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PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10 Ainutajriani, Ainutajriani; Darmawati, Sri; Zilda, Dewi Seswita; Afriansyah, Muhammad Ardi; Saptaningtyas, Ragil; Ethica, Stalis Norma
BIOTROPIA Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1765

Abstract

Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.
Co-Authors A.A. Ketut Agung Cahyawan W Abdillah, Rifqi Aditya Rahman Ernanto Aditya Rahman Ernanto Aditya Rahman Ernanto Afriansyah, M. Ardi Afriansyah, Muhammad Ardi Agus Sabdono Agus Suyanto Agus Suyanto Aida Zakiyatul Fikriyah Ainutajriani Ainutajriani Ainutajriani, Ainutajriani Alisha Triwahyuni Alvira Intar Bella Cahya Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Ana Hidayati Mukaromah Andri Sukeksi Aprianti, Nurannisa Fitria Aprilia Indra Kartika, Aprilia Indra Arfiyanti, Mega Pandu Arham, Chaerul Aufit Fahima Aulia Harun Aulia Meirizka Saputra Ayanti, Bio Putri Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Cendani, Tinezia Rivan Dewi Seswita Zilda Dhea Ayu Lestari Dian Purwo Jatinging Putri Diatri, Devita Dilin Rahayu Nataningtyas Diyanah, Dina Dwi Pamaya Elly Rustanti Ellyka Purwaningrum Endang Semiarti Endang Semiarti Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Fanda Indriyani Fandhi Adi Wardoyo Farabi, Meka Faizal Fauziyyah, Dinah Febrianti, Pepi Feri Feri Fitrah, Reza Fuad, Hayatun Hayatun Fuad Hikmah, Annisa Nurul Ika Dyah Kurniati Istini Istini Izza, Naimatul JAKA WIDADA Jaka Widada Janah, Inayah Qori Nur Jimat, Resi Tondho Jumardi, Muhamad Kazi Mohammad Zillur Rahman Khoirunisa, Asiva L. Hartanto Nugroho Lestari, Citra Rahayu Maya Dian Rakhmawatie Maya Linda Shafira Meturan, Dessy Marselina Meyliana, Adinda Mirza, Sa’if Revallindo Mudyawati Kamaruddin Muhammad Ardi Afriansyah Muhammad Ardi Afriansyah Mukarromah, Ana Hidayanti Musa, Susan Hasanah Mutia Srikandi Fitria Nanik Marfu'ati Ningsi, Ana Lestari Noverson Lidaya Nur Hidayah Nur Hidayah Nur Hidayati Nur Hidayati Nurcahyono Nurcahyono Nurcahyono Nurcahyono, Nurcahyono Nurrahman Nurrahman Nurrahman Nurrahman Nurrahman Nurrahman Oedjijono Oedjijono, Oedjijono Okta Yosiana Dewi Pratama, Aji Sukma Puji Lestari Putri, Vivi Vahtalava Radna Safitri Rafiyanto, Achmad Ragil Saptaningtyas Ragil Saptaningtyas Ragil Saptaningtyas, Ragil Richard David Silvestre Riswan Riswan Rivana Ariyadi Rosyida Azis Rizki Sakti Imam Muchlissin Samiroh Samiroh, Samiroh Setia Iriyanto Setia Iriyanto Silvestre, Richard David Siska Nurprihandayani Siti Aminah Siti Aminah Siti mutmainnah Sofyan Yosias Beslar Sri Darmawati Sri Darmawati Sri Darmawati Sri Elvira Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi suardi suardi Sukowiyono Sukowiyono Sukowiyono Sukowiyono Sulistyaningtyas, Ayu Rahmawati Sultistyaningtyas, Ayu Rahmawati Tamimi, Iqbal Theresia Liyana Sejati Tri Joko Raharjo Tri Joko Raharjo Viandy, Amartya Desta Vicky Mahendra Nurzhulian Vradea Pramesta Wa Ode Inayatul Wahyuni, Arti Widyani, Dhiyananda Wikanastri Hersoelistyorini Wikanastri Hersoelistyorini Yuni Nurkuntari Zakiya, Roma Dhona Beauty