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All Journal International Journal of Evaluation and Research in Education (IJERE) Jurnal Pendidikan Sains Techno.Com: Jurnal Teknologi Informasi Jurnal Ilmiah Mahasiswa FEB Biotropika Prosiding Seminar Biologi Jurnal Pendidikan Biologi Indonesia MIX : Jurnal Ilmiah Manajemen PENA SAINS Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Ekonomi Bisnis Scholaria: Jurnal Pendidikan dan Kebudayaan BIOEDUKASI Jurnal Dinamika Ekonomi dan Bisnis Biosfer: Jurnal Pendidikan Biologi Bioedukasi: Jurnal Pendidikan Biologi Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Biota Jurnal Eduscience (JES) Jurnal Karinov Abdimas UNWAHAS : Jurnal Pengabdian Masyarakat UNWAHAS Penbios: Jurnal Pendidikan Biologi dan Sains Sains dan Matematika Journal of Biological Science and Education JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) Bioscientist : Jurnal Ilmiah Biologi Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmiah Wahana Pendidikan Proceeding Biology Education Conference TEKIBA : Jurnal Teknologi dan Pengabdian Masyarakat Jurnal Ilmu Manajemen, Ekonomi dan Kewirausahaan ABDINE Jurnal Pengabdian Masyarakat Research and Development in Education (RaDEn) Journal of Biological Science and Education Jurnal Kedokteran Hewan Jurnal Kajian Penelitian Pendidikan dan Kebudayaan Jurnal Manusia dan Lingkungan Berkala Ilmiah Biologi Al-Kharaj: Jurnal Ekonomi, Keuangan & Bisnis Syariah Nomico Jurnal Ilmu Hayat Biodik: Jurnal Ilmiah Pendidikan Biologi Konstruktivisme : Jurnal Pendidikan dan Pembelajaran Jurnal Biosilampari: Jurnal Biologi
Fatchur Rohman
Universitas Negeri Malang
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Journal : Jurnal Manusia dan Lingkungan

 1 
EFEK PERLAKUAN LOGAM BERAT KADMIUM TERHADAP APOPTOSIS MELALUI AKTIVASI CASPASE-3 BULU BABI Deadema setosum: APLIKASI BIOMONITORING PENCEMARAN DI PERAIRAN LAUT (Effect of Cadmium Heavy Metal Treatment Toward Apoptosis Through Caspase-3 Activation of Sea) Dominggus Rumahlatu; Aloysius Duran Corebima; Mohamad Amin; Fatchur Rohman
Jurnal Manusia dan Lingkungan Vol 21, No 1 (2014): Maret
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18510

Abstract

ABSTRAKKadmium yang terakumulasi pada hewan dapat menyebabkan karsinogenik, mutagenik, dan teratogenik. Pada penelitian ini, dilakukan secara eksperimen nonfaktorial dalam RAL 6 level dengan 7 ulangan selama 4 minggu, untuk mengkaji efek perlakuan logam berat Cd terhadap apoptosis melalui aktivasi protein caspase-3 bulu babi Deadema setosum. Penelitian dilakukan di laboratorium Balai LIPI Ambon dalam 6 bak aquarium berukuran 100 x 60 x 70 cm3.Tiap bak perlakuan diisi 200 L air laut yang diganti satu kali setiap minggu. Konsentrasi perlakuan adalah 0, 1, 3, 6, 9, dan 12 µg/L Cd terlarut. Pada tiap bak diterapkan satu level perlakuan konsentrasi Cd, dan tiap bak itu dihuni oleh 7 individu D. setosum sebagai ulangan. Usia hewan uji sekitar 8 bulan berbobot 90 g dengan lingkar tubuh 15 cm. Hewan uji diberi pakan lamun. Pengukuran konsentrasi protein caspase-3 dilakukan pada organ hepar dengan metode Caspase