Dwilaksono Rahardianto Fitriana Sinaga Fitrianis Yulanda1 Geliska Wahyuni Gulo, Hamer Dekari Gunawan Tanuwidjaja Hafidz Munawar Kholil Hani Magfiroh Nur Ratmah Hany Arya Wardhany Hariyawati, Susi hasbi hasbi Herman Syahputra Tambunan Ida Muidah Ika Putri Pujianti, Ika Putri Pujianti Indah Wahyu Utami Intan Ratna Purba Ira Wikartika, Ira Irawan, Ridwan Dwi Irma Pramudhita Ismail Mubarok Iyus YL Tobing Johannes Kristian Siregar Julia Rastifa Dewi Kaca Dian Meila KHAIRUL ANWAR Khairunnisa Karimah Kholifia A. Muthmainah Kholifia Ainun Muthmainah Kusrini Kusuma Ratnawati Laksono Trisnantoro Lovi Yona M Hasbi M Hasbi M Iqbal Zarkasyi M. Hasbi M. Hasbi M.Hasbi M.Hasbi M.S. Idrus Made Sudarma Masharin Mila Suryani Pohan Mira Kurnia Irde Mirza Shahreza Muhadjir Anwar Muhammad Arib Faishal Muhammad Fahmi Panwar Muhammad Fitra Muhammad Ilyas Muhammad Razi Ridha N Heriyah Nabila Arumdani Nabila Ghufraeni Rona Raharjo Nazla Keyla Nibras Fa’iq Muhammad Novita Puspitasari Nurchim Nurmalitasari Permatasari, Hanifah Pipin Widyaningsih Prasetyohadi Prasetyohadi Prisusan Permata Hati Putri Gantine Lestari Putri Laili Susanti Raden Mohamad Herdian Bhakti Raihan Cakra Haryudin Ramos Gunawan Randis Wahyuni Ranggi Efendi Rapih N. Kharismatika Rapih Nur Kharismatika Ratna Melinda Putri Rifka Hendarini Putri Rika Vebriyanti Br Ginting Riski Dwi Putri Riski Dwi Putri Rizka Novtri Andini Rizki Sahrul Barokah Rizki Syawalia Siregar Ronika Butar butar Roy Astika Simatupang Salma Juliana Fadilah Sampe Harahap Sardion Simatupang Septian Aristianto Setiawan, Muhammad Ricky Setrayana, Abiyyu Siska Danawati Sri Ayu Elsi Maita Sri Nefrbina Bangun Sri Widata Sulastri Yenita Putri Tantry Widiyanarti Telaga Nabila Putri Riyanto Toto Hartono Tutus Setiawan Vallesa Archika Fany Vera Linda Uli Simanjuntak Widiyanto, Wahyu Wijaya Witandri Wulan Nur Layli Yacobus Sunaryo Yudi Ferdian Situmeang Yunarto, Atung Zumrotul Fitriyah