Colorimetric Assay Kit, sedangkan pemeriksaan apoptosis dilakukan dengan teknik pengecatan Hematoxilen-Eosin (HE). Data penelitian terkait konsentrasi protein caspase-3 dianalisis dengan One Way Anova dan uji lanjut dihitung dengan Duncan 0,05. Hasil penelitian memperlihatkan bahwa perlakuan Cd sangat signifikan meningkatkan kadar protein caspase-3; semakin tinggi kadar Cd, kadar protein caspase-3 juga makin tinggi. Konsentrasi protein caspase-3 pada konsentrasi perlakuan 12 µg/L Cd adalah yang paling tinggi dibanding kontrol. Gambaran histologi hepar D. setosum yang mengalami apoptosis atas dasar pengecatan HE sangat sesuai dengan tiap konsentrasi protein caspase-3 yang terekam. Hasil ini menunjukkan bahwa protein caspase-3 memiliki potensi sebagai satu alternatif biomonitoring pencemaran Cd pada tingkat seluler D. setosum di perairan laut.  ABSTRACTCadmium which accumulated in animals could cause carcinogenic, mutagenic and teratogenic. In this research, non factorial experiments conducted in Complete Random Design (CRD) 6 level with 7 replication for 4 weeks, in order to examine effect of Cd heavy metal treatment toward apoptosis through caspase-3 protein activation in sea urchin Deadema setosum.  Research conducted in LIPI Ambon laboratory using 6 aquarium tank size 100 x 60 x 70 cm3. Each tank was filled with 200 litres of sea waters that being replace once a week. Treatment concentrations in tanks  are containing 0.0, 1.0, 3.0, 6.0, 9.0, and 12.0 µg/L dissolved Cd. In each tank there is one level Cd concentration treatment level, and each tank was filled with 7 Deadema setosum individual as replication. The age of animals is approximately 8 month with the weight 90 gram and body circumference 15 cm. These animals were fed with seagrass. Measurement of Caspase-3 protein concentration was conducted in heparin organ using the method Caspase Colorimetric Assay kit, while apoptosis examination was conducted by painting technique using Hematoxilen-Eosin (HE) in Physiology and Histology Laboratory of Medical Faculty Brawijaya University. Research data concerning caspase-3 protein concentration was analyzed by One Way Anova and further test was calculated by  Duncan 0.05. Result of this research showed that Cd treatment is significantly increasing caspase-3 protein content; higher Cd content, caspase-3 protein would essentially higher. Caspase-3 protein concentration in treatment concentration of 12 µg/L is the highest compared to control treatment. Histological description of Deadema setosum heparin organ that experiencing apoptosis based on HE painting is in accord with each recorded caspase-3 protein concentration. This result showed that caspase-3 protein has the potential to become one alternative in Cd pollution biomonitoring at celluler level of Deadema setosum in the sea. 
Co-Authors Aan Zainul Anwar Adelina Rosidi Adiansyah, Romi Agil Lepiyanto Agnes Melani Agung Witjoro Agus Dharmawan Agus Irwanto Ahmad Yani Akhmad Fathoni Akrifa Nindya Damayana Alfany Abied Maulana Satmaka Alkhaf Permana Aloysius Duran Corebima Aloysius Duran Corebima Aloysius Duran Corebima Aloysius Duran Corebima Aloysius Duran Corebima Aloysius Duran Corebima Amelindha Vania Ananda Sabil Hussein Arif Rahman Arifin, Arifah Novia Arini Zahrotun Nasichah Ary Maf’ula Asmaul Lutfauziah Atik Kurniawati Ayu Tri Wedyastuti Ayuni Berta Anugrahsari Azzakiyatul Amaliyah Bagus Priambodo Bagyo Yanuwiadi Bidayatul hidayah Budy Wiryawan Chairina Kusumaningrum Choirunnisa', Lely Choirunnisa’ Ainur Rohmah Daffa Rizkidhiya Hanif Danial Mursyd Dianatur Raviqah Diemas Nurwikan Din Hadi Shofyan Dominggus Rumahlatu Duran Corebima Aloysius Dwi Iriani Eka Ariyati Endah Sulistyowati Endang Suarsini Erna Wijayanti Farid Akhsani Fauzia Rahma Syaharani Febriani Sarwendah Asri Nugraheni Gadis Triyani Rasyiida Habiburrohman Aksa Hadi Suwono Hanif Hanif Hanum Isfaeni Hedi Sutomo Hedi Sutomo Hendra Susanto Herawati Susilo Herlina Herlina Husamah Husamah I Putu Oktap Indrawan Ibrohim Ibtagh Kulla Ma’ruf Ida Purwami Ismawan Hariadi Istamar Syamsuri Isyfa Fuhrotun Iwan, Iwan I’in Roikhatun Nuzulul Ilmi Jessy Damayanti Kahfi Munandar Khabib Solihin Khofifatu Nurisya Kiki Taurista Kistantia Elok Mumpuni Laila Maghfiroh Lailatul Maghfiroh Laily Asfaniy Larasati Ayu Sekarsari Latuheru, Anita M. Ravvi Dinil Adhim Maisuna Kundariati Maisuna Kundariati Maisuna Kundariati Maisuna Kundariati Marinda Sari Sofiyana Mega Ayu Putri Hapsari Megah Sri Retno Apriliana Miftahul Hasanah Mimien Henie Irawati Mochammad Yordan Adi Pratama Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Muh Fachry Ambia Muhamad Bilal Muhammad Harun Ar Rosyid Muhammad Iskandar Sjah Muhammad Mifta Fausan Muhammad Ridho Maulana Muhammad Rijal Muhana Gipayana Murni Ramli Murni Sapta Sari Mursyd, Danial Mutholingah Mutiara Nesya Kasami Rahayu Yohanna Ninik Kristiani Nizar Azizatun Nikmah Noermijati . Noor Azean Atan Novanda Sonia Kasih NUR LINA SAFITRI Nur Maf’ulla, Putri Nur Uswatul Khairah Nur Wulan Tri Wakhida Nurwidodo Nurwidodo Okta Qomaruddin Aziz Pasalbessy, Victor F. Qadariah, Nosi Rahmadyah Kusuma Putri Raihan Ahlul Firdaus Randy Ramadhan Ratri Rachmawati Ratri Rahmawati Rendy Gusti Radityatama Restu Fitriani Reza Ardiansyah Rimbi Paulina Dewi Riri Rahmadani Putri Risca Dwi Kusuma Rizhal Hendi Ristanto Rizmia Dewi Rosari, Dina Rovila El Maghviroh Ruchimah Achmad Sadiah, Mahmudah Saidil Mursali Salha Hamidah Samuel Agus Triyanto Sayidatun Nisa Sunjaya Septavian Triarif Pamungkas Setiyo Prajoko Setyoko Setyoko Sheryl Chintya Lena Siburian, Cindy Noviana Erintan Silfia Ilma Silfia Ilma Siti Imroatul Maslikah Siti Zubaidah Sitoresmi Prabaningtyas Sitti Saenab Sofia Ery Rahayu Sri Endah Indriwati Sri Rahayu Lestari Subadriyah, Subadriyah Subhan Maulidi Kun Bad'a Farohii Sueb Suhadi Suhadi Sulisetijono Sulisetijono Sumiati Sumiati Sumiati Sumiati Suparno Putera Makkadafi Susriati Mahanal Susriyati Mahanal Sutiman Bambang Sumitro Syelvi Puspitarani Syukron Miftakhul Khoiri Tesa Manisa Turista, Dora Dayu Rahma Utami Sri Hastuti Vipta Nur Riza Vivi Novianti Wachidah Hayuana Widya Sartika Sulistiani, Widya Sartika Yuli Chasanah Yusri Juma Zainal Abidin Zakiyatus Sarifah Zia Aulia Zaidin Putra Zurienia Mimi Bibiyana